Targeted molecular mapping of a major wheat QTL for Fusarium head blight resistance using wheat ESTs and synteny with rice

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 817-823 ◽  
Author(s):  
Sixin Liu ◽  
James A Anderson
Keyword(s):  
Fusarium Head Blight ◽  
Molecular Mapping ◽  
Fusarium Head Blight Resistance ◽  
Rice Chromosome ◽  
Wheat Chromosome ◽  
Artificial Chromosome ◽  
Sequence Information ◽  
Head Blight ◽  
Wheat Ests ◽  
Sts Markers

A major QTL for resistance to Fusarium head blight (FHB) in wheat, Qfhs.ndsu-3BS, has been identified and verified by several research groups. The objective of this study was to increase the marker density in this QTL region using STS (sequence-tagged site) markers developed from wheat expressed sequence tags (ESTs) near Qfhs.ndsu-3BS. Because wheat chromosome 3BS and rice chromosome 1S are syntenous, the sequences of P1-derived artificial chromosome (PAC) and (or) bacterial artificial chromosome (BAC) clones covering the sub-distal portion of rice chromosome 1S were used as queries for a BLASTn search to identify wheat ESTs most likely near Qfhs.ndsu-3BS. Sixty-eight out of 79 STS primer pairs designed from wheat ESTs amplified PCR products from the genomic DNA of Triticum aestivum 'Chinese Spring'. Twenty-eight STS markers were localized on chromosome 3BS by aneuploid analysis. Six out of the nine STS markers that could be mapped in the T. aestivum 'Sumai 3'/T. aestivum 'Stoa' population had higher R2 and LOD values for this QTL than the most significant marker reported previously. Therefore, leveraging genome sequence information available in rice for wheat genetics is an effective strategy to develop DNA markers for Qfhs.ndsu-3BS, and this strategy may have broad applications for targeted mapping of other traits in cereal crops.Key words: comparative mapping, genomics.

Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a ‘Wangshuibai’ derived population

2008 ◽  
Vol 35 (10) ◽  
pp. 625-629 ◽  
Author(s):  
Amir Mohammad Naji ◽  
Mohammad Moghaddam ◽  
Mohammad Reza Ghaffari ◽  
Hashem Pour Irandoost ◽  
Laleh Karimi Farsad ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Head Blight ◽  
Sts Markers

scholarly journals A spontaneous segmental deletion from chromosome arm 3DL enhances Fusarium head blight resistance in wheat

Genome ◽  
2015 ◽  
Vol 58 (11) ◽  
pp. 479-488 ◽  
Author(s):  
David F. Garvin ◽  
Hedera Porter ◽  
Zachary J. Blankenheim ◽  
Shiaoman Chao ◽  
Ruth Dill-Macky
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Fusarium Head Blight Resistance ◽  
Disease Spread ◽  
Resistance Locus ◽  
Head Blight ◽  
Chromosome Arm ◽  
Fhb Resistance

Much effort has been directed at identifying sources of resistance to Fusarium head blight (FHB) in wheat. We sought to identify molecular markers for what we hypothesized was a new major FHB resistance locus originating from the wheat cultivar ‘Freedom’ and introgressed into the susceptible wheat cultivar ‘USU-Apogee’. An F2:3 mapping population from a cross between Apogee and A30, its BC4 near-isoline exhibiting improved FHB resistance, was evaluated for resistance. The distribution of FHB resistance in the population approximated a 1:3 moderately resistant : moderately susceptible + susceptible ratio. Separate disease evaluations established that A30 accumulated less deoxynivalenol and yielded a greater proportion of sound grain than Apogee. Molecular mapping revealed that the FHB resistance of A30 is associated with molecular markers on chromosome arm 3DL that exhibit a null phenotype in A30 but are present in both Apogee and Freedom, indicating a spontaneous deletion occurred during the development of A30. Aneuploid analysis revealed that the size of the deleted segment is approximately 19% of the arm’s length. Our results suggest that the deleted interval of chromosome arm 3DL in Apogee may harbor FHB susceptibility genes that promote disease spread in infected spikes, and that their elimination increases FHB resistance in a novel manner.

Transfer of Fusarium Head Blight Resistance from Thinopyrum elongatum to bread wheat cultivar Chinese Spring

Genome ◽  
2021 ◽  
Author(s):  
George Fedak ◽  
Dawn Chi ◽  
Danielle Wolfe ◽  
Thérèse Ouellet ◽  
Wenguang Cao ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Bread Wheat ◽  
Chinese Spring ◽  
Wheat Cultivar ◽  
Fusarium Head Blight Resistance ◽  
Wheat Chromosome ◽  
Head Blight ◽  
Genome Chromosome ◽  
Bread Wheat Cultivar ◽  
Thinopyrum Elongatum

The diploid form of Tall Wheatgrass, Thinopyrum elongatum (Host) D. R. Dewey (2n = 2x = 14, EE genome) has a high level of resistance to Fusarium head blight. The symptoms do not spread beyond the inoculated floret following point inoculation. Using the series of E genome chromosome additions in a bread wheat cultivar Chinese Spring (CS) background, the resistance was found to be localized to the long arm of chromosome 7E. CS mutant ph1b was used to induce recombination between chromosome 7E, present in the 7E(7D) substitution and homoeologous wheat chromosomes. Multivalent chromosome associations were detected in BC1 hybrids attesting to the effectiveness of the ph1b mutant. Genetic markers specific for chromosome 7E were used to estimate the size of the 7E introgression in the wheat genome. Using single sequence repeat (SSR) markers specific for homoeologous wheat chromosome 7, introgressions were detected on wheat chromosomes 7A, 7B and 7D. Some of the introgression lines were resistant to Fusarium head blight.

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