A combined RFLP–SSR–AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 612-626 ◽  
Author(s):  
J -M Lacape ◽  
T -B Nguyen ◽  
S Thibivilliers ◽  
B Bojinov ◽  
B Courtois ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Quantitative Traits ◽  
Gossypium Barbadense ◽  
Tetraploid Cotton ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Dense Region ◽  
Backcross Population ◽  
Integrated Map ◽  
Chromosome Assignment

An interspecific Gossypium hirsutum × Gossypium barbadense backcross population of 75 BC1 plants was evaluated for 1014 markers. The map consists of 888 loci, including 465 AFLPs, 229 SSRs, 192 RFLPs, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altogether spanning 4400 cM. Loci were not evenly distributed over linkage groups, and 18 of the 26 long groups had a single dense region. This paper proposes a partially revised list of the 13 pairs of homoeologous A/D chromosomes of the 2n = 4x = 52 tetraploid cotton genome. The major revisions, which involve the c3–c17, c4–c22, c5–D08, and c10–c20 homoeologous pairs, are based on the mapping of 68 SSR and RFLP loci with a known chromosome assignment, as well as on comparative alignments with previously published G. hirsutum × G. barbadense maps. The overall congruency in the locus orders and distances of common SSR and RFLP loci in these maps allows for an estimation of the consensus length that reaches a minimum of 5500 cM, and is encouraging for future efforts aimed at developing an integrated map of cultivated cotton. The present map also provides a firm framework for precision mapping of Mendelian components of quantitative traits in cotton.Key words: cotton, genetic mapping, RFLP, microsatellite, AFLP.

A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L.

Genome ◽  
2005 ◽  
Vol 48 (3) ◽  
pp. 378-390 ◽  
Author(s):  
Xianliang Song ◽  
Kai Wang ◽  
Wangzhen Guo ◽  
Jun Zhang ◽  
Tianzhen Zhang
Keyword(s):  
Gossypium Hirsutum ◽  
Average Distance ◽  
Gossypium Barbadense ◽  
Genetic Maps ◽  
Marker Genes ◽  
Tetraploid Cotton ◽  
Linkage Groups ◽  
Gossypium Hirsutum L ◽  
Morphological Marker ◽  
Map Comparison

Simple sequence repeat (SSR) genetic maps have been separately constructed based on doubled haploid (DH) and (or) haploid and BC1 populations from the same cross between Gossypium hirsutum L. 'TM-1' and Gossypium barbadense L. 'Hai7124'. The BC1 population was produced by pollinating individual plants of the 'TM-1' × 'Hai7124' F1 with 'TM-1', whereas the DH and (or) haploid population developed from the offspring of Vsg × ('TM-1' × 'Hai7124'). Vsg is a virescently marked semigamy line of Gossypium barbadense L. Pima. The BC1 map included 34 linkage groups with an average distance between markers of 9.80 cM (Kosambi, K) and covered 4331.2 cM (K) or approximately 78.7% of the tetraploid cotton genome constructed using 440 SSR and 2 morphological marker genes. Among them, 26 were assigned to 20 chromosomes, 7 to A or D subgenomes, and 1 was unassigned. The haploid map comprised 444 SSR markers mapped to 40 linkage groups with an average distance of 7.35 cM (K) between markers, covering 3262.9 cM (K) or approximately 60.0% of the tetraploid genome. Twenty-nine linkage groups were assigned to all 19 identified chromosomes, 10 to A or D subgenomes, and 1 was unassigned. Fairly good collinearity of marker order was observed along most of the chromosomes or linkage groups. Significant differences in recombination between maps was observed at the chromosomal and genomic level and possible reasons were discussed. Map comparison and combined data provided an essential basis for further mapping of interested genes and QTLs and for studies of diversity, population structure, and phylogeny in Gossypium species.Key words: cotton, SSR, comparative mapping, semigamy.

scholarly journals A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.

Genetics ◽  
1994 ◽  
Vol 138 (3) ◽  
pp. 829-847 ◽  
Author(s):  
A J Reinisch ◽  
J M Dong ◽  
C L Brubaker ◽  
D M Stelly ◽  
J F Wendel ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Nuclear Dna ◽  
Fragment Length Polymorphism ◽  
Natural Fiber ◽  
Gossypium Barbadense ◽  
Chromosome Organization ◽  
Linkage Groups ◽  
Rflp Map ◽  
Polyploid Genome ◽  
Polyploidization Event

Abstract We employ a detailed restriction fragment length polymorphism (RFLP) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear DNA probes detect RFLPs distinguishing Gossypium hirsutum and Gossypium barbadense; and 705 RFLP loci are assembled into 41 linkage groups and 4675 cM. The subgenomic origin (A vs. D) of most, and chromosomal identity of 14 (of 26), linkage groups is shown. The A and D subgenomes show similar recombinational length, suggesting that repetitive DNA in the physically larger A subgenome is recombinationally inert. RFLPs are somewhat more abundant in the D subgenome. Linkage among duplicated RFLPs reveals 11 pairs of homoelogous chromosomal regions-two appear homosequential, most differ by inversions, and at least one differs by a translocation. Most homoeologies involve chromosomes from different subgenomes, putatively reflecting the n = 13 to n = 26 polyploidization event of 1.1-1.9 million years ago. Several observations suggest that another, earlier, polyploidization event spawned n = 13 cottons, at least 25 million years ago. The cotton genome contains about 400-kb DNA per cM, hence map-based gene cloning is feasible. The cotton map affords new opportunities to study chromosome evolution, and to exploit Gossypium genetic resources for improvement of the world's leading natural fiber.

scholarly journals Heterosis studies for quantitative traits in interspecific hybrids of cotton (Gossypium hirsutum L. × Gossypium barbadense L.)

2017 ◽  
Vol 9 ◽  
Author(s):  
Anil Devidas Adsare ◽  
Abhay Narayan Salve ◽  
Narendrakumar Prakash Patil
Keyword(s):  
Gossypium Hirsutum ◽  
Yield Components ◽  
Quantitative Traits ◽  
Large Scale ◽  
Gossypium Barbadense ◽  
Cotton Yield ◽  
Seed Cotton ◽  
Gossypium Hirsutum L ◽  
Seed Cotton Yield ◽  
Hybrid Development

<p>A study was conducted in interspecific cotton hybrids (<em>Gossypium hirsutum</em> L. × <em>Gossypium barbadense</em> L.) to assess the extent of the heterosis for eight yield and yield attributing traits. Twelve lines of <em>G. hirsutum</em> and five testers of <em>G. barbadense</em> were crossed in line x tester manner to develop 60 F<sub>1</sub> hybrids. Analysis of variance revealed the significant differences among the crosses for all traits. Heterosis was worked out over mid parent, better parent and two standard checks i.e. MRC 7918 and Varalaxmi. Crosses RAH1065 x SUJALA, PH1075 x SUJALA, PH348 x SUJALA and DHY286 x Suvin were the best heterotic combinations for seed cotton yield and could be utilized for hybrid development. These hybrids were shown desirable heterosis for seed cotton yield along with other yield components. These hybrids may be tested in large scale trial to confirm the superiority in heterosis.</p>

Gene Flow between Gossypium hirsutum L. and Gossypium barbadense L. is Asymmetric

Crop Science ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 298-305 ◽  
Author(s):  
Allen E. Van Deynze ◽  
Robert B. Hutmacher ◽  
Kent J. Bradford
Keyword(s):  
Gene Flow ◽  
Gossypium Hirsutum ◽  
Gossypium Barbadense ◽  
Gossypium Hirsutum L

Morphological and molecular characterization of teosinte derived maize population

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Amarjeet Kumar ◽  
N. K. Singh ◽  
Sneha Adhikari ◽  
Anjali Joshi
Keyword(s):  
Molecular Characterization ◽  
Quantitative Traits ◽  
Morphological Characterization ◽  
Linkage Groups ◽  
Germplasm Enhancement ◽  
Maize Germplasm ◽  
Maize Population ◽  
Quantitative Characters ◽  
Morphological And Molecular Characterization

Germplasm enhancement seems to be an essential part of a breeding programme to improve resiliency, adaptability and productivity of the crops. To develop and diversify the maize germplasm, teosinte a wild relative of maize was integrated in crossing programme and BC1F4 lines were developed. Five BC1F4 lines along with teosinte and maize inbred DI-103 were characterised using quantitative characters and molecular markers. Morphological characterization was done with the help of visual parameters and quantitative traits and for molecular characterization fifty six SSR markers were used. SSR data were analysed with the help of software Mapmaker and twelve linkage groups were generated. Maximum allelic contributions from parent teosinte were found in the introgressed line AM-5 (53.4%) followed by AM-12 (48.9 %), whereas, least contribution of 34.1 % was found in AM-7. The maximum genetic distance among the introgressed lines was observed between AM-2 and AM-9 (0.75) followed by AM-2 and AM-7 (0.70), AM-7 and AM-9 (0.70). The maximum number of cob was found in AM-5 (5.00) followed by AM-2 (4.00). Grain yield per plant was found highest for AM-2 (100.00 g) followed by AM-12 (80.00 g), while, least value was observed for AM-7 (42.00 g). The results indicated differential parental contributions which leads to diversification in the progenies derived from diverse crosses in maize and further opined that such crosses seems to be essential for creating adapted germplasm to whom breeders are looking for.

scholarly journals GENETIC STUDIES OF SOME QUANTITATIVE TRAITS IN TWO COTTON CROSSES (Gossypium barbadense L.)

2008 ◽  
Vol 86 (4) ◽  
pp. 1463-1475
Author(s):  
MOHAMED A. ABOU EL-YAZIED ◽  
ABDEL-NASER M. R. ABD EL-BARY ◽  
MOHAMED A. M. ALLAM
Keyword(s):  
Quantitative Traits ◽  
Gossypium Barbadense ◽  
Genetic Studies
Sign in / Sign up

Export Citation Format

Share Document