A comparison of the karyotypes of six species of Charadriiformes

Genome ◽  
1987 ◽  
Vol 29 (2) ◽  
pp. 213-220
Author(s):  
Sylvia E. Bartlett ◽  
William Threlfall
Keyword(s):  
Key Words ◽  
Fluorescent Dye ◽  
Larus Argentatus ◽  
Embryonic Tissue ◽  
Mitotic Chromosomes ◽  
Alca Torda ◽  
Rissa Tridactyla ◽  
Primary Explants ◽  
Larus Marinus

The embryonic tissue of three larid species (Larus argentatus, Larus marinus, Rissa tridactyla) and three species of alcids (Uria aalge, Alca torda, Fraterula arctica) were grown in vitro as primary explants to obtain mitotic chromosomes. Chromosomes that were harvested were stained with the fluorescent dye Atebrin. Partial karyotypes and idiograms of the largest 13 autosomes were made for each species. Based on the p arm to q arm ratios the centromere position for each chromosome was determined. Comparisons were then made between larid and alcid species as well as between groups (larid-alcid) and significant differences were noted. Key words: karyotypes, gulls, auks, Charadriiformes.

scholarly journals CHANGES IN POPULATIONS OF NESTING SEABIRDS ON THE BIRD ISLANDS, CAPE BRETON, NOVA SCOTIA

2004 ◽  
Vol 42 (2) ◽  
Author(s):  
David B. McCorquodale ◽  
Dan B. Banks ◽  
Matthew I. Kerr ◽  
Richard W. Knapton ◽  
David L. Harris
Keyword(s):  
Nova Scotia ◽  
Larus Argentatus ◽  
Phalacrocorax Carbo ◽  
Alca Torda ◽  
Rissa Tridactyla ◽  
The Past ◽  
Fratercula Arctica ◽  
Breeding Populations ◽  
Sterna Paradisaea ◽  
Great Cormorants

The Bird Islands support the largest colonies of nesting seabirds in Nova Scotia. From a continental perspective the islands host one of the largest Great Cormorant (Phalacrocorax carbo) colonies in North America and a significant Razorbill (Alca torda) colony. However the islands are best known for nesting Atlantic Puffins (Fratercula arctica). Populations of all species have changed over the past 75 years, none so dramatically as Black-legged Kittiwakes (Rissa tridactyla). Kittiwakes colonized the islands 20 years ago and are now the most numerous nesting seabirds. Two other recent colonists, Herring (Larus argentatus) and Great Black-backed Gulls (L. marinus) are the next most common nesting species on the islands. In contrast the small colony of Common Murres (Uria aalge) and the large colonies of Arctic (Sterna paradisaea) and Common Terns (S. hirundo) of the 1920s have disappeared. Here we present recent estimates of breeding populations and summarize the data on changes in populations in the past 75 years based on a review of the literature, and surveys conducted from boats circumnavigating the islands during 11 different years from 1976 until 2000. The changes in breeding populations of seabirds on the Bird Islands show that their importance for conservation has changed through the years. For example in the 1920s about one third of all nesting Double-crested Cormorants in Nova Scotia were on the Bird Islands. In 1933 no Great Cormorants nested on the islands. In contrast in 2000 they supported the largest colony of Great Cormorants in North America and less than 1% of the Double-crested Cormorants in eastern Canada.En Nouvelle-Écosse, ce sont les îles Bird qui abritent les plus grosses colonies d’oiseaux de mer nicheurs. Les îles hébergent une des plus grosses colonies de Grands Cormorans (Phalacrocorax carbo) de l’Amérique du Nord et une colonie importante de Petits Pingouins (Alca torda). Cependant, les îles sont plus connues pour les Macareux moines (Fratercula arctica). Depuis les 75 dernières années, les populations de toutes les espèces ont changé, mais c’est la population de Mouettes tridactyles (Rissa tridactyla) qui a changé le plus. Il y a vingt ans, ces mouettes ont colonisé les îles et sont devenues les oiseaux de mer les plus abondants à s’y reproduire. Deux autres espèces ayant colonisé les îles depuis peu, le Goéland argenté (Larus argentatus) et le Goéland marin (L. marinus), arrivent au second rang pour ce qui est des oiseaux nicheurs les plus nombreux sur les îles. Par contre, la petite colonie de Guillemots marmettes (Uria aalge) et les grosses colonies de Sternes arctiques (Sterna paradisaea) et de Sternes pierregarins (S. hirundo) qui y vivaient durant les années 1920 ont disparu. Nous présentons ici des évaluations récentes des populations d’oiseaux nicheurs et les tendances démographiques depuis les 75 dernières années à partir d’une revue de la documentation, ainsi que les résultats de relevés effectués dans 11 années, entre 1976 et 2000, à bord de bateaux qui ont fait le tour des îles. Les changements dans les populations d’oiseaux de mer qui se reproduisent sur les îles Bird montrent que leur importance sur le plan de la conservation a varié au cours des années. Par exemple, dans les années 1920, environ un tiers de tous les Cormorans à aigrettes qui nichaient en Nouvelle-Écosse le faisaient sur les îles Bird. En 1933, aucun Grand Cormoran ne nichait sur les îles, tandis qu’en 2000, elles abritaient la plus grosse colonie de cette espèce en Amérique du Nord et moins de 1 % des Cormorans à aigrettes de l’est du Canada.PROC. N.S. INST. SCI. (2004)Volume 42, Part 2, pp. 241-252*

Giemsa as a fluorescent dye for mineralizing bone-like nodules in vitro

2012 ◽  
Vol 7 (1) ◽  
pp. 011001 ◽  
Author(s):  
W Querido ◽  
M Farina ◽  
A Balduino
Keyword(s):  
Fluorescent Dye

scholarly journals GLUCOSE CONSUMPTION AND LACTIC ACID PRODUCTION BY ERYTHROCYTES FROM DOUBLE-MUSCLED CATTLE

1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON
Keyword(s):  
Lactic Acid ◽  
Key Words ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Age Groups ◽  
Phenotypic Expression ◽  
Glucose Consumption ◽  
Molar Ratio ◽  
Breed Group

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid

Results of an experimental study of hydrogel drainage containing ranibizumab in antiglaucoma operations

2021 ◽  
pp. 63-67
Author(s):  
I.I. Khusnitdinov ◽  
Keyword(s):  
Experimental Study ◽  
Hyaluronic Acid ◽  
Key Words ◽  
Experimental Research ◽  
Experimental Studies ◽  
Glaucoma Surgery ◽  
Control Group ◽  
Morphological Studies

Purpose. Еxperimental substantiation of the effectiveness of biocompatible biodegradable hydrogels based on hyaluronic acid and chitosan succinate as a carrier of ranibizumab in antiglaucoma operations. Material and methods. Hydrogel drainage (HD) was obtained immediately before surgery. A solution of ranibizumab (0.23 ml) was mixed with a solution of hyaluronic acid dialdehyde (0.5 ml), then a solution of chitosan succinate (0.5 ml) was added. Experimental studies were performed in 12 (12 eyes) healthy rabbits. The first group consisted of 6 eyes – 0.187 ml of ranibizumab per 1 ml of gel. In the control group, HD was used intraoperatively without the addition of ranibizumab (6 eyes). Morphological studies were performed on 7th, 21st, and 42nd days. Results. In experimental studies in vitro and in vivo, it was proved that ranibizumab, administered as a part of 0.1 ml of hydrogel drainage in the antiglaucoma surgery area is released within 3 weeks and suppresses vascularization, scarring of the operating area, and preserves the intrascleral cavity. The optimal concentration of ranibizumab was selected-0.02 ml in 0.1 ml of gel. Conclusion. The safety and effectiveness of the use of hydrogel drainage with ranibizumab based on hyaluronic acid dialdehyde and chitosan succinate in anti-glaucoma operations has been proven. Key words: experimental research, hydrogel drainage, ranibizumab, glaucoma surgery.

scholarly journals Effects of Growth Regulators on In vitro Propagation and Tuberization of Four Dioscorea Species

1970 ◽  
Vol 18 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Md. Tariqul Islam ◽  
E. R. Joachim Keller ◽  
D. Philibert Dembele
Keyword(s):  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Production ◽  
Dioscorea Species ◽  
Vitro Production ◽  
Species Being ◽  
Nodal Culture

Nodal explants of 12 accessions from four species of yam (Dioscorea spp.) were cultured for six weeks on MS to evaluate the influence of IAA, Kn, NAA and BAP on the production of leaves and microtubers. Four Dioscorea polystachya Turcz., three each of D. bulbifera L. and D. sansibarensis Pax. and two D. japonica Thunb. accessions were used. Five and 10 mg/l of Kn along with IAA and sucrose, and 0.2 and 0.5 mg/l of NAA, sucrose and with or without BAP were used in four treatments. The accessions Yam 23 and Yam 25 of D. sansibarensis failed to initiate any leaf under four treatments. The remaining accessions produced 0.11 to 1.76 leaves per explant. The medium containing IAA with higher concentration of Kn (10 mg/l) and 3% sucrose was found to be best for in vitro production of leaf (0.71/explant) and the most productive species was D. japonica (1.36), followed by D. polystachya (1.19/explant). At the same culture period, Yam 16 of D. bulbifera failed to initiate any microtuber at IAA with Kn, and NAA with or without BAP. The remaining accessions produced 0.09 to 1.15 microtubers per explant. Lower concentration of Kn (5 mg/l) with IAA and sucrose was favourable for producing microtubers (0.61/explant on an average), the best species being D. sansibarensis (1.27) followed by D. japonica (0.59/ explant). Finally, the presence of BAP adversely affected the production of microtuber among Dioscorea species. Key words: Dioscorea spp., Microtuber, Nodal culture, Propagation D.O.I. 10.3329/ptcb.v18i1.3260 Plant Tissue Cult. & Biotech. 18(1): 25-35, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals Triple-Helical DNA in Drosophila Heterochromatin

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 227 ◽  
Author(s):  
Eduardo Gorab
Keyword(s):  
Nucleic Acids ◽  
Dna Sequences ◽  
Detection Methods ◽  
Triplex Dna ◽  
Thiazole Orange ◽  
Chromosomal Dna ◽  
Mitotic Chromosomes ◽  
Satellite Repeats

Polynucleotide chains obeying Watson-Crick pairing are apt to form non-canonical complexes such as triple-helical nucleic acids. From early characterization in vitro, their occurrence in vivo has been strengthened by increasing evidence, although most remain circumstantial particularly for triplex DNA. Here, different approaches were employed to specify triple-stranded DNA sequences in the Drosophila melanogaster chromosomes. Antibodies to triplex nucleic acids, previously characterized, bind to centromeric regions of mitotic chromosomes and also to the polytene section 59E of mutant strains carrying the brown dominant allele, indicating that AAGAG tandem satellite repeats are triplex-forming sequences. The satellite probe hybridized to AAGAG-containing regions omitting chromosomal DNA denaturation, as expected, for the intra-molecular triplex DNA formation model in which single-stranded DNA coexists with triplexes. In addition, Thiazole Orange, previously described as capable of reproducing results obtained by antibodies to triple-helical DNA, binds to AAGAG repeats in situ thus validating both detection methods. Unusual phenotype and nuclear structure exhibited by Drosophila correlate with the non-canonical conformation of tandem satellite arrays. From the approaches that lead to the identification of triple-helical DNA in chromosomes, facilities particularly provided by Thiazole Orange use may broaden the investigation on the occurrence of triplex DNA in eukaryotic genomes.

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