A new gene for resistance to Dysaphis pyri in pear and identification of flanking microsatellite markers

Genome ◽  
2008 ◽  
Vol 51 (12) ◽  
pp. 1026-1031 ◽  
Author(s):  
Kate M. Evans ◽  
Ceri L. Govan ◽  
Felicidad Fernández-Fernández

Dysaphis pyri is an important aphid pest of European pear ( Pyrus communis ) cultivars, none of which are currently reported to be resistant. In this study, we produced a progeny of the European pear Comice crossed with an accession of snow pear (Pyrus nivalis) that segregated for resistance to D. pyri in a Mendelian fashion, indicating the presence of a major gene, Dp-1. Following screening of the parents and seedlings with microsatellite markers, cosegregation analysis indicated that Dp-1 is flanked by NH006b and NH014a on linkage group 17, 2.3 and 3.6 cM away, respectively. Evidence is also presented for the duplication of linkage groups 9 and 17, which is a consequence of the allopolyploid origin of pear.

2013 ◽  
Vol 157 ◽  
pp. 74-83 ◽  
Author(s):  
Fuad Gasi ◽  
Mirsad Kurtovic ◽  
Belma Kalamujic ◽  
Naris Pojskic ◽  
Jasmin Grahic ◽  
...  

Genetics ◽  
1993 ◽  
Vol 133 (4) ◽  
pp. 865-874
Author(s):  
J A Holmes ◽  
D E Johnson ◽  
S K Dutcher

Abstract Linkage group XIX (or the UNI linkage group) of Chlamydomonas reinhardtii has been reported to show a circular meiotic recombination map. A circular map predicts the existence of strong chiasma and chromatid interference, which would lead to an excess number of two-strand double crossovers during meiosis. We have tested this prediction in multipoint crosses. Our results are consistent with a linear linkage group that shows positive chiasma interference and no chromatid interference. Chiasma interference occurs both within arms and across the centromere. Of the original loci that contributed to the circular map, we find that two map to other linkage groups and a third cannot be retested because the mutant strain that defined it has been lost. A second reported unusual property for linkage group XIX was the increase in meiotic recombination with increases in temperature during a period that precedes the onset of meiosis. Although we observed changes in recombination frequencies in some intervals on linkage group XIX in crosses to CC-1952, and in strains heterozygous for the mutation ger1 at 16 degrees, we also show that our strains do not exhibit the previously observed patterns of temperature-sensitive recombination for two different pairs of loci on linkage group XIX. We conclude that linkage group XIX has a linear genetic map that is not significantly different from other Chlamydomonas linkage groups.


Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 544-551 ◽  
Author(s):  
Yonghe Bai ◽  
T. E. Michaels ◽  
K. P. Pauls

Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.Key words: Phaseolus vulgaris L., common bacterial blight (CBB), polymerase chain reaction (PCR), RAPD markers, linkage groups.


1984 ◽  
Vol 26 (6) ◽  
pp. 762-764 ◽  
Author(s):  
Peter S. Dawson

Reindeer (Rd) is a dominant mutation affecting antenna morphology in the tenebrionid flour beetle, Tribolium castaneum. In contrast with most dominant mutants previously described for this species, homozygotes are fully viable, thus making Rd very useful for genetic studies. Rd is tentatively assigned to either linkage group IX or X. Abbreviated appendages (aa), formerly placed in linkage group X, is reassigned to linkage group V on the basis of demonstrated linkage to jet (j).Key words: Tribolium, mutation Rd, linkage, antenna morphology.


Helia ◽  
2000 ◽  
Vol 23 (33) ◽  
pp. 65-76
Author(s):  
V.V. Kirichenko ◽  
V.N. Popov

SUMMARY The genetics of anodal esterase (Est), cathodal esterase (cEst), cathodal acid phosphatase (cAcp) and malate dehydrogenase (Mdh) has been studied in mature seeds and leaves (genetics of cAcp and Mdh has not been studied in leaves) of sunflower (Helianthus annuus L.). A total of ten loci (four loci of anodal esterase, two loci of cathodal esterase, three loci of malate dehydrogenase and one locus of cathodal acid phosphatase) have been identified and described. Five esterase loci (Est1, Est2, Est3, Est4, cEst5), three malate dehydrogenase loci and one locus of cathodal acid phosphatase are expressed in seeds. Three esterase loci (Est2, cEst5 and cEst6) are expressed in leaves. The analysis of linkage between these loci has been made. Two linkage groups have been found. The sequence of the loci in the first linkage group was Mdh2-Est1- Est2-Est3-cEst5. In the second linkage group it was Est4-cAcp1. Linkages have been analyzed between three isoenzymatic loci expressed in leaves and between two loci controlling morphological traits (branched stem and male fertility restoration). The linkage between morphological traits and isoenzymatic loci has not been revealed. It has been revealed in Br-Rf pair.


Genetics ◽  
1981 ◽  
Vol 99 (3-4) ◽  
pp. 415-428
Author(s):  
Raja E Rosenbluth ◽  
David L Baillie

ABSTRACT The Caenorhabditis elegans mutation e873, which results in a recessive uncoordinated phenotype (formerly named Unc-72) and which had been isolated after 32P t reatment (BRENNER1 974), has now been found to act as a crossover suppressor and to be associated with a translocation between linkage groups (LG's) IIIand V. The translocation has been named, eTl(ZI1; V); eT1acts as a dominant crossover suppressor for both the right half of LGIIIand the left half of LGV,providing a balancer for a total of 39 map units. The uncoordinated e873phenotype has been shown to be a consequence of Eminactive unr- 36111gene. It was possible to demonstrate that, in translocation heterozygotes, eT1chromosomes marked with either sma-3or dpy-11segregate from normal LGIII,while those marked with bli-5, sm-2or unc-42segregate from normal LGV.Since bli-5and sma-2are normally on LGIII,and dpy-11is normally on LGV,it is concluded that: (a) eT1is a reciprocal translocation; (b) there is a breakpoint between sma-3and sma-2in LGIII(the region containing unc- 36)and one between dpy-11and unc-42in LGV;(c) thera is no dominant centromere between sma-2and bli-5on LGIII,since in eT1these genes are not linked to a LGIIIcentromere. Similarly, it is highly unlikely that there is a centromere to the left of dpy-11on LGV.The new gene order in eT1was determined by measuring recombination rates between markers in eT1homozygotes. It is concluded that the new order is: dpy-1 sma-3 (break) dpy-11 unc-60,and bli-5 sma-2 (break) unc-42 unc-51.——Thisis the first analysis of a C. eleganstranslocation with respect to reciprocity, breakpoints and new gene order.


1995 ◽  
Vol 68 (1-2) ◽  
pp. 107-111 ◽  
Author(s):  
Y. Du ◽  
E.F. Remmers ◽  
H. Zha ◽  
E.A. Goldmuntz ◽  
P. Mathern ◽  
...  

2021 ◽  
Author(s):  
Julie Graham ◽  
Kay Smith ◽  
Katrin MacKenzie ◽  
Linda Milne ◽  
Nikki Jennings ◽  
...  

Abstract Background The changing climate is altering timing of key fruit ripening processes and increasing the occurrence of fruit defects. This work aimed to expand our knowledge of the genetic control of the ripening process in raspberry by examining a biennial x primocane F1 population to determine if the progeny exhibited both primocane and biennial flowering modes, which if any was dominant, and to identify QTL and genome locations associated with fruit development to understand how developmental control in this population differs from a biennial x biennial F1 population previously studied. Results The progeny from this biennial x primocane population exhibited primocane fruiting completing their lifecycle in a single season and also fruiting on second-year wood not removed in season one. QTL associated with rate of fruit development were identified on both primocane and fruiting canes with both parents impacting. Conclusions Novel QTL associated with the developmental process of primocane fruiting were identified. These in the main, differed from developmental QTL for similar developmental stages on fruiting canes (second year canes) with only one significant overlap on linkage group 6. In general, the process of development on fruiting canes overall differed from that in a biennial x biennial population, with the differences being greatest on linkage groups 3 and 6 suggesting control of development differs in the different fruiting types. Further understanding will be achieved by examining genome regions linked to QTL to allow breeding to meet climate requirements for yield stability.


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