Chromosome studies in 2n = 14 and 2n = 16 types of Medicago murex

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 159-163 ◽  
Author(s):  
A. Mariani ◽  
E. Falistocco

Karyological and morphological studies of Medicago murex with 2n = 16 and 2n = 14 chromosomes were undertaken. Analysis of the somatic chromosomes, using both conventional staining techniques and C-banding, showed that the 2n = 14 karyotype derived from the 2n = 16 karyotype. Plant morphology of the two types differs in relation to the characters of the leaves, florets, and pods. Attempts to obtain hybrids between the two types were completely unsuccessful. From these results it appears that the two M. murex types are not as closely related as has been usually considered and that they should be regarded as two different species.Key words: Medicago murex, chromosome number, C-banding.

2005 ◽  
Vol 3 (2) ◽  
pp. 285-290 ◽  
Author(s):  
Caroline Garcia ◽  
Orlando Moreira Filho

Karyotypes and other chromosomal markers were investigated in three species of the catfish genus Pimelodus, namely P. fur, P. maculatus and Pimelodus sp., from municipality of Três Marias, Minas Gerais, Brazil, using differential staining techniques (C-banding, Silver nitrate and CMA3 staining). The diploid chromosome number was 2n = 56 in P. maculatus and Pimelodus sp., while in P. fur 2n = 54. The karyotype of P. fur consisted in 32M + 8SM + 6ST + 8A with fundamental number (NF) of 100, that of P. maculatus 32M + 12SM + 12A with NF = 112, and that of Pimelodus sp. had 32M + 12Sm + 6ST + 6A with NF = 106.The nucleolar organizer regions (NORs) in all three species were invariably detected in telomeres of longer arm of the 20th chromosome pair. These sites were also positive after CMA3 and C-banding. No heteromorphic sex chromosomes were detected and C-banding pattern was species specific. Inferences about the karyotype differentiation in Pimelodus and putative chromosomal rearrangements are hypohesized.


1985 ◽  
Vol 27 (6) ◽  
pp. 689-696 ◽  
Author(s):  
A. Fominaya ◽  
N. Jouve

The characterization of reciprocal interchanges between chromosomes of wheat in the progeny of the backcross ((T. aestivum H-53 × S. cereale) × T. aestivum H-53) using Feulgen and C-banding staining techniques has been attempted. The translocated chromosomes were studied in detail in three lines using monosomic analysis. In the F1 of the crosses between these lines and the 21 monosomic lines of 'Chinese Spring' a total of five interchanges were identified. Using a Giemsa C-banding procedure it was possible to confirm the identity of the interchange chromosome pairs that were previously identified by monosomic analysis. Moreover, a total of 12 new interchanges were characterized or identified. C-banding also facilitated the identification of the arms involved in translocations and permitted the quantitative analysis of each multivalent in multiple interchange heterozygotes. The comparative availability of Feulgen and C-banding in studies of cytogenetic structure in wheat is discussed.Key words: C-banding, meiosis, heterochromatin, translocations, wheat, Triticum.


Euphytica ◽  
1972 ◽  
Vol 21 (2) ◽  
pp. 171-180 ◽  
Author(s):  
G. J. Speckmann ◽  
G. E. Van Dijk

2017 ◽  
Vol 9 (2) ◽  
pp. 263-273
Author(s):  
Suma KRISHNASWAMY ◽  
Bopaiah A. KUSHALAPPA

Does the adaptive plant morphology actually hint at its potential bioactive profile? To understand and decipher this, a micro-morphological investigation was conducted on an arid zone plant. Andrographis serpyllifolia (Rohl.ex.vahl.) Wight is a slightly bitter, acrid endemic herb with fair history of ethno-botanical use among different tribes of peninsular India. A. serpyllifolia was a highly evolved geophyte well-suited for high survivability in extremely harsh terrain. This plant was found to grow and perpetuate successfully under high-stress conditions of water deficit, high soil and atmospheric temperatures, poor nutrition and constant threat from herbivores. Under such circumstances, this plant possessed the potential to develop morphological adaptations that produce and accumulate a wide range of phytochemicals that could preserve, protect and defend its plant body. The twin objectives of this study were to investigate the micro-morphological features and their functional advantage that enabled the plant to flourish in adverse conditions and interpret by deductive reasoning, the potential phytochemical array of this plant given the observed features. Scanning electron microscope was used to explore surface morphologies of various vegetative and floral parts. Key findings of this micro-morphological study were presence of numerous diacytic stomata on both adaxial and abaxial leaf surfaces, abundant glandular sessile trichomes on abaxial leaf surfaces, reticulate pollen ornamentation with echinate sulcus outlined with smooth morus and deeply reticulate, highly pitted spermoderm or seed testa reminiscent of human brain. These three features may serve as pharmacognostic markers aiding in accurate identification and quality control of this herb.


2005 ◽  
Vol 28 (3) ◽  
pp. 390-393 ◽  
Author(s):  
Alayne Magalhães Trindade Domingues ◽  
Ana Maria Waldschmidt ◽  
Sintia Emmanuelle Andrade ◽  
Vanderly Andrade-Souza ◽  
Rogério Marco de Oliveira Alves ◽  
...  

2006 ◽  
Vol 96 (2) ◽  
pp. 209-212 ◽  
Author(s):  
Pedro H. B. Pierozzi ◽  
Carmem S. Fontanetti

This work presents the description and chromosome number of Urostreptus atrobrunneus sp. nov. The genus until now had not been registered yet in the São Paulo State, Brazil. The meiotic analysis showed that the species presents 2n=24, XY. The C-banding revealed large blocks of constitutive heterochromatin and two heteromorphic chromosomal pairs, one of them corresponding to the sexual pair.


CYTOLOGIA ◽  
2010 ◽  
Vol 75 (4) ◽  
pp. 443-449 ◽  
Author(s):  
Raghavan Ramakrishnan Nair ◽  
Karur Nallappagounder Shiva ◽  
Srambikkal Anchu ◽  
Thondiath John Zachariah

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 818-821 ◽  
Author(s):  
G. Röder ◽  
K. E. Linsenmair ◽  
I. Nanda ◽  
M. Schmid

The karyotype of male and female Hemilepistus elongatus was investigated by means of C-banding. The diploid chromosome number in both sexes is 2n = 50. By scrutinizing general morphology and localization of the constitutive heterochromatin, no heteromorphic sex chromosomes were found. All chromosome pairs in males are well paired during diakinesis. Hybridization of genomic DNA with (GACA)4 and (GATA)4 oligonucleotides revealed no sex-specific patterns. Key words : karyotype, C-banding, sex determination, simple DNA-repeats, Isopoda.


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