Use of double-ditelosomic and normal chromosome 1D recombinant substitution lines to map Sr33 on chromosome arm 1DS in wheat

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 505-508 ◽  
Author(s):  
S. S. Jones ◽  
J. Dvořák ◽  
D. R. Knott ◽  
C. O. Qualset
Keyword(s):  
Triticum Aestivum ◽  
Stem Rust ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Stem Rust Resistance ◽  
Substitution Lines ◽  
Triticum Tauschii ◽  
Chromosome Arm

Chromosome 1D homozygous recombinant substitution lines derived from Triticum aestivum 'Chinese Spring' (cross 1) or 'Chinese Spring' double-ditelosomic 1D (cross 2) hybridized with a disomic substitution line of Triticum tauschii chromosome 1D in 'Chinese Spring' were used to investigate the linkage relationships among Glu-D1, encoding subunits of high molecular weight glutenin storage proteins; Gli-D1, encoding gliadin storage proteins; Sr33, conferring stem rust resistance; and the centromere. Based on analysis of 88 and 91 recombinant substitution lines of crosses 1 and 2, respectively, Sr33 is tightly linked to Gli-D1 on chromosome arm 1DS (5.6 and 7.6% recombination) and less tightly to the centromere (29.6%, cross 2) and to Glu-D1 (40.9 and 39.5%). The order of the loci is Glu-D1 – centromere – Sr33 – Gli-D1.Key words: stem rust resistance, seed storage protein, glutenin, gliadin, Triticum aestivum, Triticum tauschii.

Linkage relations of Gli-D1, Rg2, and Lr21 on the short arm of chromosome 1D in wheat

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 937-940 ◽  
Author(s):  
S. S. Jones ◽  
J. Dvořák ◽  
C. O. Qualset
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Storage Protein ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Leaf Rust Resistance ◽  
Triticum Tauschii ◽  
Chromosome Arm

Homozygous recombinant substitution lines, derived from a cross of Triticum aestivum 'Chinese Spring' with a disomic substitution line of Triticum tauschii chromosome 1D in 'Chinese Spring', were used to investigate the linkage relationships among the loci Glu-D1, encoding high molecular weight glutenin storage proteins, Gli-D1, encoding gliadin storage proteins, Rg2, controlling glume color, and Lr21, conferring leaf-rust resistance. Gli-D1, on chromosome arm 1DS, is tightly linked to Rg2 and Lr21 (1.4 and 5.6% recombination, respectively). The order of the loci is Gli-D1–Rg2–Lr21. Glu-D1, on chromosome arm 1DL, segregates independently (P = 0.43) of Gli-D1, Rg2, and Lr21. The position of Glu-D1, Gli-D1, Rg2, and Lr21 in the genetic linkage map of chromosome 1D agrees with the position of storage protein, glume color, and rust-resistance loci on chromosomes 1A and 1B.Key words: leaf-rust resistance, seed storage protein, glutenin, gliadin, glume color, Triticum aestivum, Triticum tauschii.

Chromosomal location of seed storage protein genes in the genome of Elytrigia elongata

1986 ◽  
Vol 28 (5) ◽  
pp. 818-830 ◽  
Author(s):  
J. Dvořák ◽  
D. D. Kasarda ◽  
M. D. Dietler ◽  
E.-J. L. Lew ◽  
O. D. Anderson ◽  
...  
Keyword(s):  
Chinese Spring ◽  
Storage Proteins ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Glutenin Subunit ◽  
Gene Location ◽  
Substitution Lines ◽  
Hmw Glutenin Subunit ◽  
Chromosome Arm ◽  
Hmw Glutenin

Additions of complete and telocentric chromosomes of Elytrigia elongata (Host) Nevski in Triticum aestivum L. 'Chinese Spring' were employed to assign the genes coding for seed storage proteins to chromosome arms in the E. elongata genome. Genes coding for prolamins equivalent to wheat gliadins were found on chromosome arms 1ES and 6Ep. Genes on chromosome arm 1ES, which is presumably the p arm, coded for several components with electrophoretic mobilities (lactate–PAGE) corresponding to those of β-, γ-, and ω- gliadins and those on chromosome arm 6Ep coded for two components with mobilities corresponding to those of β-gliadins. Amino acid sequencing of mixtures of prolamins from E. elongata and from E. pontica (Podp.) Holub, a species closely related to E. elongata, indicated that prolamins of these species correspond to α-type (which includes β-gliadins), γ-type, and ω-type gliadins. Restriction fragments of genomic DNAs from substitution lines of chromosome 6E of E. elongata in 'Chinese Spring' were separated electrophoretically in agarose gels and probed with a cloned α-type gliadin gene from 'Yamhill'. This Southern blot showed that chromosome 6E yields DNA fragments identical in size to those characteristic of the α-gliadin gene cluster that is on chromosome 6A of 'Chinese Spring', 'Cheyenne', and 'Yamhill'. These results indicate that structural genes for prolamins of Elytrigia are similar to those of wheat gliadins and are located on the same chromosome arms as those in Triticum species. A high molecular weight (HMW) protein likely to be a HMW glutenin subunit was located on the long arm of chromosome 1E, which presumably is the q arm; this also is in accordance with the location of HMW glutenin subunit genes in Triticum. It is concluded that the appearance of α-type gliadin genes on chromosomes of homoeologous group 6 in T. aestivum occurred prior to divergence of Triticum and Elytrigia but after the divergence of Secale, Hordeum, and the Triticum–Elytrigia lineages, since neither Secale or Hordeum appear to have α-type genes. It is, however, possible that α-type gliadin genes were deleted from the ancestors of Secale and Hordeum after divergence from the Triticum–Elytrigia lineage.Key words: Elytrigia elongata, gene location, prolamins, gliadins, wheat.

The field reaction to stem rust of 'Chinese Spring' substitution lines carrying chromosomes from 'Hope' and 'Thatcher' wheats

1986 ◽  
Vol 28 (1) ◽  
pp. 12-16
Author(s):  
D. R. Knott
Keyword(s):  
Stem Rust ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Triticum Aestivum L ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Substitution Lines ◽  
Field Reaction ◽  
Rust Severity

Studies were done in an attempt to determine the inheritance of adult plant resistance to stem rust (Puccinia graminis f. sp. tritici Eriks. and E. Henn.) in the wheat (Triticum aestivum L.) cultivars 'Hope' and 'Thatcher'. 'Chinese Spring' substitution lines carrying individual chromosomes from 'Hope' and 'Thatcher' were tested in field rust nurseries, three times each with races 15B-1 and 56, and twice with multirace mixtures. In 1976 it was found that the date of heading often had a significant effect on rust severity, with early lines showing less rust. In 1977 and 1984 the lines were divided into three groups based on maturity and were planted on three dates about 10 days apart to make heading dates more uniform. The data indicate that 'Hope' has genes for resistance to race 56 on chromosomes 3B (Sr2) and 4D, and to a multirace mixture on 1B, 3B, and 7B. 'Thatcher' possibly has genes for resistance to race 56 on chromosomes 6A and 3B (Sr12). The results show that the resistance of both cultivars is complex and most genes have only small effects.Key words: Triticum, Puccinia, rust resistance, substitution lines.

GENETICS OF STEM RUST RESISTANCE IN WHEAT CULTIVARS ROMANY, Es.P 518/9, BONNY AND TAMA

1975 ◽  
Vol 17 (4) ◽  
pp. 667-674 ◽  
Author(s):  
P. L. Dyck ◽  
G. J. Green
Keyword(s):  
Triticum Aestivum ◽  
Stem Rust ◽  
Rust Resistance ◽  
Triticum Aestivum L ◽  
Stem Rust Resistance ◽  
Puccinia Graminis ◽  
Wheat Cultivars ◽  
Genetics Of Resistance ◽  
Seedling Resistance ◽  
Moderate Resistance

The genetics of resistance to stem rust (Puccinia graminis tritici) was investigated in wheat (Triticum aestivum L.) cultivars Romany, Es.P 518/9, Bonny and Tama that are resistant to many races in both Canada and Kenya. Seedling resistance in the four cultivars to 12 Canadian races is controlled primarily by previously identified genes. The results indicate that the cultivars have the following genes: Romany — Sr5, Sr6, Sr7a, Sr9b and SrW; Es.P 518/9 — Sr5, Sr6, Sr7a, Sr8, Sr9b, SrW and possibly Sr17; Bonny — Sr6 and Sr11; and Tama — Sr6 and Sr8. Gene SrW confers moderate resistance and is also present in the cultivar Webster.

SEEDLING REACTIONS TO STEM RUST OF LINES OF MARQUIS WHEAT WITH SUBSTITUTED GENES FOR RUST RESISTANCE

1960 ◽  
Vol 40 (3) ◽  
pp. 524-538 ◽  
Author(s):  
G. J. Green ◽  
D. R. Knott ◽  
I. A. Watson ◽  
A. T. Pugsley
Keyword(s):  
High Temperature ◽  
North American ◽  
Stem Rust ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Wheat Variety ◽  
Substitution Lines ◽  
Moderate Resistance

Lines of the wheat variety Marquis carrying genes Sr6, Sr7, Sr8, Sr9, Sr10, and Sr6 plus Sr7 for resistance to stem rust were produced by the backcross method. The reactions of these lines to 99 North American cultures of 29 races of stem rust and to 8 Australian cultures were determined. Genes Sr6, Sr8, and Sr9 conferred a uniform type of resistance to most of the cultures. Genes Sr7 and Sr10 conferred only moderate resistance to a few cultures. Genes Sr6, Sr8, and Sr9 appeared to confer the same kind of resistance in Marquis after 5 backcrosses as in the source varieties, but Sr7 and Sr10 seemed less effective. Results of the infection studies indicated that Sr7 may be allelic with a gene for resistance in Marquis and that at least two alleles conditioning different rust reactions may occur at the Sr9 locus. The reactions of the lines with Sr6, Sr8, and Sr9 were nearly identical with those of the Chinese Spring substitution lines, Red Egyptian XX, Red Egyptian VI, and Red Egyptian XIII respectively, which presumably have these genes. The data indicated that Sr6 is the same as SrKa1 and Sr9 is the same as SrKb1. The resistance of lines with Sr7, Sr8, and Sr9 was affected only slightly by temperature but the resistance of lines with Sr10 and especially of those with Sr6 diminished at high temperature.

Inheritance of leaf rust and stem rust resistance in 'Roblin' wheat

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 289-293 ◽  
Author(s):  
P. L. Dyck
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Stem Rust Resistance ◽  
Puccinia Graminis ◽  
Susceptible Cultivar ◽  
Wheat Leaf

The Canadian common wheat (Triticum aestivum L.) cultivar 'Roblin' is resistant to both leaf rust (Puccinia recondita Rob. ex. Desm.) and stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. and E. Henn.). To study the genetics of this resistance, 'Roblin' was crossed with 'Thatcher', a leaf rust susceptible cultivar, and RL6071, a stem rust susceptible line. A set of F6 random lines was developed from each cross. The random lines and the parents were grown in a field rust nursery artificially inoculated with a mixture of P. recondita and P. graminis isolates and scored for rust reaction. The same material was tested with specific races of leaf rust and stem rust. These data indicated that 'Roblin' has Lr1, Lr10, Lr13, and Lr34 for resistance to P. recondita and Sr5, Sr9b, Sr11, and possibly Sr7a and Sr12 for resistance to P. graminis. In a 'Thatcher' background, the presence of Lr34 contributes to improve stem rust resistance, which appears also to occur in 'Roblin'.Key words: Triticum aestivum, wheat, leaf rust resistance, stem rust resistance.

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