ETUDE DE L'HEREDITE DE PHOSPHATASES ACIDES CHEZ LE DACTYLE (DACTYLIS GLOMERATA) DIPLOIDE ET TETRAPLOIDE

1981 ◽  
Vol 23 (3) ◽  
pp. 513-523 ◽  
Author(s):  
R. Lumaret

Starch gel electrophoresis of leaves of diploid and more particularly tetraploid orchard grass (Dactylis glomerata L.) from cultivars as well as natural populations disclosed several anodal stable bands with fast migration and phosphatasic activity. Six different phenotypes with one band, many others with three regular bands or six bands (only in the tetraploid individuals), were observed, showing the dimeric structure of the enzymes. Inheritance studies showed one polymorphic locus with six codominant alleles AcPH 11.00, AcPH 10.95, AcPH 10.90, AcPH 10.88, AcPH 11.02 and AcPH 11.04 with differences in enzymatic expression. AcPH 1.00 and AcPH 10.90 have been found in the two ploidy levels. Results from the tetraploid progenies involved tetravalents in meiosis for individuals originating from cultivars but bivalents in the wild plants. The two loci AcPH 1 and GOT 1 (this last one coding for a glutamate oxaloacetate transaminase) are not linked and seem to be located on different chromosomes.

1978 ◽  
Vol 68 (1) ◽  
pp. 85-97 ◽  
Author(s):  
S. J. Miles

AbstractThe genotypes of chromosomally-identified individuals from natural populations of the known species of the group of Anopheles gambiae Giles were scored for the enzyme protein structural loci coding for adenylate kinase (Adk), α-naphthyl acetate esterase (Est-1, Est-2, Est-3), glutamic-oxaloacetic transaminase (Got), α-glycerophosphate dehydrogenase (αGpd), hexokinase (Hk), isocitric dehydrogenase (Idh), lactic dehydrogenase (Ldh), ‘leucine’ aminopeptidase (Lap-2), malic enzyme (Me), octanol dehydrogenase (Odh), phosphoglucomutase (Pgm-1, Pgm-2), 6-phosphogluconic dehydrogenase (6-Pgd), phosphohexose isomerase (Phi) and superoxide dismutase (Sod), following starch gel electrophoresis. In the material examined, Est-1, Est-2, Est-3, Got, ldh, Lap-2, Odh, Pgm-1, Pgm-2 and Sod were segregating for two or more alleles; unique alleles at the Est-1, Got and Sod loci produced species-specific phenotypes in A. melas (Theo.), species C and species D, respectively. The further sampling of A. merus Dön, populations supported the presence of a unique SOD phenotype by which this species can also be identified. Of the other enzyme systems examined, no activity following electrophoresis was detected for aldolase and fructose-1,6-diphosphatase, and the resolution of acid and alkaline phosphatase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, malic dehydrogenase and xanthine dehydrogenase was too poor under the particular electrophoretic conditions for genetic analyses of the enzyme phenotypes.


1961 ◽  
Vol 41 (1) ◽  
pp. 211-216 ◽  
Author(s):  
J. T. Slykhuis

A number of cereals and other grasses were compared as hosts for different isolates of viruses causing Agropyron mosaic (AMV), wheat streak mosaic (WSMV), barley stripe mosaic (BSMV), ryegrass mosaic (RMV) and orchard grass mosaic (OMV). Lolium multiflorum L. was susceptible to all the viruses. The four varieties of wheat tested were highly susceptible to AMV, WSMV and BSMV, but not to RMV or OMV. Clintland oats was susceptible to WSMV, RMV and OMV but not to AMV or BSMV. Lolium perenne L. and Dactylis glomerata L. were infected by RMV and OMV only, Agropyron repens L. Beauv. by AMV only, and Setaria italica L. Beauv. by BSMV only. Brant and Husky barley were slightly susceptible to AMV, but seven other varieties appeared immune. Unusual host records include the infection of Setaria lutescens (Weigel) Hubb. and one plant of Agropyron smithii Rydb. with WSMV, and the infection of Agropyron intermedium (Host) Beauv. with BSMV. A list of differential hosts is proposed.


1972 ◽  
Vol 20 (1) ◽  
pp. 19-42 ◽  
Author(s):  
Francisco J. Ayala ◽  
Jeffrey R. Powell ◽  
Martin L. Tracey

SUMMARYWe have studied genetic variation at 27 loci in 42 samples from natural populations of a neotropical species, Drosophila equinoxialis, using standard techniques of starch-gel electrophoresis to detect allelic variation in genes coding for enzymes. There is considerarle genetic variability in D. equinoxialis. We have found allelic variation in each of the 27 loci, although not in every population. On the average, 71% of the loci are polymorphic – that is, the most common allele has a frequency no greater than 0·95 – in a given population. An individual is heterozygous on the average at 21·8% of its loci.The amount of genetic variation fluctuates widely from locus to locus. At the Mdh-2 locus arout 1% of the individuals are heterozygotes; at the other extreme more than 56% of the individuals are heterozygous at the Est-3. At any given locus the configuration of allelic frequencies is strikingly similar from locality to locality. At each and every locus the same allele is generally the most common throughout the distribution of the species. Yet differences in gene frequencies occur between localities. The pattern of genetic variation is incompatible with the hypothesis that the variation is adaptively neutral. Genetic variation in D. equinoxialis is maintained by balancing natural selection.The amount and pattern of genetic variation is similar in D. equinoxialis and its sibling species, D. willistoni. Yet the two species are genetically very different. Different sets of alleles occur at nearly 40% of the loci.


1992 ◽  
Vol 22 (12) ◽  
pp. 1838-1842 ◽  
Author(s):  
Catherine Zabinski

Tsugacanadensis (L.) Carr., commonly known as eastern hemlock, is a coniferous tree native to eastern North America. The western and southern edges of its geographic distribution are characterized by disjunct populations 50–100 km or more from the continuous distribution. Genetic variation was measured using starch gel electrophoresis of needle tissue enzymes from 17 populations throughout the species' distribution. Six enzyme systems including 10 putative loci were assayed, with only 1 of the 10 loci being polymorphic. The polymorphic locus, cytochrome oxidase, consisted of two alleles present in all but two populations. Allele frequencies averaged 0.69 and 0.31. The low level of genetic variation in eastern hemlock was an unexpected result, given that most coniferous species are highly variable.


1977 ◽  
Vol 57 (2) ◽  
pp. 615-616 ◽  
Author(s):  
M. R. HANNA ◽  
S. SMOLIAK ◽  
D. B. WILSON

Chinook is a winterhardy cultivar of orchard grass (Dactylis glomerata L.) developed by the late R. W. Peake at the Agriculture Canada Research Station, Lethbridge, Alberta. It was released in 1959, but a full description of this cultivar was not published.


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