Molecular phylogeny of tephritid fruit flies in the Bactrocera tau complex using the mitochondrial COI sequences

Genome ◽  
2003 ◽  
Vol 46 (1) ◽  
pp. 112-118 ◽  
Author(s):  
Wanwisa Jamnongluk ◽  
Visut Baimai ◽  
Pattamaporn Kittayapong
Keyword(s):  
Cytochrome Oxidase ◽  
Locusta Migratoria ◽  
Sequence Divergence ◽  
Variable Region ◽  
Distinct Group ◽  
Cytochrome Oxidase I ◽  
Bactrocera Dorsalis ◽  
Species Differentiation ◽  
Premating Isolation ◽  
Mitochondrial Coi

We compared sequences of the mitochondrial cytochrome oxidase I gene of eight species of the Bactrocera tau complex using Bactrocera dorsalis,Bactrocera pyrifoliae,Ceratitis capitata, Anopheles gambiae, and Locusta migratoria as outgroups. A 639-bp variable region was sequenced. The sequence divergence between species in the B. tau complex ranged from 0.06 to 28%, and up to 29% between the complex and its tephritid outgroups, B. dorsalis and C. capitata. According to the phylogenetic relationships, these members of the B. tau complex could be classified into four clades. Thus, species A and D form clades 1 and 3, respectively, while species C and I belong to clade 4. However, species B, E, and F form a distinct group, clade 2, and infested the fruits of non-cucurbit hosts. Host-plant shifts resulting in adaptive radiation and premating isolation among species might play an important role in species differentiation of the B. tau complex.Key words: Bactrocera, cytochrome oxidase I, species complex, mitochondrial DNA, phylogeny.

scholarly journals Taxonomy of the Crematogaster degeeri-species-assemblage in the Malagasy region (Hymenoptera: Formicidae)

2013 ◽  
Author(s):  
Bonnie B. Blaimer ◽  
Brian L. Fisher
Keyword(s):  
Cytochrome Oxidase ◽  
Gene Tree ◽  
Sequence Divergence ◽  
Cytochrome Oxidase I ◽  
Species Level ◽  
Morphological Data ◽  
Species Assemblage ◽  
Distribution Maps ◽  
Species Groups ◽  
Species Descriptions

We revise the species-level taxonomy of the Crematogaster (Crematogaster) degeeri-species-assemblage, a group of related ants occuring in Madagascar and the wider Malagasy region, and further provide an identification key to all species-groups of the genus Crematogaster in this region. Within the C. degeeri-assemblage, we recognize twelve species based upon morphological data from worker, queen and male ants, as well as genetic data from the barcode region of cytochrome oxidase I. Seven new species are described: Crematogaster alafara Blaimer sp. nov., C. bara Blaimer sp. nov., C. mafybe Blaimer sp. nov., C. maina Blaimer sp. nov., C. malahelo Blaimer sp. nov., C. masokely Blaimer sp. nov., C. ramamy Blaimer sp. nov. Crematogaster tricolor Gerstäcker, 1859 (stat. rev.) and C. dentata Dalla Torre, 1893 (stat. nov.) are raised to species level, and the following new synonymies are proposed: Crematogaster degeeri lunaris Santschi, 1928 as a synonym of C. degeeri Forel, 1886; Crematogaster sewelli improba Forel, 1907 and C. sewelli mauritiana Forel, 1907 as synonyms of C. dentata Dalla Torre, 1893, and C. pacifica Santschi, 1919 as a synonym of C. lobata Emery, 1895. Species descriptions, images, and distribution maps and identification keys based on worker ants, as well as on queen ants where available, are presented for all twelve species. In addition, we present a molecular gene tree for cytochrome oxidase I and summarize levels of sequence divergence within and between species of the C. degeeri-species-assemblage. Our findings are discussed in the light of previous work on Malagasy Crematogaster ants.

scholarly journals Morphological, Ecological, and Molecular Divergence of Conogethes pinicolalis from C. punctiferalis (Lepidoptera: Crambidae)

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 455
Author(s):  
Na Ra Jeong ◽  
Min Jee Kim ◽  
Sung-Soo Kim ◽  
Sei-Woong Choi ◽  
Iksoo Kim
Keyword(s):  
Phylogenetic Analysis ◽  
First Generation ◽  
Incomplete Lineage Sorting ◽  
Divergence Time ◽  
Sequence Divergence ◽  
Lineage Sorting ◽  
Mitochondrial Coi ◽  
Feeding Type ◽  
Pine Trees ◽  
Yellow Peach Moth

Conogethes pinicolalis has long been considered as a Pinaceae-feeding type of the yellow peach moth, C. punctiferalis, in Korea. In this study, the divergence of C. pinicolalis from the fruit-feeding moth C. punctiferalis was analyzed in terms of morphology, ecology, and genetics. C. pinicolalis differs from C. punctiferalis in several morphological features. Through field observation, we confirmed that pine trees are the host plants for the first generation of C. pinicolalis larvae, in contrast to fruit-feeding C. punctiferalis larvae. We successfully reared C. pinicolalis larvae to adults by providing them pine needles as a diet. From a genetic perspective, the sequences of mitochondrial COI of these two species substantially diverged by an average of 5.46%; moreover, phylogenetic analysis clearly assigned each species to an independent clade. On the other hand, nuclear EF1α showed a lower sequence divergence (2.10%) than COI. Overall, EF1α-based phylogenetic analysis confirmed each species as an independent clade, but a few haplotypes of EF1α indicated incomplete lineage sorting between these two species. In conclusion, our results demonstrate that C. pinicolalis is an independent species according to general taxonomic criteria; however, analysis of the EF1α sequence revealed a short divergence time.

scholarly journals Mitochondrial gene expression in small intestinal epithelial cells

1995 ◽  
Vol 308 (2) ◽  
pp. 665-671 ◽  
Author(s):  
T P Mayall ◽  
I Bjarnason ◽  
U Y Khoo ◽  
T J Peters ◽  
A J S Macpherson
Keyword(s):  
Epithelial Cells ◽  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Intestinal Epithelial Cells ◽  
Mitochondrial Gene ◽  
Cytochrome Oxidase I ◽  
Mrna Levels ◽  
Intestinal Epithelial ◽  
Mitochondrial Transcription Factor ◽  
Small Intestinal

Most mitochondrial genes are transcribed as a single large transcript from the heavy strand of mitochondrial DNA, and are subsequently processed into the proximal mitochondrial (mt) 12 S and 16 S rRNAs, and the more distal tRNAs and mRNAs. We have shown that in intestinal epithelial biopsies the steady-state levels of mt 12 S and 16 S rRNA are an order of magnitude greater than those of mt mRNAs. Fractionation of rat small intestinal epithelial cells on the basis of their maturity has shown that the greatest ratios of 12 S mt rRNA/cytochrome b mt mRNA or 12 S mt rRNA/cytochrome oxidase I mt mRNA are found in the surface mature enterocytes, with a progressive decrease towards the crypt immature enteroblasts. Cytochrome b and cytochrome oxidase I mt mRNA levels are relatively uniform along the crypt-villus axis, but fractionation experiments showed increased levels in the crypt base. The levels of human mitochondrial transcription factor A are also greater in immature crypt enteroblasts compared with mature villus enterocytes. These results show that the relative levels of mt rRNA and mRNA are distinctly regulated in intestinal epithelial cells according to the crypt-villus position and differentiation status of the cells, and that there are higher mt mRNA and mt TFA levels in the crypts, consistent with increased transcriptional activity during mitochondrial biogenesis in the immature enteroblasts.

scholarly journals Phylogenetic analysis of the grouper family (Serranidae) from various local markets in Indonesian waters using COI (cytochrome oxidase I)

2021 ◽  
Vol 6 (01) ◽  
pp. 74-82
Author(s):  
Tomi Apra Santosa ◽  
Winda Ayu Fietri ◽  
Abdul Razak ◽  
Ramadhan Sumarmin
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Cytochrome Oxidase ◽  
Descriptive Analysis ◽  
Cytochrome Oxidase I ◽  
Species Relationships ◽  
Literature Study ◽  
Similar Morphology ◽  
Qualitative Descriptive ◽  
Family Based

Grouper fish (Serranidae) is a type of fish found in Indonesian waters. However, not many people have conducted further research on phylogenetics based on COI (Cytochrome Oxidase I). This study aims to explain the phylogeny of grouper fish from the Serranidae family based on COI (Cytochrome Oxidase I). This research is a literature study. The research samples were grouper fish from Lombok, Karimunjawa, Lampung, Kendari, Madura, Tanakeke, and Numfor. The research instrument was a grouper-type observation sheet and an observation sheet for the results of the MEGA 7 application test. The data were analyzed using qualitative descriptive analysis. The results showed that the level of kinship between the species tested was very close, including Epinephelus areolatus, E. merra, E. fasciatus, E. longispinis, E. coioides, E. ongus, and E. coeruleopunctatus with all genetic distance averages type. 0.02. The conclusion of this study is that in general the species relationships found are several species found in the same location with similar morphology and diet.

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