Microsatellite variability and heterozygote deficiency in the arctic–alpine Alaskan wheatgrass (Elymus alaskanus) complex

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 729-737 ◽  
Author(s):  
Genlou Sun ◽  
Björn Salomon

Genetic variation in the allotetraploid grass Elymus alaskanus complex was assessed using microsatellites in seven populations from Canada, Greenland, and the U.S.A. Microsatellite variation was compared with allozyme and RAPD variation. Our results indicated that E. alaskanus was highly homozygous but also highly variable. The polymorphic loci ranged from 50 to 100% with a mean of 78.6%, and the mean number of allele per locus was 3.14. Average expected heterozygosity value (HE, gene diversity) varied across populations and ranged from 0.244 to 0.651 with mean of 0.414. The mean value of HE across Canadian populations (0.517) was significantly higher than that across populations in Greenland (0.367). The correlation between allozyme and microsatellite gene diversity value (HE) showed a high positive correlation (r = 0.68), but between RAPD and microsatellite showed a low positive correlation (r = 0.08). Populations were highly differentiated, with 38% of variation among populations. Interpopulation genetic distance showed no association with geographic distance between the population sites of origin. A Hardy-Weinberg exact test for all loci and all populations reveals a significant heterozygote deficiency. Possible explanations for heterozygote deficiency are discussed.Key words: Elymus alaskanus, microsatellites, heterozygote deficiency, genetic differentiation, variability.

Author(s):  
IRENGBAM SUSUPRIYA DEVI ◽  
GLADYS RAI ◽  
V. P. S PUNIA ◽  
MANOJ KUMAR NANDKEOLIAR

Objective: This study aimed to find the correlation between body mass index (BMI) and microalbuminuria (MA) in essential hypertensive adults. Methods: This study included 35 essential hypertensive patients in the 18–65 years of age group, who satisfied the inclusion criteria. Arterial blood pressure was measured by a sphygmomanometer and the first voided early morning urine sample was collected for the estimation of microalbumin. Results: The mean age of the population studied was 49±11.08 in essential hypertensive patients and 46.89±11.10 in the control group. The mean value of BMI in the hypertensive group was found to be 27.59±5.45. In the present study, the mean value of microalbumin in essential hypertensive patients was 20.95±16.96. A significant positive correlation between BMI and MA was observed in the study with p<0.001. Conclusion: Our study shows a positive correlation between BMI and MA. Therefore, this study will help in the early detection of renal injury and prevents its progression to renal failure by lifestyle and diet modifications.


1977 ◽  
Vol 85 (3) ◽  
pp. 636-643 ◽  
Author(s):  
H. Svan ◽  
K. Hall ◽  
M. Ritzén ◽  
K. Takano ◽  
A. Skottner

ABSTRACT Somatomedin A by radioreceptor assay and somatomedin B by radioimmunoassay were measured in serum from women immediately after parturition and from their newborns. The mean levels of somatomedin A in both mothers (0.54 U/ml) and infants (0.50 U/ml) were significantly decreased compared to a reference group consisting of 21 non-pregnant women (0.91 U/ml). There was no difference between the mothers and their children. The mean somatomedin B value in serum from the mothers (63.9 μg/ml) was above that found in non-pregnant women (19.2 μg/ml) and the mean value in cord blood and serum from the infants (7.1 μg/ml) was below it. A positive correlation was noted between somatomedin A and B in cord blood; r = 0.78. The combined somatomedin A and B values in cord blood were positively correlated to birth weight (r = 0.51, P < 0.05).


2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ayman Abd Al-Maksoud Yousef ◽  
Ghada Abdulmomen Suliman ◽  
Maaly Mohamed Mabrouk

Background. The clinical management of sepsis is a highly complicated process. Disruption of the immune system explains in part the major variation in sepsis outcome. IL-8 is a proinflammatory cytokine, genetic polymorphism of this cytokine could explain the outcome of sepsis. The present study was conducted to determine the value of serum IL-8 monitoring and its (-251A/T) genetic polymorphism in critically ill patients. Patients and Methods. 180 critically ill patients were allocated into two groups, 90 septic patients (sepsis group) and 90 nonseptic patients (SIRS group). Admission serum IL-8 and its (-251A/T) mutant allele were detected. Results. The admission mean value of serum IL-8 was significantly elevated in sepsis group. In both groups, the mean value of serum IL-8 in nonsurvived patients and patients with IL-8 (-251A/T) mutant allele was significantly higher. A positive correlation of survival and IL-8 (-251A/T) mutant allele was detected in both groups. The serum IL-8 distinguished wild from IL-8 (-251A/T) mutant allele at a cut-off value of 600 pg/mL. Conclusion. The admission mean value of serum IL-8 was significantly elevated in septic, nonsurvived, and patients with IL-8 (-251A/T) mutant alleles. A positive correlation of survival and IL-8 (-251A/T) mutant allele patients was detected.


2019 ◽  
Vol 7 (1) ◽  
pp. 53-55 ◽  
Author(s):  
Imam Budi Putra ◽  
Remenda Siregar ◽  
Nelva K. Jusuf ◽  
Oratna Ginting ◽  
Raja Nurhayati

BACKGROUND: Skin tag is a benign tumour of connective tissue in the skin, sessile or pedunculated, skin-like to brownish coloured and often arises in the flexure area. Etiopathogenesis of skin tag is still unclear, but one of the aetiology is associated with leptin hormone. AIM: To determine the correlation between leptin serum level with type and number of the lesion skin tag. METHODS: This study is an observational analytic study with a cross-sectional design involving 33 skin tag patients. Diagnosis of skin tag was based on history and clinical examination; we conducted blood sampling and measurement of serum leptin level to the patients. RESULTS: We found the mean serum leptin level of skin tag patients were highest on the type of lesion mixed (31.54 ± 12.85 ng/ml). The mean number of skin tag lesions was 13.6 ± 5.8 lesions. There is a very high positive correlation between serum leptin level with a number of skin tag (r = 0.86) with p < 0.05 and significant correlation between serum leptin level with the type of lesions (p = 0.037). CONCLUSION: Serum leptin level has a very high positive correlation to a number of skin tag and significant correlation between serum leptin level with the type of lesion.


In October 1976 a cooperative experiment was made to survey the sea-ice topography in the European sector of the Arctic Ocean. H. M. submarine Sovereign acquired 4000 km of ice draft data by using an upward-looking sonar, while a Canadian Forces aircraft flew along the submarine’s track and acquired 2200 km of ice elevation data by using a laser profilometer. The two types of profile were processed in corresponding 100 km section lengths, and the following statistical analyses and comparisons were made: (i) Probability density functions o f ice draft and elevation. Each distribution shows a peak for young ice and for undeformed multi-year ice. At large ice thicknesses the distributions take the analytical form of a negative exponential. The mean drafts enable two distinct geographical ice regimes to be identified. There is an ‘offshore zone’ of very heavy pressure ridging extending up to 400 km from the coasts of Ellesmere Island and of north Greenland, with mean ice draft in the range 5.0 to 7.5 m , while out in the central Arctic Ocean the mean ice draft is lower (3.9—5.1 m) and the characteristics of the ice cover remain homogeneous over a length scale of 1000 km. The transition between the two regimes is abrupt, taking place in less than 25 km. Data from the same part of the central Arctic taken in March 1971 showed a mean ice draft 0.3 m lower, while data from the central Beaufort Sea showed a mean draft more than 0.8 m lower. (ii) Level ice distributions. Ice with a local gradient of less than 1 in 40 was defined as level ice, and used as an indicator of the quantity and thickness distribution of undeformed (i.e. thermodynamically grown) ice in the Arctic Ocean. The distribution has a mode at 3.0—3.1 m draft, and level-ice percentages are in the range 30—40 (bottom side) and 70—80 (top side) in the offshore zone, and 45—55 (bottom) and 85-95 (top) in the central Arctic. Thus about half of the Arctic ice cover consists of deformed ice. (iii) Pressure ridge spacings. The spacings of ridge keels fit a negative exponential distribution, characteristic of randomness, except at close spacings where there is a deficit of keels (explained as a geometrical effect) and at very large spacings where there is an excess (due to the contribution of polynyas). The distribution of sail spacings exhibits these two effects, but also differs from a random distribution at moderate sail separations. (iv) Ridge elevations and drafts. Keel drafts fit a law of form P( h ) d oc exp ( — Ah 2 ) d h , except for an excess of keels at drafts beyond 20 m. There is a positive correlation between mean keel draft and keel frequency. There are 3.5—4.5 keels per kilometre with draft exceeding 9 m in the offshore zone and 2—3 in the central Arctic. Sail elevations fit a law of form P( h ) d h x exp ( — A h ) d h , with a positive correlation between mean sail elevation and sail frequency. The sail elevation and ice elevation distributions can be related by assuming that all thick ice is contained in pressure ridges of triangular cross section. (v) Keel—sail comparison. For the 21 corresponding 100 km sections there are positive correlations between mean sail height and mean keel draft, and between keel frequency and sail frequency. From these it is possible to convert a sail distribution (computed from a laser profile) into a keel distribution, enabling sea ice bulk characteristics to be derived from airborne surveys alone. (vi) Leads and polynyas . A lead was defined as a continuous sequence of depth points greater than 5 m long and not exceeding 1 m draft. The number density n ( d ) of leads per kilometre of width d m fits the power law n ( d ) — 15 d -2 . Exceptionally wide leads were concentrated in the offshore zone and in the marginal ice zone close to the open water of the Greenland Sea.


Author(s):  
R. C. Mossman

The average variability of temperature at any place is obtained by taking the difference of temperature at the same hour on successive days, and taking the mean value of this difference irrespective of sign. With a view to ascertaining whether this variability varied with the hour selected for comparison, the hourly temperature records at four places have been examined, and the day to day change of temperature at each hour noted. The places are—the Ben Nevis Observatory, the Fort-William Observatory, the Hong Kong Observatory, and the Arctic Station at Lady Franklin Bay, lat. 81° 44′ N., long. 64° 45′ W. At each place one year's record was taken, which, though too short a time to give a true mean, yet gives a fair approximation towards it. The mean values for each hour of the twelve months at the four stations are given in the accompanying tables, the highest value in each month being put in heavy type and the lowest in italic.


2016 ◽  
Vol 8 (9) ◽  
pp. 11
Author(s):  
Raed Jameel Jaber ◽  
Marwan Mohammad Abu Fadda

<p>This research paper aimed at determining Awareness Level of Professional Independence Requirements (ALOPIR), through assimilation of fundamental principles of professional ethics, by Jordanian CPA auditors, in auditing process. A questionnaire was established and developed for this purpose and given out to external Jordanian audit firms and CPA auditors, the number of questionnaires which were exposed to analysis was (40), extracted data was analyzed using (SPSS), and a number of statistical tests; was achieved.</p><p>The study found positive correlation relationships between the stated variables; (high positive correlation between “professional competence and due care, professional behavior, safeguards threats”; moderate positive correlation between “objectivity, confidentiality”; low positive correlation between “integrity” and (ALOPIR)),</p><p>Despite the fact that the results indicate to the existence of certain (ALOPIR) by Jordanian CPA auditors; yet the low and moderate correlation of some variables may emerge a sign that the auditors awareness of some independence concepts is still below the demanded level; as the correlation was low with integrity and moderate with objectivity and confidentiality, such a matter must be paid more attention. The low positive correlation between integrity and<strong> </strong>(ALOPIR) can be explained by the probable that auditors may predominant their financial interests than to achieve integrity and honesty; this may be due to the absence of a declared Ethics Charter of audit in Jordan.</p><p>A standardized mean test was done to accept or reject either the null hypothesis or the alternative hypothesis, according to a comparison between each of the variables’ joint mean and the standardized one, following that it was possible to descending sorting of the hypotheses depending on the joint mean value.</p>A main recommendation was introduced which is: to take steps to achieve imminent, deep and effectively spreading of relative culture, especially the fundamental principles of professional ethics, among professional accountants.


Author(s):  
C. Cheng

1. The number of species found in this investigation is six, of which four are marine and two brackish water.2. The mean value of the body weight and the fecundity varies with the species. The size of a species bears no relation whatever to the reproductive capacity.3. The fecundity is subject to seasonal variation, being generally higher in the spring than in the winter months.4. In general, a positive correlation exists between body weight and fecundity of the individuals of each species. This rule does not, however, apply to Gammarus duebeni, in which the fecundity appears to fall after reaching its maximum at the 19 mg. body-weight class.


2021 ◽  
Vol 3 (3) ◽  
pp. 230-241
Author(s):  
C. M. Senanayake

Background: Nurses are an essential component of health workforce. Therefore rewarding nurses is important to provide better service to the clients. The main purpose was to describe the effect of rewards on the work commitment of nurses at the SMS of National hospital. Objectives: to identify intrinsic and extrinsic rewards cause to the level of work commitment of nurses at SMS of National hospital, to determine the level of work commitment of nurses at SMS of National hospital, to measure the effect of socio-demographic factors on work commitment of nurses at SMS of National hospital and to describe the effect of rewards on work commitment of nurses at SMS of National hospital. Methodology: Descriptive, hospital-based, cross-sectional study design was used. Simple random sampling used to select 384 nurses. Data was collected using a self-administered questionnaire. Results: The mean value for composite intrinsic rewards was 3.34 (SD = 0.57). There is a moderately positive Correlation (r = 0.327, p< 0.000) between intrinsic rewards and the work commitment. The mean value for composite extrinsic rewards was 2.54 (SD = 0.71) and it shows a very weak positive Correlation (r = 0.167, p< 0.002) between extrinsic rewards and work commitment. As Socio-demographic factors, gender (0.016**), age group (0.117*), position (0.114*), working experience at current work place (0.154*), and working section (0.014**) have association with the overall work commitment of nurses. Level of work commitment of nurses was moderate (Mean = 3.13, SD = 0.35). Conclusion: Moderate correlation means, when increases intrinsic rewards, nurses’ work commitment can be increased. Extrinsic rewards also cause to increase the commitment. Socio-demographic factors have an association with overall work commitment. These results help nurse managers to improve existing intrinsic rewards for nurses to enhance work commitment. Nurse Managers should suggest and plan a good reward system for nurses. Further research wants to conduct in government and private hospitals to find out ways to make nurses more committed. Moderate work commitment may be due to inadequate rewards or actually their dedication to the service without expecting rewards. Doi: 10.28991/SciMedJ-2021-0303-4 Full Text: PDF


2021 ◽  
Vol 66 (No. 1) ◽  
pp. 1-12
Author(s):  
Lenka Štohlová Putnová ◽  
Radek Štohl

The paper demonstrates the dependability of assignment testing in the identification of an appropriate breed to monitor comprehensive genetic information from molecular markers to analyse the collection of real population data covering 22 horse breeds registered in the Czech Republic, including native breeds and genetic resources. If 17 microsatellites are used, the mean number of alleles per locus corresponds to 10.4. The count of alleles at the individual loci ranges between five (HTG07) and 17 (ASB17). The loci ASB02, ASB23, HMS03, HTG10, and VHL20 exhibit the highest gene diversity and observed heterozygosity (both above 80%), with the mean value of 0.77 and 0.73, respectively. The moderate total inbreeding coefficient (5.2%) is estimated across all the loci and breeds. The levels of apparent breed differentiation span from zero between the Czech Warmblood and Slovak Warmblood to 0.15 between the Shetland Pony and Standardbred. The phylogenetic breed relationships are revealed via the NeighbourNet dendrogram constructed from Reynolds’ genetic distances, which clearly separate the Coldblood draught, Hot/Warmblood, and Pony horses. Our results reveal that the Bayesian approach (the Rannala and Mountain technique) provides the most intensive prediction power (83.6%) out of the GeneClass tools and that the Bayes Net algorithm exhibits the best efficiency (78.4%) from the WEKA machine learning workbench options, considering the use of the five-fold cross validation technique. The algorithms could be trained on large real reference data sets, and thus there appears another viable perspective for machine learning in horse ancestry testing. In this context, it is also important to stress the fact that innovated computational tools will potentially lead towards structuring a novel web server to allow the identification of horse breeds.


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