Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 118-133 ◽  
Author(s):  
Margaret Gruber ◽  
Limin Wu ◽  
Matthew Links ◽  
Branimir Gjetvaj ◽  
Jonathan Durkin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Napus ◽  
Zinc Finger ◽  
Unknown Function ◽  
Flea Beetle ◽  
Gene Families ◽  
Feeding Damage ◽  
Brassica Napus L ◽  
Gene Encoding ◽  
Crucifer Flea Beetle

The molecular basis of canola ( Brassica napus L.) susceptibility to the crucifer flea beetle (FB, Phyllotreta cruciferae Goeze) was investigated by comparing transcript representation in FB-damaged and undamaged cotyledons. The B. napus cotyledon transcriptome increased and diversified substantially after FB feeding damage. Twenty-two genes encoding proteins with unknown function, six encoding proteins involved in signaling, and a gene encoding a B-box zinc finger transcription factor were moderately or strongly changed in representation with FB feeding damage. Zinc finger and calcium-dependent genes formed the largest portion of transcription factors and signaling factors with changes in representation. Six genes with unknown function, one transcription factor, and one signaling gene specific to the FB-damaged library were co-represented in a FB-damaged leaf library. Out of 188 transcription factor and signaling gene families screened for “early” expression changes, 16 showed changes in expression within 8 h. Four of these early factors were zinc finger genes with representation only in the FB-damaged cotyledon. These genes are now available to test their potential at initiating or specifying cotyledon responses to crucifer FB feeding.

Susceptibility of inbred lines of oilseed rape, Brassica napus, to feeding damage by the crucifer flea beetle, Phyllotreta cruciferae (Goeze) [Coleoptera: Chrysomelidae], and its inheritance

1993 ◽  
Vol 73 (2) ◽  
pp. 615-623 ◽  
Author(s):  
R. J. Lamb ◽  
P. Palaniswamy ◽  
R. P. Bodnaryk ◽  
P. B. E. McVetty ◽  
S. E. Jeong
Keyword(s):  
Brassica Napus ◽  
Genetic Basis ◽  
Flea Beetle ◽  
Inbred Lines ◽  
Brassica Napus L ◽  
Phyllotreta Cruciferae ◽  
Endosperm Proteins ◽  
Damage Level ◽  
Crucifer Flea Beetle ◽  
Resistant Genotypes

Seventy-seven inbred lines of Brassica napus L. were assessed for antixenosis to damage by die crucifer flea beetle, Phyllotreta cruciferae (Goeze). The resistance was quantified by measuring the level of damage inflicted on seedlings of each line in laboratory tests. One of these lines, M12, was more susceptible than another line, L19, in a series of replicated tests, but individual seedlings could not be identified as resistant or susceptible because inter-seedling variation in the damage level was high. The F3 families from reciprocal crosses between L19 and M12 showed segregation for the resistance, demonstrating that the antixenosis has a genetic basis. Two or more genes probably control the expression of the resistance, but the number could not be estimated. Electrophoresis of seed endosperm proteins revealed a band, P-74, that occurred in M12 and eight cultivars of B. napus, but not in L19. This banding pattern was inherited as a simple recessive allele, but it proved not to be linked with the resistance in the F3 families. Further screening of B. napus for highly resistant genotypes and identification of linked genetic markers are needed to establish agronomically useful levels of flea beetle resistance in this crop. Key words: Insecta, resistance, canola, electrophoresis, endosperm proteins

When is fall feeding by flea beetles (Phyllotreta spp., Coleoptera: Chrysomelidae) on canola (Brassica napus L.) a problem?

2012 ◽  
Vol 92 (1) ◽  
pp. 97-107
Author(s):  
Juliana J. Soroka ◽  
Larry F. Grenkow
Keyword(s):  
Brassica Napus ◽  
Field Experiments ◽  
Flea Beetle ◽  
Brassica Napus L ◽  
Late Season ◽  
The Third ◽  
Flea Beetles ◽  
Seeding Date ◽  
Seed Yields

Soroka, J. J. and L. F. Grenkow. 2012. When is fall feeding by flea beetles ( Phyllotreta spp., Coleoptera: Chrysomelidae) on canola ( Brassica napus L.) a problem? Can. J. Plant Sci. 92: 97–107. Two cultivars of Brassica napus canola were seeded in mid-May and early June in three field experiments in each of 3 yr near Saskatoon, Saskatchewan, to determine the effects of late-season flea beetle feeding on seed yields. In the first experiment, canola was sprayed with insecticide late in the summer to eliminate naturally-infesting flea beetles. In the second, 1×1×1.5 m screen cages were placed over early- and late-seeded canola at flowering and infested with flea beetles as canola matured. In the third investigation, sleeve cages were placed over individual plants and infested with 100 flea beetles. Flea beetles had no detrimental effects on early-seeded canola in any experiment, but did affect seed yields of late-seeded plots in some trials. Over two cultivars in 1 year, late-seeded plants in cube cages infested with about 350 flea beetles per plant when lower pods were turning from translucent to green in colour reduced yield by 241 kg ha−1 over control yields. Seed weights in these late-seeded plots were decreased from 2.68 g per 1000 seeds in uninfested cages to 2.44 g per 1000 seeds in infested cages. Populations of 100 flea beetles per plant in sleeve cages had no effect on harvest parameters in any seeding date or year.

scholarly journals Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

2016 ◽  
Vol 44 (2) ◽  
pp. 435-444
Author(s):  
Imen GLAIED GHRAM ◽  
Hatem BELGUITH ◽  
Maha BEN MUSTAPHA ◽  
Issam HIMILA ◽  
Balkiss BOUHAOUALA ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Expression Patterns ◽  
Pcr Amplification ◽  
Gene Families ◽  
Lipase Gene ◽  
Homologous Proteins ◽  
Brassica Napus L ◽  
Calculated Molecular Mass ◽  
Hydrolysis Of

Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of Brassica napus genomic DNA, with oligonucleotide primers derived from the sequence of an Arabidopsis thaliana homologue. The clone included an open reading frame of 1581 bp encoding a polypeptide of 526 amino acids, with a calculated molecular mass of 59.5 kDa. Analysis of the deduced protein sequence, sequence alignment with homologous proteins from related plant species, and a phylogenetic analysis revealed that the BnLIP protein belongs to the ‘classical’ GxSxG-motif lipase family. RT-PCR assays indicated that the BnLIP gene is expressed specifically, but only transiently, during seed germination: the lipase mRNA was not present at detectable levels in ungerminated seeds, was detected only three days after seed imbibition, but its levels decreased rapidly afterwards. No expression was observed in roots, stems or leaves of adult plants. This expression pattern suggests that BnLIP is one of the lipases involved in the hydrolysis of triacylglycerides stored in rapeseed seeds, ultimately providing nutrients and energy to sustain seedling growth until photosynthesis is activated.

Tricho-Rhino-Phalangeal Syndrome with Supernumerary Teeth

2008 ◽  
Vol 87 (11) ◽  
pp. 1027-1031 ◽  
Author(s):  
P. Kantaputra ◽  
I. Miletich ◽  
H.-J. Lüdecke ◽  
E.Y. Suzuki ◽  
V. Praphanphoj ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Amino Acid ◽  
Zinc Finger ◽  
Clinical Features ◽  
Tooth Development ◽  
Supernumerary Teeth ◽  
Gene Encoding ◽  
Gata Transcription Factor ◽  
Craniofacial Defects

Tricho-rhino-phalangeal syndromes (TRPS) are caused by mutation or deletion of TRPS1, a gene encoding a GATA transcription factor. These disorders are characterized by abnormalities of the hair, face, and selected bones. Rare cases of individuals with TRPS displaying supernumerary teeth have been reported, but none of these has been examined molecularly. We used two different approaches to investigate a possible role of TRPS1 during tooth development. We looked at the expression of Tprs1 during mouse tooth development and analyzed the craniofacial defects of Trps1 mutant mice. In parallel, we investigated whether a 17-year-old Thai boy with clinical features of TRPS and 5 supernumerary teeth had mutation in TRPS1. We report here that Trps1 is expressed during mouse tooth development, and that an individual with TRPS with supernumerary teeth has the amino acid substitution A919V in the GATA zinc finger of TRPS1. These results suggest a role for TRPS1 in tooth morphogenesis.

Identification and characterization of NF-Y transcription factor families in Canola (Brassica napus L.)

Planta ◽  
2013 ◽  
Vol 239 (1) ◽  
pp. 107-126 ◽  
Author(s):  
Mingxiang Liang ◽  
Xiangzhen Yin ◽  
Zhongyuan Lin ◽  
Qingsong Zheng ◽  
Guohong Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Napus ◽  
Brassica Napus L ◽  
Identification And Characterization

scholarly journals Systematic Analysis of the DNA Methylase and Demethylase Gene Families in Rapeseed (Brassica napus L.) and Their Expression Variations After Salt and Heat stresses

2020 ◽  
Vol 21 (3) ◽  
pp. 953 ◽  
Author(s):  
Shihang Fan ◽  
Hongfang Liu ◽  
Jing Liu ◽  
Wei Hua ◽  
Shouming Xu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Brassica Napus ◽  
Stress Responses ◽  
Methylation Status ◽  
Functional Characterization ◽  
Gene Families ◽  
Brassica Napus L ◽  
Systematic Analysis ◽  
Dna Methylase ◽  
Dna Demethylase

DNA methylation is a process through which methyl groups are added to the DNA molecule, thereby modifying the activity of a DNA segment without changing the sequence. Increasing evidence has shown that DNA methylation is involved in various aspects of plant growth and development via a number of key processes including genomic imprinting and repression of transposable elements. DNA methylase and demethylase are two crucial enzymes that play significant roles in dynamically maintaining genome DNA methylation status in plants. In this work, 22 DNA methylase genes and six DNA demethylase genes were identified in rapeseed (Brassica napus L.) genome. These DNA methylase and DNA demethylase genes can be classified into four (BnaCMTs, BnaMET1s, BnaDRMs and BnaDNMT2s) and three (BnaDMEs, BnaDML3s and BnaROS1s) subfamilies, respectively. Further analysis of gene structure and conserved domains showed that each sub-class is highly conserved between rapeseed and Arabidopsis. Expression analysis conducted by RNA-seq as well as qRT-PCR suggested that these DNA methylation/demethylation-related genes may be involved in the heat/salt stress responses in rapeseed. Taken together, our findings may provide valuable information for future functional characterization of these two types of epigenetic regulatory enzymes in polyploid species such as rapeseed, as well as for analyzing their evolutionary relationships within the plant kingdom.

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