scholarly journals THE GENETICS OF RESISTANCE TO PUCCINIA GRAMINIS TRITICI IN HEXAPLOID TRITICALE

1977 ◽  
Vol 19 (4) ◽  
pp. 683-693 ◽  
Author(s):  
R. J. Morrison ◽  
E. N. Larter ◽  
G. J. Green
Keyword(s):  
Resistance Genes ◽  
Single Gene ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Genetics Of Resistance ◽  
Hexaploid Triticale ◽  
Wheat Stem Rust ◽  
Seedling Resistance ◽  
Plant Stage ◽  
Triticosecale Wittmack

The inheritance of resistance to wheat stem rust, Puccinia gaminis tritici, was studied in five hexaploid triticales (× Triticosecale Wittmack) using races C33 (15B-IL) and C17(56). Triticale lines 70HN458 and 6TA204 each possessed a single gene for seedling resistance; Rosner and MT 36-1 each carried two genes, and 6A190, three. The cultivars carried a total of at least five different dominant resistance genes. Four of these genes conditioned resistance to both rust races (C17 and C33) while a gene from line 6A190 conditioned resistance to a single race (C17 only). The seedling resistance genes also conferred resistance to adult plants while genes conferring resistance only in the adult plant stage were not detected.

scholarly journals Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10580
Author(s):  
Qiujun Lin ◽  
Yue Gao ◽  
Xianxin Wu ◽  
Xinyu Ni ◽  
Rongzhen Chen ◽  
...  
Keyword(s):  
Stem Rust ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Heilongjiang Province ◽  
Wheat Stem Rust ◽  
Breeding Programs ◽  
Plant Stage ◽  
Molecular Marker Analysis ◽  
Wheat Lines ◽  
New Germplasm

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spread of the Ug99 race variants. Therefore, there is an urgent global effort to identify new germplasm resources effective against those races. In this study, the resistance to Pgt of 95 wheat advance lines from Heilongjiang Province was evaluated using three predominant races of Pgt, 21C3CTTTM, 34C0MKGSM, and 34C3MTGQM, in China at the seedling and adult plant stage. The presence of 6 Sr genes (Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38) was evaluated using linked molecular markers. The results showed that 86 (90.5%) wheat lines had plant stage resistance to all three races. Molecular marker analysis showed that 24 wheat lines likely carried Sr38, 15 wheat lines likely carried Sr2, 11 wheat lines likely carried Sr31, while none of the wheat lines carried Sr24, Sr25, or Sr26. Furthermore, six out of the 95 wheat lines tested carried both Sr2 and Sr38, three contained both Sr31 and Sr38, and two wheat lines contained both Sr2 and Sr31. Wheat lines with known Sr genes may be used as donor parents for further breeding programs to provide resistance to stem rust.

scholarly journals Emergence of Virulence to Sr25 of Puccinia graminis f. sp. tritici on Wheat in India

Plant Disease ◽  
2009 ◽  
Vol 93 (8) ◽  
pp. 840-840 ◽  
Author(s):  
S. K. Jain ◽  
M. Prashar ◽  
S. C. Bhardwaj ◽  
S. B. Singh ◽  
Y. P. Sharma
Keyword(s):  
Resistance Genes ◽  
Stem Rust ◽  
Infection Type ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Wheat Cultivars ◽  
Sources Of Resistance ◽  
Seedling Resistance ◽  
Additional Gene ◽  
Adult Plants

Stem (black) rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks. & Henn., is one of the most destructive diseases of wheat. It could be controlled through introgression of race-specific resistance genes. However, such kind of resistance is mostly short lived due to emergence of new virulences. For example, resistance genes Sr11, Sr24, Sr30, and Sr31 are no longer effective (2,4). Detection of new virulences has remained vital in the evaluation and identification of new sources of resistance. We report here the detection of virulence to Sr25, a gene from Thinopyrum elongatum (4), which had been effective or partially effective against stem rust worldwide, including race Ug99 (TTKSK) (4). A stem rust isolate collected in 2006 from Karnataka (southern India) produced susceptible reactions (infection type [IT] 3+ to 4) on the primary leaves of differential genotype ‘Agatha’ carrying Sr25 and susceptible check ‘Agra Local’ at 22 ± 2°C. To verify virulence to Sr25, single-pustule isolates from this sample were inoculated onto seedlings of ‘Agrus’, ‘Agatha’, ‘RL6040’ (‘Thatcher’ + Sr25), ‘Superseri#1’, ‘Wheatear’, and ‘Morocco’ + Sr25 (obtained from CIMMYT), which all carry Sr25. All these accessions were found susceptible (IT 3+ to 4) to this isolate, except Wheatear which expressed resistance (IT ;1), indicating the presence of additional gene(s). These genotypes are resistant (ITs ;1 to 2+) to Sr25-avirulent pathotypes. The new pathotype is avirulent to Sr11, 13, 14, 21, 22, 23, 24, 26, 27, 29, 31, 32, 33, 35, 37, 38, 39, 40, 43, and Tmp and virulent to Sr5, 6, 7a, 7b, 8a, 9a, 9b, 9d, 9e, 9f, 9g, 10, 12, 15, 16, 17, 18, 19, 20, 25, 28, 30, 34, 36, 42, Wld-1, and Gt at 22 ± 2°C. This pathotype has been designated as 58G13-3 and PKTSC according to the Indian nomenclature (1) and the North American system (3), respectively. It represents race 40 based on Stakman's differentials. It may have arisen from race 40 through mutation. The type culture of the pathotype has been added to the culture collection at Flowerdale, Shimla. Interestingly, ‘Festiguay’ (Sr30) was found resistant to this pathotype, indicating the presence of additional gene(s), whereas ‘Webster’ (Sr30) was susceptible. Adult plants of Agrus, Agatha, RL6040, Superseri#1, and Morocco+Sr25 also were susceptible, producing 20S to 60S responses. Sr25-avirulent pathotype 62G29 produced a TR (flecking in traces) response on these lines except Morocco + Sr25 that showed 20 to 40MR (moderately resistant) responses. In the same study however, adult plants of Thatcher showed a resistant reaction (10R to MR) at low (16 ± 2°C) and susceptible (20S) at high (22 ± 2°C) temperatures. Agatha and RL6040, having Thatcher as one of the parents, had similar responses. The detection of Sr25 virulence is significant since Sr25 is an important gene to be targeted for breeding wheat cultivars resistant to Ug99. We should use either adult plant resistance and/or pyramiding two or more genes for seedling resistance to enhance the field life of wheat cultivars. References: (1) P. Bahadur et al. Proc. Indian Acad. Sci. 95:29, 1985. (2) S. C. Bhardwaj et al. J. Wheat Res. 1:51, 2007. (3) Y. Jin et al. Plant Dis. 92:923, 2008. (4) R. P. Singh et al. CAB Rev. No. 054:1, 2006.

scholarly journals Genetic Analysis of Leaf Rust Resistance in Six Durum Wheat Genotypes

2014 ◽  
Vol 104 (12) ◽  
pp. 1322-1328 ◽  
Author(s):  
Alexander Loladze ◽  
Dhouha Kthiri ◽  
Curtis Pozniak ◽  
Karim Ammar
Keyword(s):  
Durum Wheat ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Allelism Tests

Leaf rust, caused by Puccinia triticina, is one of the main fungal diseases limiting durum wheat production. This study aimed to characterize previously undescribed genes for leaf rust resistance in durum wheat. Six different resistant durum genotypes were crossed to two susceptible International Maize and Wheat Improvement Center (CIMMYT) lines and the resulting F1, F2, and F3 progenies were evaluated for leaf rust reactions in the field and under greenhouse conditions. In addition, allelism tests were conducted. The results of the study indicated that most genotypes carried single effective dominant or recessive seedling resistance genes; the only exception to this was genotype Gaza, which carried one adult plant and one seedling resistance gene. In addition, it was concluded that the resistance genes identified in the current study were neither allelic to LrCamayo or Lr61, nor were they related to Lr3 or Lr14a, the genes that already are either ineffective or are considered to be vulnerable for breeding purposes. A complicated allelic or linkage relationship between the identified genes is discussed. The results of the study will be useful for breeding for durable resistance by creating polygenic complexes.

scholarly journals Characterization of Wheat Monogenic Lines with Known Sr Genes and Wheat Lines with Resistance to the Ug99 Race Group for Resistance to Prevalent Races of Puccinia graminis f. sp. tritici in China

Plant Disease ◽  
2020 ◽  
Vol 104 (7) ◽  
pp. 1939-1943
Author(s):  
Xian Xin Wu ◽  
Qiu Jun Lin ◽  
Xin Yu Ni ◽  
Qian Sun ◽  
Rong Zhen Chen ◽  
...  
Keyword(s):  
Food Security ◽  
Stem Rust ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Wheat Cultivars ◽  
Wheat Stem Rust ◽  
Wheat Improvement ◽  
Wheat Lines ◽  
Sr Genes

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is one of the most serious fungal diseases in wheat production, seriously threatening the global supply of wheat and endangering food security. The present study was conducted to evaluate wheat monogenic lines with known Sr genes to the most prevalent P. graminis f. sp. tritici races in China. In addition, wheat lines introduced from the International Maize and Wheat improvement Center (CIMMYT) with resistance to the Ug99 race group were also evaluated with the prevalent Chinese P. graminis f. sp. tritici races. The monogenic lines containing Sr9e, Sr21, Sr26, Sr31, Sr33, Sr35, Sr37, Sr38, Sr47, and SrTt3 were effective against races 21C3CTTTM, 34C0MRGSM, and 34C3MTGQM at both seedling and adult-plant stages. In contrast, monogenic lines containing Sr6, Sr7b, Sr8a, Sr9a, Sr9b, Sr9d, Sr9f, Sr9g, Sr13, Sr16, Sr18, Sr19, Sr20, Sr24, Sr28, Sr29, and Sr34 were highly susceptible to these races at both seedling and adult-plant stages. Lines with Sr5, Sr10, Sr13, Sr14, Sr15, Sr17, Sr21, Sr22, Sr23, Sr25, Sr27, Sr29, Sr30, Sr32, Sr36, and Sr39 were resistant to one or more of the tested races. Among the 123 CIMMYT lines, 38 (30.9%) showed varying levels of susceptibility to Chinese P. graminis f. sp. tritici races. The results should be useful for breeding wheat cultivars with resistance to stem rust.

Inheritance of resistance to stem rust in 'Bonza', 'Chris', 'FKN-II-50-17', 'MRFY', 'Thatcher', 'Marquillo', and 'Hope' wheats

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 269-276
Author(s):  
M. Padidam ◽  
D. R. Knott
Keyword(s):  
Triticum Aestivum ◽  
Plant Resistance ◽  
Stem Rust ◽  
Adult Plant Resistance ◽  
Major Gene ◽  
Triticum Aestivum L ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Seedling Resistance ◽  
Minor Genes

Resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks, and Henn.), particularly adult plant resisitance to race 15B-1, was studied in seven wheat (Triticum aestivum L.) cultivars or lines: 'Bonza', 'Chris', 'FKN-II-50-17', 'MRFY', 'Thatcher', 'Marquillo', and 'Hope'. Each of the seven was crossed with a susceptible parent and either F4- or F5-derived lines developed by single seed descent. All of the lines were tested with race 15B-1 in field nurseries. Lines derived from parents carrying seedling resistance to race 15B-1 were also tested as seedlings in the greenhouse with race 15B-1, and in some cases races 56, 29, and C65. The data indicated that 'Bonza' carries Sr6, probably Sr5, an unidentified gene giving resistance to race 56, two unidentified genes for resistance to race C65, and two minor genes that combine to produce intermediate adult plant resistance. 'Chris' carries Sr5, Sr7a, Sr8a, and Sr12. In addition, it may have three minor genes for adult plant resistance. 'FKN-II-50-17' carries Sr6 and may have four minor genes that combine to produce moderate adult plant resistance. 'MRFY', which is seedling susceptible to race 15B-1, carries Sr9b, possibly Sr5, plus an unidentified gene for resistance to C65. In addition, it appears to have one major gene for adult plant resistance plus two or more minor genes. 'Thatcher', 'Marquillo', and 'Hope' had only limited resistance to race 15B-1 in the field and no genetic analysis of their crosses was possible. The four parents that had good resistance to race 15B-1 in the field, 'Bonza', 'Chris', 'FKN-II-50-17', and 'MRFY', all carry minor genes for adult plant resistance that had little effect individually but produced moderate resistance when combined. The genes Sr5 and Sr9b, which have no effect on resistance to 15B-1 is seedlings, were found to significantly increase resistance in adult plants in the field.Key words: stem rust, Puccinia graminis tritici, wheat, Triticum aestivum, adult plant rust resistance.

scholarly journals Analyzing Genetic Diversity for Virulence and Resistance Phenotypes in Populations of Stem Rust (Puccinia graminis f. sp. secalis) and Winter Rye (Secale cereale)

2016 ◽  
Vol 106 (11) ◽  
pp. 1335-1343 ◽  
Author(s):  
Thomas Miedaner ◽  
Ann-Kristin Schmitt ◽  
Bettina Klocke ◽  
Brigitta Schmiedchen ◽  
Peer Wilde ◽  
...  
Keyword(s):  
Stem Rust ◽  
Resistance Breeding ◽  
The United States ◽  
Shannon Index ◽  
Adult Plant ◽  
Leaf Segment ◽  
Winter Rye ◽  
Puccinia Graminis ◽  
Plant Stage ◽  
Rust Severity

Stem rust (Puccinia graminis f. sp. secalis) leads to considerable yield losses in rye-growing areas with continental climate, from Eastern Germany to Siberia. For implementing resistance breeding, it is of utmost importance to (i) analyze the diversity of stem rust populations in terms of pathotypes (= virulence combinations) and (ii) identify resistance sources in winter rye populations. We analyzed 323 single-uredinial isolates mainly collected from German rye-growing areas across 3 years for their avirulence/virulence on 15 rye inbred differentials. Out of these, 226 pathotypes were detected and only 56 pathotypes occurred more than once. This high diversity was confirmed by a Simpson index of 1.0, a high Shannon index (5.27), and an evenness index of 0.97. In parallel, we investigated stem rust resistance among and within 121 heterogeneous rye populations originating mainly from Russia, Poland, Austria, and the United States across 3 to 15 environments (location−year combinations). While German rye populations had an average stem rust severity of 49.7%, 23 nonadapted populations were significantly (P < 0.01) more resistant with a stem rust severity ranging from 3 to 40%. Out of these, two modern Russian breeding populations and two old Austrian landraces were the best harboring 32 to 70% fully resistant plants across 8 to 10 environments. These populations with the lowest disease severity in adult-plant stage in the field also displayed resistance in leaf segment tests. In conclusion, stem rust populations are highly diverse and the majority of resistances in rye populations seems to be race specific.

scholarly journals Mapping and Characterization of a Wheat Stem Rust Resistance Gene in Durum Wheat “Kronos”

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongna Li ◽  
Lei Hua ◽  
Matthew N. Rouse ◽  
Tianya Li ◽  
Shuyong Pang ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Wheat Variety ◽  
Wheat Breeding ◽  
Stem Rust Resistance ◽  
Wheat Stem Rust ◽  
Seedling Resistance ◽  
Or Gene

Wheat stem (or black) rust is one of the most devastating fungal diseases, threatening global wheat production. Identification, mapping, and deployment of effective resistance genes are critical to addressing this challenge. In this study, we mapped and characterized one stem rust resistance (Sr) gene from the tetraploid durum wheat variety Kronos (temporary designation SrKN). This gene was mapped on the long arm of chromosome 2B and confers resistance to multiple virulent Pgt races, such as TRTTF and BCCBC. Using a large mapping population (3,366 gametes), we mapped SrKN within a 0.29 cM region flanked by the sequenced-based markers pku4856F2R2 and pku4917F3R3, which corresponds to 5.6- and 7.2-Mb regions in the Svevo and Chinese Spring reference genomes, respectively. Both regions include a cluster of nucleotide binding leucine-repeat (NLR) genes that likely includes the candidate gene. An allelism test failed to detect recombination between SrKN and the previously mapped Sr9e gene. This result, together with the similar seedling resistance responses and resistance profiles, suggested that SrKN and Sr9e may represent the same gene. We introgressed SrKN into common wheat and developed completely linked markers to accelerate its deployment in the wheat breeding programs. SrKN can be a valuable component of transgenic cassettes or gene pyramids that includes multiple resistance genes to control this devastating disease.

scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

scholarly journals Pathogenic Variability of Wheat Stem Rust Pathogen (Puccinia graminis f. sp. tritici) in Hararghe Highlands, Ethiopia

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Arif Abrahim ◽  
Temam Hussein ◽  
Ayele Badebo
Keyword(s):  
Resistance Genes ◽  
Stem Rust ◽  
Agricultural Research ◽  
Low Frequency ◽  
Puccinia Graminis ◽  
Total Production ◽  
Rust Disease ◽  
Wheat Stem Rust ◽  
Pathogenic Variability ◽  
Infection Types

Wheat is one of the important major crops of Hararghe Highlands. It is third in land coverage and total production after sorghum and maize. However, the wheat stem rust disease is threatening production of wheat in this region. So, this research was conducted with the following objective: to determine the population of Puccinia graminis f. sp. tritici in Hararghe Highlands. A total of 200 fields were surveyed and stem rust samples were collected and transported to Kulumsa Agricultural Research Center for race analysis. Inoculation of differentials carrying resistance genes Sr24 and Sr-Tmp indicated typical low infection types on all isolates. Isolates EH5, EH8, and EH3 from East Hararghe and WH2, WH1, and WH3 from West Hararghe showed high virulence of infection in all differential lines. Ten (10) races were identified by using Puccinia graminis tritici code system: TTGSK, PTJQK, TTSSK, TTKSK, TRSSK, and TTJQK from East Hararghe and TTTSK and TTSQK from West Hararghe zones. Race TTSSK was most frequent (25%) followed by TTKSK (25%) in East Hararghe. Race TTSSK showed 50% frequency of occurrence in West Hararghe zones. The low frequency of occurrences indicated high variability of the races in the survey areas. Therefore, monitoring of populations of pathogens is important for the national and regional research centers. Detection of pathogen virulence evolution and of currently effective resistance genes is necessary and must be applied within a system of resistance gene management.

Physiologic races of wheat stem rust in Canada from 1919 to 1969

1971 ◽  
Vol 49 (9) ◽  
pp. 1575-1588 ◽  
Author(s):  
G. J. Green
Keyword(s):  
Resistance Genes ◽  
Stem Rust ◽  
Single Gene ◽  
Effective Resistance ◽  
Stabilizing Selection ◽  
New Combinations ◽  
Wheat Stem Rust ◽  
The Past ◽  
Physiologic Races ◽  
New System

The races of wheat stem rust found in Canada and identified by the "standard" and "formula" methods are described. Seven races or race groups made up 90.8% of the isolates. In 1964 a new system of race identification was introduced. It makes use of virulence formulas consisting of the numbers of effective resistance genes in the numerator and ineffective genes in the denominator. Resistance genes Sr6, Sr8, Sr9a, Sr9b, and Sr13 confer resistance to most isolates obtained since 1964, but no single gene confers resistance to all isolates. Experience shows that 250 isolates per year are sufficient to establish the trends of prevalence of the main races. The detection of rare, new combinations of virulence requires screening varieties carrying combinations of resistance genes. The main races in the past 50 years have been 56 and 15B. Race 56 is uniform with a single subrace but 15B is variable with many subraces. There is no evidence that unnecessary virulence is harmful or that "stabilizing selection" is operative in Canada, nor is there evidence that virulence on resistance genes Sr6 and Sr11 is harmful to the rust.

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