MEIOSIS IN 8-CHROMOSOME BARLEY HAPLOIDS

1977 ◽  
Vol 19 (2) ◽  
pp. 211-216 ◽  
Author(s):  
George Fedak
Keyword(s):  
Hordeum Vulgare ◽  
Extra Chromosome ◽  
Normal Chromosome ◽  
Short Chromosome ◽  
Hordeum Vulgare L ◽  
Normal Complement ◽  
Chromosome Synapsis ◽  
Haploid Plants

Meiosis was studied in four 8-chromosome haploid plants produced from Wiebe's barley (2n = 14 + 2 centric fragments) (Hordeum vulgare L.) in which the extra chromosome had lost its telomeres and was one-third the length of a normal chromosome. Although secondary associations occurred, pairing between the normal and fragmented chromosome was rare despite the presence of homologous segments between them, indicating that the deletion of the telomeres restricted chromosome synapsis. A high bivalent frequency was attributed to a translocation between a long and short chromosome of the normal complement that persisted through the haploidization process.

Influence of Fe concentration in the medium on multicellular pollen grains and haploid plants induced by mannitol pretreatment in barley (Hordeum vulgare L.)

PROTOPLASMA ◽  
2006 ◽  
Vol 228 (1-3) ◽  
pp. 101-106 ◽  
Author(s):  
A. Pulido ◽  
F. Bakos ◽  
A. Castillo ◽  
M. P. Vallés ◽  
B. Barnabás ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Pollen Grains ◽  
Hordeum Vulgare L ◽  
Haploid Plants

Transformation of isolated barley (Hordeum vulgare L.) microspores: II. Timing of pretreatment and temperatures relative to results of bombardment

Genome ◽  
2009 ◽  
Vol 52 (2) ◽  
pp. 175-190 ◽  
Author(s):  
Youn-Seb Shim ◽  
K. Peter Pauls ◽  
Ken J. Kasha
Keyword(s):  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Culture Medium ◽  
Doubled Haploids ◽  
Arabinogalactan Protein ◽  
35S Promoter ◽  
Hordeum Vulgare L ◽  
Actin Promoter ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Based on paper I in this series, our goals in this paper were to determine the relationship between prebombardment pretreatments and temperatures, microspore cell cycle when bombarded, and the frequencies of homozygous and hemizygous transgenic progeny in barley ( Hordeum vulgare L.). Of the 104 fluorescent plants selected when using the GFP fluorescence transgene, 28 were albino and 76 plants were green. Thirty-one green plants were confirmed to be transgenic; the others were either transient green fluorescent protein expression or selected due to autofluorescence. Of the 31 plants, 23 came from embryos expressing a high level of fluorescence during selection and eight from 51 plants exhibiting a low level of fluorescence. Of the two pretreatments used to induce embryogenesis, 24 of 31 plants were from the cold pretreatment for 21 days (C) versus seven from the 4 day cold plus mannitol pretreatment. Following pretreatment, the microspores were subjected to a high-osmotic period (0.5 mol/L mannitol plus sorbitol) of 4 h prebombardment and 18 h postbombardment at either 25 or 4 °C. Of the 31 transgenic plants, 19 were produced following the 25 °C 4 h prebombardment. Sixteen of the 19 were doubled haploid plants (seven being homozygous for the transgene) and the other three plants were haploid. Of the remaining 12 plants recovered following the 4 h 4 °C prebombardment treatment, nine were haploid and three were doubled haploid plants, two of the latter being homozygous for the transgene. All 12 haploid plants obtained were treated with colchicine and produced homozygous transgenic doubled haploids. Of the two promoters compared, 30 plants had the actin promoter and only one had the 35S promoter. The use of arabinogalactan protein in the culture medium was very beneficial, giving rise to 29 of the 31 plants. The best procedure for obtaining transgenic barley plants from this study was pretreatment C, leaving the cultures at either 4 or 25 °C during the 4 h prebombardment high-osmotic period, using the actin promoter and having arabinogalactan protein in the microspore culture medium. With this procedure, the transgenic frequency was improved 8- to10-fold over previous reports on bombardment of microspores. It yielded about one transgenic plant per Petri dish and is comparable with Agrobacterium frequencies on structures derived from microspores.

EFFECT OF GAMMA RAYS AND SOME CHEMICAL MUTAGENS ON INDUCTION OF TELOTRISOMICS IN HORDEUM

1973 ◽  
Vol 15 (4) ◽  
pp. 815-824 ◽  
Author(s):  
H. I. Sayed ◽  
S. B. Helgason ◽  
E. N. Larter
Keyword(s):  
Hordeum Vulgare ◽  
Gamma Rays ◽  
Extra Chromosome ◽  
Chromosome 7 ◽  
Adjacent Region ◽  
Hordeum Vulgare L ◽  
Chemical Mutagens ◽  
Chromosome Breaks ◽  
Γ Rays ◽  
High Degree

Trisomic lines for each of chromosomes 1,3,4,5, and 7 of barley (Hordeum vulgare L.) were subjected to mutagens viz, EMS, DES, HA, FUdR and γ rays in an attempt to induce telocentrics in the extra chromosome. DES and EMS caused a relatively high degree of lethality of trisomies compared with that of disomics. Treatment with HA and γ rays at the time of meiosis caused almost complete sterility. No telotrisomics were recovered from these treatments. Irradiation of trisomic seedlings resulted in the isolation of 11 fragments of which 6 were true telocentrics. The frequency of telocentrics was significantly increased over non-treated material only in stocks of trisomic for chromosomes 1 and 7. All chromosome breaks induced in chromosome 7 were localized at the centromere and the adjacent region of the long arm.

Ultrastructure and Senescence in an Achloroplastic Mutant of Hordeum vulgare L. cv. Dyan

1992 ◽  
Vol 50 (1) ◽  
pp. 844-845
Author(s):  
R.H.M. Cross ◽  
C.E.J. Botha ◽  
A.K. Cowan ◽  
B.J. Hartley
Keyword(s):  
Cell Wall ◽  
Hordeum Vulgare ◽  
Leaf Tissue ◽  
Ultrastructural Changes ◽  
Hordeum Vulgare L ◽  
Mesophyll Cells ◽  
Lethal Mutant ◽  
Cytoplasmic Matrix ◽  
Close Proximity ◽  
Pinocytotic Vesicles

Senescence is an ordered degenerative process leading to death of individual cells, organs and organisms. The detection of a conditional lethal mutant (achloroplastic) of Hordeum vulgare has enabled us to investigate ultrastructural changes occurring in leaf tissue during foliar senescence.Examination of the tonoplast structure in six and 14 day-old mutant tissue revealed a progressive degeneration and disappearance of the membrane, apparently starting by day six in the vicinity of the mitochondria associated with the degenerating proplastid (Fig. 1.) where neither of the plastid membrane leaflets is evident (arrows, Fig. 1.). At this stage there was evidence that the mitochondrial membranes were undergoing retrogressive changes, coupled with disorganization of cristae (Fig. 2.). Proplastids (P) lack definitive prolamellar bodies. The cytoplasmic matrix is largely agranular, with few endoplasmic reticulum (ER) cisternae or polyribosomal aggregates. Interestingly, large numbers of actively-budding dictysomes, associated with pinocytotic vesicles, were observed in close proximity to the plasmalemma of mesophyll cells (Fig. 3.). By day 14 however, mesophyll cells showed almost complete breakdown of subcellular organelle structure (Fig. 4.), and further evidence for the breakdown of the tonoplast. The final stage of senescence is characterized by the solubilization of the cell wall due to expression and activity of polygalacturonase and/or cellulose. The presence of dictyosomes with associated pinocytotic vesicles formed from the mature face, in close proximity to both the plasmalemma and the cell wall, would appear to support the model proposed by Christopherson for the secretion of cellulase. This pathway of synthesis is typical for secretory glycoproteins.

ИЗМЕНЧИВОСТЬ ЯЧМЕНЯ (Hordeum vulgare L.) РАЗНОГО ГЕОГРАФИЧЕСКОГО ПРОИСХОЖДЕНИЯ ПО ЭЛЕМЕНТАМ СТРУКТУРЫ УРОЖАЯ

2012 ◽  
pp. 33-40
Author(s):  
А.В. ЖЕЛЕЗНОВ ◽  
◽  
Н.Б. ЖЕЛЕЗНОВА ◽  
Т.В. КУКОЕВА ◽  
Н.В. БУРМАКИНА ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L

ВНУТРИВИДОВОЙ ПОЛИМОРФИЗМ ЯРОВОГО ЯЧМЕНЯ (Hordeum vulgare L.) ПО УСТОЙЧИВОСТИ К ДЕЙСТВИЮ СВИНЦА

2014 ◽  
pp. 78-87 ◽  
Author(s):  
А.В. ДИКАРЕВ ◽  
◽  
В.Г. ДИКАРЕВ ◽  
Н.С. ДИКАРЕВА ◽  
С.А. ГЕРАСЬКИН ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L

Using AMMI approach to delineate genotype by environment interaction and stability of barley (Hordeum vulgare L.) genotypes under northern Indian shivalik hill conditions

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Om Prakash Yadav ◽  
A. K. Razdan ◽  
Bupesh Kumar ◽  
Praveen Singh ◽  
Anjani K. Singh
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Principal Component ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Hordeum Vulgare L ◽  
Genotype By Environment ◽  
Biplot Analysis ◽  
Principal Component Axis ◽  
Ammi Analysis

Genotype by environment interaction (GEI) of 18 barley varieties was assessed during two successive rabi crop seasons so as to identify high yielding and stable barley varieties. AMMI analysis showed that genotypes (G), environment (E) and GEI accounted for 1672.35, 78.25 and 20.51 of total variance, respectively. Partitioning of sum of squares due to GEI revealed significance of interaction principal component axis IPCA1 only On the basis of AMMI biplot analysis DWRB 137 (41.03qha–1), RD 2715 (32.54qha–1), BH 902 (37.53qha–1) and RD 2907 (33.29qha–1) exhibited grain yield superiority of 64.45, 30.42, 50.42 and 33.42 per cent, respectively over farmers’ recycled variety (24.43qha–1).

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