INHERITANCE OF ALLOZYME VARIANTS IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA MENZIESII VAR. MENZIESII)

1982 ◽  
Vol 24 (3) ◽  
pp. 325-335 ◽  
Author(s):  
Y. A. El-Kassaby ◽  
F. C. Yeh ◽  
O. Sziklai
Keyword(s):  
Superoxide Dismutase ◽  
Acid Phosphatase ◽  
Pseudotsuga Menziesii ◽  
Malic Enzyme ◽  
Quaternary Structure ◽  
Allelic Variation ◽  
Enzyme System ◽  
Douglas Fir ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

The inheritance of 27 loci from 18 enzyme systems was investigated using both megagametophyte and embryo tissues of open pollinated seed collected from a natural stand of coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Four enzyme systems - glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mannose-6-phosphate isomerase (MPI), and peptidase (PEP) - appeared to be monomorphic and the remaining 14 systems - acid phosphatase (APH), aconitase (ACO), aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), glucose-6-phosphate dehydrogenase (G6P), hexoseaminidase (HA), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), malic enzyme (ME), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconic dehydrogenase (6-PGD), and superoxide dismutase (SOD)-showed polymorphism. Each enzyme system was discussed with respect to its multilocus organization, subunit (quaternary) structure, and allelic variation.

Inheritance of needle tissue isozymes in Douglas-fir

1984 ◽  
Vol 26 (4) ◽  
pp. 459-468 ◽  
Author(s):  
D. B. Neale ◽  
J. C. Weber ◽  
W. T. Adams
Keyword(s):  
Glutamate Dehydrogenase ◽  
Pseudotsuga Menziesii ◽  
Isocitrate Dehydrogenase ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Glutamate Oxaloacetate Transaminase ◽  
Electrophoretic Variants ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

Methods for resolving electrophoretic variants from extracts of needle tissue of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco) are described, and the inheritance of 12 of at least 15 loci that control allozymes from 11 enzyme systems are established. Evidence for the inheritance of allozyme variants was obtained in three ways: (i) comparison in seed orchard clones of allozyme genotypes determined from both megagametophyte and needle tissue; (ii) analysis of segregating full-sib progenies of seed orchard clones; and (iii) comparison of needle allozyme pattern phenotypes to previously reported embryo phenotypes. Ten of the 12 loci (coding phosphoglucomutase, PGM(1) and PGM(2); glycerate dehydrogenase, GLYDH; phosphoglucose isomerase, PG1(2); glutamate dehydrogenase, GDH; glucose-6-phosphate dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-PGD(1); isocitrate dehydrogenase, IDH; diaphorase, DIA(2); malate dehydrogenase, MDH(1)) produce clear bands in seed tissue; however, glutamate oxaloacetate transaminase GOT(3) (N) was not found in seeds and shikimic dehydrogenase (SDH) could only be clearly resolved in needles (N). Several enzymes active in seed tissue could not be detected in needle tissues.Key words: Douglas-fir, needle tissue isozymes, inheritance.

scholarly journals Characterizing Isozymes of Spanish Cherimoya Cultivars

HortScience ◽  
1993 ◽  
Vol 28 (8) ◽  
pp. 845-847 ◽  
Author(s):  
L. Pascual ◽  
F. Perfectti ◽  
M. Gutierrez ◽  
A.M. Vargas
Keyword(s):  
Cluster Analysis ◽  
Superoxide Dismutase ◽  
Acid Phosphatase ◽  
Malic Enzyme ◽  
Leucine Aminopeptidase ◽  
Shikimate Dehydrogenase ◽  
Banding Patterns ◽  
Enzyme Systems ◽  
Annona Cherimola ◽  
Glutamate Oxalacetate Transaminase

Isozymes have been used as genetic markers to characterize seven Spanish cherimoya (Annona cherimola Mill.) cultivars. Fifteen enzyme systems were analyzed. Ten varied [aconitase (ACO, EC 4.2.1.3), alcohol dehydrogenase (ADH, EC 1.1.1.1), glutamate oxalacetate transaminase (GOT, EC 2.6.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), leucine aminopeptidase (LAP, EC 3.4.11.1), malate dehydrogenase (MDH, EC 1.1.1.37), phosphoglucose isomerase (PGI, EC 5.3.1.9), phosphoghtcomutase (PGM, EC 2.7.5.1), shikimate dehydrogenase (SKDH, EC 1.1.1.25), and triose phosphate isomerase (TPI, EC 5.3.1.1)] and five did not [acid phosphatase (ACPH, EC 3.1.3.2), diaphorase (DIA, EC 1.6.4.3), malic enzyme (ME, EC 1.1.1.40), 6-phosphogluconic dehydrogenase (6PGDH, EC 1.1.1.44), and superoxide dismutase (SOD, EC 1.15.1.1)]. Two cultivars, Campa and Campa Mejorada, had identical banding patterns for all enzymes tested. All others were identified as distinct cultivars because of isozyme differences. The identical isozyme profiles of `Campa' and `Campa Mejorada' probably indicate that they are the same cultivar. A cluster analysis of isozyme profiles showed that Spanish cultivars were clearly different from Californian cultivars.

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Measurement of Intrapopulational Enzyme Variation in Five Species of Epiphytic Lichens

1988 ◽  
Vol 20 (4) ◽  
pp. 377-384 ◽  
Author(s):  
Dianne Fahselt
Keyword(s):  
Alkaline Phosphatase ◽  
Superoxide Dismutase ◽  
Sexual Reproduction ◽  
Isoelectric Focusing ◽  
Evolutionary History ◽  
Enzyme System ◽  
Epiphytic Lichens ◽  
Enzyme Variation ◽  
Enzyme Systems ◽  
Individual Bands

AbstractThalli of five species of epiphytic lichens were collected from one moribund spruce-fir stand in the Algonquin Highlands in Ontario, Canada. Extracts of all were subjected to isoelectric focusing and stained for activity of 16 enzyme systems. Gels were scored for the presence or absence of individual bands in each enzyme system, and the degree of polymorphism of all detectable enzymes was evaluated using a variability measure developed for use with presence/absence data. Esterase and alkaline phosphatase showed the greatest amount of polymorphism and superoxide dismutase was the least variable enzyme system. The degree of enzyme variability in each of the five species was probably a reflection of its past evolutionary history and bore little relationship to apparent potential for sexual reproduction.

STUDY OF THE COMBINED INFLUENCE OF SODIUM THIOPENTAL AND DELTA SLEEP-INDUCING PEPTIDE ON ANTIOXIDANT DEFENSE SYSTEM IN RATS

2016 ◽  
pp. 49-52
Author(s):  
A. V. Shvetsov ◽  
E. G. Batotsyrenova ◽  
N. A. Dyuzhikova ◽  
V. A. Kashuro ◽  
N. V. Lapina ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Time Interval ◽  
Sodium Thiopental ◽  
Antioxidant Defense Enzymes ◽  
Delta Sleep ◽  
Thiopental Coma ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Delta Sleep Inducing Peptide

A biochemical investigation was performed into activity of rat antioxidant defense enzymes at different time interval after administration of sodium thiopental and delta-sleep-inducing peptide (DSIP). It was shown that thiopental coma was accompanied by a decreased level of superoxide dismutase ( 6 and 24 h after exposure) and increased level of caspase-3 ( 6 h after exposure) in the rat blood plasma. A pharmacological correction with DSIP induced a decrease of the level of superoxide dismutase ( 6 and 24 h after exposure), glutathione peroxidase and glucose 6-phosphate dehydrogenase (after 6h).

scholarly journals Pathogenicity and Virulence of Pythium Species Obtained from Forest Nursery Soils on Douglas-Fir Seedlings

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 744-748 ◽  
Author(s):  
Jerry E. Weiland ◽  
Bryan R. Beck ◽  
Anne Davis
Keyword(s):  
Pseudotsuga Menziesii ◽  
Seedling Survival ◽  
The United States ◽  
Douglas Fir ◽  
Tree Seedlings ◽  
Pythium Species ◽  
Forest Nursery ◽  
Damping Off ◽  
Greenhouse Study ◽  
Forest Nurseries

Pythium species are common soilborne oomycetes that occur in forest nursery soils throughout the United States. Numerous species have been described from nursery soils. However, with the exception of P. aphanidermatum, P. irregulare, P. sylvaticum, and P. ultimum, little is known about the potential for other Pythium species found in nursery soils to cause damping-off of tree seedlings. A greenhouse study was conducted to evaluate the pathogenicity and virulence of 44 Pythium isolates representing 16 species that were originally recovered from soil at three forest nurseries in Washington and Oregon. Seeds of Douglas-fir (Pseudotsuga menziesii) were planted into soil infested with each of the isolates. Seedling survival, the number of surviving seedlings with necrotic root lesions, and taproot length were evaluated 4 weeks later. Responses of Douglas-fir to inoculation varied significantly depending on Pythium species and isolate. Eight species (P. dissotocum, P. irregulare, P. aff. macrosporum, P. mamillatum, P. aff. oopapillum, P. rostratifingens, P. sylvaticum, and P. ultimum var. ultimum) significantly reduced the number of surviving seedlings compared to the noninoculated treatment. However, all Pythium species caused a greater percentage of seedlings to develop root lesions (total mean 40%) than was observed from noninoculated seedlings (17%). Taproot length varied little among Pythium treatments and was not a useful character for evaluating pathogenicity. Results confirm the ability of P. irregulare, P. mamillatum, and P. ultimum var. ultimum to cause damping-off of Douglas-fir seedlings, and are indicative that other species such as P. dissotocum, P. aff. macrosporum, P. aff. oopapillum, P. rostratifingens, and P. sylvaticum may also be responsible for seedling loss.

Cylindrocarpon and Fusarium root colonization of Douglas-fir seedlings from British Columbia reforestation sites

1998 ◽  
Vol 28 (8) ◽  
pp. 1198-1206 ◽  
Author(s):  
Paige E Axelrood ◽  
William K Chapman ◽  
Keith A Seifert ◽  
David B Trotter ◽  
Gwen Shrimpton
Keyword(s):  
British Columbia ◽  
Pseudotsuga Menziesii ◽  
Root Colonization ◽  
Linear Trend ◽  
Poor Performance ◽  
Douglas Fir ◽  
Root Infection ◽  
Significant Linear Trend ◽  
Conifer Seedlings ◽  
Cylindrocarpon Destructans

Poor performance of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) plantations established in 1987 has occurred in southwestern British Columbia. Affected sites were planted with 1-year-old container stock that exhibited some root dieback in the nursery. A study was initiated in 1991 to assess Cylindrocarpon and Fusarium root infection in planted and naturally regenerating (natural) Douglas-fir seedlings from seven affected plantations. Percentages of seedlings harboring Cylindrocarpon spp.and percent root colonization were significantly greater for planted seedlings compared with natural seedlings. A significant linear trend in Cylindrocarpon root colonization was observed for planted seedlings with colonization levels being highest for roots closest to the remnants of the root plug and decreasing at distances greater than 10cm from that region. This trend in Cylindrocarpon colonization was not observed for natural seedlings. Cylindrocarpon destructans (Zins.) Scholten var. destructans and C.cylindroides Wollenw. var. cylindroides were the only species isolated from planted and natural conifer seedlings. For most sites, percentage of seedlings harboring Fusarium spp.and percent Fusarium root colonization were less than for Cylindrocarpon. Recovery of Fusarium spp.from seedlings and root colonization levels were not significantly different for planted and natural seedlings from all sites.

scholarly journals Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides

1974 ◽  
Vol 137 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Inman ◽  
W. E. Hornby
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Inside Surface ◽  
Enzyme Systems ◽  
Nylon Tube ◽  
In Series ◽  
Automated Determination

1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and β-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising β-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.

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