GENIC ANALYSIS FOR ACID PHOSPHATASE ISOZYMES IN ORYZA PERENNIS AND O. SATIVA

1975 ◽  
Vol 17 (4) ◽  
pp. 637-650 ◽  
Author(s):  
Chiang Pai ◽  
T. Endo ◽  
H. I. Oka
Keyword(s):  
Acid Phosphatase ◽  
Statistical Analysis ◽  
Recessive Allele ◽  
Band Group ◽  
Starch Gel ◽  
Oryza Perennis ◽  
Silent Allele

It was concluded from genetic experiments that various acid phosphatase zymograms observed among the rice strains belonging to Oryza perennis Moench (wild) and O. sativa L. (cultivated) were controlled by alleles at three loci, Acp1, Acp2, and Acp3. At Acp1 seven codominant alleles were found each producing a group of three major (A, M, and C) and three minor (a, m, and c) bands on starch gel. The alleles determined mobility of the band group. Statistical analysis of the data taken from densitometric tracings of zymograms indicated that hybrids between strains with different mobilities would produce three major and three minor hybrid bands each occurring between two corresponding parental bands. In addition, a null form lacking all these bands was found that had a recessive allele, Acp1Nu1. The Acp1 locus was independent of Px1 (specifying peroxidase OC, 2A, and 4A bands), but was linked withPx2 (specifying peroxidase 4C band). The Acp2 locus carried two codominant alleles specifying bands Fa and Sa, respectively, and a recessive silent allele, Acp2Nu1. This locus was closely linked with Acp1, yet it was distinguished from the latter as different allelic combinations were observed among parental strains. At Acp3 alleles specifying bands B and b were found which were peculiar to O. perennis subsp. barthii; other perennis and sativa strains appeared to have their recessive silent alleles.

The Red Cell Acid Phosphatase Polymorphism in Greek b-Thalassemia Patients

1975 ◽  
Vol 24 (3-4) ◽  
pp. 337-338
Author(s):  
A. Kalos ◽  
K. Melissinos ◽  
A. Archimandritis ◽  
G. Kourounis ◽  
B. Angelopoulos
Keyword(s):  
Acid Phosphatase ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Red Cell ◽  
Gene Frequencies ◽  
Starch Gel ◽  
Acid Phosphatase Polymorphism ◽  
Cell Acid

Red cell acid phosphatase polymorphism was studied by starch gel electrophoresis in 70 b-thalassemia patients and in 310 healthy Greeks. Our results gave the following gene frequencies: b-thalassemia patients: pa 0.321, pb 0.643, pc 0.036; healthy Greeks: pa 0.302, pb 0.653, pc 0.045. No statistically significant differences were found between the two groups.

scholarly journals The distribution of some hydrolases in glomeruli and tubular fragments prepared from rat kidney by zonal centrifugation

1971 ◽  
Vol 122 (5) ◽  
pp. 641-645 ◽  
Author(s):  
D. G. Taylor ◽  
R. G. Price ◽  
D. Robinson
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Gel Electrophoresis ◽  
Leucine Aminopeptidase ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Starch Gel Electrophoresis ◽  
Multiple Forms ◽  
Rat Kidney Cortex ◽  
Starch Gel

1. A collagenase digest of rat kidney cortex was separated into four bands by zonal centrifugation. 2. Two of these bands were shown by light-microscopy to contain glomeruli and tubular fragments, which were free from each other and well separated from other renal material. 3. Protein, N-acetyl-β-glucosaminidase, 5′-nucleotidase, l-leucine β-naphthylamidase, leucine aminopeptidase, acid phosphatase and alkaline phosphatase were assayed across the gradient. 4. The greater proportion of these enzyme activities was recovered in the tubular fragments and acid phosphatase was the only enzyme detected in significant amounts in the glomeruli. 5. Tubular fragments and glomeruli were sedimented and multiple forms of β-naphthylamidase, N-acetyl-β-glucosaminidase, acid phosphatase and alkaline phosphatase were investigated by starch-gel electrophoresis.

scholarly journals The genetics of Dacus oleae: II. The genetics of two adult esterases

1968 ◽  
Vol 12 (1) ◽  
pp. 1-9 ◽  
Author(s):  
E. Zouros ◽  
S. Tsakas ◽  
C. B. Krimbas
Keyword(s):  
Gel Electrophoresis ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Starch Gel Electrophoresis ◽  
Dacus Oleae ◽  
Lipase Gene ◽  
Multiple Alleles ◽  
Olive Fruit ◽  
Starch Gel ◽  
Silent Allele

The genetics of two esterase systems in adults of the olive fruit fly Dacus oleae (Gmel.) have been studied by starch gel electrophoresis. Two unlinked autosomal genes, each with multiple alleles, control the synthesis of a number of isozymes. Gene A controls the synthesis of an acetylcholinesterase, and gene B that of a pseudocholinesterase or lipase. Gene B produces hybrid enzyme in heterozygotes for active alleles. In crosses the segregation of seven active and one silent allele in gene A, and of six active and one silent allele in gene B, have been studied. Six more active alleles for gene A and five active alleles of gene B have not been studied in this paper.

GENETIC STUDIES OF ISOZYMES IN NEUROSPORA. I. A STUDY OF EIGHT SPECIES

1971 ◽  
Vol 13 (2) ◽  
pp. 298-305 ◽  
Author(s):  
M. Mohan Reddy ◽  
S. F. H. Threlkeld
Keyword(s):  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Phosphatase Activity ◽  
Gel Electrophoresis ◽  
Acid Phosphatase Activity ◽  
Starch Gel Electrophoresis ◽  
Acid Phosphatases ◽  
Genetic Studies ◽  
Starch Gel

Mycelial extracts of 34 strains representing eight species of the genus Neurospora were subjected to acrylamide and starch gel electrophoresis to detect sites of esterase, lactate dehydrogenase, amylase, peroxidase, and acid phosphatase activity. Nine isozymes of esterases, four isozymes of lactate dehyrogenases, three isozymes of peroxidases, and two isozymes of acid phosphatases were detected on the gels for the species. The application of zymograms as a biochemical means to characterize species is discussed.

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