Wheat EST sequence assembly facilitates comparison of gene contents among plant species and discovery of novel genes

Genome ◽  
2005 ◽  
Vol 48 (2) ◽  
pp. 197-206 ◽  
Author(s):  
Jiro Hattori ◽  
Thérèse Ouellet ◽  
Nicholas A Tinker
Keyword(s):  
Plant Species ◽  
Expressed Sequence Tag ◽  
Draft Genome ◽  
Sequence Assembly ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Novel Genes ◽  
Cereal Genome ◽  
Computational Resources ◽  
Expressed Sequence

Using a strategy requiring only modest computational resources, wheat expressed sequence tag (EST) sequences from various sources were assembled into contigs and compared with a nonredundant barley sequence assembly, with ESTs, with complete draft genome sequences of rice and Arabidopsis thaliana, and with ESTs from other plant species. These comparisons indicate that (i) wheat sequences available from public sources represent a substantial proportion of the diversity of wheat coding sequences, (ii) prediction of open reading frames in the whole genome sequence improves when supplemented with EST information from other species, (iii) a substantial number of candidates for novel genes that are unique to wheat or related species can be identified, and (iv) a smaller number of genes can be identified that are common to monocots and dicots but absent from Arabidopsis. The sequences in the last group may have been lost from Arabidopsis after descendance from a common ancestor. Examples of potential novel wheat genes and Triticeae-specific genes are presented.Key words: small grain cereal, genome, wheat, barley, bioinformatics.

Survey of a cDNA library from the turkey (Meleagris gallopavo)

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 12-17 ◽  
Author(s):  
L D Chaves ◽  
J A Rowe ◽  
K M Reed
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Expressed Sequence Tags ◽  
Expressed Sequence Tag ◽  
Meleagris Gallopavo ◽  
Whole Genome Sequence ◽  
Snp Discovery ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Important Species ◽  
Expressed Sequence

Genome characterization and analysis is an imperative step in identifying and selectively breeding for improved traits of agriculturally important species. Expressed sequence tags (ESTs) represent a transcribed portion of the genome and are an effective way to identify genes within a species. Downstream applications of EST projects include DNA microarray construction and interspecies comparisons. In this study, 694 ESTs were sequenced and analyzed from a library derived from a 24-day-old turkey embryo. The 437 unique sequences identified were divided into 76 assembled contigs and 361 singletons. The majority of significant comparative matches occurred between the turkey sequences and sequences reported from the chicken. Whole genome sequence from the chicken was used to identify potential exon–intron boundaries for selected turkey clones and intron-amplifying primers were developed for sequence analysis and single nucleotide polymorphism (SNP) discovery. Identified SNPs were genotyped for linkage analysis on two turkey reference populations. This study significantly increases the number of EST sequences available for the turkey.Key words: turkey, cDNA, expressed sequence tag, single nucleotide polymorphism.

Identification of novel genes from Entamoeba histolytica by expressed sequence tag analysis

Gene ◽  
1996 ◽  
Vol 181 (1-2) ◽  
pp. 113-116 ◽  
Author(s):  
A. Azam ◽  
J. Paul ◽  
D. Sehgal ◽  
J. Prasad ◽  
S. Bhattacharya ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Expressed Sequence Tag ◽  
Novel Genes ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

scholarly journals Draft Whole-Genome Sequence of “Candidatus Liberibacter asiaticus” Strain TX2351 Isolated from Asian Citrus Psyllids in Texas, USA

2017 ◽  
Vol 5 (15) ◽  
Author(s):  
Madhurababu Kunta ◽  
Zheng Zheng ◽  
Fengnian Wu ◽  
John V. da Graca ◽  
Jong-Won Park ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
South Texas ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Rna Genes ◽  
Reading Frames

ABSTRACT We report here the draft genome sequence of “Candidatus Liberibacter asiaticus” strain TX2351, collected from Asian citrus psyllids in south Texas, USA. The TX2351 genome has a size of 1,252,043 bp, a G+C content of 36.5%, 1,184 predicted open reading frames, and 52 RNA genes.

scholarly journals Draft Whole-Genome Sequence of “Candidatus Liberibacter asiaticus” Strain TX1712 from Citrus in Texas

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
W. Cai ◽  
Z. Yan ◽  
J. Rascoe ◽  
M. J. Stulberg
Keyword(s):  
Genome Sequence ◽  
Reference Genome ◽  
Draft Genome ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Candidatus Liberibacter Asiaticus ◽  
Content Type ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Reading Frames

The draft genome sequence of “Candidatus Liberibacter asiaticus” strain TX1712, obtained from a Texas citrus tree, is reported here. Strain TX1712 has a draft genome size of 1,203,333 bp, a G+C content of 36.4%, 1,230 predicted open reading frames, and 41 RNAs and comprises 97.4% of the psy62 reference genome.

scholarly journals Development and Characterization of Expressed Sequence Tag-Derived Microsatellite Markers for the Wheat Stem Rust Fungus Puccinia graminis f. sp. tritici

2009 ◽  
Vol 99 (3) ◽  
pp. 282-289 ◽  
Author(s):  
S. Zhong ◽  
Y. Leng ◽  
T. L. Friesen ◽  
J. D. Faris ◽  
L. J. Szabo
Keyword(s):  
Ssr Markers ◽  
Stem Rust ◽  
Expressed Sequence Tag ◽  
Rust Fungus ◽  
Draft Genome ◽  
Puccinia Graminis ◽  
Wheat Stem Rust ◽  
Ssr Loci ◽  
Expressed Sequence

Puccinia graminis f. sp. tritici is the causal agent of stem rust disease in wheat. The rust fungus has caused devastating disease epidemics throughout history and is still posing a potential threat to wheat production in some regions of the world due to the appearance of new races. To develop microsatellite or simple sequence repeat (SSR) markers for use in population genetics studies, a total of 60,579 expressed sequence tag (EST) sequences (reads) generated from P. graminis f. sp. tritici were screened for tandemly repeated di- and tri-nucleotide units using a bioinformatics approach and 708 unisequences containing putative SSR loci with six or more repeat units were identified. Flanking primers were designed for 384 unique SSR loci, which mapped to different locations of the draft genome sequence of the fungus. Of the 384 primer pairs tested, 72 EST-SSR markers were eventually developed, which showed polymorphism among 19 isolates of P. graminis f. sp. tritici and 4 isolates of P. graminis f. sp. secalis evaluated. Thirty-two of the SSR loci were also evaluated in three other rust fungi (P. triticina, P. hordei, and P. coronata f. sp. hordei) for cross-species transferability. These SSR markers derived from ESTs will be useful for characterization of population structures and for gene mapping in P. graminis.

scholarly journals De Novo Whole-Genome Sequence of Xylella fastidiosa subsp. multiplex Strain BB01 Isolated from a Blueberry in Georgia, USA

2017 ◽  
Vol 5 (6) ◽  
Author(s):  
Christopher Van Horn ◽  
Chung-Jan Chang ◽  
Jianchi Chen
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Xylella Fastidiosa ◽  
Draft Genome ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Rna Genes ◽  
Reading Frames

ABSTRACT This study reports a de novo-assembled draft genome sequence of Xylella fastidiosa subsp. multiplex strain BB01 causing blueberry bacterial leaf scorch in Georgia, USA. The BB01 genome is 2,517,579 bp, with a G+C content of 51.8%, 2,943 open reading frames (ORFs), and 48 RNA genes.

scholarly journals Whole-Genome Sequence of Bacillus subtilis WS1A, a Promising Fish Probiotic Strain Isolated from Marine Sponge of the Bay of Bengal

2020 ◽  
Vol 9 (39) ◽  
Author(s):  
M. Mahbubur Rahman ◽  
Sulav Indra Paul ◽  
Tasmina Akter ◽  
Alfred Chin Yen Tay ◽  
M. Javed Foysal ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Probiotic Strain ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Content Type ◽  
Aeromonas Veronii ◽  
Reading Frames

ABSTRACT This study reports the draft genome sequence of a promising fish probiotic, Bacillus subtilis strain WS1A, that possesses antimicrobial activity against Aeromonas veronii and suppressed motile Aeromonas septicemia in Labeo rohita. The de novo assembly resulted in an estimated chromosome size of 4,148,460 bp, with 4,288 open reading frames.

scholarly journals Draft Whole-Genome Sequence of a “Candidatus Liberibacter asiaticus” Strain from Yunnan, China

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Yanling Chen ◽  
Tao Li ◽  
Zheng Zheng ◽  
Meirong Xu ◽  
Xiaoling Deng
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Content Type ◽  
A Genome ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Rna Genes ◽  
Reading Frames

The draft genome sequence of “Candidatus Liberibacter asiaticus” strain YNJS7C, isolated from a navel orange tree in Yunnan Province, China, is presented here. The YNJS7C strain has a genome size of 1,258,986 bp, with a G+C content of 36.6%, 1,174 predicted open reading frames, and 53 RNA genes.

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