Erratum: A direct repeat sequence associated with the centromeric retrotransposons in wheat

Genome ◽  
2004 ◽  
Vol 47 (5) ◽  
pp. 998-998
Author(s):  
Hidetako Ito ◽  
Shuhei Nasuda ◽  
Takeshi R Endo
Keyword(s):  
Direct Repeat ◽  
Repeat Sequence ◽  
Direct Repeat Sequence

scholarly journals In the Staphylococcus aureus Two-Component System sae, the Response Regulator SaeR Binds to a Direct Repeat Sequence and DNA Binding Requires Phosphorylation by the Sensor Kinase SaeS

2010 ◽  
Vol 192 (8) ◽  
pp. 2111-2127 ◽  
Author(s):  
Fei Sun ◽  
Chunling Li ◽  
Dowon Jeong ◽  
Changmo Sohn ◽  
Chuan He ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dna Binding ◽  
Response Regulator ◽  
Direct Repeat ◽  
Repeat Sequence ◽  
Sensor Kinase ◽  
Two Component System ◽  
Component System ◽  
Direct Repeat Sequence ◽  
Two Component

ABSTRACT Staphylococcus aureus uses the SaeRS two-component system to control the expression of many virulence factors such as alpha-hemolysin and coagulase; however, the molecular mechanism of this signaling has not yet been elucidated. Here, using the P1 promoter of the sae operon as a model target DNA, we demonstrated that the unphosphorylated response regulator SaeR does not bind to the P1 promoter DNA, while its C-terminal DNA binding domain alone does. The DNA binding activity of full-length SaeR could be restored by sensor kinase SaeS-induced phosphorylation. Phosphorylated SaeR is more resistant to digestion by trypsin, suggesting conformational changes. DNase I footprinting assays revealed that the SaeR protection region in the P1 promoter contains a direct repeat sequence (GTTAAN6GTTAA [where N is any nucleotide]). This sequence is critical to the binding of phosphorylated SaeR. Mutational changes in the repeat sequence greatly reduced both the in vitro binding of SaeR and the in vivo function of the P1 promoter. From these results, we concluded that SaeR recognizes the direct repeat sequence as a binding site and that binding requires phosphorylation by SaeS.

scholarly journals The Macrolide-Lincosamide-Streptogramin B Resistance Determinant from Clostridium difficile 630 Contains Two erm(B) Genes

2000 ◽  
Vol 44 (2) ◽  
pp. 411-413 ◽  
Author(s):  
Kylie A. Farrow ◽  
Dena Lyras ◽  
Julian I. Rood
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Perfringens ◽  
Direct Repeat ◽  
Repeat Sequence ◽  
Resistance Determinant ◽  
Direct Repeat Sequence

ABSTRACT The ErmB macrolide-lincosamide-streptogramin B (MLS) resistance determinant from Clostridium difficile 630 contains two copies of an erm(B) gene, separated by a 1.34-kb direct repeat also found in an Erm(B) determinant from Clostridium perfringens. In addition, both erm(B) genes are flanked by variants of the direct repeat sequence. This genetic arrangement is novel for an ErmB MLS resistance determinant.

Hepatitis B virus direct repeat sequence: Imino proton exchange rates and distance and torsion angle restraints from NMR

Biochemistry ◽  
1994 ◽  
Vol 33 (2) ◽  
pp. 427-438 ◽  
Author(s):  
Karl D. Bishop ◽  
Forrest J. H. Blocker ◽  
William Egan ◽  
Thomas L. James
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Exchange Rates ◽  
Torsion Angle ◽  
Direct Repeat ◽  
Repeat Sequence ◽  
Proton Exchange ◽  
Imino Proton ◽  
Direct Repeat Sequence ◽  
B Virus

scholarly journals Engineering the Direct Repeat Sequence of crRNA for Optimization of FnCpf1-Mediated Genome Editing in Human Cells

2018 ◽  
Vol 26 (11) ◽  
pp. 2650-2657 ◽  
Author(s):  
Li Lin ◽  
Xiubin He ◽  
Tianyuan Zhao ◽  
Lingkai Gu ◽  
Yeqing Liu ◽  
...  
Keyword(s):  
Genome Editing ◽  
Direct Repeat ◽  
Repeat Sequence ◽  
Human Cells ◽  
Direct Repeat Sequence

scholarly journals The Upstream Direct Repeat Sequence of Prague A Rous Sarcoma Virus Is Deficient in Mediating Efficient Gag Assembly and Particle Release

Virology ◽  
1998 ◽  
Vol 247 (1) ◽  
pp. 86-96 ◽  
Author(s):  
Samantha B. Simpson ◽  
Wei Guo ◽  
Stanley C. Winistorfer ◽  
Rebecca C. Craven ◽  
C.Martin Stoltzfus
Keyword(s):  
Rous Sarcoma Virus ◽  
Direct Repeat ◽  
Repeat Sequence ◽  
Particle Release ◽  
Direct Repeat Sequence ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Gag Assembly

A Direct Repeat Sequence at theRasgrf1Locus and Imprinted Expression

Genomics ◽  
1999 ◽  
Vol 55 (2) ◽  
pp. 194-201 ◽  
Author(s):  
R.Scott Pearsall ◽  
C. Plass ◽  
M.A. Romano ◽  
M.D. Garrick ◽  
H. Shibata ◽  
...  
Keyword(s):  
Direct Repeat ◽  
Repeat Sequence ◽  
Direct Repeat Sequence
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