scholarly journals Toward the development of highly homozygous diploid potato lines using the self-compatibility controlling Sli gene

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 977-984 ◽  
Author(s):  
Chalermpol Phumichai ◽  
Motoyuki Mori ◽  
Akira Kobayashi ◽  
Osamu Kamijima ◽  
Kazuyoshi Hosaka
Keyword(s):  
Genetic Map ◽  
Average Rate ◽  
The Self ◽  
Aflp Markers ◽  
Diploid Potato ◽  
Average Heterozygosity ◽  
Diploid Clone ◽  
Self Compatibility ◽  
Homozygous Diploid ◽  
Sli Gene

Cultivated diploid potatoes (2n = 2x = 24) are self-incompatible, but can be altered to become self-compatible using the Sli gene. Previously, a diploid clone 97H32–6 was selfed up to S3 using the Sli gene. To explore the usefulness of the Sli gene for the production of highly homozygous diploid potatoes, 2 S4 families from the above 97H32-6 derived S3 lines (inbred series A) and 3 S5 families by continuous selfings from a different F1 (= S0) plant (inbred series B) were developed. The level of heterozygosity and the location of heterozygous loci on the genetic map were investigated using RFLP and AFLP markers. The average heterozygosity levels of the originally heterozygous loci decreased from 100% in S0 to 10.7% in S4 and 8.6% in S5 (inbred series A and B, respectively). The average rate of reduction in heterozygosity per generation (38.4% and 38.5% for inbred series A and B, respectively) was lower than the theoretically expected rate (50%). However, none of the loci or chromosome sections was exclusively heterozygous in the advanced self-progeny. Thus, highly homozygous and seed-propagated diploid potatoes could be obtained by repeated selfing using the Sli gene.Key words: Selfing, heterozygosity, diploid potato, Sli gene, Solanum.

CORRELATED RESPONSE IN BROWN SARSON

1970 ◽  
Vol 12 (4) ◽  
pp. 905-913 ◽  
Author(s):  
T. Swamy Rao
Keyword(s):  
Gamma Irradiation ◽  
Seed Set ◽  
Previous History ◽  
The Self ◽  
Correlated Response ◽  
History Of ◽  
Self Compatibility ◽  
Selection For ◽  
Direction Opposite ◽  
Brown Sarson

The correlated response with changes in self-compatibility in three varieties of brown sarson subjected to gamma irradiation was examined. Selection for improved seed set in the irradiated populations showed that substantial correlated response can result for a constellation of other characters in which the self-compatible and self-incompatible forms differ. The correlated response was in a direction opposite to that of the previous history of selection.

A High‐Density Soybean Genetic Map Based on AFLP Markers

Crop Science ◽  
1997 ◽  
Vol 37 (2) ◽  
pp. 537-543 ◽  
Author(s):  
Paul Keim ◽  
James M. Schupp ◽  
Steven E. Travis ◽  
Kathryn Clayton ◽  
Tong Zhu ◽  
...  
Keyword(s):  
Genetic Map ◽  
High Density ◽  
Aflp Markers

scholarly journals Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides ‘S9-2’

2001 ◽  
Vol 91 (11) ◽  
pp. 1069-1073 ◽  
Author(s):  
J. Zhang ◽  
M. Steenackers ◽  
V. Storme ◽  
S. Neyrinck ◽  
M. Van Montagu ◽  
...  
Keyword(s):  
Binding Site ◽  
Genetic Map ◽  
Positional Cloning ◽  
Populus Deltoides ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Aflp Markers ◽  
Interspecific Crosses ◽  
Leucine Rich Repeat ◽  
Starting Point

Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides ‘S9-2’. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

scholarly journals Rapid evolution of Wolbachia incompatibility types

2012 ◽  
Vol 279 (1746) ◽  
pp. 4473-4480 ◽  
Author(s):  
Olivier Duron ◽  
Jennifer Bernard ◽  
Célestine M. Atyame ◽  
Emilie Dumas ◽  
Mylène Weill
Keyword(s):  
Reproductive Success ◽  
Dynamic Process ◽  
Culex Pipiens ◽  
Cytoplasmic Incompatibility ◽  
Rapid Evolution ◽  
The Self ◽  
Isofemale Lines ◽  
Individual Host ◽  
Gradual Process ◽  
Self Compatibility

In most insects, the endosymbiont Wolbachia induces cytoplasmic incompatibility (CI), an embryonic mortality observed when infected males mate either with uninfected females or with females infected by an incompatible Wolbachia strain. Although the molecular mechanism of CI remains elusive, it is classically viewed as a modification–rescue model, in which a Wolbachia mod function disables the reproductive success of the sperm of infected males, unless eggs are infected and express a compatible resc function. The extent to which the modification–rescue model can predict highly complex CI pattern remains a challenging issue. Here, we show the rapid evolution of the mod–resc system in the Culex pipiens mosquito. We have surveyed four incompatible laboratory isofemale lines over 50 generations and observed in two of them that CI has evolved from complete to partial incompatibility (i.e. the production of a mixture of compatible and incompatible clutches). Emergence of the new CI types depends only on Wolbachia determinants and can be simply explained by the gain of new resc functions. Evolution of CI types in Cx. pipiens thus appears as a gradual process, in which one or several resc functions can coexist in the same individual host in addition to the ones involved in the self-compatibility. Our data identified CI as a very dynamic process. We suggest that ancestral and mutant Wolbachia expressing distinct resc functions can co-infect individual hosts, opening the possibility for the mod functions to evolve subsequently. This gives a first clue towards the understanding of how Wolbachia reached highly complex CI pattern in host populations.

The self-compatibility effect in graded thermoelectric cooler elements

2013 ◽  
Vol 210 (7) ◽  
pp. 1407-1417 ◽  
Author(s):  
Wolfgang Seifert ◽  
G. Jeffrey Snyder ◽  
Eric S. Toberer ◽  
Christophe Goupil ◽  
Knud Zabrocki ◽  
...  
Keyword(s):  
Compatibility Effect ◽  
The Self ◽  
Thermoelectric Cooler ◽  
Self Compatibility

scholarly journals Molecular Analysis of S-haplotypes in Peach, a Self-compatible Prunus Species

2006 ◽  
Vol 131 (6) ◽  
pp. 738-743 ◽  
Author(s):  
Attila Hegedüs ◽  
Zoltán Szabó ◽  
József Nyéki ◽  
Júlia Halász ◽  
Andrzej Pedryc
Keyword(s):  
Prunus Persica ◽  
Fragment Size ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein A ◽  
Synonymous Substitution ◽  
The Self ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Self Compatibility ◽  
Length Determination

The most commercially grown peach [Prunus persica (L.) Batsch.] cultivars do not require cross-pollination for reasonable fruit set; however, self-incompatibility is a well-known feature within the Prunoideae subfamily. Isoelectric focusing and native polyacrylamide gel electrophoresis of S-ribonucleases; PCR analyses of S-RNase and S-haplotype-specific F-box genes as well as DNA sequencing were carried out to survey the self-(in)compatibility allele pool and to uncover the nature of self-compatibility in peach. From 25 cultivars and hybrids with considerable diversity in phenotype and origin, only two S-haplotypes were detected. Allele identity could be checked by exact length determination of the PCR-amplified fragments and/or partial sequencing of the peach S1-, S2-, and Prunus davidiana (Carr.) Franch. S1-RNases. S-RNases of peach were detected to possess ribonuclease activity, and a single nucleotide polymorphism in the S1-RNase was shown, which represents a synonymous substitution and does not change the amino acid present at the position in the protein. A 700-bp fragment of the peach SFB gene was PCR-amplified, which is similar to the fragment size of functional Prunus L. SFBs. All data obtained in this study may support the contribution of genes outside the S-locus to the self-compatible phenotype of peaches.

scholarly journals S6-RNase Is a Marker for Self-compatibility in Loquat (Eriobotrya japonica Lindl.)

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1146-1149 ◽  
Author(s):  
Reut Niska ◽  
Martin Goldway ◽  
Doron Schneider
Keyword(s):  
Amino Acid ◽  
System Analysis ◽  
The Self ◽  
Eriobotrya Japonica ◽  
Amino Acid Similarity ◽  
Self Fertilization ◽  
Self Compatibility ◽  
Fertilization System ◽  
Selection Of

Loquat (Eriobotrya japonica Lindl.), a member of the Rosaceae, carries the RNase-dependent gametophytic self-incompatibility fertilization system. Analysis of S-RNase-allele content in the commercial loquat cultivars Avri, Yehuda, and Akko 1 revealed that each of them contains one different S-RNase allele—S2, S3, and S4, respectively, and one that they all share, S6. Although all four S-alleles were isolated in this work, only S6 was found to be novel. Amino acid similarity between the partial sequence of S6-RNase and other known loquat RNases (S1 to S4) ranged between 62% and 65% with highest similarity (83%) to the S110-allele of European pear (Pyrus communis). Determination of S-RNase-allele content in progeny of ‘Avri’, ‘Yehuda’, and ‘Akko 1’, obtained in an open-pollinated, mixed-cultivar orchard, revealed that all of the progeny derived from self-fertilization contained the S6 haplotype, indicating that a mutation in the S6 locus is responsible for the self-fertilization. However, sequencing of most of the S6-RNase gene (from C1 to C5) did not reveal any mutation and the alignment of the deduced amino acid sequence showed that it has the expected S-RNase primary and tertiary structural organization. Nonetheless, because it is apparent that the S6-RNase allele is linked to the self-compatibility trait, it could serve as a marker for early selection of self-compatible loquat cultivars.

scholarly journals Self-Compatibility Not Associated with Morphological or Genetic Diversity Reduction in Oil-Rewarding Calceolaria Species

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1377
Author(s):  
Maureen Murúa ◽  
Anahí Espíndola ◽  
Fernanda Pérez
Keyword(s):  
Genetic Diversity ◽  
Floral Biology ◽  
The Self ◽  
Floral Traits ◽  
Reproductive Assurance ◽  
Pollinator Attraction ◽  
Evolutionary Transitions ◽  
Allele Number ◽  
Self Compatibility ◽  
Study Species

One of the most common evolutionary transitions in angiosperms is the reproductive change from outcrossing to selfing, commonly associated with changes in floral biology and genetic diversity. Here, we aim to test whether self-compatibility leads to a reduction of floral traits and genetic diversity. For this, we experimentally estimate levels of self-compatibility, measure three floral traits and estimate four genetic diversity parameters using nine microsatellites in nine Calceolaria species. Our analysis indicated that four of the study species were self-incompatible. In addition, we found that self-compatible species did not show a reduction in floral traits size, but rather displayed larger corolla and elaiophore areas. Our analyses of genetic diversity identified larger allele number and observed heterozygosity in selfers than in outcrossers, but did not find larger inbreeding in the self-compatible species. Even though our results contradict our expectations, in the case of Calceolaria, their high dependence on only two genera of oil-bees puts the genus in a vulnerable reproductive position, probably facilitating the evolution of reproductive assurance mechanisms in the absence of pollinators. As a result, plants maintain their pollinator attraction traits while evolving the ability to self, possibly in a delayed way.

scholarly journals Expanded Genetic Map of Gibberella moniliformis (Fusarium verticillioides)

2002 ◽  
Vol 68 (4) ◽  
pp. 1972-1979 ◽  
Author(s):  
James E. Jurgenson ◽  
Kurt A. Zeller ◽  
John F. Leslie
Keyword(s):  
Genetic Map ◽  
Fragment Length ◽  
Fusarium Verticillioides ◽  
Pathogenic Fungi ◽  
Aflp Markers ◽  
Crop Species ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Further Development ◽  
Gibberella Moniliformis

ABSTRACT Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food- and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processors. The dissection of important biological characters in this fungus has been hampered by the lack of a uniformly dense genetic map. The existing restriction fragment length polymorphism-based map contains significant gaps, making it difficult to routinely locate biologically important genes, such as those involved in pathogenicity or mycotoxin production, with precision. We utilized amplified fragment length polymorphisms (AFLPs) to saturate the existing genetic map and added 486 AFLP markers to the ∼150 markers on the existing map. The resulting map has an average marker interval of 3.9 map units and averages ∼21 kb/map unit. The additional markers expanded the map from 1,452 to 2,188 map units distributed across 12 chromosomes. The maximum distance between adjacent markers is 29 map units. We identified AFLP markers less than 1 map unit from the mating type (MAT) locus and 2.5 map units from the spore killer (SK) locus; eight AFLP markers map within 8.5 units of the FUM1 (fumonisin biosynthetic) locus. The increased saturation of this map will facilitate further development of G. moniliformis as a model system for the genetic and population genetic studies of related, but less genetically tractable, plant pathogenic fungi.

Sign in / Sign up

Export Citation Format

Share Document