Polytene chromosome maps in the Medfly Ceratitis capitata

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 184-197 ◽  
Author(s):  
A. Zacharopoulou

Polytene chromosome maps of the five autosomes from salivary gland cells in Ceratitis capitata are presented, and the more characteristic features of each element are described. The correlation of the polytene elements to miotic chromosomes and linkage groups is established by using various Y-autosome and autosome-autosome translocation lines. Two loci, dp (black pupal case) and w (white pupal case), are mapped to the third and fifth chromosome, respectively. In addition to the polytene maps presented, some extra figures of specific chromosomal regions are given for easier identification of each polytene element.Key words: polytene chromosome maps, Ceratitis capitata.

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 598-611 ◽  
Author(s):  
D. G. Bedo

Polytene chromosome reference maps of the five autosomes of Ceratitis capitata from male pupal orbital bristle trichogen cells are presented and a correlation is established between two of them and the two largest of the five autosomes in the haploid mitotic complement. Characteristic features of each chromosome are described identifying areas that are difficult to analyze and noting the existence of common alternative band expression. A quantitative analysis of the mitotic karyotype of C. capitata indicates that the two smallest autosome pairs cannot be reliably distinguished. This may present problems with future attempts to establish homologies between the remaining mitotic and polytene chromosomes. A comparison of polytene chromosome banding patterns from salivary gland and trichogen cells failed to find any homologous regions, or even to identify homologous chromosomes. The banding differences are not explained by variation in puffing patterns, heterochromatin expression, or polyteny levels, but appear to reflect fundamental differences in banding patterns of the chromosomes in each tissue. Key words: Ceratitis capitata, polytene chromosome map, mitotic chromosome measurements.


Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 702-705 ◽  
Author(s):  
W. Staiber

A small supernumerary polytene chromosome was found during the last 8 years in some rare cases in larval salivary gland cells of Acricotopus lucidus (Diptera, Chironomidae). The chromosome may be derived from the germ line restricted parts of the genome. It consists of a short heterochromatic segment and of euchromatic sections with banding patterns homologous to sections of the short arm of soma chromosome I. When examining male meiosis, an exceptional small germ line limited chromosome was found. It is believed that this chromosome was not always recognized during soma elimination as a germ line limited chromosome, probably because of its partial homology to one of the soma chromosomes, and was then polytenized in salivary gland cells. Another germ line limited chromosome with a characteristic morphology and with a special behavior in differential gonial mitosis was found to have existed for more than 12 years in a laboratory stock. In differential gonial mitosis this special germ line limited chromosome partly pairs with the long arm of soma chromosome I. The present results strongly support the idea that the germ line limited chromosomes of A. lucidus are derived from the soma chromosomes, and show that chromosomes of the germ line restricted part of the genome can persist for many generations in a laboratory stock in spite of complex chromosome elimination mechanisms in the primary germ cells. Key words: germ line limited chromosomes, supernumerary polytene chromosome, salivary gland, Acricotopus lucidus.


1989 ◽  
Vol 67 (2) ◽  
pp. 421-429 ◽  
Author(s):  
George N. Thomopoulos ◽  
Eleftherios P. Neophytou ◽  
Costas D. Kastritsis

Early in the third-instar stage of Drosophila auraria, the salivary gland cells produce small secretory granules of low electron density which empty their flocculent contents into the lumen of the gland. At that stage, the Golgi complexes consist of vesiculated, round cisternae which, as middle third instar is approached, change to their classical appearance. Cytoplasmic protrusions, intramitochondrial granules, and close contacts of mitochondria with lipid droplets are observed during the early developmental stages. At about the middle of the third instar the glue secretory granules start being produced. These secretory granules contain a granular material (where small amounts of vicinal glycol and sulfated groups of complex carbohydrates have been detected) of medium electron density. The glue secretory granules can be classified according to their electron density into two populations. Before exocytosis large amounts of glycogen particles are observed, providing some of the energy needed during the final step of exocytosis. Two different types of ribonucleoprotein particles in puffing sites of the chromosomes are observed. Of these, the larger ones consist of a core surrounded by smaller particles; we suspect that they may be produced in the Balbiani rings observed in the polytene chromosomes of this species. Nuclear blebs and oval bodies are common, particularly during the late developmental stages. The functional significance of these findings during development is discussed.


2020 ◽  
Vol 133 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Bin Tang ◽  
ShenHao Xie ◽  
GuanLin Huang ◽  
ZhiGang Wang ◽  
Le Yang ◽  
...  

OBJECTIVETransinfundibular craniopharyngioma (TC) is one of the 4 subtypes of suprasellar craniopharyngioma. In this study, the authors analyzed the clinical features of and operative technique for TC.METHODSA total of 95 consecutive cases of suprasellar craniopharyngioma that had been resected via the endoscopic expanded endonasal approach were retrospectively reviewed. Patients were divided into 2 groups: 34 in the TC group and 61 in the nontransinfundibular craniopharyngioma (NC) group. Clinical and radiographic features, intraoperative findings, histopathological and genetic findings, and surgical outcomes were analyzed and compared between groups.RESULTSCompared with NC, TC was mostly seen in adult patients (97.1%); it was rare in children (2.9%). Clinical presentations tended toward headache, hydrocephalus, and diabetes insipidus. The relatively smaller volume, midline location (consistent with the stalk position), unidentifiable stalk, no shift of the third ventricle, and greater likelihood to involve the third ventricle and cause hydrocephalus were the characteristic features of TC in the preoperative MRI study. According to the degree of vertical extension of the tumor, the 34 TCs could be classified into 3 subtypes: type 1, entity was limited to stalk (n = 2, 5.9%); type 2, tumor extended up to the third ventricle (type 2a) or down to the subdiaphragmatic cavity (type 2b) (n = 23, 67.6%); and type 3, tumor extended in both directions (n = 9, 26.5%). For TC resection, the chiasm–pituitary corridor, lamina terminalis corridor, and pituitary corridor could be used separately or jointly. Most of the TCs originated from the infundibulum–tuber cinereum, grew within and along the long axis of the infundibulum, and the pituitary stalk was not usually preserved in TCs (20.6%), whereas the rate of preservation was higher (80.3%) in NCs. Bilateral hypothalamic injury was found in nearly all TCs if radical resection was performed, whereas the relationship between NCs and hypothalamus was either compression (32.8%) or unilateral invasion (67.2%). Meanwhile, the postoperative endocrine and neuropsychological function outcomes in patients with TC were worse than in patients with NC. The genetic analysis with whole-exome sequencing studies showed no differential mutations of CTNNB1 (β-catenin) and BRAF (V600E) between TC and NC subtypes, but there was a difference between adamantinomatous craniopharyngioma and papillary craniopharyngioma.CONCLUSIONSTC is a special subtype of suprasellar craniopharyngioma, which is remarkably different from NC. Identification of this type of tumor preoperatively is essential for the planning of appropriate surgical approach and degree of excision.


1998 ◽  
Vol 273 (17) ◽  
pp. 10806
Author(s):  
Pavel Belan ◽  
Julie Gardner ◽  
Oleg Gerasimenko ◽  
Chris Lloyd Mills ◽  
Ole H. Petersen ◽  
...  

2000 ◽  
Vol 275 (13) ◽  
pp. 9890-9891
Author(s):  
Xibao Liu ◽  
Weiching Wang ◽  
Brij B. Singh ◽  
Timothy Lockwich ◽  
Julie Jadlowiec ◽  
...  

1965 ◽  
Vol 20 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Irmgard Ziegler ◽  
M. Feron

In the eyes of male and female flies a quantitative determination of the tetrahydropterin, the corresponding dihydro-compound (Sepiapterin) and of xanthommatin from 0—5 days after hatching was made. The increase of xanthommatin almost stops 24 hours after hatching; about 13 μg are present after that time. The tetrahydropterin, after a drastic increase during the first 24 hours, remains at a level of about 1,1 — 1,2 µg. In contrast the dihydrocompound which is nearly absent at the time of hatching is deposited on the eye-granules between the third and fifth day. At that time, especially in the male the dihydropterin/tetrahydropterin ratio is increased. Starvation during the first 24 hours has essentially no effect on the amount of xanthommation whereas the amount of pterins is markedly reduced.


1998 ◽  
Vol 77 (10) ◽  
pp. 1807-1816 ◽  
Author(s):  
H. Yamaki ◽  
K. Morita ◽  
S. Kitayama ◽  
Y. Imai ◽  
K. Itadani ◽  
...  

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