Interspecific androgenetic restoration of rosy barb using cadaveric sperm

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 66-73 ◽  
Author(s):  
S Kirankumar ◽  
T J Pandian
Keyword(s):  
Embryonic Development ◽  
Thermal Shock ◽  
Uv Irradiation ◽  
Sexual Maturity ◽  
Gonadosomatic Index ◽  
Sperm Concentration ◽  
Nuclear Genome ◽  
Time Sequence ◽  
Fertilizing Ability ◽  
Alien Cytoplasm

Interspecific androgenetic rosy barb (Puntius conchonius) was generated using its cadaveric (-20 °C) or fresh sperm to activate nuclear genome inactivated oocytes of gray tiger barb (Puntius tetrazona). UV irradiation was used to inactivate nuclear genome of tiger barb oocytes. Thermal shock restored diploidy of rosy barb in the oocytes of tiger barb. Survival of androgenotes was 14% or 7% when fresh or cadaveric sperm was used. The diploid or haploid nuclear genome of rosy barb, individually or jointly with that of tiger barb, regulated the time sequence of embryonic development in an alien cytoplasm of tiger barb oocytes. Androgenetic males (Y2Y2) attained sexual maturity earlier and had significantly higher gonadosomatic index and sperm concentration, albeit suffering a slight decrease in fertilizing ability. Conversely, androgenetic females (X2X2) suffered extended interspawning period, reduced fecundity, and poor hatchability of their progenies. These results are discussed with respect to their significance for conservation biology.Key words: nuclear genome inactivation, tiger barb, cadaveric sperm, rosy barb, interspecific androgenotes, Tc1 transposon.

scholarly journals Seasonal variations in the population biology of Salmostoma bacaila (Cyprinidae) from a tributary of the Payra River, Bangladesh

2019 ◽  
pp. 113-119
Author(s):  
Ahamed Ahamed ◽  
Faruque Ahmed Zoarder ◽  
Jun Ohtomi
Keyword(s):  
Sex Ratio ◽  
Seasonal Variations ◽  
Population Biology ◽  
Sexual Maturity ◽  
Condition Factor ◽  
Gonadosomatic Index ◽  
Spawning Season ◽  
Frequency Distributions ◽  
Size At Sexual Maturity ◽  
Management And Conservation

Some biological parameters of Salmostoma bacaila – including sex ratio, length-frequency distributions (LFDs), size at sexual maturity, spawning season, length-weight relationships (LWRs) and condition factor – were studied. Samples were collected seasonally during June 2017 to May 2018 from a tributary of the Payra River. The overall sex ratio was significantly different from the expected value of 1:1 (p < 0.001), in favour of male specimens. Females were significantly larger than males. Size at sexual maturity was estimated at 7.6~7.7 cm total length. Seasonal variations in the gonadosomatic index (GSI) indicate that the main spawning season is from spring to summer. The LWRs showed negative allometric growth in both sexes, but with clear seasonal variation. Fulton’s condition factor varied in both sexes and was attributed to variations in GSI with maturity. The fin¬dings of this study will be helpful for management and conservation of S. bacaila populations.

scholarly journals Reproductive indicators of the endemic species Astyanax bifasciatus (Teleostei: Characidae) in a tributary of the Lower Iguaçu River Basin, Brazil

2019 ◽  
Vol 41 ◽  
pp. e47720
Author(s):  
Elton Celton de Oliveira ◽  
Alexandre Augusto Auache-Filho ◽  
Denis Damasio ◽  
Nédia de Castilhos Ghisi ◽  
Maria Antônia Michels-Souza
Keyword(s):  
River Basin ◽  
Fish Species ◽  
Sexual Maturity ◽  
Gonadosomatic Index ◽  
Reproductive Period ◽  
Maturity Stage ◽  
Reproductive Process ◽  
Maturation Stages ◽  
High Degree ◽  
Iguaçu River

The reproductive process promotes morphophysiological and behavioral changes in fish species throughout their life cycle. Its success is vital to define their resilience in the environment. This study aimed to evaluate the reproductive biology of the endemic fish species Astyanax bifasciatus in a tributary of the Lower Iguaçu River Basin, Paraná, Brazil. Fish were collected monthly at four sites along the Jirau Alto River in the city of Dois Vizinhos from October 2015 to September 2016. A standardized catch effort with gill nets and fish traps was used. In the laboratory, standard length and total weight were recorded. After anesthesia, the animals were sectioned to macroscopically determine the sex, sexual maturity stage, and presence of celomatic fat. The gonads and liver were removed to determine the gonadosomatic and hepatosomatic indexes, respectively. A total of 160 individuals (104 females and 56 males) were used. The gonadosomatic index, frequency of the gonadal maturation stages, and condition factor showed a long reproductive period with two investment cycles for both sex. Length at first sexual maturity was 4.57 cm for females and 3.56 cm for males. The reproductive data corroborate the generalist profile of the species and demonstrate a high degree of adaptive capacity, even in smaller tributaries.

scholarly journals Reproductive biology of a tetragonopterinae (osteichthyes, characidae) of the Ubatiba fluvial system, Maricá - RJ

1999 ◽  
Vol 42 (4) ◽  
Author(s):  
Rosana Mazzoni ◽  
Jaqueline Petito
Keyword(s):  
Sexual Dimorphism ◽  
Reproductive Biology ◽  
Sexual Maturity ◽  
Gonadosomatic Index ◽  
Oocyte Diameter ◽  
Fluvial System ◽  
Development Stages ◽  
Maturation Stages ◽  
Mean Size ◽  
Reproduction Period

Aspects of the reproductive biology of one Tetragonopterinae was assessed in the Ubatiba river - Maricá / RJ. Length structure and mean size suggested sexual dimorphism, females reaching larger sizes than males. The onset of sexual maturity occured at 2.8cm for both the sexes. Fecundity, determined on ripe ovaries, showed positive correlation to females size and weight and varied from 595 to 5520 for 3.9cm and 6.2cm lengths respectively. The spawning type, defined as total, was determined by the oocyte diameter frequency from ovaries in different development stages. The frequency distribution of ovarian/testis maturation stages, associated with the temporal variation of the gonadosomatic index, suggested that the reproduction period was prolonged along the whole annual cycle.

scholarly journals Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1329
Author(s):  
Michele Di Iorio ◽  
Giusy Rusco ◽  
Roberta Iampietro ◽  
Lucia Maiuro ◽  
Achille Schiavone ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
In Vivo Test ◽  
Sperm Analysis ◽  
Fertilizing Ability ◽  
Vitro Analysis ◽  
Hatching Rates

This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of three insemination doses (250, 400 and 600 × 106 sperm/hen). Pooled semen samples were diluted with Tselutin or Lake extender which contained 20% of dimethylsulfoxide and 1 mM of Ficoll at final sperm concentration of 3 × 109 sperm/mL. Thereafter, semen was packaged into straws and frozen on liquid nitrogen. The post-thaw sperm quality was evaluated considering motility (computer-aided sperm analysis—CASA system) and membrane integrity (flow cytometry). Significantly higher values of progressive motility and some kinetic parameters in semen frozen with Lake were found. When we compared the extenders in vivo, no significant effects were detected, whilst sperm concentration significantly affected both fertility and hatching rates, with the best results obtained with the sperm concentration of 400 × 106 sperm/hen. From the results obtained, it emerged that the extender type only affected sperm motility characteristics, not the fertilizing ability of frozen-thawed semen, while inseminating dose markedly affected fertility and hatching rates.

scholarly journals Reproductive biology and embryonic development of Centroscymnus coelolepis in Portuguese mainland waters

2003 ◽  
Vol 60 (6) ◽  
pp. 1335-1341 ◽  
Author(s):  
Ana Veríssimo ◽  
Leonel Gordo ◽  
Ivone Figueiredo
Keyword(s):  
Sex Ratio ◽  
Embryonic Development ◽  
Reproductive Biology ◽  
Sexual Maturity ◽  
Sampling Period ◽  
Yolk Sac ◽  
Pregnant Female ◽  
Reproductive Parameters ◽  
Reproductive Characteristics ◽  
Total Length

Abstract A total of 871 females (76–122 cm) and 86 males (68–100 cm) of Centroscymnus coelolepis caught in Portuguese mainland waters were examined for reproductive characteristics. One hermaphroditic individual of 95 cm was found. Males were few in numbers and dominated by immature specimens. Female length at first sexual maturity was 98.5 cm. Mean ovarian and uterine fecundity were 13.2 oocytes per female and 9.9 embryos per pregnant female, respectively. Total length of embryos with completely absorbed external yolk sacs ranged between 233 and 300 mm. Sex ratio (F/M) of embryos with completely absorbed external yolk sac was 0.9. All stages of reproduction were found during the 12-month sampling period. Results were compared with those from other regions. A hypothesis is formulated on the existence of several regional populations based on morphometric and reproductive parameters observed in different areas.

scholarly journals Temporal Dynamics of Reproduction inHemiramphus brasiliensis(Osteichthyes: Hemiramphidae)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mônica Rocha de Oliveira ◽  
Sathyabama Chellappa
Keyword(s):  
Coastal Waters ◽  
Sexual Maturity ◽  
Temporal Dynamics ◽  
Gonadosomatic Index ◽  
Gonad Development ◽  
Reproductive Period ◽  
Northeastern Brazil ◽  
Histological Studies ◽  
Multiple Spawner ◽  
Maturation Stages

The reproductive aspects ofHemiramphus brasiliensiswere analyzed with a view to verify the temporal dynamics of reproduction. This paper presents data on sex ratio, length at first sexual maturity, macroscopic and histological aspects of gonad development, gonadosomatic index (GSI), reproductive period, and fecundity ofH. brasiliensis. The fishes were captured from the coastal waters of Rio Grande do Norte, northeastern Brazil. Females of this species predominated in the sampled population and were larger in size than the males. The length at the first sexual maturation of males was 20.8 cm and that of females was 21.5 cm. The macroscopic characteristics of the gonads indicated four maturation stages. Histological studies of gonads ofH. brasiliensisshowed six phases of oocyte development and four phases of spermatocyte development. The batch fecundity of this species was 1153 (±258.22) mature oocytes for 50 g body weight of female. The microscopic characteristics of gonad development indicate thatH. brasiliensisis a multiple spawner, presenting a prolonged reproductive period during the whole year, with a peak in the month of April, and is considered as an opportunistic strategist.

scholarly journals Changes in levels of major yolk protein in the coelomic fluid and gonad during the reproductive cycle in wild sea urchins,Mesocentrotus nudus

2017 ◽  
Author(s):  
Kazuhiro Ura ◽  
Narumi Takei ◽  
Ichiro Higuchi ◽  
Tomoharu Yuhi ◽  
Osamu Nishimiya ◽  
...  
Keyword(s):  
Reproductive Cycle ◽  
Sexual Maturity ◽  
Sea Urchins ◽  
Gonadosomatic Index ◽  
Yolk Protein ◽  
Coelomic Fluid ◽  
Mature Stage ◽  
Positive Correlation ◽  
Mesocentrotus Nudus ◽  
Quantitative Changes

AbstractBoth female and male sea urchins accumulate the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before gametogenesis, and MYP is the most abundant protein in the coelomic fluid of both sexes. In females, MYP in the coelomic fluid is taken up by the nutritive phagocytes and transported to the growing oocytes. This study examined quantitative changes of MYP in the coelomic fluid of both sexes during the reproductive cycle of wild sea urchins,Mesocentrotus nudus. Levels of MYP in the coelomic fluid of females increased and reached a peak at the histological pre-mature stage of gonad activity (i.e. Stage 3), and positive correlation between the MYP level and the gonadosomatic index (GSI) was observed. In male sea urchins the level of MYP in the coelomic fluid increased at the pre-mature stage, though positive correlation between the MYP level and GSI was not observed. These results indicate that MYP in the coelomic fluid is suitable as a biomarker of the onset and progression of sexual maturity in female sea urchins.

scholarly journals Reproductive biology of Penaeus kerathurus (Forskål, 1775) (Decapoda: Penaeidae) in the Sea of Marmara, Turkey

2021 ◽  
Vol 50 (1) ◽  
pp. 33-37
Author(s):  
Mukadder Arslan Ihsanoglu ◽  
Ismail Burak Daban ◽  
Ali Işmen ◽  
Koray Cabbar ◽  
Cahide Çiğdem Yığin
Keyword(s):  
Reproductive Biology ◽  
Sexual Maturity ◽  
Carapace Length ◽  
Gonadosomatic Index ◽  
Mediterranean Ecosystem ◽  
Sea Of Marmara ◽  
Important Species ◽  
Spawning Period ◽  
Male Ratio ◽  
First Reproduction

Abstract One of the economically important species is Penaeus kerathurus (Forskål, 1755), which is widespread in the Mediterranean ecosystem. This paper presents results of research on reproductive biology of Penaeus kerathurus in the Sea of Marmara. A total of 1404 specimens were dissected to determine the maturity stages, reproduction time, and length at first reproduction. The female-to-male ratio was 1:0.9. A high percentage of mature females was determined in April, May and July. Moreover, the value of the gonadosomatic index (GSI) decreased after July. The spawning period was observed between April and July, and its intensity increased in July. The carapace length at first sexual maturity was determined to be 39 mm.

scholarly journals Gametogenesis in Pubescent Heterobranchus longifilis (Teleostei: Clariidae) (Val. 1840) Attaining Puberty Under Artificial Conditions; and Under Rainy and Dry Season Conditions, in Tropical Earthen Ponds

2005 ◽  
Vol 18 (2) ◽  
Author(s):  
C.O. OFOR
Keyword(s):  
Rainy Season ◽  
Sexual Maturity ◽  
Gonadosomatic Index ◽  
Dry Season ◽  
Ovarian Maturation ◽  
Earthen Ponds ◽  
Group 3 ◽  
Group 2 ◽  
Heterobranchus Longifilis ◽  
Group 1

Three groups of Heterobranchus longifilis attained puberty under three environmental conditions. In experiment 1, 23 fish (10 males, 13 females) were raised in the rainy season, (group 1). In experiment 2, 90 fish (39 males, 51 females) were raised in a recirculation system (group 2). In experiment 3, 160 fish (73 males, 87 females) were raised in the dry season (group 3 ). Groups 1 and 3 were raised in outdoor earthen ponds. Gametogenesis in the groups was studied using histology and gonadosomatic index. Oocytes maturation commenced at 9½ months in all groups and lasted 67 days in group 1, and about 130 days in groups 2 and 3. Sexual maturity occurred at 352, 427, and 425 days respectively in groups 1, 2 and 3. It seems that the key point in ovarian maturation in pubescent Heterobranchus longifilis is 9½ months, with further oogenesis being influenced by rainfall. The time spent from here to sexual maturity onset may be shortened if it falls within the rainy season. The period may run its full course if it falls within the dry season, or the rearing environment is indoor. The application of these observations in the aquaculture of the species is discussed.

Embryo development capacity of oocytes fertilized by immature sperm and sperm treated with motility stimulants

1994 ◽  
Vol 6 (1) ◽  
pp. 113 ◽  
Author(s):  
O Lacham-Kaplan ◽  
AO Trounson
Keyword(s):  
Embryonic Development ◽  
Embryo Development ◽  
Sperm Motility ◽  
Cumulus Cells ◽  
Progressive Motility ◽  
Development Capacity ◽  
Fertilizing Ability ◽  
Embryonic Arrest ◽  
Human And Mouse

The fertilizing ability of mouse spermatozoa develops during maturation and coincides with the acquisition of motility. The lack of progressive motility of spermatozoa from the testis and precaudal segments of the epididymis interferes with their ability to fertilize oocytes after insemination in vitro. The removal of cumulus cells for insemination in vitro and the use of subzonal injection of a single spermatozoon resulted in a higher number of oocytes fertilized by immature caput and corpus spermatozoa. High rates of embryonic arrest and retarded development were observed in oocytes fertilized by caput and corpus spermatozoa when compared with oocytes fertilized by cauda spermatozoa. However, when the oocytes were enclosed in their cumulus cells or microinjected with a single spermatozoon, these effects were reduced. A block in embryonic development was also observed after human and mouse oocytes were exposed to the sperm motility stimulants pentoxifylline (PTF) and 2-deoxyadenosine (DOA). These observations suggest that exposure of oocytes to PTF and DOA should be avoided.

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