Genetic stability of the D1Z2 region: implications for DNA genotyping and paternity testing

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 733-738 ◽  
Author(s):  
Katherine M. Tynan ◽  
Malcolm I. Parslow ◽  
David I. Hoar
Keyword(s):  
Ethnic Groups ◽  
Tandem Repeat ◽  
Genetic Stability ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Paternity Testing ◽  
Island Population ◽  
Excellent Candidate ◽  
Restriction Fragment Length Polymorphic ◽  
Dna Genotyping

The aim of the present study was to examine a single locus variable number tandem repeat for the purpose of DNA genotyping ("fingerprinting"). DNAs of 175 individuals from five ethnic groups (Black, Chinese, Japanese, Caucasian, and Melanesian) were analyzed. Restriction fragment length polymorphic analysis of random individuals revealed individual specific DNA patterns in all but one group. Among 20 Melanesian inhabitants of the Vanuatu islands in the southwest Pacific, three individuals were found to share a common pattern. This island population represents a "genetic isolate" and illustrates the importance of carrying out population studies on individual ethnic groups of interest. The complexity and the genetic stability of the D1Z2 region as revealed by the probe hMF1 make it an excellent candidate for DNA genotyping in paternity testing as 101 Caucasian individuals each had unique patterns for PstI and SinI digests.Key words: DNA fingerprinting, variable number tandem repeat, paternity testing, DNA genotyping.

Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis

Vaccine ◽  
2007 ◽  
Vol 25 (15) ◽  
pp. 2858-2862 ◽  
Author(s):  
David García-Yoldi ◽  
Philippe Le Fleche ◽  
Clara M. Marín ◽  
María J. De Miguel ◽  
Pilar M. Muñoz ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Tandem Repeat ◽  
Genetic Stability ◽  
Brucella Melitensis ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

2014 ◽  
Vol 14 (1) ◽  
pp. 186 ◽  
Author(s):  
Elaine Maria Dorneles ◽  
Jordana Santana ◽  
Telma Alves ◽  
Rebeca Pauletti ◽  
Juliana Pinto da Silva Mol ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Brucella Abortus ◽  
Genetic Stability ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

Genetic stability of Brucella abortus S19 and RB51 vaccine strains by multiple locus variable number tandem repeat analysis (MLVA16)

Vaccine ◽  
2013 ◽  
Vol 31 (42) ◽  
pp. 4856-4859 ◽  
Author(s):  
Elaine Maria Seles Dorneles ◽  
Ana Paula Paiva de Faria ◽  
Rebeca Barbosa Pauletti ◽  
Jordana Almeida Santana ◽  
George Afonso Vítor Caldeira ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Brucella Abortus ◽  
Genetic Stability ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Multiple-Locus Variable-Number Tandem Repeat Analysis of Dutch Bordetella pertussis Strains Reveals Rapid Genetic Changes with Clonal Expansion during the Late 1990s

2004 ◽  
Vol 186 (16) ◽  
pp. 5496-5505 ◽  
Author(s):  
Leo M. Schouls ◽  
Han G. J. van der Heide ◽  
Luc Vauterin ◽  
Paul Vauterin ◽  
Frits R. Mooi
Keyword(s):  
The Netherlands ◽  
Tandem Repeat ◽  
Bordetella Pertussis ◽  
Minimum Spanning Tree ◽  
Whooping Cough ◽  
Variable Number Tandem Repeat ◽  
Clonal Expansion ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

ABSTRACT Bordetella pertussis, the causative agent of whooping cough, has remained endemic in The Netherlands despite extensive nationwide vaccination since 1953. In the 1990s, several epidemic periods have resulted in many cases of pertussis. We have proposed that strain variation has played a major role in the upsurges of this disease in The Netherlands. Therefore, molecular characterization of strains is important in identifying the causes of pertussis epidemiology. For this reason, we have developed a multiple-locus variable-number tandem repeat analysis (MLVA) typing system for B. pertussis. By combining the MLVA profile with the allelic profile based on multiple-antigen sequence typing, we were able to further differentiate strains. The relationships between the various genotypes were visualized by constructing a minimum spanning tree. MLVA of Dutch strains of B. pertussis revealed that the genotypes of the strains isolated in the prevaccination period were diverse and clearly distinct from the strains isolated in the 1990s. Furthermore, there was a decrease in diversity in the strains from the late 1990s, with a remarkable clonal expansion that coincided with the epidemic periods. Using this genotyping, we have been able to show that B. pertussis is much more dynamic than expected.

Sign in / Sign up

Export Citation Format

Share Document