A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 543-552 ◽  
Author(s):  
Benoit S. Landry ◽  
Nathalie Hubert ◽  
Takeomi Etoh ◽  
John J. Harada ◽  
Stephen E. Lincoln
Keyword(s):  
Brassica Napus ◽  
Genetic Map ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rflp Markers ◽  
Cdna Clones ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

F2 segregation analyses of DNA restriction fragment length polymorphisms (RFLPs) detected between two cultivars of canola ('Westar' × Topas') was used to construct a detailed genetic map of Brassica napus. RFLP markers were from a seedling-specific cDNA library. They were either randomly selected or previously characterized as seedling-specific cDNA clones. The 120 loci so far mapped in B. napus covered 1413 recombination units. They are assembled into 19 linkage groups. Seventeen loci were found unlinked to any other markers. Few polymorphisms were detected with the seedling-specific cDNAs and only seven loci could be mapped in this cross. Duplication of RFLP loci was extensive and reflects the amphidiploid nature of this species. However, several rearrangements of the linear order of duplicated loci could be seen. This RFLP study in B. napus provides important information on genome organization of functional DNA sequences and complements our current RFLP mapping effort in Brassica oleracea. The genetic markers of this map are currently being used in several breeding applications, such as tagging important agronomic traits and fingerprinting breeding lines and cultivars of canola, a major oilseed crop.Key words: genetic mapping, Brassica napus, breeding, restriction fragment length polymorphisms.

The identification of restriction fragment length polymorphisms linked to seed colour genes in Brassica napus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 534-542 ◽  
Author(s):  
A. E. Van Deynze ◽  
B. S. Landry ◽  
K. P. Pauls
Keyword(s):  
Brassica Napus ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Rflp Markers ◽  
Seed Colour ◽  
Dh Population ◽  
Length Polymorphisms ◽  
Dh Lines ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) linked to genes controlling seed colour were identified in rapeseed (Brassica napus). The efficiency of the RFLP analysis was enhanced by utilizing bulked segregant analysis, DNA clones that had previously been used to construct a RFLP map of B. napus, and a doubled-haploid (DH) population segregating for seed colour. Markers for two of the three seed colour genes segregating in the DH population were identified on the basis of χ2 analyses of marker distributions among visually classified black-, brown-, and yellow-seeded DH lines as well as ANOVA and quantitative trait locus analysis of light-reflectance measurements from seeds of the DH lines. The RFLP markers linked to seed colour that were identified in the present study will allow breeding strategies based on genotype selection to be developed for seed colour in rapeseed.Key words: RFLP markers, seed colour genes, rapeseed.

scholarly journals Sequences of Probes Revealing Mapped Restriction Fragment-length Polymorphisms in Cucumber

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 323-324
Author(s):  
Michael J. Havey
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Expressed Sequence Tags ◽  
Restriction Fragment Length Polymorphisms ◽  
Cdna Clones ◽  
Chromosome 4 ◽  
Sequence Comparisons ◽  
Length Polymorphisms ◽  
Expressed Sequence ◽  
Restriction Fragment Length

PstI-genomic and cDNA clones revealing mapped restriction fragment-length polymorphisms (RFLP) in cucumber (Cucumis sativus L.) were sequenced in order to ensure that these clones remain available and to determine if any clones showing genetic linkage in cucumber are physically linked in Arabidopsis thaliana. Sequence comparisons using translated searches revealed that 80% of the cucumber cDNA clones showed significant (≤e-20) similarities to Arabidopsis expressed sequence tags (ESTs) or genomic sequences, as opposed to relatively few (32%) of the cucumber genomic clones. Two clones revealing RFLPs linked at 2 cM in cucumber showed significant (≤e-20) similarities to sequences separated by 347,616 basepairs on chromosome 4 of Arabidopsis.

Polymorphisms in the nuclear DNA of Achlya species: some taxonomic implications

1989 ◽  
Vol 35 (12) ◽  
pp. 1146-1155 ◽  
Author(s):  
J. Stephen Horton ◽  
Paul A. Horgen
Keyword(s):  
Southern Blot ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Dna Polymorphisms ◽  
Rrna Gene ◽  
Cdna Clones ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Within the genus Achyla, which belongs to the class of fungi known as the Oomycetes, taxonomic judgments have traditionally been made using a variety of sexual criteria. We have used restriction fragment length polymorphisms as a new taxonomic character to examine intra- and inter-specific variation within this genus. Using a cDNA clone coding for the Achlya 18S rRNA gene as a hybridization probe, a 10-kb fragment of ribosomal DNA (rDNA) from Achlya ambisexualis strain E87 was cloned and then mapped for selected restriction enzyme sites. In Southern blot hybridizations, both this rDNA fragment and cloned 18S cDNAs revealed differences in the rDNA organization of A. ambisexualis E87 (male) and a female isolate of A. ambisexualis strain 734. No differences in the rDNAs were detected between the two heterothallic isolates A. ambisexualis E87 and A. bisexualis 65-1. Southern blot hybridizations suggested that two different rDNA organizations may exist within the genome of the homothallic strain A. heterosexualis B14. cDNA clones coding for two different hormonally regulated genes revealed the same relationships between the four isolates studied as those determined with rDNA probes. Two homothallic Achlya strains recently isolated from nature were found to have additional DNA polymorphisms not detected in the laboratory strains. Phenetic analysis distinguished the same similarities that were evident upon inspection of the hybridization data. Taken together, these data suggest different relationships between the isolates examined than do the previous taxonomic criteria by which species have been delimited within this genus.Key words: Achlya; restriction fragment length polymorphisms; ribosomal DNA; taxonomy.

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