Development of molecular markers and linkage maps for the Carthamus species C. tinctorius and C. oxyacanthus

Genome ◽  
2010 ◽  
Vol 53 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Reinhold Mayerhofer ◽  
Catherine Archibald ◽  
Victoria Bowles ◽  
Allen G. Good
Keyword(s):  
Linkage Group ◽  
Flower Color ◽  
Carthamus Tinctorius ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Molecular Tools ◽  
Genomic Libraries ◽  
Inversion Event ◽  
Common Parent ◽  
Two Populations

A set of SSR and RFLP markers for safflower ( Carthamus tinctorius ) and jeweled distaff thistle ( C. oxyacanthus ) was generated from cDNA and genomic libraries and by mining public and proprietary sequence databases. In total, 1412 PCR-based markers and 75 RFLP markers were screened and polymorphic loci were mapped in an intraspecific F2 population of C. tinctorius and an interspecific BC1 population of C. tinctorius × C. oxyacanthus. The two populations shared one common parent and the resulting linkage maps could be compared for synteny. The level of polymorphism was low in both populations and only 8.2% and 13.7% of the analyzed markers could be mapped in the intraspecific and interspecific maps, respectively. The two maps showed significant colinearity of markers in several regions and an apparent translocation or inversion event on one linkage group. Noticeable segregation distortion was found on one linkage group of the C. tinctorius map and dense clustering of loci occurred on several linkage groups of the C. oxyacanthus map. The two maps represent the first major linkage analysis of Carthamus species. The molecular tools will be useful for a variety of genetic and genomic applications in safflower and its related species and have been used in our laboratory to map a flower color gene in C. tinctorius.

Two genetic linkage maps of mungbean using RFLP and RAPD markers

2000 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
C. J. Lambrides ◽  
R. J. Lawn ◽  
I. D. Godwin ◽  
J. Manners ◽  
B. C. Imrie
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

scholarly journals Quantitative Trait Loci (QTL) Analysis of Canning Quality Traits in Kidney Bean (Phaseolus vulgaris L.)

2002 ◽  
Vol 127 (4) ◽  
pp. 608-615 ◽  
Author(s):  
Maria-Carmela T. Posa-Macalincag ◽  
George L. Hosfield ◽  
Kenneth F. Grafton ◽  
Mark A. Uebersax ◽  
James D. Kelly
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Group ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Recombinant Inbred Lines ◽  
Kidney Bean ◽  
Major Genes ◽  
The Core ◽  
Two Populations ◽  
Canning Quality

Canning quality of dry bean (Phaseolus vulgaris L.), of which the degree of splitting (SPLT) and overall appearance (APP) of canned beans are major components, is a complex trait that exhibits quantitative inheritance. The objectives of this study were to identify major genes that affect APP and SPLT in kidney bean, and map the location of these loci to the integrated core map of common bean. The analysis was performed using random amplified polymorphic DNA (RAPD) markers and two populations of kidney bean, consisting of 75 and 73 recombinant inbred lines (RILs), respectively. The two populations—`Montcalm' × `California Dark Red Kidney 82' and `Montcalm' × `California Early Light Red Kidney'—were planted in six year-location combinations in Michigan, Minnesota and North Dakota from 1996 to 1999. Correlations between APP and SPLT were high (0.91 to 0.97). Heritability estimates for APP and SPLT ranged from 0.83 to 0.85 in the two populations. Major genes for these traits were identified on two linkage groups. The first QTL, associated with seven RAPD markers, was putatively mapped to the B8 linkage group of the core bean linkage map. Desirable canning quality appeared to be derived from Montcalm at this locus. The second QTL, associated with four markers, appeared to be derived from the California parents. The second linkage group was not assigned to a linkage group in the core map. Population and environment-specificity were observed for the markers identified.

Genetics of Glossina morsitans morsitans (Diptera: Glossinidae). XIII. Mapping the locus for tetrazolium oxidase in linkage group I and refinement of the linkage group II map

Genome ◽  
1988 ◽  
Vol 30 (6) ◽  
pp. 885-887 ◽  
Author(s):  
R. H. Gooding ◽  
B. M. Rolseth ◽  
S. A. Tarimo
Keyword(s):  
Linkage Group ◽  
Key Words ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Linkage Maps ◽  
Glossina Morsitans ◽  
Eye Color ◽  
Group I ◽  
Group Ii

The locus for tetrazolium oxidase, To, is mapped at 4.3 ± 1.3 recombination units from the locus for arginine phosphokinase, Apk, in linkage group I, and the distance between the eye color locus, sal, and Apk is confirmed to be about 39.5 ± 3.2 recombination units. In linkage group II the loci for aldehyde oxidase, Ao, and for two esterases are arranged in the order Ao Est-1 Est-2 with 3.5 ± 1.2 recombination units separating Ao and Est-1 and 8.3 ± 1.8 recombination units separating Est-1 and Est-2.Key words: Glossina morsitans, tetrazolium oxidase, aldehyde oxidase, esterases, linkage maps.

Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28)

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 626-629 ◽  
Author(s):  
Yuji Yasukochi ◽  
Yutaka Banno ◽  
Kohji Yamamoto ◽  
Marian R Goldsmith ◽  
Hiroshi Fujii
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Analysis ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Linkage Maps ◽  
Wild Type ◽  
Linkage Groups ◽  
Sequence Tagged Sites ◽  
Silkworm Bombyx Mori

Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was carried out using BF1 progeny between wild-type and mutant stocks carrying morphological phenotypic markers. As a result, the counterparts for 26 of 28 molecular LGs were identified with their counterparts of the classical LGs. Two visible markers, Sel and Xan, representing different classical LGs, were found to be linked.Key words: Bombyx mori, classical linkage group (LG), PCR-based genotyping, mutant, STS.

Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Genome ◽  
2014 ◽  
Vol 57 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Yang Yang ◽  
Benjamin D. Speth ◽  
Napatsakorn Boonyoo ◽  
Eric Baumert ◽  
Taylor R. Atkinson ◽  
...  
Keyword(s):  
Linkage Group ◽  
Male Sterility ◽  
Genetic Linkage ◽  
Chromosome 2 ◽  
Linkage Maps ◽  
Male Sterile ◽  
Molecular Linkage Group ◽  
Sterile Female ◽  
Genetic Linkage Maps ◽  
Sterility Genes

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

Interspecific nuclear–cytoplasmic compatibility controlled by genes on group 1 chromosomes in durum wheat

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 803-808 ◽  
Author(s):  
J. A. Anderson ◽  
S. S. Maan
Keyword(s):  
Dna Markers ◽  
Male Fertility ◽  
Spontaneous Mutation ◽  
Nuclear Genome ◽  
Rflp Markers ◽  
Seedling Vigor ◽  
Group 1 Chromosomes ◽  
Two Populations ◽  
Aneuploid Analysis ◽  
Group 1

Triticum longissimum cytoplasm is incompatible with the T. turgidum nuclear genome. Two nuclear genes, scs and Vi, derived from the nuclear genome of T. timopheevii and by a spontaneous mutation, respectively, restore nuclear–cytoplasmic compatibility, normal plant vigor, and male fertility in these alloplasmic genotypes. The objectives of this study were (i) to determine the chromosomal locations of scs and Vi; (ii) to identify DNA markers for scs and Vi; and (iii) to determine the interactions involving the dosage of scs and Vi. Two populations segregating for scs and Vi were produced and scored for seedling vigor (indicating presence of scs) and degree of self-fertility (indicating presence of Vi). Four RFLP markers were mapped near scs. Aneuploid analysis revealed that these markers, and hence the scs gene, are located on the long arm of chromosome 1A. Four RFLP markers were mapped near Vi on 1BS. Results indicated that other factors may be inhibiting the expression of Vi. We determined the dosage of scs and Vi in both populations with the aid of the linked RFLP markers. Individuals with two versus one dose of scs had reduced self-fertility, while individuals with two versus one dose of Vi had similar self-fertility.Key words: scs, Vi, Triticum, nucleocytoplasmic compatibility, RFLP.

Marker-assisted Selection for Improved Emergence and Eating Quality in Sweet Corn

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 472c-472
Author(s):  
Gad G. Yousef ◽  
John A. Juvik
Keyword(s):  
Complex Traits ◽  
Marker Assisted Selection ◽  
Sweet Corn ◽  
Phenotypic Selection ◽  
Rflp Markers ◽  
Eating Quality ◽  
Gene Marker ◽  
Stand Establishment ◽  
Selection For ◽  
Two Populations

Marker-assisted selection is proposed to be more effective than phenotypic selection in improving complex traits with low heritability. This study was designed to test empirically the efficiency of marker-assisted selection (MAS) vs. phenotypic selection (PS) in enhancing economically important characters in sweet corn using composite populations consisting of selected F2:3 families from two populations. In previous studies in our laboratory, these segregating populations were developed and assayed for genes influencing sweet corn emergence and eating quality. The 214 F2:3 families in the first population (W678su1 × IL731ase1) were classified into three sub-populations according to segregation for the se1gene. Marker-assisted selection and phenotypic selection were applied on the two homozygous sugary1 and sugary enhancer1 sub-populations separately. The second population consisted of 117 F2:3 families from the cross of Ia453sh2 × IL451bsh2. The genotypic selection was based on the polymorphism of five RFLP markers linked to QTL associated with significant effects on emergence and eating quality in the F2:3 generation. Twenty percent of the families in each population with the highest and lowest genotypic scores and phenotypic performance values were selected to constitute the MAS and PS composites, respectively. Emergence was evaluated in four different environments in Illinois and Wisconsin, while eating quality traits were evaluated in Illinois only. Results for emergence, with relatively high h2, in two out of three populations indicated that marker-assisted selection was superior to phenotypic selection. The effectiveness of MAS on enhancing stand establishment and eating quality will be discussed.

INHERITANCE AND ASSOCIATION OF SEEDS PER HEAD, YIELD PER HEAD, BLOTCH, AND FLOWER COLOR IN SAFFLOWER SPECIES

1980 ◽  
Vol 60 (3) ◽  
pp. 813-819 ◽  
Author(s):  
ASHOK KOTECHA
Keyword(s):  
Significant Positive Correlation ◽  
Gene Action ◽  
Flower Color ◽  
Carthamus Tinctorius ◽  
Broad Sense Heritability ◽  
Anthocyanin Pigmentation ◽  
Positive Correlation Coefficient ◽  
Multiple Factor ◽  
Mode Of Inheritance ◽  
Map Distance

Mode of inheritance and association of seeds per head, yield per head, blotch and flower color were studied in two crosses of safflower (Carthamus tinctorius L.). Data were taken on individual plants. Mather and Jinks’ three-parameter model indicated that gene action for seeds per head and yield per head showed additive and dominance gene action. The broad-sense heritability for seeds per head and yield per head ranged from 50.75 to 56.02%, and 50 to 57.14%, respectively. Maternal effects were absent for seeds per head and yield per head. In all F2, continuous distributions were observed, suggesting multiple factor inheritance for seeds per head and yield per head. Two loci may be involved in the inheritance of blotch (a new trait) and flower color. Flower color and blotch were inherited independently. Also, flower color and striped hull were inherited independently. A 18 ± 5.6 map distance was found between anthocyanin pigmentation and blotch. Seeds per head and yield per head were not associated with anthocyanin pigmentation, striped hull, flower color and blotch. A significant-positive correlation coefficient ranging from 0.86 to 0.88 was found between seeds per head and yield per head.

INHERITANCE AND LINKAGE OF A MAGENTA FLOWER GENE IN SOYBEANS

1977 ◽  
Vol 19 (4) ◽  
pp. 749-751 ◽  
Author(s):  
R. I. Buzzell ◽  
B. R. Buttery ◽  
R. L. Bernard
Keyword(s):  
Glycine Max ◽  
Linkage Group ◽  
Photosynthetic Rate ◽  
Flower Color ◽  
Mutant Gene ◽  
Common Gene ◽  
Glycine Max L ◽  
Bean Yield ◽  
The Common

Magenta flower color in soybeans (Glycine max (L.) Merr.) was found to be controlled by a mutant gene, wm, in the presence of W1 which is the common gene for purple flowers. These two genes are closely linked (2.2 ± 0.7% recombination) and are members of what is proposed as Linkage Group 8. The wm reduces flavonol content of flowers and of leaves, and is a deleterious mutant in terms of photosynthetic rate and bean yield.

Sign in / Sign up

Export Citation Format

Share Document