Haplotype combination of the bovine INSIG1 gene sequence variants and association with growth traits in Nanyang cattle

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 429-436 ◽  
Author(s):  
Jiajie Sun ◽  
Yuan Gao ◽  
Dong Liu ◽  
Wei Ma ◽  
Jing Xue ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Growth Traits ◽  
Regulatory Element ◽  
Sequence Variants ◽  
Coding Region ◽  
Dna Pooling ◽  
Proteolytic Activation ◽  
Haplotype Combination ◽  
Pcr Rflp ◽  
Sterol Regulatory Element

The insulin-induced gene 1 (INSIG1) gene encodes a protein that blocks proteolytic activation of sterol regulatory element binding proteins, which are transcription factors that activate genes that regulate cholesterol, fatty acid, and glucose metabolism. However, similar research for the bovine INSIG1 gene is lacking. Therefore, in this study, polymorphisms of the bovine INSIG1 gene were detected in 643 individuals from four cattle breeds by DNA pooling, forced PCR–RFLP, PCR–SSCP, and DNA sequencing methods. Only 10 novel SNPs were identified, which included four mutations in the coding region and the others in the introns. In Nanyang individuals, seven common haplotypes were identified based on four coding region SNPs. The haplotype GACT, with a frequency of 75.4%, was the most prevalent haplotypes and SNPs formed two linkage disequilibrium blocks with strong multi-allelic D′ (D′ = 1). Additionally, association analysis between mutations of the bovine INSIG1 gene and growth traits in Nanyang cattle at 6, 12, 18, and 24 months old was performed, and the results indicated that the polymorphisms were not significantly associated with body mass.

Genotypes and haplotype combination of ACSL3 gene sequence variants is associated with growth traits in Dezhou donkey

Gene ◽  
2020 ◽  
Vol 743 ◽  
pp. 144600 ◽  
Author(s):  
Zhenyu Lai ◽  
Shipeng Li ◽  
Fei Wu ◽  
Zihui Zhou ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Growth Traits ◽  
Sequence Variants ◽  
Haplotype Combination

Exploring polymorphisms and associations of the bovine MOGAT3 gene with growth traits

Genome ◽  
2012 ◽  
Vol 55 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Jiajie Sun ◽  
Chunlei Zhang ◽  
Xianyong Lan ◽  
Chuzhao Lei ◽  
Hong Chen
Keyword(s):  
Growth Traits ◽  
Enzymatic Reaction ◽  
Fatty Acyl ◽  
Missense Mutations ◽  
Coding Region ◽  
Dna Pooling ◽  
Qinchuan Cattle ◽  
Rate Limiting Step ◽  
Pcr Rflp ◽  
Acyl Coenzyme A

Monoacylglycerol acyltransferase (MGAT3, also known as MOGAT3) catalyzes the synthesis of diacylglycerol (DAG) using 2-monoacylglycerol and fatty acyl coenzyme A. This enzymatic reaction is believed to be an essential and rate-limiting step for the absorption of dietary fat in the small intestine. However, similar research for the bovine MOGAT3 gene is lacking. Therefore, in this paper, polymorphisms of the bovine MOGAT3 gene were detected in 1145 individuals from five cattle breeds by DNA pooling, PCR–RFLP, and DNA sequencing methods. The results showed that 26 novel SNPs were identified, which included 16 mutations in the coding region and the others in the introns. Additionally, association analysis between two missense mutations, g.A229G and g.G1627A, and growth traits in Nanyang cattle up to 2 years of age and adult Qinchuan cattle was performed. The results indicated that polymorphisms were significantly associated with Nanyang cattle, but no convincing associations were observed for Qinchuan cattle for the studied traits.

Haplotype combination of SREBP-1c gene sequence variants is associated with growth traits in cattle

Genome ◽  
2011 ◽  
Vol 54 (6) ◽  
pp. 507-516 ◽  
Author(s):  
Yong-Zhen Huang ◽  
Hua He ◽  
Jia-Jie Sun ◽  
Jing Wang ◽  
Zhuan-Jian Li ◽  
...  
Keyword(s):  
Growth Traits ◽  
Regulatory Element ◽  
Large Deletion ◽  
Silent Mutation ◽  
Sequence Variants ◽  
Missense Mutations ◽  
Cattle Breeds ◽  
Pcr Rflp ◽  
Element Binding Protein ◽  
Strong Candidate

The aim of this study was to examine the association of the SREBP-1c polymorphism with growth traits in cattle breeds. Five sequence variants (SVs) were identified within the bovine sterol regulatory element-binding protein-1c gene (SREBP-1c), using DNA sequencing, PCR, PCR–RFLP, and forced PCR–RFLP methods. These polymorphisms include three missense mutations (SV1, SV4, and SV5) in exons 7, 9, and 12, a silent mutation (SV3) in exon 9, and a large deletion (SV2) in intron 7. Overall, we report the validation of polymorphisms within the bovine SREBP-1c gene, and the haplotype variability and extent of linkage disequilibrium (LD) in 1061 individuals representing the five main cattle breeds from China. We also investigated haplotype frequencies and LD coefficients for five SVs in all study populations. LD and haplotype structure of SREBP-1c were different between breeds. The result of haplotype analysis of five SVs showed that 27 different haplotypes were identified by all breeds. Two haplotypes (Hap1 and Hap2) shared by all five populations accounted for 42.75%, 35.68%, 36.44%, 25.43%, and 96.26% of all haplotypes observed in the cattle breeds Nanyang, Qinchuan, Jiaxian, Jinnan, and Chinese Holstein, respectively. The statistical analyses indicated that one single SV and 38 combined haplotypes were significantly associated with growth traits in the Nanyang cattle population (P < 0.05 or P < 0.01). The results of this study suggest that the SREBP-1c gene possibly is a strong candidate gene that affects growth traits in the Chinese beef cattle breeding program.

Haplotype combination of the caprine PC1 gene sequence variants and association with growth traits in Chinese Haimen breed

2012 ◽  
Vol 93 (S1) ◽  
pp. 54-59 ◽  
Author(s):  
JIAJIE SUN ◽  
JING XUE ◽  
CHUNLEI ZHANG ◽  
XIANYONG LAN ◽  
CHUZHAO LEI ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Growth Traits ◽  
Sequence Variants ◽  
Haplotype Combination

Haplotype combination of the bovine CFL2 gene sequence variants and association with growth traits in Qinchuan cattle

Gene ◽  
2015 ◽  
Vol 563 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Yujia Sun ◽  
Xianyong Lan ◽  
Chuzhao Lei ◽  
Chunlei Zhang ◽  
Hong Chen
Keyword(s):  
Gene Sequence ◽  
Growth Traits ◽  
Sequence Variants ◽  
Qinchuan Cattle ◽  
Haplotype Combination

scholarly journals Sterol Regulatory Element Binding Proteins in Fungi: Hypoxic Transcription Factors Linked to Pathogenesis

2010 ◽  
Vol 9 (3) ◽  
pp. 352-359 ◽  
Author(s):  
Clara M. Bien ◽  
Peter J. Espenshade
Keyword(s):  
Transcription Factors ◽  
Binding Proteins ◽  
Regulatory Element ◽  
Antifungal Drugs ◽  
Adaptation To Hypoxia ◽  
Human Pathogens ◽  
Low Oxygen ◽  
Proteolytic Activation ◽  
Content Type ◽  
Sterol Regulatory Element

ABSTRACT Sterol regulatory element binding proteins (SREBPs) are membrane-bound transcription factors whose proteolytic activation is controlled by the cellular sterol concentration. Mammalian SREBPs are activated in cholesterol-depleted cells and serve to regulate cellular lipid homeostasis. Recent work demonstrates that SREBP is functionally conserved in fungi. While the ability to respond to sterols is conserved, fungal SREBPs are hypoxic transcription factors required for adaptation to a low-oxygen environment. In the fission yeast Schizosaccharomyces pombe, oxygen regulates the SREBP homolog Sre1 by independently controlling both its proteolytic activation and its degradation. SREBP is also required for adaptation to hypoxia in the human pathogens Cryptococcus neoformans and Aspergillus fumigatus. In these organisms, SREBP is required for virulence and resistance to antifungal drugs, making the SREBP pathway a potential target for antifungal therapy.

scholarly journals Identification of a degradation signal at the carboxy terminus of SREBP2: A new role for this domain in cholesterol homeostasis

2020 ◽  
Vol 117 (45) ◽  
pp. 28080-28091
Author(s):  
Daniel L. Kober ◽  
Shimeng Xu ◽  
Shili Li ◽  
Bilkish Bajaj ◽  
Guosheng Liang ◽  
...  
Keyword(s):  
Regulatory Element ◽  
Proteasomal Degradation ◽  
Cholesterol Homeostasis ◽  
Binding Motif ◽  
Animal Cells ◽  
Proteolytic Activation ◽  
Lipogenic Genes ◽  
Amino Terminal ◽  
Cholesterol Levels ◽  
Sterol Regulatory Element

Lipid homeostasis in animal cells is maintained by sterol regulatory element-binding proteins (SREBPs), membrane-bound transcription factors whose proteolytic activation requires the cholesterol-sensing membrane protein Scap. In endoplasmic reticulum (ER) membranes, the carboxyl-terminal domain (CTD) of SREBPs binds to the CTD of Scap. When cholesterol levels are low, Scap escorts SREBPs from the ER to the Golgi, where the actions of two proteases release the amino-terminal domains of SREBPs that travel to the nucleus to up-regulate expression of lipogenic genes. The CTD of SREBP remains bound to Scap but must be eliminated so that Scap can be recycled to bind and transport additional SREBPs. Here, we provide insights into how this occurs by performing a detailed molecular dissection of the CTD of SREBP2, one of three SREBP isoforms expressed in mammals. We identify a degradation signal comprised of seven noncontiguous amino acids encoded in exon 19 that mediates SREBP2’s proteasomal degradation in the absence of Scap. When bound to the CTD of Scap, this signal is masked and SREBP2 is stabilized. Binding to Scap requires an arginine residue in exon 18 of SREBP2. After SREBP2 is cleaved in Golgi, its CTD remains bound to Scap and returns to the ER with Scap where it is eliminated by proteasomal degradation. The Scap-binding motif, but not the degradation signal, is conserved in SREBP1. SREBP1’s stability is determined by a degradation signal in a different region of its CTD. These findings highlight a previously unknown role for the CTD of SREBPs in regulating SREBP activity.

Haplotypes of bovine FoxO1 gene sequence variants and association with growth traits in Qinchuan cattle

2013 ◽  
Vol 93 (S2) ◽  
pp. 8-14 ◽  
Author(s):  
YUJIA SUN ◽  
JING XUE ◽  
WENJIAO GUO ◽  
MENJIAO LI ◽  
YONGZHEN HUANG ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Growth Traits ◽  
Sequence Variants ◽  
Qinchuan Cattle ◽  
Foxo1 Gene

scholarly journals Caspase-2, a Novel Lipid Sensor under the Control of Sterol Regulatory Element Binding Protein 2

2005 ◽  
Vol 25 (21) ◽  
pp. 9621-9631 ◽  
Author(s):  
E. Logette ◽  
C. Le Jossic-Corcos ◽  
D. Masson ◽  
S. Solier ◽  
A. Sequeira-Legrand ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Apoptotic Cell ◽  
Regulatory Element ◽  
Acid Synthesis ◽  
Lipid Lowering ◽  
Proteolytic Activation ◽  
Element Binding Protein ◽  
Sterol Regulatory Element ◽  
Caspase 2 ◽  
Interfering Rna

ABSTRACT Caspases play important roles in apoptotic cell death and in some other functions, such as cytokine maturation, inflammation, or differentiation. We show here that the 5′-flanking region of the human CASP-2 gene contains three functional response elements for sterol regulatory element binding proteins (SREBPs), proteins that mediate the transcriptional activation of genes involved in cholesterol, triacylglycerol, and fatty acid synthesis. Exposure of several human cell lines to statins, lipid-lowering drugs that drive SREBP proteolytic activation, induced the CASP-2 gene to an extent similar to that for known targets of SREBP proteins. Adenoviral vector-mediated transfer of active SREBP-2 also induced expression of the CASP-2 gene and the caspase-2 protein and increased the cholesterol and triacylglycerol cellular content. These rises in lipids were strongly impaired following small interfering RNA-mediated silencing of the CASP-2 gene. Taken together, our results identify the human CASP-2 gene as a member of the SREBP-responsive gene battery that senses lipid levels in cells and raise the possibility that caspase-2 participates in the control of cholesterol and triacylglycerol levels.

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