Inheritance of self-incompatibility in rutabaga (Brassica napus L. ssp. rapifera (Metzg.) Sinsk.)

1985 ◽  
Vol 27 (6) ◽  
pp. 710-715 ◽  
Author(s):  
R. Ayotte ◽  
P. M. Harney ◽  
B. R. Christie
Keyword(s):  
Brassica Napus ◽  
Microscopic Examination ◽  
Seed Set ◽  
Turnip Mosaic Virus ◽  
Second Line ◽  
Self Incompatibility ◽  
Brassica Napus L ◽  
Microscopic Evaluation ◽  
S Alleles ◽  
S Allele

Self-incompatible and self-compatible lines of Brassica napus L. were crossed and the compatibility status of the F1 and F2 progeny assessed. Compatibility was established by measuring silique and seed set and by microscopic examination of self-pollen behaviour on the stigma and within the style of flowers. Microscopic evaluation was found to be unreliable in determining whether seed set would occur on a plant following self-pollination. The F1 data could not be interpreted because turnip mosaic virus in the greenhouse killed many plants and may have affected the compatibility reaction of the rest. Silique set data for the F2 showed one line (Z) segregated 3:1 self-incompatible to self-compatible, indicating only one locus involved. The 10:6 self-compatible to self-incompatible ratio, obtained for a second line (R) can be explained in the same manner if a dominant modifier (M), which interacts only with S-allele heteozygotes, is also present.Key words: rutabaga, Brassica napus, self-incompatibility, S alleles, genetic.

Evaluation of two self-incompatibility alleles in three summer rape (Brassica napus L.) cultivars by UV fluorescence microscopy, seed set and outcrossing rates

2000 ◽  
Vol 80 (2) ◽  
pp. 255-260
Author(s):  
L. J. Lewis ◽  
D. L. Woods ◽  
H. W. Klein-Gebbinck
Keyword(s):  
Brassica Napus ◽  
Seed Set ◽  
Brassica Napus L ◽  
Significant Difference ◽  
S Alleles ◽  
Outcrossing Rates ◽  
Incompatibility Alleles ◽  
Sporophytic Incompatibility ◽  
S Allele ◽  
Yellow Petal

S-alleles W1 and T2 and an incompletely dominant white petal character were introgressed into the self-compatible (SC) summer rape (Brassica napus L. ssp. oleifera {Metzg.}) cultivars Global, Topas and Westar. The derived self-incompatible (SI) lines were evaluated for strength of incompatibility by ultraviolet fluorescence of pollen tubes, and by seed set. Pollen tube and seed set analyses showed the W1 and T2 alleles were strongly, moderately and weakly expressed in Topas, Global and Westar, respectively. Seed set data showed a significant difference between SI lines, but not between S-alleles, or between homozygous or heterozygous lines from the same SI cultivar. SI cultivar yellow petal (wild type) lines were field pollinated with SC white petal lines. Seed collected from the SI cultivars were evaluated for proportion of outcrossed progeny by recording the frequency of yellow petal and cream petal plants, which were the result of self- and cross-pollination, respectively. The proportion of outcrossed progeny (i.e., outcrossing rates) ranged from 23% to 79%. Topas SI lines had significantly higher outcrossing rates than Global SI lines, which corresponded to SI line seed set data. Environment, S-allele selection and genotype significantly affected outcrossing rates. Key words: Brassica napus, sporophytic incompatibility, S-allele, outcrossing rate

Latent S alleles are widespread in cultivated self-compatible Brassica napus

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 257-265 ◽  
Author(s):  
U U Ekuere ◽  
I A.P Parkin ◽  
C Bowman ◽  
D Marshall ◽  
D J Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Self Incompatibility ◽  
S Locus ◽  
Winter Oilseed Rape ◽  
A Genome ◽  
C Genome ◽  
S Alleles ◽  
Crop Types ◽  
S Allele

The genetic control of self-incompatibility in Brassica napus was investigated using crosses between resynthesized lines of B. napus and cultivars of oilseed rape. These crosses introduced eight C-genome S alleles from Brassica oleracea (S16, S22, S23, S25, S29, S35, S60, and S63) and one A-genome S allele from Brassica rapa (SRM29) into winter oilseed rape. The inheritance of S alleles was monitored using genetic markers and S phenotypes were determined in the F1, F2, first backcross (B1), and testcross (T1) generations. Two different F1 hybrids were used to develop populations of doubled haploid lines that were subjected to genetic mapping and scored for S phenotype. These investigations identified a latent S allele in at least two oilseed rape cultivars and indicated that the S phenotype of these latent alleles was masked by a suppressor system common to oilseed rape. These latent S alleles may be widespread in oilseed rape varieties and are possibly associated with the highly conserved C-genome S locus of these crop types. Segregation for S phenotype in subpopulations uniform for S genotype suggests the existence of suppressor loci that influenced the expression of the S phenotype. These suppressor loci were not linked to the S loci and possessed suppressing alleles in oilseed rape and non-suppressing alleles in the diploid parents of resynthesized B. napus lines.Key words: self-incompatibility, B. oleracea, B. rapa, S locus, suppression.

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

2007 ◽  
Vol 21 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Xingguo Zhang ◽  
Chaozhi Ma ◽  
Tingdong Fu ◽  
Yuanyuan Li ◽  
Tonghua Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Scar Markers ◽  
Self Incompatibility ◽  
Brassica Napus L

scholarly journals Nonself-recognition-based self-incompatibility can alternatively promote or prevent introgression

2020 ◽  
Author(s):  
Alexander Harkness ◽  
Yaniv Brandvain
Keyword(s):  
Pollen Limitation ◽  
List Type ◽  
Self Incompatibility ◽  
Core Eudicots ◽  
S Alleles ◽  
Self Fertilization ◽  
Nonself Recognition ◽  
Negative Frequency Dependent Selection ◽  
Incompatibility Alleles ◽  
S Allele

1SummaryTraditionally, we expect that self-incompatibility alleles (S-alleles), which prevent self-fertilization, should benefit from negative-frequency dependent selection and rise to high frequency when introduced to a new population through gene flow. However, the most taxonomically widespread form of self-incompatibility, the ribonuclease-based system ancestral to the core eudicots, functions through nonself-recognition, which drastically alters the process of S-allele diversification.We analyze a model of S-allele evolution in two populations connected by migration, focusing on comparisons among the fates of S-alleles originally unique to each population and those shared among populations.We find that both shared and unique S-alleles originating from the population with more unique S-alleles were usually fitter than S-alleles from the population with fewer. Resident S-alleles were often driven extinct and replaced by migrant S-alleles, though this outcome could be averted by pollen limitation or biased migration.Nonself-recognition-based self-incompatibility will usually either disfavor introgression of S-alleles or result in the whole-sale replacement of S-alleles from one population with those from another.

ALLELIC POLYMORPHISM OF THE SELFINCOMPATIBILITY GENE IN CHERRY VARIETIES IN SELECTION OF ALL-RUSSIAN RESEARCH INSTITUTE FOR FRUIT CROP BREEDING

1970 ◽  
pp. 26-28
Author(s):  
Е. V. Bezlepkina ◽  
А. А. Gulyaeva ◽  
А. V. Pikunova
Keyword(s):  
Sour Cherry ◽  
Protein S ◽  
Allelic Polymorphism ◽  
Self Incompatibility ◽  
Allele Polymorphism ◽  
Multiple Allele ◽  
S Gene ◽  
S Alleles ◽  
Self Fertilization ◽  
S Allele

Self-incompatibility is one of the most important mechanisms used by flowering plants to prevent self-fertilization and, consequently, to provide the genetic diversity of population. The self-incompatibility in Prunus is controlled by two genes as minimum: S (self-incompatibility) and SFB (S haplotype-specific F-box protein).  S gene is represented in the population by a multiple allele series. Compatibility in pollination is dependent on the S allele combination of the cultivars. When S allele in the pollen is identical with one of the S alleles of the mother plant fertilization is arrested. Thus, both self-fertilization and fertilization by pollen of closely related plants (having identical S alleles) are prevented. This mechanism may be interrupted in the case of mutations in the S or SFB gene, which leads to the appearance of self-compatibility cultivars, and polyploidization, such as in sour cherry. The investigation of the S gene allele polymorphism of sweet cherry cultivars of VNIISPK breeding was performed as a part of the study of the gene collection of stone crops. Both consensus (PaConsI, PaConsII) and allele-specific (S1, S5, S9, S10) primers were used. The S-genotype of cultivars Adelina (S3/S5), Poezia (S3/S5), Siana (S3/S6), Orlovskaia feia (S3/S5) and Trosnianskaia (S5/S6) were established. The S-genotype of cultivars Malish, Podarok Orlu, Orlovskaia rozovaia and Orlovskaia yantarnaia was determined partially, as these cultivars have unique previously undescribed or very rare S alleles. Podarok Orlu variety has S9 allele and undescribed one. Malish and Orlovskaia yantarnaia varieties have S6 allele and S17 or S30 alleles supposedly, for these alleles specific primers have not yet been developed. Orlovskaya rozovaia has S6 allele and undescribed previously one.

THE DEVELOPMENT OF Turnip mosaic virus RESISTANT DOUBLED HAPLOID LINES OF Brassica napus L.

2013 ◽  
pp. 122-125
Author(s):  
I.А. Zubareva ◽  
◽  
E.N. Goloveshkina ◽  
S.V. Vinogradova ◽  
T.N. Gribova ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Doubled Haploid ◽  
Turnip Mosaic Virus ◽  
Brassica Napus L ◽  
Doubled Haploid Lines
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