BANDING ANALYSIS OF THE SOMATIC CHROMOSOMES OF THE DOMESTIC DOG (CANIS FAMILIARIS)

1976 ◽  
Vol 18 (3) ◽  
pp. 513-518 ◽  
Author(s):  
M. Manolache ◽  
W. M. Ross ◽  
M. Schmid
Keyword(s):  
Constitutive Heterochromatin ◽  
Canis Familiaris ◽  
The Other ◽  
Domestic Dog ◽  
Hoechst 33258 ◽  
Banding Analysis ◽  
Staining Techniques

The chromosomes of the domestic dog (Beagle) were investigated by several different staining techniques. G-banding, Q-banding, and the bis-benzimidazol derivative Hoechst 33258, make possible the identification of all 39 chromosome pairs. Constitutive heterochromatin (C-bands) was present on a few chromosomes as distinctive, large stained areas; on the other autosomes there was little or no heterochromatin detectable.

Characterization and modification of heterochromatin in four species of the immigrans species group of Drosophila

1986 ◽  
Vol 28 (3) ◽  
pp. 340-347 ◽  
Author(s):  
J. P. Gupta ◽  
Arun Kumar
Keyword(s):  
Significant Role ◽  
Constitutive Heterochromatin ◽  
Species Group ◽  
Hoechst 33258 ◽  
Mitotic Chromosomes ◽  
Fluorescence Staining ◽  
Base Pairs ◽  
C Banding ◽  
Bright Fluorescence ◽  
Staining Techniques

The distribution of constitutive heterochromatin in mitotic chromosomes of four species (viz., Drosophila quadrilineata de Meijere, D. immigrans Sturtevant, D. pulaua Wheeler, and D. kohkoa Wheeler) was studied using C-banding and fluorescence staining techniques. The results of this study had revealed that the heterochromatic segments detected by C-banding in the species under study were found to coincide precisely with the areas giving bright fluorescence with the two fluorochromes, Hoechst 33258 and quinacrine. This suggested the presence of A–T rich base pairs in their heterochromatin. These studies further revealed that the modification of heterochromatin caused due to the additions or deletions in particular had also played a very significant role during the differentiation of these species.Key words: heterochromatin modification, immigrans species group, Drosophila.

Trypanosoma caninum n. sp. (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil

Parasitology ◽  
2009 ◽  
Vol 136 (4) ◽  
pp. 411-423 ◽  
Author(s):  
M. F. MADEIRA ◽  
M. A. SOUSA ◽  
J. H. S. BARROS ◽  
F. B. FIGUEIREDO ◽  
A. FAGUNDES ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Canis Familiaris ◽  
Axenic Culture ◽  
The Other ◽  
Domestic Dog ◽  
Intact Skin ◽  
Specific Primers ◽  
Isoenzyme Analysis ◽  
Triatomine Bugs ◽  
Pcr Assays

SUMMARYAn unknown Trypanosoma species was isolated from an axenic culture of intact skin from a domestic dog captured in Rio de Janeiro, Brazil, which was co-infected with Leishmania (Viannia) braziliensis. Giemsa-stained smears of cultures grown in different media revealed the presence of epimastigotes, trypomastigotes, spheromastigotes, transitional stages, and dividing forms (epimastigotes or spheromastigotes). The highest frequency of trypomastigotes was observed in RPMI (15·2%) and DMEM (9·2%) media containing 5% FCS, with a mean length of these forms of 43·0 and 36·0 μm, respectively. Molecular analysis by sequential application of PCR assays indicated that this trypanosome differs from Trypanosoma cruzi and T. rangeli when specific primers were applied. On the other hand, a PCR strategy targeted to the D7 domain of 24sα rDNA, using primers D75/D76, amplified products of about 250 bp in that isolate (stock A-27), different from the amplification products obtained with T. cruzi and T. rangeli. This organism differs from T. cruzi mainly by the size of its trypomastigote forms and kinetoplasts and the absence of infectivity for macrophages and triatomine bugs. It is also morphologically distinct from salivarian trypanosomes reported in Brazil. Isoenzyme analysis at 8 loci demonstrated a very peculiar banding pattern clearly distinct from those of T. rangeli and T. cruzi. We conclude that this isolate is a new Trypanosoma species. The name T. caninum is suggested.

Associations among behavioral inhibition and owner-rated attention, hyperactivity/impulsivity, and personality in the domestic dog (Canis familiaris).

2019 ◽  
Vol 133 (2) ◽  
pp. 233-243 ◽  
Author(s):  
Nóra Bunford ◽  
Barbara Csibra ◽  
Csenge Peták ◽  
Bence Ferdinandy ◽  
Ádám Miklósi ◽  
...  
Keyword(s):  
Behavioral Inhibition ◽  
Canis Familiaris ◽  
Domestic Dog

Diversity and Karyotypic Evolution in the Genus Neacomys (Rodentia, Sigmodontinae)

2015 ◽  
Vol 146 (4) ◽  
pp. 296-305 ◽  
Author(s):  
Willam O. da Silva ◽  
Julio C. Pieczarka ◽  
Rogério V. Rossi ◽  
Horacio Schneider ◽  
Iracilda Sampaio ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Diploid Number ◽  
Constitutive Heterochromatin ◽  
Chromosomal Evolution ◽  
The Other ◽  
Karyotypic Evolution ◽  
Drastic Reduction ◽  
Ancestral Species ◽  
Pericentric Inversions ◽  
Amazonian Region

Neacomys (Sigmodontinae) comprises 8 species mainly found in the Amazonian region. We describe 5 new karyotypes from Brazilian Amazonia: 2 cytotypes for N. paracou (2n = 56/FNa = 62-66), 1 for N. dubosti (2n = 64/FNa = 68), and 2 for Neacomys sp. (2n = 58/FNa = 64-70), with differences in the 18S rDNA. Telomeric probes did not show ITS. We provide a phylogeny using Cytb, and the analysis suggests that 2n = 56 with a high FNa is ancestral for the genus, as found in N. paracou, being retained by the ancestral forms of the other species, with an increase in 2n occurring independently in N. spinosus and N. dubosti. Alternatively, an increase in 2n may have occurred in the ancestral taxon of the other species, followed by independent 2n-reduction events in Neacomys sp. and in the ancestral species of N. tenuipes, N. guianae, N. musseri, and N. minutus. Finally, a drastic reduction event in the diploid number occurred in the ancestral species of N. musseri and N. minutus which exhibit the lowest 2n of the genus. The karyotypic variations found in both intra- and interspecific samples, associated with the molecular phylogeny, suggest a chromosomal evolution with amplification/deletion of constitutive heterochromatin and rearrangements including fusions, fissions, and pericentric inversions.

Pattern of nipple use by puppies: A comparison of the dingo (Canis dingo) and the domestic dog (Canis familiaris).

2016 ◽  
Vol 130 (3) ◽  
pp. 269-277 ◽  
Author(s):  
Robyn Hudson ◽  
Heiko G. Rödel ◽  
Marise Trejo Elizalde ◽  
Lourdes Arteaga ◽  
Gerard A. Kennedy ◽  
...  
Keyword(s):  
Canis Familiaris ◽  
Domestic Dog

CHROMOSOMES AND DNA OF MICROTUS II. CONFIRMATION OF DELETION OF CONSTITUTIVE HETEROCHROMATIN IN M. AGRESTIS CELLS IN VITRO

1977 ◽  
Vol 19 (3) ◽  
pp. 537-541 ◽  
Author(s):  
J. E. K. Cooper
Keyword(s):  
Somatic Cell ◽  
Cell Line ◽  
Cell Lines ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Constitutive Heterochromatin ◽  
The Other ◽  
Microtus Agrestis ◽  
C Banding

The distribution of constitutive heterochromatin has been examined by C-banding in two somatic cell lines, grown in vitro, from a female Microtus agrestis. One line retains one intact X chromosome together with the short arm of the other X chromosome, while the other cell line retains only the short arm of one X chromosome. Thus, each cell line has lost substantial amounts of heterochromatin from the sex chromosomes, but this material has been deleted from the cells, and not translocated to other chromosomes. Nonetheless, both cell lines continue to propagate well in vitro.

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