Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Ph. Kerremans ◽  
E. Gencheva ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Mediterranean Fruit Fly ◽  
Structural Basis ◽  
Mitotic Chromosomes ◽  
Genetic Sexing ◽  
Radiation Induced ◽  
Low Levels ◽  
The Mediterranean

Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.

Fluorescence banding in mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

Genome ◽  
1989 ◽  
Vol 32 (4) ◽  
pp. 580-588 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
X Chromosome ◽  
Sex Chromosomes ◽  
Silver Staining ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Mitotic Chromosomes ◽  
C Banding ◽  
Meiotic Chromosomes ◽  
The Mediterranean

Mitotic and meiotic chromosomes of the Mediterranean fruit fly, Ceratitis capitata, were studied using three counterstain-enhanced fluorescence staining methods. The tristaining technique allowed chromomycin A3 (CMA) and distamycin – diamidinophenylindole (DA–DAPI) fluorescence to be observed on the same chromosomes. DAPI–actinomycin D (DAPI–AMD) fluorescence was also carried out. These techniques were complemented with quinacrine staining and C-banding. The results were compared with earlier data on silver staining. The sex chromosomes, particularly the X chromosome, show great banding detail with extensive longitudinal differentiation in mitotic chromosomes. GC- and AT-specific fluorescence is not found in the expected reciprocal pattern at all sites. Comparison with C-banding and silver staining shows that intense fluorescence occurs in lightly C banded regions and silver bands correspond to fluorescent bands rather than nucleolar organizers. The combination of staining data suggests that much of the X chromosome has characteristics intermediate between heterochromatin and euchromatin. Meiotic X chromosomes show much less detail and reduced fluorescence intensity but can still be easily traced throughout meiosis and spermatogenesis.Key words: fluorescence banding, sex chromosomes, Mediterranean fruit fly, Ceratitis capitata.

scholarly journals Cryopreservation of Embryos of the Mediterranean Fruit Fly Ceratitis capitata Vienna 8 Genetic Sexing Strain

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160232 ◽  
Author(s):  
Antonios A. Augustinos ◽  
Arun Rajamohan ◽  
Georgios A. Kyritsis ◽  
Antigone Zacharopoulou ◽  
Ihsan ul Haq ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Genetic Sexing ◽  
The Mediterranean ◽  
Genetic Sexing Strain
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