Isolation and characterization of the rye Waxy gene

Genome ◽  
2009 ◽  
Vol 52 (7) ◽  
pp. 658-664 ◽  
Author(s):  
Jie Xu ◽  
Michele Frick ◽  
André Laroche ◽  
Zhong-Fu Ni ◽  
Bao-Yun Li ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Evolutionary Relationship ◽  
Waxy Gene ◽  
Coding Region ◽  
Gene Encoding ◽  
Sequence Comparisons ◽  
Cdna Sequences ◽  
Isolation And Characterization ◽  
Complete Genomic

Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns. The rye Waxy gene is GC-rich, with a higher GC frequency in the coding region, especially in the third position of the codons. Exon regions of the rye Waxy gene are more conserved than intron regions when compared with the homologous sequences of other cereals. The mature rye GBSSI proteins share more than 95% sequence identity with their homologs in wheat and barley. A phylogenetic tree based on sequence comparisons of available plant GBSSI proteins shows the evolutionary relationship among Waxy genes from rye and other plant genomes. The identification of the rye Waxy gene will enable the manipulation of starch metabolism in rye and triticale.

scholarly journals Complete Genomic Sequence of the Virulent Salmonella Bacteriophage SP6

2004 ◽  
Vol 186 (7) ◽  
pp. 1933-1944 ◽  
Author(s):  
Aleisha T. Dobbins ◽  
Matthew George ◽  
Daryl A. Basham ◽  
Michael E. Ford ◽  
Jennifer M. Houtz ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Genomic Sequence ◽  
Bioinformatic Analysis ◽  
Complete Genomic Sequence ◽  
Coding Region ◽  
Gene Encoding ◽  
Serovar Typhimurium ◽  
The Right ◽  
Direct Terminal Repeat ◽  
Complete Genomic

ABSTRACT We report the complete genome sequence of enterobacteriophage SP6, which infects Salmonella enterica serovar Typhimurium. The genome contains 43,769 bp, including a 174-bp direct terminal repeat. The gene content and organization clearly place SP6 in the coliphage T7 group of phages, but there is ∼5 kb at the right end of the genome that is not present in other members of the group, and the homologues of T7 genes 1.3 through 3 appear to have undergone an unusual reorganization. Sequence analysis identified 10 putative promoters for the SP6-encoded RNA polymerase and seven putative rho-independent terminators. The terminator following the gene encoding the major capsid subunit has a termination efficiency of about 50% with the SP6-encoded RNA polymerase. Phylogenetic analysis of phages related to SP6 provided clear evidence for horizontal exchange of sequences in the ancestry of these phages and clearly demarcated exchange boundaries; one of the recombination joints lies within the coding region for a phage exonuclease. Bioinformatic analysis of the SP6 sequence strongly suggested that DNA replication occurs in large part through a bidirectional mechanism, possibly with circular intermediates.

Isolation and characterization of a bovine gene encoding phenol sulfotransferase

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Travis Henry ◽  
Brian Kliewer ◽  
Robert Palmatier ◽  
Joseph S. Ulphani ◽  
Joe D. Beckmann
Keyword(s):  
Gene Encoding ◽  
Bovine Gene ◽  
Isolation And Characterization ◽  
Phenol Sulfotransferase

Characterization of Salt Overly Sensitive 1 (SOS1) gene homoeologs in quinoa (Chenopodium quinoa Willd.)

Genome ◽  
2009 ◽  
Vol 52 (7) ◽  
pp. 647-657 ◽  
Author(s):  
P. J. Maughan ◽  
T. B. Turner ◽  
C. E. Coleman ◽  
D. B. Elzinga ◽  
E. N. Jellen ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Dna Sequences ◽  
Genomic Sequence ◽  
Chenopodium Quinoa ◽  
Fish Analysis ◽  
Cdna Sequences ◽  
Grain Crop ◽  
High Level ◽  
Salt Overly Sensitive

Salt tolerance is an agronomically important trait that affects plant species around the globe. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth of plants in saline environments. Quinoa (Chenopodium quinoa Willd.) is a halophytic, allotetraploid grain crop of the family Amaranthaceae with impressive nutritional content and an increasing worldwide market. Many quinoa varieties have considerable salt tolerance, and research suggests quinoa may utilize novel mechanisms to confer salt tolerance. Here we report the cloning and characterization of two homoeologous SOS1 loci (cqSOS1A and cqSOS1B) from C. quinoa, including full-length cDNA sequences, genomic sequences, relative expression levels, fluorescent in situ hybridization (FISH) analysis, and a phylogenetic analysis of SOS1 genes from 13 plant taxa. The cqSOS1A and cqSOS1B genes each span 23 exons spread over 3477 bp and 3486 bp of coding sequence, respectively. These sequences share a high level of similarity with SOS1 homologs of other species and contain two conserved domains, a Nhap cation-antiporter domain and a cyclic-nucleotide binding domain. Genomic sequence analysis of two BAC clones (98 357 bp and 132 770 bp) containing the homoeologous SOS1 genes suggests possible conservation of synteny across the C. quinoa sub-genomes. This report represents the first molecular characterization of salt-tolerance genes in a halophytic species in the Amaranthaceae as well as the first comparative analysis of coding and non-coding DNA sequences of the two homoeologous genomes of C. quinoa.

Isolation and characterization of the gene encoding the muscle-specific isozyme of human phosphoglycerate mutase

Gene ◽  
1990 ◽  
Vol 91 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Judit Castella-Escola ◽  
David M. Ojcius ◽  
Philippe LeBoulch ◽  
Virginie Joulin ◽  
Yves Blouquit ◽  
...  
Keyword(s):  
Phosphoglycerate Mutase ◽  
Gene Encoding ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

Viruses ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 108
Author(s):  
Wang Liu ◽  
Xiaowei Zheng ◽  
Xin Dai ◽  
Zhenfeng Zhang ◽  
Wenyan Zhang ◽  
...  
Keyword(s):  
Marine Sediments ◽  
Hydrothermal Vent ◽  
Okinawa Trough ◽  
Marine Ecosystem ◽  
Genome Replication ◽  
Gene Encoding ◽  
Significant Similarity ◽  
Host Chromosome ◽  
Isolation And Characterization

Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3′-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (≥70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.

Isolation and characterization of theATF2 gene encoding alcohol acetyltransferase II in the bottom fermenting yeastSaccharomyces pastorianus

Yeast ◽  
1999 ◽  
Vol 15 (5) ◽  
pp. 409-417 ◽  
Author(s):  
Hiroyuki Yoshimoto ◽  
Daisuke Fujiwara ◽  
Takayuki Momma ◽  
Keiko Tanaka ◽  
Hidetaka Sone ◽  
...  
Keyword(s):  
Gene Encoding ◽  
Alcohol Acetyltransferase ◽  
Isolation And Characterization
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