Loci affecting flowering time in oat under short-day conditions

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1528-1538 ◽  
Author(s):  
Ana B. Locatelli ◽  
Luiz C. Federizzi ◽  
Sandra C. K. Milach ◽  
Charlene P. Wight ◽  
Stephen J. Molnar ◽  
...  
Keyword(s):  
Flowering Time ◽  
Comparative Mapping ◽  
Bulked Segregant Analysis ◽  
Recombinant Inbred Lines ◽  
Rice Genome ◽  
Reference Population ◽  
Day Length ◽  
Short Day ◽  
Growing Seasons ◽  
Trait Locus

Flowering time (or days to heading) is an important characteristic in crop plants that affects adaptation to cropping cycles and growing seasons. The objectives of this study were to identify molecular markers associated with flowering time in 3 oat populations developed from Brazilian oat varieties, and to compare their map locations with those of other loci that might influence flowering time. Flowering time was studied in recombinant inbred lines from 3 hexaploid oat populations: UFRGS 8 × Pc68/5*Starter; UFRGS 881971 × Pc68/5*Starter; and UFRGS 8 × UFRGS 930605. Bulked segregant analysis, using amplified fragment length polymorphism, was followed by selective mapping in each population and in a reference population, ‘Kanota’ × ‘Ogle’ (K×O). One quantitative trait locus (QTL) with major effects on flowering time was identified in each cross. Comparative mapping showed that a major QTL, with earliness alleles originating from UFRGS 8 and UFRGS 881971, is in a region with close homology to K×O linkage group 17 and to a locus that reportedly confers day-length insensitivity in oat (Di1). This is the first report to identify the map location of the Di1 locus, and putatively confirm the presence of Di1 alleles in new germplasm. Further comparative mapping and the alignment of mapped oat markers with the sequenced rice genome suggest that this QTL and (or) Di1 is orthologous to the Hd1 locus in rice and the CONSTANS gene in Arabidopsis and other species. A different QTL with major effects segregated in the UFRGS 8 × UFRGS 930605 cross, where the early-flowering allele for Di1 was probably fixed. Two additional QTLs with smaller effects were identified in the UFRGS 8 × Pc68/5*Starter population. These results suggest that the Brazilian oat line UFRGS 8 contains an optimal set of alleles conditioning earliness under the short-day conditions of the Brazilian winter growing season, and that molecular selection could be used to introgress these alleles into other breeding material.

scholarly journals Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0240390
Author(s):  
Hongxu Dong ◽  
Lindsay V. Clark ◽  
Xiaoli Jin ◽  
Kossonou Anzoua ◽  
Larisa Bagmet ◽  
...  
Keyword(s):  
Flowering Time ◽  
High Latitude ◽  
Floral Induction ◽  
Day Treatment ◽  
Day Length ◽  
Close Relative ◽  
Short Day ◽  
Culm Length ◽  
Low Latitudes ◽  
Short Days

Miscanthus is a close relative of Saccharum and a potentially valuable genetic resource for improving sugarcane. Differences in flowering time within and between Miscanthus and Saccharum hinders intra- and interspecific hybridizations. A series of greenhouse experiments were conducted over three years to determine how to synchronize flowering time of Saccharum and Miscanthus genotypes. We found that day length was an important factor influencing when Miscanthus and Saccharum flowered. Sugarcane could be induced to flower in a central Illinois greenhouse using supplemental lighting to reduce the rate at which days shortened during the autumn and winter to 1 min d-1, which allowed us to synchronize the flowering of some sugarcane genotypes with Miscanthus genotypes primarily from low latitudes. In a complementary growth chamber experiment, we evaluated 33 Miscanthus genotypes, including 28 M. sinensis, 2 M. floridulus, and 3 M. ×giganteus collected from 20.9° S to 44.9° N for response to three day lengths (10 h, 12.5 h, and 15 h). High latitude-adapted M. sinensis flowered mainly under 15 h days, but unexpectedly, short days resulted in short, stocky plants that did not flower; in some cases, flag leaves developed under short days but heading did not occur. In contrast, for M. sinensis and M. floridulus from low latitudes, shorter day lengths typically resulted in earlier flowering, and for some low latitude genotypes, 15 h days resulted in no flowering. However, the highest ratio of reproductive shoots to total number of culms was typically observed for 12.5 h or 15 h days. Latitude of origin was significantly associated with culm length, and the shorter the days, the stronger the relationship. Nearly all entries achieved maximal culm length under the 15 h treatment, but the nearer to the equator an accession originated, the less of a difference in culm length between the short-day treatments and the 15 h day treatment. Under short days, short culms for high-latitude accessions was achieved by different physiological mechanisms for M. sinensis genetic groups from the mainland in comparison to those from Japan; for mainland accessions, the mechanism was reduced internode length, whereas for Japanese accessions the phyllochron under short days was greater than under long days. Thus, for M. sinensis, short days typically hastened floral induction, consistent with the expectations for a facultative short-day plant. However, for high latitude accessions of M. sinensis, days less than 12.5 h also signaled that plants should prepare for winter by producing many short culms with limited elongation and development; moreover, this response was also epistatic to flowering. Thus, to flower M. sinensis that originates from high latitudes synchronously with sugarcane, the former needs day lengths >12.5 h (perhaps as high as 15 h), whereas that the latter needs day lengths <12.5 h.

scholarly journals Genotyping by sequencing of 393 Sorghum bicolor BTx623 × IS3620C recombinant inbred lines improves sensitivity and resolution of QTL detection

2018 ◽  
Author(s):  
WenQian Kong ◽  
Changsoo Kim ◽  
Dong Zhang ◽  
Hui Guo ◽  
Xu Tan ◽  
...  
Keyword(s):  
Flowering Time ◽  
Plant Height ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Genotyping By Sequencing ◽  
Snp Markers ◽  
Ril Population ◽  
Trait Locus ◽  
Number Of Individuals ◽  
Syntenic Regions

AbstractWe describe a genetic map with a total of 381 bins of 616 genotyping by sequencing (GBS)-based SNP markers in a F6-F8 recombinant inbred line (RIL) population of 393 individuals derived from crossing S. bicolor BTx623 to S. bicolor IS3620C, a guinea line substantially diverged from BTx623. Five segregation distorted regions were found with four showing enrichment for S. bicolor alleles, suggesting possible selection during formation of this RIL population. A quantitative trait locus (QTL) study with this number of individuals, tripled relative to prior studies of this cross, provided resources, validated previous findings, and demonstrated improved power to detect plant height and flowering time related QTLs relative to other published studies. An unexpected low correlation between flowering time and plant height permitted us to separate QTLs for each trait and provide evidence against pleiotropy. Ten non-random syntenic regions conferring QTLs for the same trait suggest that those QTLs may represent alleles at genes functioning in the same manner since the 96 million year ago genome duplication that created these syntenic relationships, while syntenic regions conferring QTLs for different trait may suggest sub-functionalization after duplication. Collectively, this study provides resources for marker-assisted breeding, as well as a framework for fine mapping and subsequent cloning of major genes for important traits such as plant height and flowering time in sorghum.

scholarly journals Kadife (Tagetes erecta) Bitkisinde Gün Uzunluğunun Büyüme ve Çiçeklenme Üzerine Etkisi

2017 ◽  
Vol 5 (10) ◽  
pp. 1189 ◽  
Author(s):  
Nezihe Köksal ◽  
Sara Yasemin ◽  
Aslıhan Özkaya
Keyword(s):  
Flowering Time ◽  
Early Flowering ◽  
Day Length ◽  
Flowering Plant ◽  
Plant Canopy ◽  
Flower Bud ◽  
Bud Formation ◽  
Short Day ◽  
Long Day ◽  
Flower Bud Formation

Photoperiod is one of the environmental signals that controls of the flowering time on bedding plants. Marigold is a bedding plant which includes obligate or facultative short day and day neutral cultivars. Flowering time of these plants, even day neutral cultivars, delay in extreme hot and long day condition in summer. In this study, the effects of photoperiodic conditions (short day and long day) on flowering and growth of two different day neutral marigold cultivars (Discovery Orange and Discovery Yellow) were investigated. Natural day length (14 hours) was considered as long day condition. Short day condition (8 hours) was conducted artificially by darkening treatment. Therefore, duration to first flower bud formation, duration to first flowering, plant canopy height, plant canopy width, lateral branch number, flower number, main peduncle length, main peduncle thickness, root collar thickness, stem thickness, dry weights of plants (root, shoot, total plant) were evaluated. At the end of the experiment, it was determined that short day conditions reduce duration to first flower bud formation and duration to first flowering. The artificial short day conditions resulted as 13 days early flowering in 'Discovery Orange' and 5 days early flowering in 'Discovery Yellow' cultivar.

Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 856-864 ◽  
Author(s):  
Tomas Axelsson ◽  
Oksana Shavorskaya ◽  
Ulf Lagercrantz
Keyword(s):  
Arabidopsis Thaliana ◽  
Quantitative Trait Locus ◽  
Flowering Time ◽  
Comparative Mapping ◽  
Genome Duplication ◽  
Chromosomal Region ◽  
Diploid Species ◽  
Brassica Species ◽  
Ancestral Gene ◽  
Trait Locus

Quantitative trait locus (QTL) analysis was used to study the evolution of genes controlling the timing of flowering in four Brassica genomes that are all extensively replicated. Comparative mapping showed that a chromosomal region from the top of Arabidopsis thaliana chromosome 5 corresponded to three homoeologous copies in each of the diploid species Brassica nigra, B. oleracea, and B. rapa and six copies in the amphidiploid B. juncea. QTLs were detected in two of the three replicated segments in each diploid genome and in three of the six replicated segments in B. juncea. These results indicate that, for the studied trait, multiple QTLs resulting from genome duplication is the rule rather than the exception. Brassica homologues to two candidate genes (CO and FLC) identified from the corresponding A. thaliana region were mapped. CO homologues mapped close to the QTL peaks in eight of nine QTLs, while FLC homologues mapped farther away in those cases where the mapping resolution allowed a comparison. Thus, our data are consistent with the hypothesis that all the major QTLs we detected in the different species of Brassica could be the result of duplicated copies of the same ancestral gene, possibly the ancestor of CO.Key words: Brassica, Arabidopsis, comparative mapping, genome duplication, flowering time.

scholarly journals Effects of Short Day Conditioning, Chilling and GA3 Treatments to Yield and Fruit Quality in Strawberry Plug Transplants Aiming Early Fruit Production

2013 ◽  
Vol 41 (1) ◽  
pp. 263
Author(s):  
Emine ÖZDEMİR ◽  
Nurettin KAŞKA ◽  
Kazim GÜNDÜZ ◽  
Sedat SERÇE
Keyword(s):  
Fruit Quality ◽  
Total Yield ◽  
Titratable Acidity ◽  
Fruit Production ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Day Length ◽  
Fruit Characteristics ◽  
Short Day ◽  
Growing Seasons

In this study, we tested the effects of short day (SD), chilling (Ch) and gibberalic acid (GA3) treatments on the yield and fruit characteristics of ‘Camarosa’ and ‘Sweet Charlie’ plug plants under a freeze-protected greenhouse in Mediterranean conditions for two growing seasons in 2006-2007 and 2007-2008. The treatments included control (ambient day length and temperature); SD (8 h days) and ambient temperature; SD + Chilling (Ch) (18/12°C day/night temperatures); SD + Ch (10°C); SD + Ch (2°C); and, GA3 (10 ppm applied to the plants in November). In each growing season, the plug plants were planted in mid-August using bag culture. Yield, fruit weight, firmness, total soluble solids (SS), titratable acidity (TA), and SS/TA ratio were investigated. Considerable amounts of early yield (March and April) were recovered from ‘Sweet Charlie’; 116 and 72 g/plant in the first and second growing seasons, respectively. ‘Sweet Charlie’ also had consistently higher total yield than ‘Camarosa’ (457 vs. 400 g/plant in the 2006-2007 season and 446 vs. 406 g/plant in the 2007-2008 season). Treatment did not have a consistent effect on the fruit quality traits measured. The results indicated that although SD conditioning, Ch and GA3 treatments may have an effect on the total and early yield of strawberries, these effects may be cultivar-dependent and further regulated by environmental factors. Therefore, we propose that low-chilling varieties be utilized for early fruit production that can initiate flower buds in warmer, longer days.

scholarly journals Quantitative Trait Loci and Candidate Genes Associated with Photoperiod Sensitivity in Lettuce (Lactuca spp.)

2021 ◽  
Author(s):  
Rongkui Han ◽  
Dean Lavelle ◽  
Maria José Truco ◽  
Richard Michelmore
Keyword(s):  
Quantitative Trait Loci ◽  
Flowering Time ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Photoperiod Sensitivity ◽  
Short Day ◽  
Lactuca Serriola ◽  
Long Day ◽  
Trait Loci ◽  
Photoperiod Sensitive

Abstract Key message A population of lettuce that segregated for photoperiod sensitivity was planted under long-day and short-day conditions. Genetic mapping revealed two distinct sets of QTLs controlling daylength-independent and photoperiod-sensitive flowering time. Abstract The molecular mechanism of flowering time regulation in lettuce is of interest to both geneticists and breeders because of the extensive impact of this trait on agricultural production. Lettuce is a facultative long-day plant which changes in flowering time in response to photoperiod. Variations exist in both flowering time and the degree of photoperiod sensitivity among accessions of wild (Lactuca serriola) and cultivated (L. sativa) lettuce. An F6 population of 236 recombinant inbred lines (RILs) was previously developed from a cross between a late-flowering, photoperiod-sensitive L. serriola accession and an early-flowering, photoperiod-insensitive L. sativa accession. This population was planted under long-day (LD) and short-day (SD) conditions in a total of four field and screenhouse trials; the developmental phenotype was scored weekly in each trial. Using genotyping-by-sequencing (GBS) data of the RILs, quantitative trait loci (QTL) mapping revealed five flowering time QTLs that together explained more than 20% of the variation in flowering time under LD conditions. Using two independent statistical models to extract the photoperiod sensitivity phenotype from the LD and SD flowering time data, we identified an additional five QTLs that together explained more than 30% of the variation in photoperiod sensitivity in the population. Orthology and sequence analysis of genes within the nine QTLs revealed potential functional equivalents in the lettuce genome to the key regulators of flowering time and photoperiodism, FD and CONSTANS, respectively, in Arabidopsis.

Quantitative genetic analysis of flowering time in tomato

Genome ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 303-315 ◽  
Author(s):  
José M. Jiménez-Gómez ◽  
Carlos Alonso-Blanco ◽  
Alicia Borja ◽  
Germán Anastasio ◽  
Trinidad Angosto ◽  
...  
Keyword(s):  
Flowering Time ◽  
Solanum Species ◽  
Day Length ◽  
Leaf Number ◽  
Phenotypic Variance ◽  
Days To Flowering ◽  
Solanum Lycopersicum L ◽  
Close Relationship ◽  
Genetic Mechanisms ◽  
Trait Locus

Artificial selection of cultivated tomato ( Solanum lycopersicum L.) has resulted in the generation of early-flowering, day-length-insensitive cultivars, despite its close relationship to other Solanum species that need more time and specific photoperiods to flower. To investigate the genetic mechanisms controlling flowering time in tomato and related species, we performed a quantitative trait locus (QTL) analysis for flowering time in an F2 mapping population derived from S. lycopersicum and its late-flowering wild relative S. chmielewskii . Flowering time was scored as the number of days from sowing to the opening of the first flower (days to flowering), and as the number of leaves under the first inflorescence (leaf number). QTL analyses detected 2 QTLs affecting days to flowering, which explained 55.3% of the total phenotypic variance, and 6 QTLs for leaf number, accounting for 66.7% of the corresponding phenotypic variance. Four of the leaf number QTLs had not previously been detected for this trait in tomato. Colocation of some QTLs with flowering-time genes included in the genetic map suggests PHYB2, FALSIFLORA, and a tomato FLC-like sequence as candidate genes that might have been targets of selection during the domestication of tomato.

scholarly journals Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses

2020 ◽  
Author(s):  
Hongxu Dong ◽  
Lindsay V. Clark ◽  
Xiaoli Jin ◽  
Kossonou Anzoua ◽  
Larisa Bagmet ◽  
...  
Keyword(s):  
Flowering Time ◽  
High Latitude ◽  
Floral Induction ◽  
Day Treatment ◽  
Day Length ◽  
Close Relative ◽  
Short Day ◽  
Culm Length ◽  
Low Latitudes ◽  
Short Days

AbstractMiscanthus is a close relative of Saccharum and a potentially valuable genetic resource for improving sugarcane. Differences in flowering time within and between Miscanthus and Saccharum hinders intra- and interspecific hybridizations. A series of greenhouse experiments were conducted over three years to determine how to synchronize flowering time of Saccharum and Miscanthus genotypes. We found that day length was an important factor influencing when Miscanthus and Saccharum flowered. Sugarcane could be induced to flower in a central Illinois greenhouse using supplemental lighting to reduce the rate at which days shortened during the autumn and winter to 1 min d-1, which allowed us to synchronize the flowering of some sugarcane genotypes with Miscanthus genotypes primarily from low latitudes. In a complementary growth chamber experiment, we evaluated 33 Miscanthus genotypes, including 28 M. sinensis, 2 M. floridulus, and 3 M. ×giganteus collected from 20.9° S to 44.9° N for response to three day lengths (10 h, 12.5 h, and 15 h). High latitude-adapted M. sinensis flowered mainly under 15 h days, but unexpectedly, short days resulted in short, stocky plants that did not flower; in some cases, flag leaves developed under short days but heading did not occur. In contrast, for M. sinensis and M. floridulus from low latitudes, shorter day lengths typically resulted in earlier flowering, and for some low latitude genotypes, 15 h days resulted in no flowering. However, the highest ratio of reproductive shoots to total number of culms was typically observed for 12.5 h or 15 h days. Latitude of origin was significantly associated with culm length, and the shorter the days, the stronger the relationship. Nearly all entries achieved maximal culm length under the 15 h treatment, but the nearer to the equator an accession originated, the less of a difference in culm length between the short-day treatments and the 15 h day treatment. Under short days, short culms for high-latitude accessions was achieved by different physiological mechanisms for M. sinensis genetic groups from the mainland in comparison to those from Japan; for mainland accessions, the mechanism was reduced internode length, whereas for Japanese accessions the phyllochron under short days was greater than under long days. Thus, for M. sinensis, short days typically hastened floral induction, consistent with the expectations for a facultative short-day plant. However, for high latitude accessions of M. sinensis, days less than 12.5 h also signaled that plants should prepare for winter by producing many short culms with limited elongation and development; moreover, this response was also epistatic to flowering. Thus, to flower M. sinensis that originates from high latitudes synchronously with sugarcane, the former needs day lengths >12.5 h (perhaps as high as 15 h), whereas that the latter needs day lengths <12.5 h.

scholarly journals Identification of a Major and Stable QTL on Chromosome 5A Confers Spike Length in Wheat (Triticum aestivum L.)

2021 ◽  
Author(s):  
Guangsi Ji ◽  
Zhibin Xu ◽  
Xiaoli Fan ◽  
Qiang Zhou ◽  
Qin Yu ◽  
...  
Keyword(s):  
Bulked Segregant Analysis ◽  
Recombinant Inbred Lines ◽  
Physical Region ◽  
Triticum Aestivum L ◽  
Spike Length ◽  
Yield Potential ◽  
Nucleotide Polymorphisms ◽  
Specific Pcr ◽  
Chromosome 5A ◽  
Trait Locus

Abstract Spike length (SL) is the key determinant of plant architecture and yield potential. In this study, 193 recombinant inbred lines (RILs) derived from a cross between 13F10 and Chuanmai 42 (CM42) were evaluated for spike length in six environments. Sixty RILs consisting of 30 high and 30 low SLs were genotyped using the bulked segregant analysis exome sequencing (BSE-Seq) analysis for preliminary quantitative trait locus (QTL) mapping. A 6.69 Mb (518.43-525.12 Mb) region on chromosome 5AL was found to have a significant effect on the SL trait. Fifteen kompetitive allele specific PCR (KASP) markers were successfully converted from the single nucleotide polymorphisms (SNPs) in the SL target region. Combined with four novel simple sequence repeat (SSR) markers, a genetic linkage map spanning 21.159 cM was constructed. The mapping result confirmed the identity of a major and stable QTL named QSl.cib-5A in the targeted region that explained 7.88–26.60% of the phenotypic variation in SL. QSl.cib-5A was narrowed to a region of 4.84 cM interval corresponding to a 4.67 Mb (516.60-521.27 Mb) physical region in the Chinese Spring RefSeq v2.0 containing 17 high-confidence genes with 25 transcripts. In addition, this QTL exhibited pleiotropic effects on spikelet density (SD), with the phenotypic variances proportion ranging from 11.34–19.92%. This study provides a foundational step for cloning the QSl.cib-5A, which is involved in the regulation of spike morphology in common wheat.

scholarly journals Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 288
Author(s):  
Zhihui Guo ◽  
Meilan Xu ◽  
Hironori Nagano ◽  
Lindsay V. Clark ◽  
Erik J. Sacks ◽  
...  
Keyword(s):  
Gene Expression ◽  
Flowering Time ◽  
Heading Date ◽  
Biomass Accumulation ◽  
Initial Step ◽  
Day Length ◽  
Miscanthus Sinensis ◽  
Sequence Characterization ◽  
Mini Core Collection ◽  
Trait Locus

The optimal flowering time for bioenergy crop Miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in Miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensisGhd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species’ subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus.

Sign in / Sign up

Export Citation Format

Share Document