The effect on meiotic synapsis of a recombination modulator in Petunia hybrida

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 443-453 ◽  
Author(s):  
M. Abirached-Darmency ◽  
E. Tarenghi ◽  
J. H. de Jong

In Petunia hybrida, a major gene called Rm1 is able to induce a considerable increase in the meiotic recombination rates. To monitor the effect of Rm1 on meiotic synapsis, synaptonemal complex (SC) formation was investigated in pollen mother cells of Rm1 Rm1 and rm1 rm1 plants. Three-dimensional serial-section reconstruction showed no morphological difference in their SC structure. In two-dimensional spreading nuclei, although extensive SC formation was observed in the absence of Rm1, our observations strongly suggest that SC formation is more regular and more efficient in the presence of Rm1. SC analysis showed that the reduced chiasma frequency observed in rm1 rm1 plants corresponds to a partial failure of synapsis involving chromosome ends, pericentric regions, and may also extend to the whole length of short chromosome arms. It is proposed that Rm1 probably corresponds to a regulatory system with multiple effects, including the course of synapsis and the extent and quality of SC formation.Key words: synaptonemal complex, synapsis, chiasma, recombination modulator gene, petunia.

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 867-873 ◽  
Author(s):  
R Banerjee ◽  
G H Jones

The model cytogenetic plant species Crepis capillaris (2x = 6), in which all 3 chromosomes are readily distinguished, was used to analyse the initiation and progression of meiotic synapsis in a large sample of spread and silver-stained pollen mother cells. Particular emphasis was placed on detecting general patterns or trends of synaptic order, both among different bivalents and within (along) individual bivalents, and investigating the consistency or otherwise of these synaptic patterns. The order of synaptic progression and completion was partly related to chromosome length; as in other species, shorter bivalents tended to complete synapsis ahead of longer ones. Individual bivalents also showed distinct patterns of synapsis, with a tendency for subterminal regions to initiate synapsis early, followed by multiple synaptic initiations in internal bivalent regions. However, the analysis showed that these synaptic patterns are only general trends and significant variations in synaptic order and pattern, among and within bivalents, occur in individual cells.Key words: meiosis, synapsis, synaptonemal complex, Crepis.


Author(s):  
S. Khadpe ◽  
R. Faryniak

The Scanning Electron Microscope (SEM) is an important tool in Thick Film Hybrid Microcircuits Manufacturing because of its large depth of focus and three dimensional capability. This paper discusses some of the important areas in which the SEM is used to monitor process control and component failure modes during the various stages of manufacture of a typical hybrid microcircuit.Figure 1 shows a thick film hybrid microcircuit used in a Motorola Paging Receiver. The circuit consists of thick film resistors and conductors screened and fired on a ceramic (aluminum oxide) substrate. Two integrated circuit dice are bonded to the conductors by means of conductive epoxy and electrical connections from each integrated circuit to the substrate are made by ultrasonically bonding 1 mil aluminum wires from the die pads to appropriate conductor pads on the substrate. In addition to the integrated circuits and the resistors, the circuit includes seven chip capacitors soldered onto the substrate. Some of the important considerations involved in the selection and reliability aspects of the hybrid circuit components are: (a) the quality of the substrate; (b) the surface structure of the thick film conductors; (c) the metallization characteristics of the integrated circuit; and (d) the quality of the wire bond interconnections.


Author(s):  
B. Carragher ◽  
M. Whittaker

Techniques for three-dimensional reconstruction of macromolecular complexes from electron micrographs have been successfully used for many years. These include methods which take advantage of the natural symmetry properties of the structure (for example helical or icosahedral) as well as those that use single axis or other tilting geometries to reconstruct from a set of projection images. These techniques have traditionally relied on a very experienced operator to manually perform the often numerous and time consuming steps required to obtain the final reconstruction. While the guidance and oversight of an experienced and critical operator will always be an essential component of these techniques, recent advances in computer technology, microprocessor controlled microscopes and the availability of high quality CCD cameras have provided the means to automate many of the individual steps.During the acquisition of data automation provides benefits not only in terms of convenience and time saving but also in circumstances where manual procedures limit the quality of the final reconstruction.


2017 ◽  
pp. 83-99
Author(s):  
Elisabetta Mafrolla ◽  
Viola Nobili

This paper investigates whether and at what extent private firms reduce the quality of their accruals in order to signal a better portrait to the bank and obtain new or larger bank loans. We measure earnings discretionary accruals of a sample of Italian private firms, testing whether new and larger bank loans are associated with a higher (lower) quality of earnings in borrowers' financial reporting. We study bank loan levels and changes and how they impact discretionary accruals and found that, surprisingly, private firms' discretionary accruals are systematically positively affected by an increase in bank loans, although they are negatively affected by the credit worthiness rating assigned to the borrowers. We find that the monitoring role of the banking system with regard to the adoption of discretionary accruals is effective only when the loan is very large. This paper may have implications for policy-makers as it contributes to the understanding of the shortcomings of the banking regulatory system. This is an extremely relevant issue since the excessive amount of non-performing loans held by Italian banks recently threatened the stability of the European Banking Union as a whole.


1990 ◽  
Vol 18 (4) ◽  
pp. 216-235 ◽  
Author(s):  
J. De Eskinazi ◽  
K. Ishihara ◽  
H. Volk ◽  
T. C. Warholic

Abstract The paper describes the intention of the authors to determine whether it is possible to predict relative belt edge endurance for radial passenger car tires using the finite element method. Three groups of tires with different belt edge configurations were tested on a fleet test in an attempt to validate predictions from the finite element results. A two-dimensional, axisymmetric finite element analysis was first used to determine if the results from such an analysis, with emphasis on the shear deformations between the belts, could be used to predict a relative ranking for belt edge endurance. It is shown that such an analysis can lead to erroneous conclusions. A three-dimensional analysis in which tires are modeled under free rotation and static vertical loading was performed next. This approach resulted in an improvement in the quality of the correlations. The differences in the predicted values of various stress analysis parameters for the three belt edge configurations are studied and their implication on predicting belt edge endurance is discussed.


Author(s):  
Radhika Theagarajan ◽  
Shubham Nimbkar ◽  
Jeyan Arthur Moses ◽  
Chinnaswamy Anandharamakrishnan

2011 ◽  
Vol 121-126 ◽  
pp. 1744-1748
Author(s):  
Xiang Yang Jin ◽  
Tie Feng Zhang ◽  
Li Li Zhao ◽  
He Teng Wang ◽  
Xiang Yi Guan

To determine the efficiency, load-bearing capacity and fatigue life of beveloid gears with intersecting axes, we design a mechanical gear test bed with closed power flow. To test the quality of its structure and predict its overall performance, we establish a three-dimensional solid model for various components based on the design parameters and adopt the technology of virtual prototyping simulation to conduct kinematics simulation on it. Then observe and verify the interactive kinematic situation of each component. Moreover, the finite element method is also utilized to carry out structural mechanics and dynamics analysis on some key components. The results indicate that the test bed can achieve the desired functionality, and the static and dynamic performance of some key components can also satisfy us.


2011 ◽  
Vol 148-149 ◽  
pp. 54-57
Author(s):  
Xiao Ping Lin ◽  
Yun Dong ◽  
Lian Wei Yang

The Al2O3 nano-films of different thicknesses (1~100nm) were successfully deposited on the monocrystalline Si surface by using ion beam sputtering deposition. The surface topography and the component of nano-films with different thickness were analyzed. The quality of the surface of nano-films was systematically studied. When the films’ thickness increase, the studies by atomic force microscope (AFM), X-ray photoelectron spectrum(XPS) show that the gathering grain continually grows up and transits from acerose cellula by two-dimensional growth to globularity by three-dimensional growth. The elements O, Al and Si were found on the surface of Al2O3 nano-films. With the thickness of the films increasing, the content of Al gradually increases and the intensity peak of Si wears off, the surface quality of the deposited films is ceaselessly improved


2010 ◽  
Vol 154-155 ◽  
pp. 1481-1484 ◽  
Author(s):  
Jun Zhong Guo ◽  
Jun Ping Yang

The on-off pressure mechanism has an important function to the printing press, the quality of which concerns the working performance of the printing machine and the quality of printed products directly. In this paper, the pneumatic on-off pressure mechanism is discussed; the work demand of order on-off pressure is analyzed. In addition, the three-dimensional digital model and the kinematic analysis process can be achieved on the basis of ADAMS software. What’s more, the on pressure value in the process of on pressure is derived from the kinematic analysis. Lastly, the relation between the motion of on-off pressure mechanism and cylinder’s angular displacement is analyzed, an important basis to the on-off pressure mechanism’s optimal design will be provided.


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