COCCIDIOIDES IMMITIS: CYTOLOGICAL STUDY ON THE FORMATION OF THE ARTHROSPORES

1969 ◽  
Vol 11 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Kyung Joo Kwon-Ciiung

Somatic nuclear division and the manner of arthrospore formation in saprophytic phase of Coccidioides immitis have been studied. Two distinct nuclear cycles, one occurring during the active growth in young hyphae and another occurring in old thin hyphae or during arthrospore formation, were observed. The first cycle consisted of several stages starting from round resting nuclei → ring stage → V stage → and filaments which divide longitudinally. The two daughter nuclear filaments reorganize into round nuclei. The second cycle is more simple than the first. The round resting nuclei become elongated and constricted at the middle, dividing vertically. The chromosome number appears to be 3. The metaphase plares or spindle apparatus were not seen.The fertile hyphae develop septa not basipetally but synchronously. Alternate cells, after being thus delimited, increase in size and in thickness of wall becoming spores while the intervening cells gradually lose cytoplasm. The nuclei remain, without cytoplasm, in the intervening cells until the adjacent spores are completely matured. The spores are released by fragmentation of the walls of the sterile segments. The relationship of C. immitis to the members of Gymnoascaceae has been discussed.

Parasitology ◽  
1954 ◽  
Vol 44 (1-2) ◽  
pp. 120-131 ◽  
Author(s):  
Ann Bishop

In strains ofPlasmodium gallinaceumpassaged by blood-inoculation, through young birds treated with small, but effective doses of proguanil, sulphadiazine or 2:4-diamino-6:7-diisopropylpteridine, the numbers of gametocytes produced were much greater than in the parent strain passaged by the same method.The effect of the drugs upon gametocyte production appears to be a long term one, since no change in gametocyte numbers was observed in birds treated with proguanil or sulphadiazine for only a few days.The relationship of these drugs to nuclear division and the synthesis of nucleic acids is discussed in the light of the observed increase in gametocyte-production in drug-treated strains ofP. gallinaceum.


1973 ◽  
Vol 69 (3) ◽  
pp. 71-89 ◽  
Author(s):  
Ann R. Sanderson

SynopsisKaryograms prepared from ovarian and blastoderm cells of the parthenogenetic Australian Brown Vegetable Weevil demonstrate a consistent triploid condition with 30 chromosomes which can be grouped into 10 sets of homologues. Meiosis is replaced by a single mitotic-like division in which 30 univalent chromosomes, each composed of two chromatids, divide equationally between an ootid nucleus and a single polar nucleus. Prior to the differentiation of the oocytes a peculiar bouquet stage occurs in the cells of the end chamber of each ovariole, but the significance of this phase is not known. Arrested development in eggs from individuals of low fertility is investigated and the relationship of body size and chromosome number is discussed.


2018 ◽  
Author(s):  
Emily L. Kurdzo ◽  
Hoa H Chuong ◽  
Dean S. Dawson

ABSTRACTIn meiosis I, homologous chromosomes segregate away from each other - the first of two rounds of chromosome segregation that allow the formation of haploid gametes. In prophase I, homologous partners become joined along their length by the synaptonemal complex (SC) and crossovers form between the homologs to generate links called chiasmata. The chiasmata allow the homologs to act as a single unit, called a bivalent, as the chromosomes attach to the microtubules that will ultimately pull them away from each other at anaphase I. Recent studies, in several organisms, have shown that when the SC disassembles at the end of prophase, residual SC proteins remain at the homologous centromeres providing an additional link between the homologs. In budding yeast, this centromere pairing is correlated with improved segregation of the paired partners in anaphase. However, the causal relationship of prophase centromere pairing and subsequent disjunction in anaphase has been difficult to demonstrate as has been the relationship between SC assembly and the assembly of the centromere pairing apparatus. Here, a series of in-frame deletion mutants of the SC component Zip1 were used to address these questions. The identification of separation-of-function alleles that disrupt centromere pairing, but not SC assembly, have made it possible to demonstrate that centromere pairing and SC assembly have mechanistically distinct features and that prophase centromere pairing function of Zip1 drives disjunction of the paired partners in anaphase I.AUTHOR SUMMARYThe generation of gametes requires the completion of a specialized cell división called meiosis. This division is unique in that it produces cells (gametes) with half the normal number of chromosomes (such that when two gametes fuse the normal chromosome number is restored). Chromosome number is reduced in meiosis by following a single round of chromosome duplication with two rounds of segregation. In the first round, meiosis I, homologous chromosomes first pair with each other, then attach to cellular cables, called microtubules, that pull them to opposite sides of the cell. It has long been known that the homologous partners become linked to each other by genetic recombination in a way that helps them behave as a single unit when they attach to the microtubules that will ultimately pull them apart. Recently, it was shown, in budding yeast and other organisms, that homologous partners can also pair at their centromeres. Here we show that this centromere pairing also contributes to proper segregation of the partners away from each other at meiosis I, and demonstrate that one protein involved in this process is able to participate in multiple mechanisms that help homologous chromosomes to pair with each other before being segregated in meiosis I.


1978 ◽  
Vol 20 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Byron L. Burson

Paspalum conspersum Schrad. ex Schult., 2n = 4x = 40, was crossed with P. intermedium Munro ex Morong, 2n = 2x = 20, and P. jurgensii Hackel, 2n = 2x = 20, and the hybrids were studied cytologically to determine the relationship between these species. Thirteen P. intermedium × P. conspersum hybrid plants were produced; however, only eight survived. They had a chromosome number of 2n = 3x = 30. Meiosis was irregular with a chromosome pairing relationship of 19.87 univalents, 5.03 bivalents, and 0.03 trivalents per cell. These findings suggested that the two species have a partially homologous genome. The two hybrids obtained from the cross between P. jurgensii and P. conspersum had a chromosome number of 2n = 3x = 30. The mean chromosome pairing in these hybrids was 10.12 univalents. 9.86 bivalents, 0.08 trivalents, and 0.004 quadrivalents. The close bivalent pairing and a mean frequency of 9.86 bivalents suggested that the P. jurgensii genome was homologous to one genome of P. conspersum. Limited autosyndetic pairing of the P. conspersum chromosomes was also detected in both groups of hybrids. A standardization of genome formulas for the genus was proposed in which P. intermedium, P. jurgensii, and P. conspersum were represented by genome formulas of II, JJ, and I2I2 JJ, respectively. The genome relationships and formulas were discussed for other related Paspalum species.


1990 ◽  
Vol 38 (1) ◽  
pp. 33 ◽  
Author(s):  
JD Murray ◽  
SC Donnellan ◽  
GM Mckay ◽  
RH Rofe ◽  
PR Baverstock ◽  
...  

The standard and C-banded (four species) karyotypes of six species of the family Petauridae (Gymnobelideus leadbeateri, Petaurus australis, P. breviceps, P. norfolcensis, Dactylopsila trivirgata and Dactylonax palpator) are described. The G-banded karyotype of P. norfolcensis is also described. Gymnobelideus and Petaurus have diploid chromosome number of 22. All three species of Petaurus have a similar karyotype, consisting of biarmed autosomes and very small sex chromosomes, which differs from Gymnobelideus by a minimum of one chromosomal rearrangement of each autosome. Dactylopsila and Dactylonax have similar karyotypes with diploid chromosome numbers of 18. The relationship of these two genera to Petaurus is still uncertain but all members of this family differ from Pseudocheiridae in the small size of the sex chromosomes.


Paleobiology ◽  
1980 ◽  
Vol 6 (02) ◽  
pp. 146-160 ◽  
Author(s):  
William A. Oliver

The Mesozoic-Cenozoic coral Order Scleractinia has been suggested to have originated or evolved (1) by direct descent from the Paleozoic Order Rugosa or (2) by the development of a skeleton in members of one of the anemone groups that probably have existed throughout Phanerozoic time. In spite of much work on the subject, advocates of the direct descent hypothesis have failed to find convincing evidence of this relationship. Critical points are:(1) Rugosan septal insertion is serial; Scleractinian insertion is cyclic; no intermediate stages have been demonstrated. Apparent intermediates are Scleractinia having bilateral cyclic insertion or teratological Rugosa.(2) There is convincing evidence that the skeletons of many Rugosa were calcitic and none are known to be or to have been aragonitic. In contrast, the skeletons of all living Scleractinia are aragonitic and there is evidence that fossil Scleractinia were aragonitic also. The mineralogic difference is almost certainly due to intrinsic biologic factors.(3) No early Triassic corals of either group are known. This fact is not compelling (by itself) but is important in connection with points 1 and 2, because, given direct descent, both changes took place during this only stage in the history of the two groups in which there are no known corals.


Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


Author(s):  
Leon Dmochowski

Electron microscopy has proved to be an invaluable discipline in studies on the relationship of viruses to the origin of leukemia, sarcoma, and other types of tumors in animals and man. The successful cell-free transmission of leukemia and sarcoma in mice, rats, hamsters, and cats, interpreted as due to a virus or viruses, was proved to be due to a virus on the basis of electron microscope studies. These studies demonstrated that all the types of neoplasia in animals of the species examined are produced by a virus of certain characteristic morphological properties similar, if not identical, in the mode of development in all types of neoplasia in animals, as shown in Fig. 1.


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