Recombination in yeast and the recombinant DNA technology

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 536-540 ◽  
Author(s):  
Thomas D. Petes ◽  
Peter Detloff ◽  
Sue Jinks-Robertson ◽  
S. Renee Judd ◽  
Martin Kupiec ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Recombinant Dna ◽  
Recombinant Dna Technology ◽  
Yeast Saccharomyces Cerevisiae ◽  
Eukaryotic Genes ◽  
Dna Technology ◽  
Recombinant Dna Techniques ◽  
Repeated Genes

The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have been obtained in these studies.Key words: recombination, Saccharomyces cerevisiae, gene conversion, repeated genes.

Microbial Production of Recombinant Rennet

2018 ◽  
pp. 222-233 ◽  
Author(s):  
Zumrut Begum Ogel
Keyword(s):  
Proteolytic Activity ◽  
Recombinant Dna ◽  
Microbial Production ◽  
Recombinant Dna Technology ◽  
Aspartic Proteases ◽  
The Past ◽  
Traditional Cheese ◽  
Dna Technology ◽  
Cheese Products ◽  
Recombinant Dna Techniques

Rennet, traditionally obtained from calves, is non-vegeterian and unethical due to the slaughter of unweaned animals. Chymosin is highly specific to the Phe105-Met106 bond of κ-casein and has low proteolytic activity. Microbial aspartic proteases can partly replace chymosin. However, recombinant DNA technology has allowed chymosin itself to be produced by bacteria, yeast, and molds. Not only rennet from calf, but from animals like goat kid, lamb, buffalo, camel, and others can be used in cheesemaking. Chymosins of these animals can be cloned and successfully expressed in microorganisms and can be employed in the production of novel as well as traditional cheese products from the milk of camel, goat, and even horse and donkey. This chapter outlines the recombinant DNA techniques applied over the past few years to improve the microbial production of recombinant rennet, from animals and plants.

The application of recombinant-DNA technology to cellulases and lignocellulosic wastes

1987 ◽  
Vol 321 (1561) ◽  
pp. 449-454 ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Hydrolysis ◽  
Trichoderma Reesei ◽  
Recombinant Dna ◽  
Full Length ◽  
Efficient Utilization ◽  
Recombinant Dna Technology ◽  
Lignocellulosic Wastes ◽  
Dna Technology

The efficient utilization of different lignocellulosic wastes will require an understanding of the roles of the different enzymes involved in enzymatic hydrolysis. Towards this goal, we have isolated and characterized the genes coding for four major cellulases, CBH I, CBH II, EG I and EG III, produced by the cellulolytic fungus Trichoderma reesei . It seems that T. reesei produces at least two classes of cellulose and in each class both endo- and exo-type enzyme are found. Full-length cDNAs coding for CBH I, CBH II, EG I and EG III have been expressed in the yeast Saccharomyces cerevisiae , and the cellulases are secreted by these strains. The recombinant cellulases produced by the yeast all show activity towards cellulosic substrates. The information obtained from the cloned genes makes possible the construction of new cellulolytic organisms and may also make possible the development of improved enzymes.

scholarly journals NGO war on biotechnology

2005 ◽  
Vol 11 (3) ◽  
Author(s):  
Henry I Miller ◽  
Gregory Conko
Keyword(s):  
Genetic Modification ◽  
Recombinant Dna ◽  
Molecular Genetic ◽  
Significant Risk ◽  
Environmental Safety ◽  
Recombinant Dna Technology ◽  
Risks And Benefits ◽  
Dna Technology ◽  
Recombinant Dna Techniques ◽  
The Cost

Discussions of the risks and benefits of recombinant DNA technology, or 'genetic modification' (GM), should occur within the context of experience with older, 'conventional' techniques for genetic improvement. But critics' alarmist reports and commentaries invariably emphasise the things that might go wrong only with recombinant DNA-modified organisms, while studiously avoiding the essential broader context. They ignore vast amounts of data, including literally millennia of experience with less precise methods used for genetic modification, and they continue to deny the well-established scientific consensus that no unique risks attend the use of recombinant DNA techniques. They promulgate the perception that recombinant DNA technology is unproven, untested and unregulated – and promote an approach to regulation in which there is an inverse relationship between degree of scrutiny and risk. The disproportionate regulation of the products of recombinant DNA technology needlessly raises the cost of research and development, while it fails to advance consumer or environmental safety. The question we must ask is not whether regulation generally is or is not justified, but rather what should be regulated and how? The use of certain techniques – in particular, those that are the most precise and predictable – as a trigger for regulation cannot be justified scientifically. Regulatory efforts should be redirected to focus oversight on new organisms that express characteristics likely to pose significant risk, regardless of the methods used in their development, while leaving relatively low-risk traits of both classical and molecular genetic modification unburdened by costly regulation.

scholarly journals CHROMOSOMAL TRANSLOCATIONS GENERATED BY HIGH-FREQUENCY MEIOTIC RECOMBINATION BETWEEN REPEATED YEAST GENES

Genetics ◽  
1986 ◽  
Vol 114 (3) ◽  
pp. 731-752
Author(s):  
Sue Jinks-Robertson ◽  
Thomas D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Mitotic Recombination ◽  
Chromosomal Translocations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes ◽  
Yeast Genes ◽  
Repeated Genes

ABSTRACT We have examined meiotic and mitotic recombination between repeated genes on nonhomologous chromosomes in the yeast Saccharomyces cerevisiae . The results of these experiments can be summarized in three statements. First, gene conversion events between repeats on nonhomologous chromosomes occur frequently in meiosis. The frequency of such conversion events is only 17-fold less than the analogous frequency of conversion between genes at allelic positions on homologous chromosomes. Second, meiotic and mitotic conversion events between repeated genes on nonhomologous chromosomes are associated with reciprocal recombination to the same extent as conversion between allelic sequences. The reciprocal exchanges between the repeated genes result in chromosomal translocations. Finally, recombination between repeated genes on nonhomologous chromosomes occurs much more frequently in meiosis than in mitosis.

MAP POSITIONS OF YEAST GENES SIR1, SIR3 and SIR4

Genetics ◽  
1985 ◽  
Vol 111 (4) ◽  
pp. 735-744
Author(s):  
John M Ivy ◽  
James B Hicks ◽  
Amar J S Klar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Recombinant Dna ◽  
Yeast Saccharomyces Cerevisiae ◽  
Recombinant Dna Techniques ◽  
The Right ◽  
Yeast Genes ◽  
Type Information

ABSTRACT The HML and HMR loci in the yeast Saccharomyces cerevisiae each contain a complete copy of mating-type information. HML and HMR normally are transcriptionally inactive due to four unlinked genes, known as MAR or SIR or CMT. The map position of MAR1 (SIR2) has been reported previously; it is located on the left arm of chromosome IV, 27 cM from the centromere. Using conventional meiotic and mitotic mapping combined with recombinant DNA techniques, we have mapped three other SIR genes. SIR1 maps near the telomere of the right arm of chromosome XI; SIR3 (MAR2) maps to the right arm of chromosome XII, 31 cM distal to URA4; and SIR4 maps to the right arm of chromosome IV, 16 cM proximal to LYS4.

scholarly journals Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites

2021 ◽  
Author(s):  
Mahsa Babaei ◽  
Luisa Sartori ◽  
Alexey Karpukhin ◽  
Dmitrii Abashkin ◽  
Elena Matrosova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Dna ◽  
Green Fluorescence Protein ◽  
Cellular Growth ◽  
Green Fluorescence ◽  
Biotechnological Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Integration Sites ◽  
Fluorescence Protein ◽  
Integration Efficiency

Abstract Biotechnological production requires genetically stable recombinant strains. To ensure genomic stability, recombinant DNA is commonly integrated into the genome of the host strain. Multiple genetic tools have been developed for genomic integration into baker's yeast Saccharomyces cerevisiae. Previously, we had developed a vector toolkit EasyClone-MarkerFree for stable integration into eleven sites on chromosomes X, XI, and XII of S. cerevisiae. The markerless integration was enabled by CRISPR-Cas9 system. In this study, we have expanded the kit with eight additional intergenic integration sites located on different chromosomes. The integration efficiency into the new sites was above 80%. The expression level of green fluorescence protein (gfp) for all eight sites was similar or above XI-2 site from the original EasyClone-MarkerFree toolkit. The cellular growth was not affected by the integration into any of the new eight locations. The eight-vector expansion kit is available from AddGene.

scholarly journals The Highly Conserved tRNAHis Guanylyltransferase Thg1p Interacts with the Origin Recognition Complex and Is Required for the G2/M Phase Transition in the Yeast Saccharomyces cerevisiae

2005 ◽  
Vol 4 (4) ◽  
pp. 832-835 ◽  
Author(s):  
Terri S. Rice ◽  
Min Ding ◽  
David S. Pederson ◽  
Nicholas H. Heintz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Origin Recognition Complex ◽  
Nuclear Division ◽  
Permissive Temperature ◽  
Yeast Saccharomyces Cerevisiae ◽  
M Phase ◽  
And Migration ◽  
Origin Recognition

ABSTRACT Here we show that the Saccharomyces cerevisiae tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex in vivo and in vitro and that overexpression of hemagglutinin-Thg1p selectively impedes growth of orc2-1(Ts) cells at the permissive temperature. Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast.

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