Identification of genes for resistance to a Digitaria isolate of Magnaporthe grisea in common wheat cultivars

Genome ◽  
2009 ◽  
Vol 52 (9) ◽  
pp. 801-809 ◽  
Author(s):  
N. T.T. Nga ◽  
V. T.B. Hau ◽  
Y. Tosa
Keyword(s):  
High Temperature ◽  
Common Wheat ◽  
Magnaporthe Grisea ◽  
Major Gene ◽  
Monosomic Analysis ◽  
Wheat Cultivars ◽  
Substitution Lines ◽  
Chromosome Substitution Lines ◽  
Blast Fungus ◽  
Specific Species

Common wheat cultivars are resistant to Magnaporthe grisea , a crabgrass ( Digitaria sanguinalis )-specific species of the blast fungus. To dissect the genetic basis of this “nonhost” type of resistance, we need an exceptional cultivar that is susceptible to M. grisea. A screening under various conditions revealed that Triticum aestivum ‘Chinese Spring’ (CS) was susceptible to M. grisea isolate Dig41 when incubated at high temperature (26 °C) after inoculation. By contrast, T. aestivum ‘P168’, ‘Shin-chunaga’ (Sch), ‘Norin 4’ (N4), ‘Norin 26’ (N26), ‘Norin 29’ (N29), ‘Red Egyptian’ (RE), and ‘Salmon’ (Slm) and Triticum compactum ‘No. 44’ (Cmp) were highly resistant even at the high temperature. When F2 seedlings derived from crosses between the resistant cultivars and CS were inoculated with Dig41, they segregated in a 3:1 ratio of resistant to susceptible, suggesting that the resistance of each cultivar is controlled by one major gene. Crosses of N4 with P168, Sch, N26, N29, and Cmp yielded no susceptible F2 seedlings, suggesting that these six cultivars share the same gene. Similarly, a cross between RE and Slm yielded no susceptible F2 seedlings, suggesting that these two cultivars share the same gene. On the other hand, crosses between the N4 group and the RE group produced resistant and susceptible seedlings in a 15:1 ratio, indicating that these two groups carry different genes inherited independently. The gene in N4 was located on chromosome 4A by a monosomic analysis and designated Rmg4, while the gene in RE was located on chromosome 6D using a series of chromosome substitution lines and designated Rmg5. These results suggest that the resistance of common wheat to M. grisea, an inappropriate species of the blast fungus, is under a simple genetic control.

The chromosomal locations in wheat of genes conferring differential response to the wild oat herbicide, difenzoquat

1987 ◽  
Vol 108 (3) ◽  
pp. 543-548 ◽  
Author(s):  
J. W. Snape ◽  
W. J. Angus ◽  
Beryl Parker ◽  
Debra Leckie
Keyword(s):  
Chinese Spring ◽  
Major Gene ◽  
Wheat Variety ◽  
Differential Response ◽  
Susceptible Variety ◽  
Monosomic Analysis ◽  
Wild Oat ◽  
Substitution Lines ◽  
Single Chromosome ◽  
Chromosome Substitution Lines

SummaryF2, monosomic analysis involving crosses between the monosomic series of a resistant wheat variety, Chinese Spring, and a susceptible variety, Sicco, has located a major gene locus, designated Dfql, on chromosome 2B of wheat which determines the differential response of these varieties to treatment with the wild oat herbicide, difenzoquat. The allele from Chinese Spring conferring resistance is dominant and studies of the responses of Chinese Spring single chromosome substitution lines and nullisomic–tetrasomic lines for chromosome 2B indicate that this allele actively promotes resistance to the herbicide. It is suggested that this gene may prevent inhibition of DNA synthesis in the apical meristem, which is the site of action of the herbicide (Pallett & Caseley, 1980).Other chromosomes were also implicated as carrying ‘modifier genes’ which affect the ratio of resistant: susceptible plants in F2 monosomic families, namely 1D, 2D, 3A, 3B, 5B and 5D. These chromosomes may affect the retention and translocation of the herbicide to the target site and hence the threshold of response.The simple inheritance of difenzoquat resistance indicates that it should be easy by conventional breeding techniques to transfer the resistance into susceptible varieties.

PERFORMANCE OF HYBRID POPULATIONS DERIVED FROM CROSSES OF SUBSTITUTION LINES OF COMMON WHEAT

1971 ◽  
Vol 13 (4) ◽  
pp. 816-821
Author(s):  
Sadeque U. Ahmed
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Kernel Weight ◽  
Kernel Number ◽  
Substitution Lines ◽  
Breeding Programs ◽  
Chromosome Substitution Lines ◽  
Quantitative Characters

The recipient variety 'Chinese Spring', chromosome substitution lines 1B of Timstein' and 4A of 'Thatcher', and donor varieties Timstein and Thatcher were studied with respect to six quantitative characters, viz. earliness, plant height, tiller number per plant, kernel number per spike, 1000-kernel weight and total grain yield per plant. Heterosis was observed for all characters; however, the degree and direction of heterosis varied for different characters and for different hybrid populations. Evidence for significant improvement in kernel weight and total grain yield per plant combined with early heading and short plant height were obtained. Evidence was obtained indicating that substitution lines may be effective breeding materials in common wheat (Triticum aestivum L.) breeding programs.

scholarly journals Identification and Molecular Mapping of a Wheat Gene for Resistance to an Unadapted Isolate of Colletotrichum cereale

2013 ◽  
Vol 103 (6) ◽  
pp. 575-582 ◽  
Author(s):  
Yoshihiro Inoue ◽  
Ryota Mori ◽  
Yujiro Takahashi ◽  
So Kiguchi ◽  
Takashi Enomoto ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Major Gene ◽  
Hypersensitive Reaction ◽  
Substitution Lines ◽  
Resistant Cultivars ◽  
Chromosome Substitution Lines ◽  
Colletotrichum Cereale ◽  
Wheat Gene ◽  
Genetic Mechanisms ◽  
Chromosome 5A

To elucidate genetic mechanisms of host species specificity between graminicolous anthracnose fungi and gramineous plants, infection assays were performed with a Sorghum isolate (Colletotrichum sublineolum), a Digitaria isolate (C. hanaui), a Polypogon isolate (C. cereale), and an Avena isolate (C. cereale). They were specifically virulent on the plants from which they were isolated. When 72 wheat lines were inoculated with an unadapted isolate from Asia Minor bluegrass (Cgp29), however, some exceptional cultivars were recognized. Although most cultivars were resistant to Cgp29, ‘Hope’ was susceptible. In F2 populations derived from crosses between three resistant cultivars—‘Norin 4’ (N4), ‘Chinese Spring’ (CS), and ‘Shin-chunaga’ (Sch)—and the susceptible Hope, resistant and susceptible seedlings segregated in a 3:1 ratio, suggesting that a major gene is involved in the resistance of each cultivar to Cgp29. In F2 populations derived from crosses between the three resistant cultivars, all seedlings were resistant, suggesting that these three cultivars carry the same gene. This resistance gene was designated as “resistance to Colletotrichum cereale 1” (Rcc1). Analysis with the CS–Hope chromosome substitution lines and molecular mapping revealed that Rcc1 was located on the long arm of chromosome 5A. Cytologically, Rcc1 was mainly associated with hypersensitive reaction. These results suggest that major genes similar to those controlling cultivar specificity are involved in the resistance of wheat against the unadapted isolate of C. cereale.

Chromosomes in 'Cadet' and 'Rescue' wheats carrying loci for cold hardiness and vernalization response

1986 ◽  
Vol 28 (6) ◽  
pp. 991-997 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Triticum Aestivum ◽  
Spring Wheat ◽  
Cold Hardiness ◽  
Rank Order ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Chromosome Substitution ◽  
Vernalization Response ◽  
Substitution Lines ◽  
Chromosome Substitution Lines

'Rescue', 'Cadet', and the 42 reciprocal chromosome substitution lines derived from these two spring wheat cultivars were tested for vernalization response and cold hardiness. Cold hardiness was tested after hardening under a 16-h day for 8 weeks with 6 °C day and 4 °C night temperatures or in the dark for 7 weeks at 0.8 °C followed by 8 weeks at −5 °C. Chromosomes 5A, 5B, 7B, and possibly 2A carried loci for vernalization response. Chromosomes 2A, 5A, and 5B carried loci affecting cold hardiness measured after 8 weeks in the light at 6 °C during the day and 4 °C at night, whereas chromosomes 6A, 3B, 5B, and 5D were involved in cold hardiness after hardening in the dark at 0.8 °C followed by −5 °C. The results suggest that the rank order of cultivars for cold hardiness depends on the hardening technique used since the two different techniques tested had different genetic and presumably somewhat different biochemical bases.Key words: Triticum aestivum L., cold hardiness, vernalization.

scholarly journals High-Temperature and Drought-Resilience Traits among Interspecific Chromosome Substitution Lines for Genetic Improvement of Upland Cotton

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1747
Author(s):  
Kambham Raja Reddy ◽  
Raju Bheemanahalli ◽  
Sukumar Saha ◽  
Kulvir Singh ◽  
Suresh B. Lokhande ◽  
...  
Keyword(s):  
High Temperature ◽  
Stomatal Conductance ◽  
Upland Cotton ◽  
Pollen Germination ◽  
Chromosome Substitution ◽  
Multiple Traits ◽  
Substitution Lines ◽  
Phenotypic Data ◽  
Chromosome Substitution Lines ◽  
Tolerance Indices

Upland cotton (Gossypium hirsutum L.) growth and development during the pre-and post-flowering stages are susceptible to high temperature and drought. We report the field-based characterization of multiple morpho-physiological and reproductive stress resilience traits in 11 interspecific chromosome substitution (CS) lines isogenic to each other and the inbred G. hirsutum line TM-1. Significant genetic variability was detected (p < 0.001) in multiple traits in CS lines carrying chromosomes and chromosome segments from CS-B (G. barbadense) and CS-T (G. tomentosum). Line CS-T15sh had a positive effect on photosynthesis (13%), stomatal conductance (33%), and transpiration (24%), and a canopy 6.8 °C cooler than TM-1. The average pollen germination was approximately 8% greater among the CS-B than CS-T lines. Based on the stress response index, three CS lines are identified as heat- and drought-tolerant (CS-T07, CS-B15sh, and CS-B18). The three lines demonstrated enhanced photosynthesis (14%), stomatal conductance (29%), transpiration (13%), and pollen germination (23.6%) compared to TM-1 under field conditions, i.e., traits that would expectedly enhance performance in stressful environments. The generated phenotypic data and stress-tolerance indices on novel CS lines, along with phenotypic methods, would help in developing new cultivars with improved resilience to the effects of global warming.

The location and effects of genes modifying the response of wheat to the herbicide difenzoquat

1992 ◽  
Vol 118 (1) ◽  
pp. 9-15
Author(s):  
D. Leckie ◽  
J. W. Snape
Keyword(s):  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Susceptible Variety ◽  
Modifier Genes ◽  
Monosomic Analysis ◽  
Chromosome Substitution ◽  
Substitution Lines ◽  
Single Chromosome ◽  
Chromosome Substitution Lines ◽  
Intermediate Response

SUMMARYSingle chromosome substitution lines of hexaploid wheat were developed using a variety resistant to difenzoquat, Chinese Spring, as donor and a susceptible variety, Sicco, as recipient, and were used to identify chromosomes carrying genes which modify the responses of these varieties. It was found that chromosomes 3B and 5D from Chinese Spring might act to reduce the amount of damage caused by the herbicide in the presence of the allele for susceptibility at the Dfql locus. The intermediate response to the herbicide, which is shown by some commerical varieties, was also investigated using a backcross reciprocal monosomic analysis. In these varieties, the allele at the Dfql locus determining the reaction to the herbicide was shown to be similar to that of the susceptible variety Sicco. It is, therefore, probable that the responses of intermediate varieties are due to the effects of modifier genes increasing resistance.

MONOSOMIC ANALYSIS OF GENES CONDITIONING STEM RUST RESISTANCE IN TWO COMMON WHEAT CULTIVARS

1974 ◽  
Vol 16 (2) ◽  
pp. 281-284
Author(s):  
A. K. Sanghi ◽  
E. P. Baker
Keyword(s):  
Common Wheat ◽  
Stem Rust ◽  
Somatic Hybrid ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Puccinia Graminis ◽  
Monosomic Analysis ◽  
Wheat Cultivars ◽  
Wheat Stem Rust ◽  
Chromosome 3B

The single genes in the cultivars Morocco and Yalta conditioning resistance to stem rust culture 103-H-2, a somatic hybrid between wheat stem rust (Puccinia graminis tritici) and rye stem rust (P. graminis secalis) which possesses unusual genes for avirulence on wheat, were each located by monosomic analysis on chromosome 3B. They were estimated to be approximately 9 map units apart.

Characteristics of Photosynthesis in Different Wheat Cultivars under High Light Intensity and High Temperature Stresses

2009 ◽  
Vol 34 (12) ◽  
pp. 2196-2201 ◽  
Author(s):  
Xue-Li QI ◽  
Lin HU ◽  
Hai-Bin DONG ◽  
Lei ZHANG ◽  
Gen-Song WANG ◽  
...  
Keyword(s):  
High Temperature ◽  
Light Intensity ◽  
High Light Intensity ◽  
High Light ◽  
Wheat Cultivars ◽  
Temperature Stresses

scholarly journals Effects of Interspecific Chromosome Substitution in Upland Cotton on Cottonseed Macronutrients

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1158
Author(s):  
Nacer Bellaloui ◽  
Sukumar Saha ◽  
Jennifer L. Tonos ◽  
Jodi A. Scheffler ◽  
Johnie N. Jenkins ◽  
...  
Keyword(s):  
South Carolina ◽  
Upland Cotton ◽  
Field Experiments ◽  
Seed Quality ◽  
Recurrent Parent ◽  
Chromosome Substitution ◽  
Substitution Lines ◽  
Chromosome Substitution Lines ◽  
Macronutrient Content ◽  
Chromosome Arm

Nutrients, including macronutrients such as Ca, P, K, and Mg, are essential for crop production and seed quality, and for human and animal nutrition and health. Macronutrient deficiencies in soil lead to poor crop nutritional qualities and a low level of macronutrients in cottonseed meal-based products, leading to malnutrition. Therefore, the discovery of novel germplasm with a high level of macronutrients or significant variability in the macronutrient content of crop seeds is critical. To our knowledge, there is no information available on the effects of chromosome or chromosome arm substitution on cottonseed macronutrient content. The objective of this study was to evaluate the effects of chromosome or chromosome arm substitution on the variability and content of the cottonseed macronutrients Ca, K, Mg, N, P, and S in chromosome substitution lines (CS). Nine chromosome substitution lines were grown in two-field experiments at two locations in 2013 in South Carolina, USA, and in 2014 in Mississippi, USA. The controls used were TM-1, the recurrent parent of the CS line, and the cultivar AM UA48. The results showed major variability in macronutrients among CS lines and between CS lines and controls. For example, in South Carolina, the mean values showed that five CS lines (CS-T02, CS-T04, CS-T08sh, CS-B02, and CS-B04) had higher Ca level in seed than controls. Ca levels in these CS lines varied from 1.88 to 2.63 g kg−1 compared with 1.81 and 1.72 g kg−1 for TM-1 and AMUA48, respectively, with CS-T04 having the highest Ca concentration. CS-M08sh exhibited the highest K concentration (14.50 g kg−1), an increase of 29% and 49% over TM-1 and AM UA48, respectively. Other CS lines had higher Mg, P, and S than the controls. A similar trend was found at the MS location. This research demonstrated that chromosome substitution resulted in higher seed macronutrients in some CS lines, and these CS lines with a higher content of macronutrients can be used as a genetic tool towards the identification of desired seed nutrition traits. Also, the CS lines with higher desired macronutrients can be used as parents to breed for improved nutritional quality in Upland cotton, Gossypium hirsutum L., through improvement by the interspecific introgression of desired seed nutrient traits such as Ca, K, P, S, and N. The positive and significant (p ≤ 0.0001) correlation of P with Ca, P with Mg, S with P, and S with N will aid in understanding the relationships between nutrients to improve the fertilizer management program and maintain higher cottonseed nutrient content.

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