UNUSUAL VARIATIONS IN NUCLEAR DNA CONTENTS IN THE MARINE COPEPOD PSEUDOCALANUS

1982 ◽  
Vol 24 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Judith H. Robins ◽  
Ian A. McLaren
Keyword(s):  
Body Size ◽  
Nuclear Dna ◽  
Cube Root ◽  
Chromatin Diminution ◽  
Marine Copepod ◽  
Dna Contents ◽  
Eurytemora Herdmani ◽  
Nuclear Number ◽  
Mean Differences ◽  
Nuclear Dna Contents

Variations in DNA/nucleus within adult females are similar, when normalized for mean differences, in the copepods Pseudocalanus sp. and Eurytemora herdmani. However, Pseudocalanus shows great variation among individuals, with no evidence of significant groupings (species?), except perhaps for a few individuals with abnormally small DNA values. Body lengths of adult female Pseudocalanus are approximately proportional to the cube root of DNA/nucleus. It is inferred that nuclear size, not nuclear number, determines body size, and this is supported by generally similar nuclear counts in newly hatched nauplii and among first copepodid stages. Although body size and development rate of Pseudocalanus had earlier been shown to be strongly heritable, an offspring-parent regression reflected no heritability of DNA/nucleus (and some abnormally small offspring values) under the probably suboptimal conditions of rearing. Some variability in DNA content of adult somatic nuclei may be related to chromatin diminution during embryogenesis. An unusual loss of nonchromosomal, Feulgen-positive material is documented for first cleavage in Pseudocalanus, but not E. herdmani.

scholarly journals Nuclear DNA contents, rDNAs, and karyotype evolution in subgenus Vicia: III. The heterogeneous section Hypechusa

PROTOPLASMA ◽  
2006 ◽  
Vol 228 (4) ◽  
pp. 167-177 ◽  
Author(s):  
P. Caputo ◽  
M. Frediani ◽  
G. Venora ◽  
C. Ravalli ◽  
M. Ambrosio ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Karyotype Evolution ◽  
Dna Contents ◽  
Nuclear Dna Contents

Nuclear DNA Contents of Coreopsis nuecensoides and C. nuecensis (Asteraceae), a Progenitor-Derivative Species Pair

1984 ◽  
Vol 145 (2) ◽  
pp. 240-245 ◽  
Author(s):  
H. James Price ◽  
Daniel J. Crawford ◽  
Randall J. Bayer
Keyword(s):  
Nuclear Dna ◽  
Species Pair ◽  
Dna Contents ◽  
Nuclear Dna Contents

Flow cytometric analysis reveals different nuclear DNA contents in cultivated Fonio (Digitaria spp.) and some wild relatives from West-Africa

2007 ◽  
Vol 267 (1-4) ◽  
pp. 163-176 ◽  
Author(s):  
H. Adoukonou-Sagbadja ◽  
V. Schubert ◽  
A. Dansi ◽  
G. Jovtchev ◽  
A. Meister ◽  
...  
Keyword(s):  
West Africa ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Wild Relatives ◽  
Flow Cytometric ◽  
Dna Contents ◽  
Nuclear Dna Contents

Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry

2009 ◽  
Vol 57 (6) ◽  
pp. 524 ◽  
Author(s):  
Milene Miranda Praça ◽  
Carlos Roberto Carvalho ◽  
Carolina Ribeiro Diniz Boaventura Novaes
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Methodological Approach ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Eucalyptus Species ◽  
International Consensus ◽  
Mean Values ◽  
Dna Contents ◽  
Nuclear Dna Contents

Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.

scholarly journals Genome Sizes in Hepatica Mill: (Ranunculaceae) Show a Loss of DNA, Not a Gain, in Polyploids

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
B. J. M. Zonneveld
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Close Relationships ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dna Contents ◽  
Asiatic Species ◽  
Nuclear Dna Contents ◽  
Allotetraploid Species

Genome size (C-value) was applied anew to investigate the relationships within the genus Hepatica (Ranunculaceae). More than 50 samples representing all species (except H. falconeri), from wild and cultivated material, were investigated. Species of Hepatica turn out to be diploid (), tetraploid ( ), and a possible pentaploid. The somatic nuclear DNA contents (2C-value), as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14%) loss of nuclear DNA in the natural allopolyploids was found.

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