A comparison of sequence-based polymorphism and haplotype content in transcribed and anonymous regions of the barley genome

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 389-398 ◽  
Author(s):  
Joanne Russell ◽  
Allan Booth ◽  
John Fuller ◽  
Brian Harrower ◽  
Peter Hedley ◽  
...  
Keyword(s):  
Nucleotide Diversity ◽  
Wild Barley ◽  
Genomic Ssrs ◽  
Dna Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Change ◽  
Single Nucleotide ◽  
Single Nucleotide Change ◽  
Haplotype Data ◽  
Fertile Crescent

Direct estimates of sequence diversity provides an abundant source of DNA polymorphisms based on single nucleotide polymorphisms (SNPs). The frequency and distribution of nucleotide diversity within 23 genes associated with grain germination in barley were determined in a sample of accessions representing European cultivars, landraces, and wild barley accessions from throughout the fertile crescent. The overall nucleotide diversity ranged from 0.0021 to 0.0189 with a single nucleotide change being detected every 78 bp and insertion–deletion events being observed every 680 bp. Within the cultivated (H. vulgare) genepool, a small number of haplotypes were detected, the total number of haplotypes observed in H. spontaneum was almost double that detected in H. vulgare (46 and 26, respectively). Distinct haplotypes were observed in the H. spontaneum and landrace genepools, which are highly divergent from H. vulgare. A comparison of SNP-based haplotype data with EST-derived SSRs and genomic SSRs revealed a similar trend of decreasing variability in the cultivated genepool. However, the number of unique alleles identified in the cultivated sample was much greater with genomic SSRs (18%) compared with only 2.1% for SNPs and 3.8% for EST-derived SSRs. The potential utility of SNPs and EST-derived SSRs for association mapping in barley is discussed.Key words: SNPs, haplotype, SSRs, barley.

scholarly journals Single-Nucleotide Polymorphisms in Soybean

Genetics ◽  
2003 ◽  
Vol 163 (3) ◽  
pp. 1123-1134 ◽  
Author(s):  
Y L Zhu ◽  
Q J Song ◽  
D L Hyten ◽  
C P Van Tassell ◽  
L K Matukumalli ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genomic Dna ◽  
Nucleotide Diversity ◽  
Genomic Sequence ◽  
Haplotype Diversity ◽  
Pcr Primers ◽  
Dna Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Cultivated Soybean

Abstract Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and random genomic sequence; (2) to characterize haplotype variation in these sequences; and (3) to provide initial estimates of linkage disequilibrium (LD) in soybean. Approximately 28.7 kbp of coding sequence, 37.9 kbp of noncoding perigenic DNA, and 9.7 kbp of random noncoding genomic DNA were sequenced in each of 25 diverse soybean genotypes. Over the >76 kbp, mean nucleotide diversity expressed as Watterson’s θ was 0.00097. Nucleotide diversity was 0.00053 and 0.00111 in coding and in noncoding perigenic DNA, respectively, lower than estimates in the autogamous model species Arabidopsis thaliana. Haplotype analysis of SNP-containing fragments revealed a deficiency of haplotypes vs. the number that would be anticipated at linkage equilibrium. In 49 fragments with three or more SNPs, five haplotypes were present in one fragment while four or less were present in the remaining 48, thereby supporting the suggestion of relatively limited genetic variation in cultivated soybean. Squared allele-frequency correlations (r2) among haplotypes at 54 loci with two or more SNPs indicated low genome-wide LD. The low level of LD and the limited haplotype diversity suggested that the genome of any given soybean accession is a mosaic of three or four haplotypes. To facilitate SNP discovery and the development of a transcript map, subsets of four to six diverse genotypes, whose sequence analysis would permit the discovery of at least 75% of all SNPs present in the 25 genotypes as well as 90% of the common (frequency >0.10) SNPs, were identified.

scholarly journals Identification of genome-wide single-nucleotide polymorphisms among geographically diverse radish accessions

DNA Research ◽  
2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Hiroto Kobayashi ◽  
Kenta Shirasawa ◽  
Nobuko Fukino ◽  
Hideki Hirakawa ◽  
Takashi Akanuma ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Southeast Asia ◽  
East Asia ◽  
Near East ◽  
Dna Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
South And Southeast Asia ◽  
Snp Data ◽  
Genome Wide

Abstract Radish (Raphanus sativus L.) is cultivated around the world as a vegetable crop and exhibits diverse morphological and physiological features. DNA polymorphisms are responsible for differences in traits among cultivars. In this study, we determined genome-wide single-nucleotide polymorphisms (SNPs) among geographically diverse radish accessions using the double-digest restriction site-associated DNA sequencing (ddRAD-Seq) method. A total of 52,559 SNPs was identified in a collection of over 500 radish accessions (cultivated and wild) from East Asia, South and Southeast Asia, and the Occident and Near East. In addition, 2,624 SNP sites without missing data (referred to as common SNP sites) were identified among 510 accessions. Genetic diversity analyses, based on the common SNP sites, divided the cultivated radish accessions into four main groups, each derived from four geographical areas (Japan, East Asia, South and Southeast Asia, and the Occident and Near East). Furthermore, we discuss the origin of cultivated radish and its migration from the West to East Asia. SNP data generated in this work will facilitate further genetic studies on the radish breeding and production of DNA markers.

Novel single-nucleotide change inGYP*Ain a person who made an alloantibody to a new high-prevalence MNS antigen called ENEV

Transfusion ◽  
2010 ◽  
Vol 50 (4) ◽  
pp. 856-860 ◽  
Author(s):  
Randall W. Velliquette ◽  
Zong Hu ◽  
Christine Lomas-Francis ◽  
Kim Hue-Roye ◽  
Jean L. Allen ◽  
...  
Keyword(s):  
Nucleotide Change ◽  
Single Nucleotide ◽  
Single Nucleotide Change ◽  
High Prevalence

scholarly journals Identification of a single nucleotide change in a mutant gene for hypoxanthine-guanine phosphoribosyltransferase (HPRTAnn Arbor)

1988 ◽  
Vol 79 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Shin Fujimori ◽  
Yuji Hidaka ◽  
Beverly L. Davidson ◽  
Thomas D. Palella ◽  
William N. Kelley
Keyword(s):  
Mutant Gene ◽  
Nucleotide Change ◽  
Single Nucleotide ◽  
Single Nucleotide Change ◽  
Hypoxanthine Guanine Phosphoribosyltransferase
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