Screening of durum wheats for pasta-making quality with monoclonal antibodies for gliadin 45

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1096-1099 ◽  
Author(s):  
N. K. Howes ◽  
M. I. Kovacs ◽  
D. Leisle ◽  
M. R. Dawood ◽  
W. Bushuk
Keyword(s):  
Monoclonal Antibodies ◽  
Durum Wheat ◽  
Immunosorbent Assay ◽  
Sodium Dodecyl ◽  
Wheat Breeding ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gluten Strength ◽  
High Sodium ◽  
Sedimentation Volume

Monoclonal antibodies specific for gliadin band 45 (gli 45) of common wheat cultivar 'Marquis' were treated against durum wheat gliadins using an enzyme-linked immunosorbent assay (ELISA). In a test of 15 durum cultivars, high ELISA values were associated with gli 45, high gluten strength, and high sodium dodecyl sulfate (SDS) sedimentation volume; low ELISA values were associated with gli 42, low gluten strength, and low SDS-sedimentation volume. Analysis of 120 F5 lines from the backcrosses Ward/Vic//Vic and Ward/Vic//Ward confirmed that a high reaction to ELISA was statistically correlated with the presence of gli 45, with high gluten strength, and with high SDS-sedimentation volume. It was concluded that monoclonal antibodies specific for gli 45 have potential as a test for rapid screening of durum wheat breeding populations for desirable pasta-making quality.Key words: monoclonal antibodies, gliadin, enzyme-linked immunosorbent assay, durum wheat.

Gluten index compared with SDS-sedimentation volume for early generation selection for gluten strength in durum wheat

2010 ◽  
Vol 90 (1) ◽  
pp. 1-11 ◽  
Author(s):  
F R Clarke ◽  
J M Clarke ◽  
N A Ames ◽  
R E Knox ◽  
R J Ross
Keyword(s):  
Durum Wheat ◽  
Protein Concentration ◽  
Triticum Turgidum ◽  
Sodium Dodecyl ◽  
Field Trials ◽  
Gluten Strength ◽  
Sedimentation Volume ◽  
Selection For ◽  
Highly Correlated ◽  
Gluten Index

Gluten strength is an important end-use quality factor in durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn.], affecting pasta manufacture and cooking quality. The objective of this research was to determine the inheritance and heritability of gluten index in comparison with the widely used SDS-sedimentation (sodium dodecyl sulphate) technique for selection for gluten strength. Seven durum populations were grown in replicated, multi-location, multi-year field trials in Saskatchewan, Canada, during the period 1995 to 2002. Gluten index and SDS-sedimentation volume were determined on all plots after harvest. Both traits were affected by genotype, and to a lesser extent by year or location. Genotype environmental interactions were generally minor. The majority of genotypes in each population had similar relative ranking for gluten index and SDS-sedimentation volume in each environment. Both traits were highly heritable, ranging from 0.80 to 0.97, and both were complexly inherited with estimates of effective factors ranging from 3 to 21. Gluten index and SDS-sedimentation volume were highly correlated, indicating that they are measuring similar aspects of gluten strength. SDS-sedimentation volume was positively associated with protein concentration (r2 = 0.52), but gluten index was not. Therefore, gluten index would be more desirable than SDS-sedimentation volume for use in selection where there are within-trial protein concentration trends.Key words: Gluten strength, SDS-sedimentation, gluten index, heritability, inheritance

scholarly journals Quality characteristics of registered cultivars and advanced lines of durum wheats grown in different ecological regions of Turkey

2011 ◽  
Vol 91 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Mehmet Sakin ◽  
Abdulvahit Sayaslan ◽  
Oral Duzdemir ◽  
Ferhat Yuksel
Keyword(s):  
Durum Wheat ◽  
Sodium Dodecyl ◽  
Quality Parameters ◽  
Quality Characteristics ◽  
Wheat Breeding ◽  
Wheat Genotypes ◽  
Ecological Regions ◽  
Yield And Quality ◽  
Sedimentation Volume ◽  
Grain Yield And Quality

Sakin, M. A., Sayaslan, A., Duzdemir, O. and Yuksel, F. 2011. Quality characteristics of registered cultivars and advanced lines of durum wheats grown in different ecological regions of Turkey. Can. J. Plant Sci. 91: 261–271. In this study, pasta-quality-associated characteristics of 25 durum wheat genotypes were investigated. Durum wheat genotypes consisted of 13 advanced experimental lines and 12 registered cultivars that were grown in three different locations of Turkey for 2 yr. Genotype, location, year and their interactions were found to be statistically significant in terms of all investigated quality characteristics. Mean quality parameters for the genotypes varied as follows: yellowberry kernel 1.7–6.1%, pigment content 3.67–8.31 mg kg−1, lipoxygenase (LOX) activity 12.9–27.9 EU g−1, protein content 10.8–11.8% (14% mb), gluten index (GI) 12-61, sodium dodecyl sulphate-sedimentation volume 17.3–28.7 mL, specific sedimentation volume 1.60–2.52 mL. Of the genotypes, 10 contained γ-gliadin 45 and six γ-gliadin 42 proteins. Except for Zenit, none of the registered cultivars and advanced experimental lines investigated in this study were able to meet simultaneously the requirements for high-quality pasta products; yet certain experimental lines prevailing in specific quality characteristics, such as Line-Gdem-2, Line-Gdem-12 and Line-20, may be used for further breeding purposes. The results of this study also imply that grain yield and quality should be taken collectively into consideration in wheat breeding programs.

scholarly journals Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies for Direct Serotyping of Enteric Adenoviruses in Feces

1991 ◽  
Vol 65 (5) ◽  
pp. 552-558 ◽  
Author(s):  
Kenji TAKAGI ◽  
Yasutaka YAMASHITA ◽  
Hiroo INOUYE ◽  
Mitsuaki OHSETO ◽  
Hiroko KUWABARA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enteric Adenoviruses

A comparison of some small-scale tests for bread-making quality used in wheat breeding

1980 ◽  
Vol 95 (1) ◽  
pp. 29-34 ◽  
Author(s):  
J. A. Blackman ◽  
A. A. Gill
Keyword(s):  
Protein Quality ◽  
Small Sample Size ◽  
Sodium Dodecyl ◽  
Wheat Breeding ◽  
Small Sample ◽  
Small Scale ◽  
Loaf Volume ◽  
Bread Making ◽  
Wheat Varieties ◽  
Sedimentation Volume

SummaryTwenty-five winter wheat varieties and breeders' lines including hard and soft texture, good or poor bread and biscuit-making types were grown at two locations in the U.K. in 1977 to provide the test samples. Small-scale tests of bread-making quality including extensometer, sodium dodecyl sulphate (SDS) sedimentation volume, residue protein, urea dispersible protein and Pelshenke tests, were compared with loaf volumes and loaf scores.Averaged over the two sites, a modified extensometer test and the SDS test gave the closest correlation with loaf volume and loaf score and were only poorly correlated with Hagberg Falling Number and percentage protein. The SDS test gave the closest correlation between sites followed by the extensometer readings; loaf volume and score had much lower values. The SDS values and extensometer readings give a better measure of the genetic differences in protein quality of varieties than loaf volume and score, being less affected by growing conditions. With its small sample size and high throughput, the SDS sedimentation volume is likely to be the most useful screening test for wheat breeding programmes.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies

1989 ◽  
Vol 24 (1-2) ◽  
pp. 43-56 ◽  
Author(s):  
Giuseppe Gerna ◽  
Antonella Sarasini ◽  
Angela Di Matteo ◽  
Maurizio Parea ◽  
Maria Torseilini ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Rotavirus
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