Novel gene rearrangements in the mitochondrial genome of a webspinner, Aposthonia japonica (Insecta: Embioptera)

Genome ◽  
2012 ◽  
Vol 55 (3) ◽  
pp. 222-233 ◽  
Author(s):  
Natuo Kômoto ◽  
Kenji Yukuhiro ◽  
Shuichiro Tomita
Keyword(s):  
Amino Acid ◽  
Phylogenetic Analyses ◽  
Nucleotide Composition ◽  
Amino Acid Sequences ◽  
Rrna Genes ◽  
Phylogenetic Position ◽  
12S Rrna ◽  
Rrna Gene ◽  
Protein Coding ◽  
Mtdna Sequence

Webspinners (order Embioptera) are polyneopteran insects characterized by enlarged foretarsi with silk glands, whose silk is used to produce galleries in which the insects live gregariously. The phylogenetic position of webspinners has been debated. In the present study, an almost complete mitochondrial DNA (mtDNA) sequence of Embioptera is reported for the first time. The mtDNA of a webspinner, Aposthonia japonica , has the 13 protein-coding genes (PCGs) generally found in metazoan mtDNA sequences. There is a translocation of a large region including atp6, atp8, cox3, nad3, and nad5 as well as a duplication of the 12S rRNA gene. The rearrangement does not seem to affect nucleotide composition, although amino acid composition in some parts of the mtDNA is biased compared with other Polyneoptera species. Based on phylogenetic analyses using nucleotide sequences of all PCGs concatenated with two rRNA genes and the amino acid sequences of all PCGs, A. japonica is sister to Verophasmatodea, a suborder of typical stick and leaf insects.

Characterization of the mitochondrial genome of Diphyllobothrium latum (Cestoda: Pseudophyllidea) – implications for the phylogeny of eucestodes

Parasitology ◽  
2006 ◽  
Vol 134 (5) ◽  
pp. 749-759 ◽  
Author(s):  
J.-K. PARK ◽  
K.-H. KIM ◽  
S. KANG ◽  
H. K. JEON ◽  
J.-H. KIM ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Phylogenetic Analyses ◽  
Complete Mitochondrial Genome ◽  
Amino Acid Sequences ◽  
Hymenolepis Diminuta ◽  
Rrna Genes ◽  
Gene Arrangement ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Diphyllobothrium Latum

SUMMARYThe complete nucleotide sequence of the mitochondrial genome was determined for the fish tapeworm Diphyllobothrium latum. This genome is 13 608 bp in length and encodes 12 protein-coding genes (but lacks the atp8), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes, corresponding to the gene complement found thus far in other flatworm mitochondrial (mt) DNAs. The gene arrangement of this pseudophyllidean cestode is the same as the 6 cyclophyllidean cestodes characterized to date, with only minor variation in structure among these other genomes; the relative position of trnS2 and trnL1 is switched in Hymenolepis diminuta. Phylogenetic analyses of the concatenated amino acid sequences for 12 protein-coding genes of all complete cestode mtDNAs confirmed taxonomic and previous phylogenetic assessments, with D. latum being a sister taxon to the cyclophyllideans. High nodal support and phylogenetic congruence between different methods suggest that mt genomes may be of utility in resolving ordinal relationships within the cestodes. All species of Diphyllobothrium infect fish-eating vertebrates, and D. latum commonly infects humans through the ingestion of raw, poorly cooked or pickled fish. The complete mitochondrial genome provides a wealth of genetic markers which could be useful for identifying different life-cycle stages and for investigating their population genetics, ecology and epidemiology.

scholarly journals Mitogenomics and the Phylogeny of Mantis Shrimps (Crustacea: Stomatopoda)

Diversity ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 647
Author(s):  
Cassandra Koga ◽  
Greg W. Rouse
Keyword(s):  
Sister Group ◽  
18S Rrna Gene ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Mitochondrial Genomes ◽  
12S Rrna ◽  
Rrna Gene ◽  
Slight Variation ◽  
Ancestral State ◽  
Protein Coding

Stomatopoda, commonly known as mantis shrimps, are notable for their enlarged second maxillipeds encompassing the raptorial claw. The form of the claw can be used to divide them into two basic groups: smashers and spearers. Previous phylogenetic studies of Stomatopoda have focused on morphology or a few genes, though there have been whole mitochondrial genomes published for 15 members of Stomatopoda. However, the sampling has been somewhat limited with key taxa not included. Here, nine additional stomatopod mitochondrial genomes were generated and combined with the other available mitogenomes for a phylogenetic analysis. We used the 13 protein coding genes, as well as 12S rRNA, 16S rRNA genes, and included nuclear 18S rRNA gene sequences. Different rooting options were used for the analyses: (1) single and multiple outgroups from various eumalocostracan relatives and (2) a stomatopod-only dataset, with Hemisquilla californiensis used to root the topologies, based on the current hypothesis that Hemisquilla is the sister group to the rest of Stomatopoda. The eumalocostracan-rooted analyses all showed H. californiensis nested within Stomatopoda, raising doubts as to previous hypotheses as to its placement. Allowing for the rooting difference, the H. californiensis outgroup datasets had the same tree topology as the eumalocostracan outgroup datasets with slight variation at poorly supported nodes. Of the major taxonomic groupings sampled to date, Squilloidea was generally found to be monophyletic while Gonodactyloidea was not. The position of H. californiensis was found inside its superfamily, Gonodactyloidea, and grouped in a weakly supported clade containing Odontodactylus havanensis and Lysiosquillina maculata for the eumalocostracan-rooted datasets. An ancestral state reconstruction was performed on the raptorial claw form and provides support that spearing is the ancestral state for extant Stomatopoda, with smashing evolving subsequently one or more times.

scholarly journals Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 107 ◽  
Author(s):  
Awais Ahmad ◽  
Xin Yang ◽  
Ting Zhang ◽  
Chunqun Wang ◽  
Caixian Zhou ◽  
...  
Keyword(s):  
Amino Acid ◽  
Complete Mitochondrial Genome ◽  
Small Ruminants ◽  
Complete Characterization ◽  
Amino Acid Sequences ◽  
Trna Genes ◽  
Protein Coding ◽  
Coding Regions ◽  
Mtdna Sequence ◽  
Mt Genome

The complete mitochondrial (mt) genome of Ostertagia trifurcata, a parasitic nematode of small ruminants, has been sequenced and its phylogenetic relationship with selected members from the superfamily Trichostrongyloidea was investigated on the basis of deduced datasets of mt amino acid sequences. The entire mt genome of Ostertagia trifurcata is circular and 14,151 bp in length. It consists of a total of 36 genes comprising 12 genes coding for proteins (PCGs), 2 genes for ribosomal RNA (rRNA), 22 transfer RNA (tRNA) genes and 2 non-coding regions, since all genes are transcribed in the same direction. The phylogenetic analysis based on the concatenated datasets of predicted amino acid sequences of the 12 protein coding genes supported monophylies of the Haemonchidae, Dictyocaulidae and Molineidae families, but rejected monophylies of the Trichostrongylidae family. The complete characterization and provision of the mtDNA sequence of Ostertagia trifurcata provides novel genetic markers for molecular epidemiological investigations, systematics, diagnostics and population genetics of Ostertagia trifurcata and its correspondents.

scholarly journals Characterization of the complete mitochondrial DNA of Theretra japonica and its phylogenetic position within the Sphingidae (Lepidoptera: Sphingidae)

ZooKeys ◽  
2018 ◽  
Vol 754 ◽  
pp. 127-139 ◽  
Author(s):  
Jun Li ◽  
Rui-Rui Lin ◽  
Yao-Yao Zhang ◽  
Kun-Jie Hu ◽  
Ya-Qi Zhao ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
Phylogenetic Position ◽  
Trna Genes ◽  
Rich Region ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Oxidase Subunit ◽  
Relationship Of

In the present study, the complete mitogenome of Theretrajaponica was sequenced and compared with other sequenced mitogenomes of Sphingidae species. The mitogenome of T.japonica, containing 37 genes (13 protein-coding genes, 22 tRNA genes, and two rRNA genes) and a region rich in adenine and thymine (AT-rich region), is a circular molecule with 15,399 base pairs (bp) in length. The order and orientation of the genes in the mitogenome are similar to those of other sequenced mitogenomes of Sphingidae species. All 13 protein-coding genes (PCGs) are initiated by ATN codons except for the cytochrome C oxidase subunit 1 gene (cox1) which is initiated by the codon CGA as observed in other lepidopteran insects. Cytochrome C oxidase subunit 2 gene (cox2) has the incomplete termination codon T and NADH dehydrogenase subunit 1 gene (nad1) terminates with TAG while the remainder terminates with TAA. Additionally, the codon distributions of the 13 PCGs revealed that Ile and Leu2 are the most frequently used codon families and codons CGG, CGC, CCG, CAG, and AGG are absent. The 431 bp AT-rich region includes the motif ATAGA followed by a 23 bp poly-T stretch, short tandem repeats (STRs) of TC and TA, two copies of a 28 bp repeat ‘ATTAAATTAATAAATTAA TATATTAATA’ and a poly-A element. Phylogenetic analyses within Sphingidae confirmed that T.japonica belongs to the Macroglossinae and showed that the phylogenetic relationship of T.japonica is closer to Ampelophagarubiginosa than Daphnisnerii. Phylogenetic analyses within Theretra demonstrate that T.japonica, T.jugurtha, T.suffusa, and T.capensis are clustered into one clade.

scholarly journals Complete mitochondrial genome of Whitmania laevis (Annelida, Hirudinea) and comparative analyses within Whitmania mitochondrial genomes

2020 ◽  
Vol 145 (2) ◽  
Author(s):  
Fei Ye ◽  
Ting Liu ◽  
Wenbo Zhu ◽  
Ping You
Keyword(s):  
Mitochondrial Genome ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Complete Mitochondrial Genome ◽  
Nucleotide Composition ◽  
Rrna Genes ◽  
Mitochondrial Genomes ◽  
Trna Genes ◽  
Protein Coding ◽  
Close Relationship

The complete mitochondrial genome of Whitmania laevis is 14,442 bp in length and contains 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The almost-complete mitochondrial genome of Whitmania acranulata, consisting of 13,494 bp, contains 35 genes including 13 PCGs, 20 tRNA genes, and two rRNA genes. COI phylogenetic analyses showed that the samples reported in GenBank and analysed as Hirudo nipponia KC667144, Hirudinaria manillensis KC688268 and Erpobdella octoculata KC688270 are not the named species and they should belong to Whitmania. We compared and analyzed the characteristics of nucleotide composition, codon usage, and secondary structures of 22 tRNAs and two rRNAs from Whitmania taxa. Moreover, we analyzed phylogenetic relationships of Annelida using maximum likelihood (ML) and Bayesian inference (BI) methods, based on 11 mitochondrial genes. Our results reveal that W. laevis has a close relationship with W. pigra.

scholarly journals Sequencing and characterization of mitochondrial DNA genome for Brama japonica (Perciformes: Bramidae) with phylogenetic consideration

2016 ◽  
Author(s):  
Fenfang Chen ◽  
Hongyu Ma ◽  
Chunyan Ma ◽  
Heng Zhang ◽  
Mengdi Zhao ◽  
...  
Keyword(s):  
Control Region ◽  
Complete Mitochondrial Genome ◽  
Nucleotide Composition ◽  
Start Codon ◽  
12S Rrna ◽  
Rrna Gene ◽  
Protein Coding ◽  
Conserved Sequence ◽  
Protein Coding Genes ◽  
Rna Genes

In the present study, we isolated and characterized the complete mitochondrial genome sequence of Brama japonica by polymerase chain reaction (PCR) amplification and primer-walking sequencing. The complete DNA was 17,009 bp in length and contained a typical set of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a long putative control region. The gene organization and nucleotide composition of complete mitogenome were identical to those of other Bramidae fishes. In contrast, the 12S rRNA gene contained a big poly C structure which was larger than those from other Bramidae species. Of 37 genes, twenty-eight were encoded by heavy strand, while nine were encoded by light strand. Among the 13 protein-coding genes, twelve employed ATG as start codon, while only COI utilized GTG as start codon. In the control region, the terminal associated sequence (TAS), the central and conserved sequence block (CSB-E and CSB-D) and a variable domain (CSB-1, CSB-2 and CSB-3) were identified, while the typical central conserved CSB-F could not be detected in B. japonica. The putative OL region can fold into a conserved secondary structure and the conserved motif (5’-GCCGG-3’) was found at the base of the stem in tRNACys. The overall nucleotide composition of this genome was 26.43% for A, 16.71% for G, 31.35% for C, and 25.50% for T, with a high A+T content of 51.93%. From the NJ phylogenetic tree, we can find that B. japonica was together with other five Bramidae species formed a monophyletic group among 24 species. This work provided a set of useful data for studying on population genetic diversity and molecular evolution in Bramidae and related fish species.

scholarly journals Phylogeny of Firmicutes with special reference to Mycoplasma (Mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data

2004 ◽  
Vol 54 (3) ◽  
pp. 871-875 ◽  
Author(s):  
Matthias Wolf ◽  
Tobias Müller ◽  
Thomas Dandekar ◽  
J. Dennis Pollack
Keyword(s):  
Amino Acid ◽  
Phylogenetic Trees ◽  
Gene Sequence ◽  
Sequence Data ◽  
Phosphoglycerate Kinase ◽  
Amino Acid Sequences ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Spiroplasma Citri ◽  
Mycoplasma Mycoides

The phylogenetic position of the Mollicutes has been re-examined by using phosphoglycerate kinase (Pgk) amino acid sequences. Hitherto unpublished sequences from Mycoplasma mycoides subsp. mycoides, Mycoplasma hyopneumoniae and Spiroplasma citri were included in the analysis. Phylogenetic trees based on Pgk data indicated a monophyletic origin for the Mollicutes within the Firmicutes, whereas Bacilli (Firmicutes) and Clostridia (Firmicutes) appeared to be paraphyletic. With two exceptions, i.e. Thermotoga (Thermotogae) and Fusobacterium (Fusobacteria), which clustered within the Firmicutes, comparative analyses show that at a low taxonomic level, the resolved phylogenetic relationships that were inferred from both the Pgk protein and 16S rRNA gene sequence data are congruent.

Comparative analyses of the complete mitochondrial genomes of Cyathostomum pateratum and Cyathostomum catinatum provide new molecular data for the evolution of Cyathostominae nematodes

2018 ◽  
Vol 93 (05) ◽  
pp. 643-647
Author(s):  
Y.Y. Qiu ◽  
M.H. Zeng ◽  
P.W. Diao ◽  
X.X. Wang ◽  
Q. Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Common Species ◽  
Molecular Data ◽  
Amino Acid Sequences ◽  
Rrna Genes ◽  
Parasitic Nematodes ◽  
Trna Genes ◽  
Conserved Sequence ◽  
Mtdna Sequence ◽  
Mt Genome

AbstractThe parasite Cyathostomum pateratum, which occurs in the large intestine of equines, is a common species of the subfamily Cyathostominae. Cyathostominae nematodes are a complex nematode group for which only limited genetic information has been reported. To re-examine the phylogenetic relationships among Cyathostominae nematodes, we sequenced the complete mitochondrial (mt) genome of Cy. pateratum and compared it with the mt genome of the congeneric species Cyathostomum catinatum. The complete mtDNA sequence of Cy. pateratum was 13,822 bp in length, 16 bp shorter than that of Cy. catinatum. The mtDNA sequences of both species contained 12 protein-coding genes, two rRNA genes and 22 tRNA genes, and all 36 genes were transcribed in the same direction and in the same strand. Pairwise comparisons of the 12 predicted amino acid sequences between Cy. catinatum and Cy. pateratum revealed differences of 0.4–3.1%; the least conserved sequence was that of cytochrome c oxidase subunit 3 (cox3). Phylogenetic analysis of the concatenated amino acid sequences using Bayesian inference and maximum likelihood methods showed that Cy. catinatum and Cy. pateratum clustered together with very high nodal support, and Cylicostephanus goldi was closer to the Cyathostomum nematodes than to other Cyathostominae nematodes. The mtDNA sequence of Cy. pateratum is reported here for the first time. The study will shed some light on the genetic evolution among parasitic nematodes in Cyathostomum.

Redescription and molecular characterisation of Xiphinema barense Lamberti et al., 1986 (Nematoda: Longidoridae) from wild olive trees in southern Italy

Nematology ◽  
2014 ◽  
Vol 16 (9) ◽  
pp. 1079-1089 ◽  
Author(s):  
Francesca De Luca ◽  
Antonio Archidona-Yuste ◽  
Alberto Troccoli ◽  
Elena Fanelli ◽  
Nicola Vovlas ◽  
...  
Keyword(s):  
Southern Italy ◽  
Phylogenetic Analyses ◽  
Original Description ◽  
Rrna Genes ◽  
Phylogenetic Position ◽  
Species Differentiation ◽  
Rrna Gene ◽  
28S Rrna ◽  
Wild Olive ◽  
Olive Trees

A population of Xiphinema barense from wild olive trees in Torre Pozzella, Brindisi province, southern Italy, is described using both morphological and molecular studies and compared with the description of the type specimens. The wild olive nematode population agrees very well with all morphometrics provided in the original description. However, detailed observations of the lumen of the tubular portion of the uterus in paratypes and specimens of the new population revealed a clear pseudo-Z-organ with small granules mixed with crystalloid bodies which were previously undetected. Photomicrographs of adult paratypes, which were lacking in the original description, and of specimens of the new population from wild olive trees are provided. The results of the phylogenetic analyses based on the sequences of the D2-D3 expansion regions of the 28S rRNA gene and ITS rRNA genes confirm the species differentiation and indicate the phylogenetic position of X. barense and its relationship with closely related species.

Resolving the phylogenetic position of the Wallemiomycetes: an enigmatic major lineage of Basidiomycota

2006 ◽  
Vol 84 (12) ◽  
pp. 1794-1805 ◽  
Author(s):  
P. Brandon Matheny ◽  
Jasmin A. Gossmann ◽  
Polona Zalar ◽  
T.K. Arun Kumar ◽  
David S. Hibbett
Keyword(s):  
Amino Acid ◽  
Ribosomal Rna ◽  
Gene Tree ◽  
Sister Group ◽  
Molecular Data ◽  
Amino Acid Sequences ◽  
Phylogenetic Position ◽  
Protein Coding ◽  
Branch Support ◽  
Robust Measures

The Wallemiomycetes includes three species of molds from the genus Wallemia . These fungi are adapted to environments of high osmotic stress, contaminate various foods, cause respiratory disease, and have an unusual mode of asexual reproduction. Wallemia was recently proposed as a new class based on 18S ribosomal RNA gene sequences to accommodate the isolated position of the clade in the Basidiomycota. We analyzed the phylogenetic position of the Wallemiomycetes using 3451 nucleotide characters of the 18S, 25S, and 5.8S ribosomal RNA genes and 1282 amino acid positions of rpb1, rpb2, and tef1 nuclear protein-coding genes across 91 taxa. Different gene regions and methods of phylogenetic inference produce mildly conflicting placements of the Wallemiomycetes. Parsimony analyses of nrDNA data suggest that the Wallemiomycetes is an early diverging lineage of Basidiomycota, occupying a basal position near the Entorrhizomycetidae. Ultrastructural data, some Bayesian analyses, and amino acid sequences suggest the Wallemiomycetes may be the sister group of the Agaricomycotina or Ustilaginomycotina. The combined gene tree supports the Wallemiomycetes as a lineage basal to a core clade of Pucciniomycotina, Ustilaginomycotina, and Agaricomycotina with robust measures of branch support. This study reinforces the isolated position of Wallemia in the Basidiomycota using molecular data from six nuclear genes. In total, five major lineages of Basidiomycota are recognized: the Agaricomycotina, Ustilaginomycotina, Pucciniomycotina, Entorrhizomycetidae, and the Wallemiomycetes.

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