Genomic characteristics of a diploid F4 festulolium hybrid (Lolium multiflorum × Festuca arundinacea)

Genome ◽  
2012 ◽  
Vol 55 (8) ◽  
pp. 599-603 ◽  
Author(s):  
Yukio Akiyama ◽  
Kenji Kimura ◽  
Hitomi Yamada-Akiyama ◽  
Akito Kubota ◽  
Yoshinori Takahara ◽  
...  
Keyword(s):  
Festuca Arundinacea ◽  
Agronomic Traits ◽  
Genetic Relationships ◽  
Lolium Multiflorum ◽  
Summer Temperature ◽  
Chromosome Engineering ◽  
Chromosome Architecture ◽  
Potential Applications ◽  
High Ploidy ◽  
Heat Stress Tolerance

The grass festulolium, a hybrid between the genera Festuca and Lolium , has a variety of beneficial agronomic attributes derived from both parents. Compared with high-ploidy festulolium, diploid festulolium is well suited to stabilizing ploidy and for studying agronomic traits and genetic relationships. We sought to produce a diploid festulolium hybrid that was resistant to summer depression, by hybridizing diploid Lolium multiflorum Lam. and hexaploid Festuca arundinacea Schreb., which has a high tolerance to summer depression. We obtained seven diploid F4 plants that were capable of surviving the extremely hot summer in Morioka, Japan, in 2010, which was 2.7 °C higher than the average summer temperature. The observed resistance to summer depression in these plants was likely due to heat stress tolerance. The genomic constitutions of these seven hybrids were analyzed by GISH, and the chromosomal characteristics of a single diploid F4 was analyzed by FISH using rDNA probes. The results showed that although no Festuca-specific genome remained in any of the seven diploid F4 plants, extensive chromosomal rearrangement was observed in one of them. Our findings suggested that hybridizing diploid L. multiflorum and hexaploid F. arundinacea may be useful for modifying chromosome architecture in the Lolium genome with potential applications in chromosome engineering.

scholarly journals Evaluating the genetic variability of rubber hybrid progenies of the two crosses PB260 x RO44/71 and PB260 x RO62/54 by RAPD technique

2019 ◽  
Vol 2 (3) ◽  
pp. 36-43
Author(s):  
Thien Minh Nguyen ◽  
Tien Thi My Pham
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Field Experiments ◽  
Agronomic Traits ◽  
Genetic Relationships ◽  
Genetic Material ◽  
Population Based ◽  
Shannon Index ◽  
Polymorphic Bands

The agronomic values of this population have been evaluated in the field experiments based on their phenotypic performance of agronomic traits, but the genetic variability of this population needs to be evaluated via techniques based on genetic material - DNA. In this study, the genetic variability in the investigated population of 71 hybrids and their parents was evaluated by RAPD technique, using eight selected arbitrarily primers; Genetic parameters and dendrogram expressing the genetic relationships among the investigated population were analyzed by GenALEx 6.1, Popgene 1.31 and NTSYSpc 2.1 softwares. Eight primers were used to generate the amplify products on each individual in the investigated population. From 74 genotypes, a total of 109 fragments were generated, among which, there were 89 polymorphic bands representing 81.65% with an average of 11 polymorphic bands/primer. Genetic similarity coefficient among the investigated population, based on DICE coefficient, ranged from 0.560 (LH05/0822 and PB260) to 0.991 (LH05/0781 and LH05/0841) with an average of 0,796, meaning that the genetic distance among ranged from 0.009 to 0.440 with an average of 0.231. The Shannon index and mean heterozygosity values were 0.328 and 0,176, respectively. This indicated that the progenies of the two investigated crosses possessed a relatively high range of genetic variability. The analysis of molecular variance (AMOVA) showed that genetic variation within population represented 62%, while genetic variation among two different crosses contributes 38% to the total genetic variability. Dendrogram based on DICE’s genetic similarity using UPGMA method showed that the hybrids divide into two major genetic groups (0.75), but the crosses were scattered independently of the hybrid.

scholarly journals Introgression Mapping of Genes for Winter Hardiness and Frost Tolerance Transferred from Festuca arundinacea into Lolium multiflorum

2007 ◽  
Vol 98 (4) ◽  
pp. 311-316 ◽  
Author(s):  
A Kosmala ◽  
Z Zwierzykowski ◽  
E Zwierzykowska ◽  
M Luczak ◽  
M Rapacz ◽  
...  
Keyword(s):  
Festuca Arundinacea ◽  
Lolium Multiflorum ◽  
Frost Tolerance ◽  
Winter Hardiness

Genetic diversity among Italian melon inodorus (Cucumis melo L.) germplasm revealed by ISSR analysis and agronomic traits

2011 ◽  
Vol 9 (2) ◽  
pp. 214-217 ◽  
Author(s):  
S. Sestili ◽  
A. Giardini ◽  
N. Ficcadenti
Keyword(s):  
Agronomic Traits ◽  
Genetic Relationships ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Phenotypic Traits ◽  
Phenotypic Data ◽  
Morphological And Molecular Data ◽  
And Cluster Analysis ◽  
Almost All ◽  
Issr Primers

The genetic relationships among 13 melon inodorus populations that were collected in southern Italy were assessed using 100 inter-simple-sequence repeat (ISSR) primers and 15 morphological traits. The dihaploid line Nad-1 and the cultivar Charentais-T, both of which belong to the botanical variety cantalupensis, were used as reference accessions in the molecular analysis. A total of 358 polymorphic bands were obtained from 39 of the 100 ISSR primers used, and 15 phenotypic traits were scored and used for genetic-similarity calculations and cluster analysis. The resulting dendrograms based on the ISSR and phenotypic data allowed almost all of the melon genotypes to be distinguished on the basis of the skin colour of the fruits. Mantel's test revealed a good correlation between the morphological and molecular data in their ability to detect genetic relationships among melon ecotypes (r = 0.50, P = 0.99). The data obtained confirm the effectiveness of this approach, and open new perspectives to reveal possible molecular associations with the phenotypic traits analysed.

scholarly journals Humic acid enhances heat stress tolerance via transcriptional activation of Heat-Shock Proteins in Arabidopsis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Joon-Yung Cha ◽  
Sang-Ho Kang ◽  
Imdad Ali ◽  
Sang Cheol Lee ◽  
Myung Geun Ji ◽  
...  
Keyword(s):  
Heat Stress ◽  
Humic Acid ◽  
Heat Shock ◽  
Stress Tolerance ◽  
Transcriptional Activation ◽  
Molecular Mechanisms ◽  
Agronomic Traits ◽  
Enrichment Analysis ◽  
Gene Families ◽  
Heat Stress Tolerance

Abstract Humic acid (HA) is composed of a complex supramolecular association and is produced by humification of organic matters in soil environments. HA not only improves soil fertility, but also stimulates plant growth. Although numerous bioactivities of HA have been reported, the molecular evidences have not yet been elucidated. Here, we performed transcriptomic analysis to identify the HA-prompted molecular mechanisms in Arabidopsis. Gene ontology enrichment analysis revealed that HA up-regulates diverse genes involved in the response to stress, especially to heat. Heat stress causes dramatic induction in unique gene families such as Heat-Shock Protein (HSP) coding genes including HSP101, HSP81.1, HSP26.5, HSP23.6, and HSP17.6A. HSPs mainly function as molecular chaperones to protect against thermal denaturation of substrates and facilitate refolding of denatured substrates. Interestingly, wild-type plants grown in HA were heat-tolerant compared to those grown in the absence of HA, whereas Arabidopsis HSP101 null mutant (hot1) was insensitive to HA. We also validated that HA accelerates the transcriptional expression of HSPs. Overall, these results suggest that HSP101 is a molecular target of HA promoting heat-stress tolerance in Arabidopsis. Our transcriptome information contributes to understanding the acquired genetic and agronomic traits by HA conferring tolerance to environmental stresses in plants.

Resistance of Lolium multiflorum/Festuca arundinacea hybrids to some diseases of their parent species

Euphytica ◽  
1974 ◽  
Vol 23 (2) ◽  
pp. 315-320 ◽  
Author(s):  
P. W. Wilkins ◽  
A. J. H. Carr ◽  
E. J. Lewis
Keyword(s):  
Festuca Arundinacea ◽  
Lolium Multiflorum ◽  
Parent Species
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