scholarly journals High resolution linkage maps of the model organism Petunia reveal substantial synteny decay with the related genome of tomato

Genome ◽  
2011 ◽  
Vol 54 (4) ◽  
pp. 327-340 ◽  
Author(s):  
Eligio Bossolini ◽  
Ulrich Klahre ◽  
Anna Brandenburg ◽  
Didier Reinhardt ◽  
Cris Kuhlemeier
Keyword(s):  
Fragment Length Polymorphism ◽  
Genetic Characterization ◽  
Model Organism ◽  
Linkage Maps ◽  
Related Genome ◽  
Petunia Inflata ◽  
First Time ◽  
Mapping Populations ◽  
Petunia Axillaris

Two linkage maps were constructed for the model plant Petunia. Mapping populations were obtained by crossing the wild species Petunia axillaris subsp. axillaris with Petunia inflata, and Petunia axillaris subsp. parodii with Petunia exserta. Both maps cover the seven chromosomes of Petunia, and span 970 centimorgans (cM) and 700 cM of the genomes, respectively. In total, 207 markers were mapped. Of these, 28 are multilocus amplified fragment length polymorphism (AFLP) markers and 179 are gene-derived markers. For the first time we report on the development and mapping of 83 Petunia microsatellites. The two maps retain the same marker order, but display significant differences of recombination frequencies at orthologous mapping intervals. A complex pattern of genomic rearrangements was detected with the related genome of tomato (Solanum lycopersicum), indicating that synteny between Petunia and other Solanaceae crops has been considerably disrupted. The newly developed markers will facilitate the genetic characterization of mutants and ecological studies on genetic diversity and speciation within the genus Petunia. The maps will provide a powerful tool to link genetic and genomic information and will be useful to support sequence assembly of the Petunia genome.

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

scholarly journals Natural Infection of Southern Highbush Blueberry (Vaccinium corymbosum Interspecific Hybrids) by Xylella fastidiosa subsp. fastidiosa

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2598-2605
Author(s):  
Dario Di Genova ◽  
Kippy J. Lewis ◽  
Jonathan E. Oliver
Keyword(s):  
Genetic Characterization ◽  
Xylella Fastidiosa ◽  
Natural Infection ◽  
Vaccinium Corymbosum ◽  
Significant Similarity ◽  
Potential Impact ◽  
Southern Highbush ◽  
Southern Georgia ◽  
First Time

Xylella fastidiosa (Xf) is an emerging insect-vectored, xylem-limited bacterium that can cause disease on several economically important fruit and tree crops including almond, blueberry, citrus, grapevine, peach, and pecan. On blueberry, Xf causes bacterial leaf scorch (BLS), which is prevalent in the southeastern United States. This disease, previously reported to be caused by Xf subsp. multiplex (Xfm), can result in rapid plant decline and death of southern highbush (SHB) blueberry cultivars. In 2017, a survey of blueberry plantings in southern Georgia (U.S.A.) confirmed the presence of Xf-infected plants in eight of nine sites examined, and seven isolates were cultured from infected plants. Genetic characterization of these isolates through single-locus and multilocus sequence analysis revealed that three isolates from two sites belonged to Xf subsp. fastidiosa (Xff), with significant similarity to isolates from grapevine. After these three isolates were artificially inoculated onto greenhouse-grown SHB blueberries (cv. ‘Rebel’), symptoms typical of BLS developed, and Xff infection was confirmed through genetic characterization and reisolation of the bacterium to fulfill Koch’s postulates. Because all previously reported Xf isolates from blueberry have been characterized as Xfm, this is the first time that isolation of Xff has been reported from naturally infected blueberry plantings. The potential impact of Xff isolates on disease management in blueberry requires further exploration. Furthermore, given that isolates from both Xfm and Xff were obtained within a single naturally infected blueberry planting, blueberry in southern Georgia may provide opportunities for intersubspecific recombination between Xff and Xfm isolates.

scholarly journals Molecular Characterization of New Natural Hybrids of Saccharomyces cerevisiae and S. kudriavzevii in Brewing

2008 ◽  
Vol 74 (8) ◽  
pp. 2314-2320 ◽  
Author(s):  
Sara S. González ◽  
Eladio Barrio ◽  
Amparo Querol
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Natural Hybrids ◽  
New Type ◽  
Saccharomyces Kudriavzevii ◽  
Different Origins ◽  
First Time ◽  
Restriction Fragment Length

ABSTRACT We analyzed 24 beer strains from different origins by using PCR-restriction fragment length polymorphism analysis of different gene regions, and six new Saccharomyces cerevisiae × Saccharomyces kudriavzevii hybrid strains were found. This is the first time that the presence in brewing of this new type of hybrid has been demonstrated. From the comparative molecular analysis of these natural hybrids with respect to those described in wines, it can be concluded that these originated from at least two hybridization events and that some brewing hybrids share a common origin with wine hybrids. Finally, a reduction of the S. kudriavzevii fraction of the hybrid genomes was observed, but this reduction was found to vary among hybrids regardless of the source of isolation. The fact that 25% of the strains analyzed were discovered to be S. cerevisiae × S. kudriavzevii hybrids suggests that an important fraction of brewing strains classified as S. cerevisiae may correspond to hybrids, contributing to the complexity of Saccharomyces diversity in brewing environments. The present study raises new questions about the prevalence of these new hybrids in brewing as well as their contribution to the properties of the final product.

scholarly journals Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of Theileria haneyi in Egypt

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1414
Author(s):  
Bassma S. M. Elsawy ◽  
Ahmed M. Nassar ◽  
Heba F. Alzan ◽  
Raksha V. Bhoora ◽  
Sezayi Ozubek ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genetic Characterization ◽  
Simultaneous Detection ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Pcr Targeting ◽  
First Time

Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of T. equi and B. caballi for the simultaneous detection of EP in Egyptian equids and examined the presence of T. haneyi infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting T. hayeni. The mPCR method revealed a prevalence of T. equi of 20.3% in horses and of 13.1% in donkeys and a prevalence of B. caballi of 1.2% in horses. B. caballi was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of T. haneyi in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with T. haneyi and T. equi was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.

scholarly journals Plumularioid hydroids (Cnidaria: Hydrozoa) from off New Caledonia collected during KANACONO and KANADEEP expeditions of the French Tropical Deep-Sea Benthos Program

2020 ◽  
Author(s):  
Horia R. Galea ◽  
Davide Maggioni
Keyword(s):  
Deep Sea ◽  
Genetic Characterization ◽  
New Caledonia ◽  
Valid Species ◽  
Western Coast ◽  
New Genera ◽  
Phylogenetic Reconstructions ◽  
The Family ◽  
First Time

This study reports on 25 species of hydroids occurring in the collections gathered during KANACONO and KANADEEP expeditions carried out in the SE of New Caledonia in 2016, and off the western coast of the island in 2017, respectively. Of these, 19 have not been dealt with in earlier reports on these collections. Two new genera and four new species are described, viz, Actinopluma mirifica Galea gen. et sp. nov., provisionally assigned to the family Kirchenpaueriidae Stechow, 1921, Schizoplumularia helicoidalis sp. nov., belonging to the Plumulariidae McCrady, 1859, and Corhiza patula Galea sp. nov. and Thamnopteros uniserius Galea gen. et sp. nov., both placed in the family Halopterididae Millard, 1962. The gonotheca and the medusoid gonophore of Plumularia contraria Ansín Agís et al., 2014 are described for the first time, allowing a genus transfer to Dentitheca Stechow, 1919, as D. contraria comb. nov. Plumularia conjuncta Billard, 1913, known earlier from a minute portion of colony, is redescribed based on a complete, though infertile, specimen. Similarly, complete specimens corresponding to the hydroid previously referred to as Antennella megatheca Ansín Agís et al., 2009 are documented, allowing a provisional reallocation to Corhiza Millard, 1962 and a description of its so far unknown gonothecae. Fertile material assignable to the poorly-known Monostaechas fisheri Nutting, 1905 allows the recognition of this hydroid as a valid species, distinct from M. quadridens (McCrady, 1859). Most taxa are illustrated to validate the reliability of their identifications. Finally, phylogenetic reconstructions of the families Aglaopheniidae, Plumulariidae, and Halopterididae, based on the 16S rRNA, allowed a first genetic characterization of some of the species dealt with in this work.

scholarly journals A Physiological Characterization in Controlled Bioreactors Reveals a Novel Survival Strategy for Debaryomyces hansenii at High Salinity and Confirms its Halophilic Behavior

2020 ◽  
Author(s):  
Clara Navarrete ◽  
August T. Frost ◽  
Laura Ramos-Moreno ◽  
Mette R. Krum ◽  
José L. Martínez
Keyword(s):  
Carbon Metabolism ◽  
Debaryomyces Hansenii ◽  
Model Organism ◽  
Central Carbon Metabolism ◽  
Culture Conditions ◽  
Physiological Characterization ◽  
Behavioural Patterns ◽  
Central Carbon ◽  
First Time

AbstractDebaryomyces hansenii is traditionally described as a halotolerant non-conventional yeast, being the model organism for the study of osmo- and salt tolerance mechanisms in eukaryotic systems for the past 30 years.However, unravelling of D. hansenii’s biotechnological potential has always been difficult due to the persistent limitations in the availability of efficient molecular tools described for this yeast. Additionally, there is a lack of consensus and contradictory information along the recent years that limits a comprehensive understanding of its central carbon metabolism, mainly due to a lack of physiological studies in controlled and monitored environments. Moreover, there is controversy about the diversity in the culture conditions (media composition, temperature and pH among others) used by different groups, which makes it complicated when trying to get significant conclusions and behavioural patterns.In this work, we present for the first time a physiological characterization of D. hansenii in batch cultivations using highly instrumented and controlled lab-scale bioreactors. Our findings contribute to a more complete picture of the central carbon metabolism and the external pH influence on the yeast ability to tolerate high Na+ and K+ concentrations. Finally, the controversial halophilic/halotolerant character of this yeast is further clarified.

Sign in / Sign up

Export Citation Format

Share Document