QTLs for important breeding characteristics in the doubled haploid oat progeny

Genome ◽  
2010 ◽  
Vol 53 (6) ◽  
pp. 482-493 ◽  
Author(s):  
Pirjo Tanhuanpää ◽  
Outi Manninen ◽  
Elina Kiviharju
Keyword(s):  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Doubled Haploid ◽  
Avena Sativa ◽  
Quantitative Trait ◽  
Oil Content ◽  
Mapping Population ◽  
Field Trials ◽  
Quality Traits ◽  
Leaf Blotch

A homozygous mapping population, consisting of doubled haploid (DH) oat ( Avena sativa L.) plants generated through anther culture of F1 plants from the cross between the Finnish cultivar ‘Aslak’ and the Swedish cultivar ‘Matilda’, was used to construct an oat linkage map. Ten agronomic and quality traits were analyzed in the DH plants from field trials in 2005 and 2006. Leaf blotch (caused by Pyrenophora avenae ) resistance was also evaluated in a greenhouse test with 2 different isolates. One to 8 quantitative trait loci (QTLs) were found to be associated with each trait studied. Some chromosomal regions affected more than 1 trait; for example, 4 regions affected both protein and oil content. This study gives valuable information to oat breeders concerning the inheritance of important traits, and it provides potential tools to assist breeding.

An updated doubled haploid oat linkage map and QTL mapping of agronomic and grain quality traits from Canadian field trials

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 289-301 ◽  
Author(s):  
Pirjo Tanhuanpää ◽  
Outi Manninen ◽  
Aaron Beattie ◽  
Peter Eckstein ◽  
Graham Scoles ◽  
...  
Keyword(s):  
Linkage Map ◽  
Doubled Haploid ◽  
Avena Sativa ◽  
Dna Markers ◽  
Grain Quality ◽  
Field Trials ◽  
Diversity Array Technology ◽  
Linkage Groups ◽  
Array Technology ◽  
Reference Map

The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 important breeding traits analyzed from Finnish and Canadian field trials. The new markers enabled most of the linkage groups to be anchored to the ‘Kanota’ × ‘Ogle’ oat ( Avena sativa L.) reference map and allowed comparison of the QTLs located in this study with those found previously. Two to 12 QTLs for each trait were discovered, of which several were expressed consistently across several environments.

Genes and Quantitative Trait Loci Controlling Biomass Yield and Forage Quality Traits in Perennial Wildrye

Crop Science ◽  
2014 ◽  
Vol 54 (1) ◽  
pp. 111-126 ◽  
Author(s):  
Steven R. Larson ◽  
Kevin B. Jensen ◽  
Joseph G. Robins ◽  
Blair L. Waldron
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Biomass Yield ◽  
Forage Quality ◽  
Quality Traits ◽  
Trait Loci

scholarly journals Novel Quantitative Trait Loci for Grain Cadmium Content Identified in Hard White Spring Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Ling Qiao ◽  
Justin Wheeler ◽  
Rui Wang ◽  
Kyle Isham ◽  
Natalie Klassen ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Candidate Gene ◽  
Spring Wheat ◽  
Quantitative Trait ◽  
Field Trials ◽  
Cadmium Content ◽  
Wheat Population ◽  
Trait Loci ◽  
Multiple Field ◽  
First Time

Cadmium (Cd) is a heavy metal that can cause a variety of adverse effects on human health, including cancer. Wheat comprises approximately 20% of the human diet worldwide; therefore, reducing the concentrations of Cd in wheat grain will have significant impacts on the intake of Cd in food products. The tests for measuring the Cd content in grain are costly, and the content is affected significantly by soil pH. To facilitate breeding for low Cd content, this study sought to identify quantitative trait loci (QTL) and associated molecular markers that can be used in molecular breeding. One spring wheat population of 181 doubled haploid lines (DHLs), which was derived from a cross between two hard white spring wheat cultivars “UI Platinum” (UIP) and “LCS Star” (LCS), was assessed for the Cd content in grain in multiple field trials in Southeast Idaho, United States. Three major QTL regions, namely, QCd.uia2-5B, QCd.uia2-7B, and QCd.uia2-7D, were identified on chromosomes 5B, 7B, and 7D, respectively. All genes in these three QTL regions were identified from the NCBI database. However, three genes related to the uptake and transport of Cd were used in the candidate gene analysis. The sequences of TraesCS5B02G388000 (TaHMA3) in the QCd.uia2-5B region and TraesCS7B02G320900 (TaHMA2) and TraesCS7B02G322900 (TaMSRMK3) in the QCd.uia2-7B region were compared between UIP and LCS. TaHMA2 on 7B is proposed for the first time as a candidate gene for grain Cd content in wheat. A KASP marker associated with this gene was developed and it will be further validated in near-isogenic lines via a gene-editing system in future studies.

Sign in / Sign up

Export Citation Format

Share Document