CHROMOSOME NUMBER, VOLUME AND NUCLEAR VOLUME RELATIONSHIPS IN A POLYPLOID SERIES (2X-20X) OF THE GENUS RUMEX

1971 ◽  
Vol 13 (4) ◽  
pp. 842-863 ◽  
Author(s):  
S. Ichikawa ◽  
A. H. Sparrow ◽  
C. Frankton ◽  
Anne F. Nauman ◽  
E. B. Smith ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Abrupt Change ◽  
Diploid Species ◽  
Nuclear Volume ◽  
Polyploid Series ◽  
Unidentified Species

Ninety-one acquisitions of the genus Rumex obtained from various sources were examined taxonomically and cytologically. These acquisitions included 36 species plus 2 unidentified species. The chromosome numbers counted were 2n = 14 (or 15), 16, 18, 20, 40, 42, 60, 80, 100, 120, 140, 160, ca. 170, 180 and ca. 200. The count of 2n = 180 made on one of the R. orbiculatus acquisitions is a new count for this genus, but other acquisitions had 160 and ca. 170. First counts were obtained for four species, R. frutescens (2n = 160), R. crystallinus (2n = 60), R. cristatus (2n = 80) and R. tenax (2n = 80). The count of 2n = 160 for R. frutescens is the highest chromosome number ever reported in the section Axillares. The chromosome numbers determined in R. palustris (2n = 60), R. confertus (2n = 100), R. arcticus (2n = 120) and R. aquaticus (2n = 140) differ from previously published counts. Our counts for eight other species support one of the previous counts where two or more counts are reported. It is shown that the species of the subgenus Acetosa sections Acetosa and Vesicarii and of the subgenus Platypodium have relatively large chromosomes, those of the subgenus Acetosa section Scutati and of the subgenus Acetosella have medium-sized chromosomes, and the members of the subgenus Rumex sections Axillares and Rumex have smaller chromosomes. The chromosomes of the diploid species of the section Rumex were larger than those of the polyploids (4x to 20x) of the same section. Within the section Rumex the log of nuclear volume increased with increasing ploidy, with an abrupt change (decrease) in slope between the 12x and 14x levels.

scholarly journals Interphase nuclear phenotype and chromosomal variation in Cynodon dactylon (L.) pers

2019 ◽  
Vol 27 ◽  
pp. 133-141
Author(s):  
SK Nitu ◽  
SMS Islam ◽  
H Tarique
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Somatic Chromosome ◽  
Cynodon Dactylon ◽  
Nuclear Organization ◽  
Nuclear Volume ◽  
Chromosomal Variation ◽  
Nuclear Area ◽  
Somatic Chromosome Number ◽  
Interphase Chromosome

Interphase nuclear phenotype in different accessions (Acc.) of Cynodon dactylon studied in the present experiment showed chromocentric nuclear organization and the chromocenters were found to be visible clearly. The chromocenter numbers were not same and sometimes it was found to be significantly less and never more than total number of chromosomes. Percentages of heterochromatin values were expressed per nuclear area and the values range from 19.759% (Acc. 16) to 66.022% (Acc.18). Nuclear volume as well as interphase chromosome volume was found to vary 0.674 μm3 (Acc.6) to 41.921 μm3 (Acc.10) and from 0.028 μm3 (Acc. 6) to 1.905 μm3 (Acc. 10), respectively. The somatic chromosome number found to vary from 12 to 40. 2n = 18 chromosomes were found in eight accessions of C. dactylon. Only one accession was found to be tetraploid and rest of them aneuploid whose chromosome numbers were 12, 14, 16, 22, 24, 26, 32, 40 etc. The availability of aneuploid shows great aspects of forage breeding programme. J. bio-sci. 27: 133-141, 2019

scholarly journals Chromosome numbers of some species of Pteris (Pteridaceae) in Java, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2118-2126
Author(s):  
TITIEN NGATINEM PRAPTOSUWIRYO ◽  
MUGI MUMPUNI
Keyword(s):  
Chromosome Number ◽  
Morphological Variation ◽  
Chromosome Numbers ◽  
Diploid Species ◽  
Somatic Chromosome Number ◽  
Chromosome Counts ◽  
Ploidy Levels ◽  
Central Java ◽  
Fern Species ◽  
Pteris Multifida

Praptosuwiryo TNg, Mumpuni M. 2018. Chromosome numbers of some species of Pteris (Pteridaceae) in Java, Indonesia. Biodiversitas 19: 2118-2126. Pteris L. (Pteridaceae) is a large fern genus consisting of about 250 species which distributed predominantly in tropical and subtropical countries. The genus grows in a diversity of ecosystems, either terrestrially or lithophytically, although most species occur in the forest. A study of the cytology of Pteris in Java is being undertaken for the conceptual understanding of the interrelationships between various fern species in the Malesian region. This aims of our study are: (1) to observe somatic chromosome number of some species of Pteris in Java; (2) to determine the reproduction types of the observed species by counting the spore number per sporangium; (3) to discuss polyploidy in the species in relation to plant morphological variation across their geographical distribution. Chromosome counts for eight species are reported. Pteris biaurita is an apogamous diploid species (2n = 58). Pteris ensiformis var. ensiformis has chromosome numbers of 2n = 87 (apogamous triploid) and 2n = 116 (sexual tetraploid), while                 P. ensiformis var. victoriae has a chromosome number 2n = 58 and is a sexual diploid. Pteris fauriei is an apogamous triploid species (2n = 87). Pteris longipinnula has a chromosome number of 2n = 116 (tetraploid). Pteris multifida and P. vittata are sexual tetraploids (2n=116). Pteris tripartita has two ploidy levels (sexual diploid and tetraploid). Another currently unplaced Javanese Pteris sp. has 2n = 87 (apogamous triploid). Diploid P. biaurita, tetraploid P. ensiformis, triploid P. fauriei, diploid P. tripartita, and teraploid P. vittata are new record cytotypes for Central Java. Polyploidy in the genus in relation to plant morphological variation across its geographical range is discussed.

scholarly journals Estimating Nuclear DNA Content in Peach and Related Diploid Species Using Laser Flow Cytometry and DNA Hybridization

1994 ◽  
Vol 119 (6) ◽  
pp. 1312-1316 ◽  
Author(s):  
W. Vance Baird ◽  
Agnes S. Estager ◽  
John K. Wells
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Genome ◽  
Nuclear Dna Content ◽  
Gene Copy ◽  
Polyploid Series

Using laser flow cytometry, nuclear DNA amounts were estimated for 12 Prunus species, representing three subgenera [Prunophora (Prunus), Amygdalus, and Cerasus (Lithocerasus)], two interspecific hybrids, four cultivars, and a synthetic polyploid series of peach consisting of haploids, diploids, triploids, and tetraploids (periclinal cytochimeras). Peach nuclear DNA content ranged from 0.30 pg for the haploid nuclei to 1.23 pg for the tetraploid nuclei. The diploid genome of peach is relatively small and was estimated to be 0.60±0.03 pg (or 5.8×108 nucleotide base pairs). The polyploid series represented the expected arithmetic progression, as genome size positively correlated with ploidy level (i.e., DNA content was proportional to chromosome number). The DNA content for the 12 diploid species and two interspecific diploid hybrids ranged from 0.57 to 0.79 pg. Genome size estimates were verified independently by Southern blot analysis, using restriction fragment length polymorphism clones as gene-copy equivalents. Thus, a relatively small and stable nuclear genome typifies the Prunus species investigated, consistent with their low, basic chromosome number (× = 8).

Cytotaxonomy of Allium (Amaryllidaceae) subgenera Cyathophora and Amerallium sect. Bromatorrhiza

Phytotaxa ◽  
2017 ◽  
Vol 331 (2) ◽  
pp. 185 ◽  
Author(s):  
MIN-JIE LI ◽  
XIAN-LIN GUO ◽  
JUAN LI ◽  
SONG-DONG ZHOU ◽  
QING LIU ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Karyotype Evolution ◽  
Diploid Species ◽  
Basic Chromosome Number ◽  
Molecular Evidence ◽  
Basic Chromosome ◽  
Karyotype Asymmetry

In the present study, we examined the karyotype data of subg. Cyathophora and sect. Bromatorrhiza, to determine some disputed karyotypes (e.g., A. spicatum and A. fasciculatum), and further to estimate the karyotype evolution along their phylogenetic frameworks. Our results revealed a fairly stable basic chromosome number (x = 8) in subg. Cyathophora, and we therefore revised x = 8 as the basic chromosome number of A. spicatum, rather than x = 10 mostly due to misidentifications concerning A. fasciculatum. The karyotype asymmetry analyses for subg. Cyathophora indicated that, the karyotype evolution for diploid species showing a high karyotype similarity was mainly due to intrachromosomal changes, while the interchromosomal changes were linked to the evolution of tetraploid populations. However, indeed different dysploid basic chromosome numbers (x = 7, 10, 11) and greatly different karyotype patterns occurred in sect. Bromatorrhiza, corresponding to the subsections revealed by molecular evidence. The combined evidence suggested that species with x = 11 compose a segmental allotriploid complex. It was also indicated that karyotype pattern of polyploids usually is closely related with  their diploid progenitors.

scholarly journals Variation in Ribosomal DNA in the Genus Trifolium (Fabaceae)

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1771
Author(s):  
Radka Vozárová ◽  
Eliška Macková ◽  
David Vlk ◽  
Jana Řepková
Keyword(s):  
Chromosome Number ◽  
Ribosomal Dna ◽  
Chromosome Numbers ◽  
Evolutionary History ◽  
Diploid Species ◽  
Basic Chromosome Number ◽  
Rdna Site ◽  
Ancestral State ◽  
Basic Chromosome ◽  
26S Rdna

The genus Trifolium L. is characterized by basic chromosome numbers 8, 7, 6, and 5. We conducted a genus-wide study of ribosomal DNA (rDNA) structure variability in diploids and polyploids to gain insight into evolutionary history. We used fluorescent in situ hybridization to newly investigate rDNA variation by number and position in 30 Trifolium species. Evolutionary history among species was examined using 85 available sequences of internal transcribed spacer 1 (ITS1) of 35S rDNA. In diploid species with ancestral basic chromosome number (x = 8), one pair of 5S and 26S rDNA in separate or adjacent positions on a pair of chromosomes was prevalent. Genomes of species with reduced basic chromosome numbers were characterized by increased number of signals determined on one pair of chromosomes or all chromosomes. Increased number of signals was observed also in diploids Trifolium alpestre and Trifolium microcephalum and in polyploids. Sequence alignment revealed ITS1 sequences with mostly single nucleotide polymorphisms, and ITS1 diversity was greater in diploids with reduced basic chromosome numbers compared to diploids with ancestral basic chromosome number (x = 8) and polyploids. Our results suggest the presence of one 5S rDNA site and one 26S rDNA site as an ancestral state.

scholarly journals Chromosome numbers in Begonia.

1969 ◽  
Vol 17 (3) ◽  
pp. 189-202 ◽  
Author(s):  
R.A.H. Legro ◽  
J. Doorenbos
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Somatic Chromosome ◽  
Polyploid Series ◽  
First Time ◽  
Somatic Chromosome Numbers

The somatic chromosome numbers of 100 Begonia species were counted, of which 72 have been determined for the first time. Eighteen different numbers have been established, ranging between 22 and 156, fifteen of which could be fitted into a tentative scheme of five polyploid series. The most common numbers were 22 (14 species), 28 (35 species) and 56 (17 species). The species have been grouped into sections according to Irmscher. In some cases these sections appeared to have a characteristic chromosome number whereas in others, e.g. Pritzelia and Begoniastrum, a great diversity in chromosome number was encountered. [For earlier work see H.A., 39: 5182.]-Univ. Wageningen. (Abstract retrieved from CAB Abstracts by CABI’s permission)

A contribution to the taxonomy of the Oxytropis campestris complex in northwestern North America

1980 ◽  
Vol 58 (16) ◽  
pp. 1820-1831 ◽  
Author(s):  
Wayne J. Elisens ◽  
John G. Packer
Keyword(s):  
North America ◽  
Chromosome Number ◽  
Cell Size ◽  
Guard Cell ◽  
Chromosome Numbers ◽  
Species Status ◽  
Polyploid Series ◽  
Continental Divide ◽  
Northwestern North America

The Oxytropis campestris complex in northwestern North America is a polyploid series comprising at least seven morphologically and geographically distinct taxa. In light of the data of the present study, the authors propose that five taxa be reelevated to species status: O. cusickii Greenm., O. monticola Gray, O. columbiana St. John. O. jordalii Porsild, O. varions (Rydb.) K. Schum.; and that two taxa be recombined as subspecies: O. monticola Gray ssp. dispar (A. Nels.) Elisens & Packer and O. jordalii Porsild ssp. davisii (Welsh) Elisens & Packer.Three different chromosome numbers are present in the complex and represent the tetraploid (2n = 32), hexaploid (2n = 48), and dodecaploid (2n = 96) condition. Three species have uniform chromosome numbers (O. cusickii, 2n = 48; O. jordalii, 2n = 32; and O. columbiana, 2n = 48), two taxa, O. varians and O. monticola ssp. monticola, exhibit two different chromosome numbers. No attempt to subdivide O. varians was undertaken as; with the exception of guard cell size, no differences were observed between hexaploid and dodecaploid representatives. At least two distinct entities appear to be present in O. monticola ssp. monticola, for, while morphologically, cytologically (2n = 32), and ecologically uniform east of the continental divide, it is quite variable in appearance and has a different chromosome number (2n = 48) west of the divide.

scholarly journals New Contribution to the Knowledge on the Chromosome Numbers of Turkish Cerambycidae (Coleoptera)

2021 ◽  
Vol 69 (2) ◽  
pp. 93-99
Author(s):  
Yavuz Koçak ◽  
Elmas Yağmur
Keyword(s):  
Chromosome Number ◽  
Sex Determination ◽  
Chromosome Numbers ◽  
Chromosome Count ◽  
Chromosomal Number ◽  
The Poor ◽  
Karyological Data ◽  
First Time

Information on the karyotypes of Turkish species of Cerambycidae is scanty. Our study contributes to the knowledge of the karyological data (chromosomal number and mechanism of sex determination) of five Turkish longicorn beetles; karyotypes of four taxa, one endemic, are described for the first time and for the remaining one, Purpuricenus budensis (Götz, 1783), the previously published chromosome count is confirmed. The chromosome number of Purpuricenus desfontainii inhumeralis Pic, 1891 and Purpuricenus budensis (Götz, 1783) (Cerambycinae, Trachyderini) was found to be 2n = 28 (13 + Xyp); Clytus rhamni Germar, 1817 and Plagionotus floralis (Pallas, 1773) (Cerambycinae, Clytini) 2n = 20 (9 + Xyp); and the endemic Dorcadion triste phrygicum Peks, 1993 (Lamiinae, Dorcadionini) 2n = 24 (11 + Xyp). In view of the paucity of data available until now, our study is important for both to improve the poor karyological knowledge of Turkish Cerambycidae and to provide an incentive for other researchers.

scholarly journals Karyotype Analysis of Alnus Mill. (Betulaceae) Species Originating From Northeastern Asia

2010 ◽  
Vol 59 (1-6) ◽  
pp. 219-223 ◽  
Author(s):  
Liu Jun ◽  
Ren Bao-Qing ◽  
Luo Peigao ◽  
Ren Zhenglong
Keyword(s):  
Chromosome Number ◽  
Chromosome Numbers ◽  
Karyotype Analysis ◽  
Full Potential ◽  
Northeastern Asia ◽  
Ploidy Levels ◽  
Genetic Makeup ◽  
Fossil Records

Abstract Alnus Mill. (alder) is an ecologically valuable tree genus. It is essential to study its genetic makeup in order to use alder trees to their full potential. Five specimens from four Alnus species (A. mandshurica, A. pendula, A. sibirica, and A. sieboldiana), found in northeastern Asia, were subjected to karyotype analysis. The analysis showed that these tree samples could be divided into three categories based on chromosome numbers or ploidy levels: viz., 2n = (4x) = 28, 2n = (8x) = 56 and 2n = (16x) = 112. The differences in chromosome number and karyotype parameters among Alnus species and even within the same species possibly resulted from natural polyploidization. Comparing the chromosome numbers of Alnus species in China with those in Japan showed that there appear to be only two categories in China, whereas there are up to five categories in Japan. The earliest fossil records of Alnus pollen were also discovered in Japan. We conclude that the center origin of Alnus spp. is Japan rather than China.

scholarly journals Relations and evolution in Cheilanthes (Sinopteridaceae, Pteridophita) in Macaronesia and Mediterranean area, deduced from genome analysis of their hybrids

1983 ◽  
Vol 8 ◽  
pp. 101-126 ◽  
Author(s):  
G. Vida ◽  
A. Major ◽  
T. Reichstein
Keyword(s):  
Chromosome Number ◽  
Genome Analysis ◽  
Chromosome Doubling ◽  
Diploid Species ◽  
Mediterranean Area ◽  
Basic Chromosome Number ◽  
The Other ◽  
Experimental Conditions ◽  
Natural Group ◽  
Basic Chromosome

Nine species of "Cheilantoid ferns" are known to grow in Macaronesia and the Mediterranean basin. Two of them (lacking a pseudo-indusium and having the basic chromosome number X = 29), both aggregate species which we prefer to retain in Notholaena, are not included in this study. The other seven species (with distinct pseudo-indusium and the basic chromosome number X = 30), which we accept as members of the genus Cheilanthes Sw. sensu stricto, were subjected to detailed genome analysis of their natural and experimentally produced hybrids and shown to represent an aggregate of four very distinct ancestral diploids and three allotetraploids. The latter must have once been formed by chromosome doubling in the three diploid hybrids of C. maderensis Lowe with the other three diploid species. Theoretically three more allotetraploids would be possible but their formation has obviously been prevented by the geographical separation of the three respective diploids. The most widely distributed of the tetraploids, i.e. C. pteridioides (Reich.) C.Chr. has also been resynthesized from its ancestors (still sympatric) under experimental conditions. The intermediate morphology of the allotetraploids (as compared with their diploid ancestors) is obviously the reason why their status and existence has so long escaped recognition in Europe. These seven species form a natural group and, in our opinion, should not be divided into sections.

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