Use of Ipomoea trifida (HBK.) G. Don germ plasm for sweet-potato improvement. 2. Fertility of synthetic hexaploids and triploids with 2n gametes of I. trifida, and their interspecific crossability with sweet potato

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 209-214 ◽  
Author(s):  
Rosanna Freyre ◽  
Masaru Iwanaga ◽  
Gisella Orjeda
Keyword(s):  
Sweet Potato ◽  
Egg Production ◽  
Ipomoea Batatas ◽  
2N Gametes ◽  
Pollen Production ◽  
2N Pollen ◽  
Ipomoea Trifida ◽  
Potato Improvement ◽  
Synthetic Hexaploids ◽  
Interspecific Crossability

Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida were used in crosses to estimate their male and female fertility and their crossability with sweet potato, Ipomoea batatas (L.) Lam. Several important conclusions were drawn. (i) The fertility of pollen of the triploid plants was confirmed, which suggested they are truly functional 2n pollen. (ii) The average male fertility of hexaploid plants was higher than that of triploid with 2n pollen production plants. (iii) 2n egg production was observed in several triploid genotypes, (iv) From a polycross, 3079 seeds with normal shapes were produced. These represent a population of hexaploid I. trifida with a wide genetic base, and they may be important material for sweet-potato improvement, (v) A total of 3275 seeds were obtained from crosses with six sweet-potato female parents, of which only 60 germinated, indicating the existence of an interspecific barrier. The implications of 2n gametes for breeding and evolution of sweet potato are also discussed.Key words: Ipomoea batatas, Ipomoea trifida, 2n gametes, sexual polyploidization, triploid.

Use of Ipomoea trifida (HBK.) G. Don germ plasm for sweet potato improvement. 1. Development of synthetic hexaploids of I. trifida by ploidy-level manipulations

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Masaru Iwanaga ◽  
Rosanna Freyre ◽  
Gisella Orjeda
Keyword(s):  
Sweet Potato ◽  
Ploidy Level ◽  
Ipomoea Batatas ◽  
Colchicine Treatment ◽  
Pollen Production ◽  
2N Pollen ◽  
Ipomoea Trifida ◽  
Pollen Sample ◽  
Colchicine Solution ◽  
Synthetic Hexaploids

Crosses were made between 21 tetraploid accessions and 41 diploid accessions of Ipomoea trifida, obtaining a total of 9185 triploid seeds from 215 different cross combinations. Doubling the somatic chromosome number of the triploids was attempted by colchicine treatment on young seedlings to obtain synthetic hexaploids. A total of 787 axillary buds of 316 triploid plants were treated with a 0.5% colchicine solution for 24 h, applied to cotton plugs surrounding the buds. The survival rate of the treated buds was, on average, 41%. The ploidy level in germ-layer L2 was determined in 258 clones by evaluating a pollen sample from each clone. Fifty-five clones were selected for high stainability and pollen size. The selected genotypes were meiotically analyzed. Twenty-two of them were identified as hexaploid and 33 as triploid with 2n pollen production. The present study is the first report on 2n pollen production in triploid plants of Ipomoea species. The use of these triploid clones with 2n pollen production and synthetic hexaploid clones for sweet-potato breeding is discussed.Key words: Ipomoea batatas, Ipomoea trifida, colchicine, triploids, 2n pollen.

scholarly journals PROTOPLAST ISOLATION AND CULTURE OF SOME IPOMOEA SPECIES IN THE SECTION BATATAS.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1155f-1155
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
World Wide ◽  
Protoplast Isolation ◽  
Cellular Level ◽  
Ipomoea Trifida ◽  
Centers Of Origin ◽  
Tuber Production ◽  
Ipomoea Triloba ◽  
Ipomoea Species

Sweet potato (Ipomoea batatas L. Lam) ranks second in world wide root and tuber production. Loss of genetic resources in the primary centers of origin have been occurring for a number of years. Utilization of the genetic diversity of wild types and related species cart expand breeding potential and efficiency. Two species, Ipomoea trifida and Ipomoea triloba, have been identified as possible progenitor species or sweet potato. Plant improvement at the cellular level using protoplasts is a novel alternative to conventional breeding practices. Protoplast plating efficiency appears to be genotype dependent. Roots have been regenerated from Ipomoea trifida protoplasm calli on Murashige and Skoog's medium containing zeatin or kinetin.

Bilateral sexual tetraploidization in red clover

1985 ◽  
Vol 27 (1) ◽  
pp. 64-68 ◽  
Author(s):  
W. A. Parrott ◽  
R. R. Smith ◽  
M. M. Smith
Keyword(s):  
Egg Production ◽  
Trifolium Pratense ◽  
2N Gametes ◽  
Red Clover ◽  
Female Plant ◽  
2N Pollen ◽  
Sexual Polyploidization ◽  
2N Eggs ◽  
Trifolium Pratense L ◽  
Bilateral Sexual Polyploidization

Twenty-four diploid red clover (Trifolium pratense L.) plants, from the cultivars 'Arlington', 'Florex', and 'Redman', and C760, a Wisconsin synthetic, were tested for 2n egg production by crossing them with tetraploid plants. Four plants were identified and selected as 2n egg producers. These plants were then crossed with a known producer of 2n pollen in an attempt to obtain tetraploid plants through bilateral sexual polyploidization. Thirteen percent of the seed obtained in one of the 2x–2x crosses were tetraploid. The female plant produced an estimated 0.14 2n eggs per 1000 flowers in the cross. In a second experiment, bilateral sexual polyploidization was attempted, using 14 plants which had not been previously tested for 2n egg production. Two of these plants produced one and two tetraploids each, representing 2n egg frequencies of 3.33 and 5.00 2n eggs per 1000 flowers, respectively. It seems likely that it will be possible to use bilateral sexual polyploidization to tetraploidize red clover germplasm in the future.Key words: tetraploid red clover, Trifolium pratense. 2n gametes.

scholarly journals KAJIAN PEMANFAATAN SEKAM PADI DIFERMENTASI Aspergillus niger DALAM RANSUM DISUPLEMENTASI TEPUNG DAUN UBI JALAR UNGU (Ipomoea batatas, L.) TERHADAP PENAMPILAN ITIK BALI UMUR 24 -36 MINGGU

2018 ◽  
Vol 21 (2) ◽  
pp. 45
Author(s):  
SUSILA T. G. O. ◽  
T. G. B. YADNYA ◽  
A. A. A. S. TRISNADEWI
Keyword(s):  
Sweet Potato ◽  
Egg Production ◽  
Ipomoea Batatas ◽  
Feed Consumption ◽  
Rice Hull ◽  
Potato Leaf ◽  
Leaf Meal ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Purple Sweet Potato

The experiment was carried out to study the effect on fermented rice hull in diets supplemented with purplesweet potato (Ipomoea batatas L.) leaves meal on performance of bali ducks. A completely randomized design withfive treatments such as diet without rice hull (A), diet contaning 10% rice hull (B), diet containing 10% fermentedrice hull (C), ration contaning 9% rice hull and 1% purple sweet potato leaf meal (D), diet containing 9% fermentedrice hull and 1% purple sweet potato leaf meal (E). Each treatment in three replications consists of 5 female ducks.The variables observed were feed consumption, antioxidant capacity, total egg weight and FCR, and total eggs, eggproduction per day, and means of eggs weight. It showed that diet feed consumption of ducks fed E treatment found3.32% lower (P<0,05), total eggs 47,56% higher (P<0,05) compared to A. In addition, E treatment can increasetotal amount of eggs, egg production per day, and 29,16%, 29,15% dan 12,12% means of eggs (P<0,05) compared toA treatment. It can be concluded that ducks fed diets containing fermented rice hull and purple sweet potato leafmeal supplemented can improve efficiency of diets and production of eggs.

2n GAMETES IN DIPLOID SOLANUM: FREQUENCY AND TYPES OF SPINDLE ABNORMALITIES

1982 ◽  
Vol 24 (3) ◽  
pp. 301-314 ◽  
Author(s):  
R. E. Veilleux ◽  
N. A. McHale ◽  
F. I. Lauer
Keyword(s):  
Cross Sections ◽  
Meiotic Division ◽  
2N Gametes ◽  
Anatomical Study ◽  
Solanum Phureja ◽  
Spindle Orientation ◽  
Pollen Production ◽  
2N Pollen ◽  
Genetic Theory ◽  
Parallel Spindles

Cytological disturbances resulting in 2n pollen were examined in four clones of diploid (2x = 24) Solanum phureja Juz. and Buk.; two (148-17 and 127-14) were variable and two (154-1 and B2-11) were consistently high in 2n pollen production. Three types of abnormal spindle orientation were observed at the second meiotic division: fused, tripolar, and parallel spindles (fs, tps, and ps, respectively). All four genotypes had fs. Three had tps, and ps was least frequent, occuring in two genotypes only. Cross-sections of buds revealed that all three abnormalities can occur in adjacent cells of a locule. In all genotypes, fs predominated at high levels of expression of 2n gametes. At lower levels of expression (i.e. less than 60%, which occurred only in 148-17 and 127-14), however, the frequencies of tps and ps increased. In general, there was little evidence for localization of dyads (resulting from fs or ps) or tetrads (resulting from normal divisions) within cross sections of locules, but 52.4% of all tetrads which occurred in a single locule of genotype 127-14 were found in 25% of the locular area. Cross-sections of buds revealed variation in frequency of 2n gametes among anthers (61.7 vs. 5.6% in 148-17) and between locules of an anther (73.1 vs. 90.0% in B2-11). Implications of the anatomical study were applied to current genetic theory regarding the development of 2n gametes in potato species.

scholarly journals DIVERSITAS GENETIK KERABAT LIAR UBI JALAR IPOMOEA TRIFIDA (H.B.K.) G.DON. BERUMBI ASAL CITATAH JAWA BARAT BERDASARKAN KARAKTER KROMOSOM (Genetic Diversity Of Wild Relative Of Sweet Potato Tubered-Ipomoea Trifida (H.B.K.) G.don. Originated From Citatah West Java Based On Chromosome Characters)

2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Tia Setiawati
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Principal Component ◽  
Component Analysis ◽  
West Java ◽  
Ipomoea Trifida ◽  
Plant Materials ◽  
Materials Used

AbstrakKerabat liar ubi jalar Ipomoea trifida asal Citatah Jawa Barat potensial digunakan dalam program pemuliaan tanaman ubi jalar (Ipomoea batatas (L.) Lam.) Untuk mengetahui diversitas genetik I. trifida  dilakukan pengamatan terhadap karakter kromosom. Bahan tanaman yang digunakan  adalah 10 spesies I. trifida berumbi. Hubungan kekerabatan antar spesies diketahui melalui analisis klaster dan Analisis Komponen Utama/Principal Component Analysis (PCA). Hasil pengamatan menunjukkan pengujian pada 10 aksesi I. trifida berumbi dengan menggunakan 9 karakter kromosom menghasilkan jarak ketidakmiripan (Euclidean coeffisien) yang berkisar 1,75 – 6,22 dan menunjukkan diversitas yang luas. Dendogram yang dihasilkan pada jarak ketidakmiripan 5,23 menunjukkan terbentuknya 3 klaster utama. Analisis Komponen Utama (PCA) menghasilkan 2 komponen utama pertama (PC1 dan PC2) yang telah dapat menjelaskan 89,64% dari total variasi. Kata kunci : Ipomoea trifida, Analisis Klaster, Analisis Komponen UtamaAbstractWild relatives of sweet potato, Ipomoea trifida originated from Citatah West Java was potential use in plant breeding programs of Ipomoea batatas (L.) Lam. To determine the genetic diversity of I. trifida, observation on characters of chromosomes of I.trifida was conducted. Plant materials used ten accessions  of tubered-I. trifida. Relationship between species identified by cluster analysis and Principal Component Analysis (PCA). The results showed that the observation on the 10 accession of tubered-I. trifida using 9 characters chromosome produces dissimilarities distance (Euclidean coefficient) ranging from 1.75 to 6.22. Dendogram generated  at a dissimilarity distance of 5.23 showed the formation of three main clusters. Principal Component Analysis (PCA) produced first two principal component (PC1 and PC2), which has been able to explain 89.64% of the total variation.Keyword  : Ipomoea trifida, Clustering Analysis, Principal Component Analysis

scholarly journals Genome-Wide Identification and Expression Analysis of JAZ Family Involved in Hormone and Abiotic Stress in Sweet Potato and Its Two Diploid Relatives

2021 ◽  
Vol 22 (18) ◽  
pp. 9786
Author(s):  
Zhengwei Huang ◽  
Zhen Wang ◽  
Xu Li ◽  
Shaozhen He ◽  
Qingchang Liu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Sweet Potato ◽  
Stress Responses ◽  
Growth And Development ◽  
Ipomoea Batatas ◽  
Interaction Network ◽  
Functional Study ◽  
Abiotic Stress Response ◽  
Ipomoea Trifida ◽  
Ipomoea Triloba

Jasmonate ZIM-domain (JAZ) proteins are key repressors of a jasmonic acid signaling pathway. They play essential roles in the regulation of plant growth and development, as well as environmental stress responses. However, this gene family has not been explored in sweet potato. In this study, we identified 14, 15, and 14 JAZs in cultivated hexaploid sweet potato (Ipomoea batatas, 2n = 6x = 90), and its two diploid relatives Ipomoea trifida (2n = 2x = 30) and Ipomoea triloba (2n = 2x = 30), respectively. These JAZs were divided into five subgroups according to their phylogenetic relationships with Arabidopsis. The protein physiological properties, chromosome localization, phylogenetic relationship, gene structure, promoter cis-elements, protein interaction network, and expression pattern of these 43 JAZs were systematically investigated. The results suggested that there was a differentiation between homologous JAZs, and each JAZ gene played different vital roles in growth and development, hormone crosstalk, and abiotic stress response between sweet potato and its two diploid relatives. Our work provided comprehensive comparison and understanding of the JAZ genes in sweet potato and its two diploid relatives, supplied a theoretical foundation for their functional study, and further facilitated the molecular breeding of sweet potato.

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