scholarly journals Mapping quantitative trait loci in inbred backcross lines of Lycopersicon pimpinellifolium (LA1589)

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1189-1202 ◽  
Author(s):  
Sami Doganlar ◽  
Anne Frary ◽  
Hsin-Mei Ku ◽  
Steven D Tanksley
Keyword(s):  
Quantitative Trait Loci ◽  
Lycopersicon Esculentum ◽  
Quantitative Trait ◽  
Interspecific Cross ◽  
Tomato Production ◽  
Significant Qtls ◽  
Wild Allele ◽  
Inbred Backcross Lines ◽  
Trait Loci ◽  
Inbred Backcross

Although tomato has been the subject of extensive quantitative trait loci (QTLs) mapping experiments, most of this work has been conducted on transient populations (e.g., F2 or backcross) and few homozygous, permanent mapping populations are available. To help remedy this situation, we have developed a set of inbred backcross lines (IBLs) from the interspecific cross between Lycopersicon esculentum cv. E6203 and L. pimpinellifolium (LA1589). A total of 170 BC2F1 plants were selfed for five generations to create a set of homozygous BC2F6 lines by single-seed descent. These lines were then genotyped for 127 marker loci covering the entire tomato genome. These IBLs were evaluated for 22 quantitative traits. In all, 71 significant QTLs were identified, 15% (11/71) of which mapped to the same chromosomal positions as QTLs identified in earlier studies using the same cross. For 48% (34/71) of the detected QTLs, the wild allele was associated with improved agronomic performance. A number of new QTLs were identified including several of significant agronomic importance for tomato production: fruit shape, firmness, fruit color, scar size, seed and flower number, leaf curliness, plant growth, fertility, and flowering time. To improve the utility of the IBL population, a subset of 100 lines giving the most uniform genome coverage and map resolution was selected using a randomized greedy algorithm as implemented in the software package MapPop (http://www.bio.unc.edu/faculty/vision/lab/mappop/). The map, phenotypic data, and seeds for the IBL population are publicly available (http://soldb.cit.cornell.edu) and will provide tomato geneticists and breeders with a genetic resource for mapping, gene discovery, and breeding.Key words: tomato, Lycopersicon esculentum, IBLs, QTL, mapping.

scholarly journals Linkage Analysis of Molecular Markers and Quantitative Trait Loci in Populations of Inbred Backcross Lines of Brassica napus L.

Genetics ◽  
1999 ◽  
Vol 153 (2) ◽  
pp. 949-964 ◽  
Author(s):  
David V Butruille ◽  
Raymond P Guries ◽  
Thomas C Osborn
Keyword(s):  
Genetic Variation ◽  
Brassica Napus ◽  
Quantitative Trait Loci ◽  
Qtl Analysis ◽  
Quantitative Trait ◽  
Agronomic Traits ◽  
Pedigree Structure ◽  
Inbred Backcross Lines ◽  
Trait Loci ◽  
Inbred Backcross

Abstract Backcross populations are often used to study quantitative trait loci (QTL) after they are initially discovered in balanced populations, such as F2, BC1, or recombinant inbreds. While the latter are more powerful for mapping marker loci, the former have the reduced background genetic variation necessary for more precise estimation of QTL effects. Many populations of inbred backcross lines (IBLs) have been developed in plant and animal systems to permit simultaneous study and dissection of quantitative genetic variation introgressed from one source to another. Such populations have a genetic structure that can be used for linkage estimation and discovery of QTL. In this study, four populations of IBLs of oilseed Brassica napus were developed and analyzed to map genomic regions from the donor parent (a winter-type cultivar) that affect agronomic traits in spring-type inbreds and hybrids. Restriction fragment length polymorphisms (RFLPs) identified among the IBLs were used to calculate two-point recombination fractions and LOD scores through grid searches. This information allowed the enrichment of a composite genetic map of B. napus with 72 new RFLP loci. The selfed and hybrid progenies of the IBLs were evaluated during two growing seasons for several agronomic traits. Both pedigree structure and map information were incorporated into the QTL analysis by using a regression approach. The number of QTL detected for each trait and the number of effective factors calculated by using biometrical methods were of similar magnitude. Populations of IBLs were shown to be valuable for both marker mapping and QTL analysis.

scholarly journals Mapping quantitative trait loci in Gallus gallus using principal components

2010 ◽  
Vol 39 (11) ◽  
pp. 2434-2441 ◽  
Author(s):  
Luís Fernando Batista Pinto ◽  
Irineu Umberto Packer ◽  
Mônica Corrêa Ledur ◽  
Ana Silvia Alves Meira Tavares Moura ◽  
Kátia Nones ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Principal Components ◽  
Quantitative Trait ◽  
Live Weight ◽  
Gallus Gallus ◽  
Heart Weight ◽  
Ratio Test ◽  
Linear Combinations ◽  
Significant Qtls ◽  
Trait Loci

This study aimed at mapping QTL (quantitative trait loci) using linear combinations of characteristics of economical interest in Gallus gallus. A total of 350 F2 chickens from an initial crossing among males from a broiler line (TT) with females from a layer line (CC) were used. It was conducted a QTL mapping in chromosomes of Gallus gallus (GGA1, GGA3, GGA5, GGA8, GGA11, and GGA13) for 20 performance and carcass traits. For detecting QTL, it was used the likelihood ratio test between a reduced model (including fixed effects of sex, hatch and random effect of infinitesimal genetic value) and a full model (including all the previous effects plus QTL effects). When original characterists were analyzed, that is, before the formation of linear combinations, six significant QTLs were mapped at 1% in the genome, four in the GGA1 (live weight at 35 days of age and at 42 days of age, abdominal fat and heart weight); and two on GGA3 (live weight at 35 and 42 days of age); three significant QTLs at 5% in the genome, one on GGA1 (head weight), one on GGA3 (wings weight), and one on GGA8 (gizzard weight); besides seven suggestive linkages for several traits. When QTLs were mapped for principal components, many mapped QTLs were confirmed for original traits, in addition to finding three QTLs and eight suggestive linkages not mapped for the original traits.

Mapping Quantitative Trait Loci for Partial Resistance to Phytophthora sojae in a Soybean Interspecific Cross

Crop Science ◽  
2010 ◽  
Vol 50 (2) ◽  
pp. 628-635 ◽  
Author(s):  
D. M. Tucker ◽  
M. A. Saghai Maroof ◽  
S. Mideros ◽  
J. A. Skoneczka ◽  
D. A. Nabati ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Partial Resistance ◽  
Interspecific Cross ◽  
Phytophthora Sojae ◽  
Trait Loci

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max ) and ‘PI 366121’ (Glycine soja )

2017 ◽  
Vol 136 (3) ◽  
pp. 379-385 ◽  
Author(s):  
Sanjeev K. Dhungana ◽  
Krishnanand P. Kulkarni ◽  
Cheol W. Park ◽  
Hyun Jo ◽  
Jong T. Song ◽  
...  
Keyword(s):  
Glycine Max ◽  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Glycine Soja ◽  
Starch Content ◽  
Interspecific Cross ◽  
Soybean Seed ◽  
Trait Loci

scholarly journals Quantitative Trait Loci Mapping of Multiple Independent Loci for Resistance to Fusarium oxysporum f. sp. vasinfectum Races 1 and 4 in an Interspecific Cotton Population

2018 ◽  
Vol 108 (6) ◽  
pp. 759-767 ◽  
Author(s):  
Congli Wang ◽  
Mauricio Ulloa ◽  
Tra Duong ◽  
Philip A. Roberts
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Oxysporum ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Interspecific Cross ◽  
Minor Effect ◽  
Root Knot Nematode ◽  
Major Qtls ◽  
Trait Loci ◽  
Plant Injury

Fusarium wilt, caused by the soilborne fungal pathogen Fusarium oxysporum f. sp. vasinfectum, is a vascular disease of cotton (Gossypium spp.). F. oxysporum f. sp. vasinfectum race 1 (FOV1) causes major plant injury and yield loss in G. hirsutum cultivars with coinfection with root-knot nematode (Meloidogyne incognita), while F. oxysporum f. sp. vasinfectum race 4 (FOV4) causes plant damage without nematode coinfection in G. hirsutum and in G. barbadense cultivars. Quantitative trait loci (QTL) analysis of the interspecific cross G. barbadense Pima S-7 × G. hirsutum Acala NemX revealed separate multiple loci determining resistance to FOV1 and FOV4, confirming that race specificity occurs in F. oxysporum f. sp. vasinfectum. Based on the area under the disease progress stairs, six major QTLs on chromosomes (Chrs) 1, 2, 12, 15 (2), and 21 contributing 7 to 15% to FOV1 resistance and two major QTLs on Chrs 14 and 17 contributing 12 to 33% to FOV4 resistance were identified. Minor-effect QTLs contributing to resistance to both FOV1 and FOV4 were also identified. These results define and establish a pathosystem of race-specific resistance under polygenic control. This research also validates the importance of previously reported markers and chromosome regions and adds new information for the location of F. oxysporum f. sp. vasinfectum resistance genes. Some F8 recombinant inbred lines have resistance to both FOV1 and FOV4 and also to root-knot nematode, providing multiple resistance sources for breeding.

Comparison of salt tolerance during seed germination and vegetative growth in tomato by QTL mapping

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 727-734 ◽  
Author(s):  
M R Foolad
Keyword(s):  
Quantitative Trait Loci ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Lycopersicon Esculentum ◽  
Quantitative Trait ◽  
Vegetative Growth ◽  
Developmental Stages ◽  
Phenotypic Correlation ◽  
Recurrent Parent ◽  
Trait Loci

The purpose of this study was to determine the genetic relationship between salt tolerance during seed germination and vegetative growth in tomato by comparing quantitative trait loci (QTLs) which confer salt tolerance at these two developmental stages. A salt-sensitive Lycopersicon esculentum line (NC84173; maternal and recurrent parent) was hybridized with a salt-tolerant accession (LA722) of Lycopersicon pimpinellifolium, and BC1 and BC1S1 populations were developed. The BC1 population was used for RFLP mapping and the BC1S1 population for evaluation of salt tolerance during germination and vegetative growth. The results indicated the presence of a small but significant correlation (r = -0.22, p < 0.05) between rate of seed germination and the percentage of plant survival under salt stress. Seven and five QTLs were identified for salt tolerance during seed germination and vegetative growth, respectively. While in most cases the location of QTLs for germination was different from that for vegetative growth, there were some coincidences in QTL locations; this was consistent with the small phenotypic correlation observed between the two traits. The overall results indicated that, in these tomato genetic materials, salt tolerance during seed germination was independent of that during vegetative growth. However, simultaneous improvement of tolerance at the two developmental stages should be possible through marker-assisted selection and breeding.Key words: Lycopersicon esculentum, L. pimpinellifolium, salt tolerance, seed germination vegetative growth, restriction fragment length polymorphism (RFLP), quantitative trait loci (QTLs).

scholarly journals Assessment of 16 Peanut (Arachis hypogaea L.) CSSLs Derived from an Interspecific Cross for Yield and Yield Component Traits: QTL Validation

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 583 ◽  
Author(s):  
Hodo-Abalo Tossim ◽  
Joel Romaric Nguepjop ◽  
Cyril Diatta ◽  
Aissatou Sambou ◽  
Maguette Seye ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Seed Size ◽  
Quantitative Trait ◽  
Interspecific Cross ◽  
Yield Component ◽  
Recurrent Parent ◽  
Qtl Validation ◽  
Advanced Backcross ◽  
Lattice Design ◽  
Trait Loci

Cultivated peanut is an allotetraploid (2n = 4× = 40) with narrow genetic diversity. In previous studies, we developed an advanced backcross quantitative trait loci (AB-QTL) population from the cross between the synthetic allotetraploid ((Arachis ipaensis × Arachis duranensis)4×) and the cultivated variety Fleur11, and mapped several quantitative trait loci (QTLs) involved in yield and yield components. We also developed a chromosome segment substitution line (CSSL) population as a way to mendelize the QTLs and analyzing their effects. In this study, 16 CSSLs were used for assessing the contribution of wild alleles in yield performance and stability across environments, as well as validating QTLs for pod and seed size. The CSSLs and the recurrent parent Fleur11, used as a check, were assessed using an alpha lattice design in three locations during two consecutive rainy seasons in Senegal, totaling six environments. Our results showed that the chromosome segments from the wild species, in general, have no yield disadvantage and contributed positive variation to yield-related traits. Most of the QTLs detected for pod and seed size in the AB-QTL on linkage groups A07, A08, A09, and B06 were also found in the CSSLs, showing that the CSSLs used in this study are accurate material for QTL validation. Several new QTLs have also been identified. Two CSSLs (12CS_031 and 12CS_069) showed consistently higher pod and seed size than Fleur11 in all environments, suggesting that the QTLs were consistent and stable. Our study opens the way for pyramiding these QTLs for peanut improvement.

scholarly journals Quantitative Trait Loci (QTL) Analysis of Fruit and Agronomic Traits of Tropical Pumpkin (Cucurbita moschata) in an Organic Production System

Horticulturae ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 14 ◽  
Author(s):  
Angel R. Del Valle Echevarria ◽  
Alexandra Campbell ◽  
Theodore J. K. Radovich ◽  
Tia Silvasy ◽  
Sarah Moore ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Agronomic Traits ◽  
Consumer Preferences ◽  
Organic Production ◽  
Recurrent Parent ◽  
Cucurbita Moschata ◽  
Significant Qtls ◽  
Trait Loci ◽  
Organically Grown

Interest in the development of organically grown vegetable crops has risen over the past decades due to consumer preferences. However, most crops that have desirable consumer traits have been bred in conventional growing conditions, and their transfer to an organic setting is challenging. Here, the organically grown Hawaiian pumpkin (Cucurbita moschata) accession ‘Shima’ was crossed with the conventionally grown Puerto Rican variety ‘Taina Dorada’ to develop a backcross (BC1) population, where ‘Shima’ was the recurrent parent. A total of 202 BC1 (‘Shima’ X F1) progenies were planted in a certified organic field, and twelve traits were evaluated. We used genotype-by-sequencing (GBS) to identify the Quantitative Trait Loci (QTL) associated with insect tolerance along with commercially desirable traits. A total of 1582 single nucleotide polymorphisms (SNPs) were identified, from which 711 SNPs were used to develop a genetic map and perform QTL mapping. Reads associated with significant QTLs were aligned to the publicly available Cucurbita moschata genome and identified several markers linked to genes that have been previously reported to be associated with that trait in other crop systems, such as melon (Cucumis melo L.). This research provides a resource for marker-assisted selection (MAS) efforts in Cucurbita moschata, as well as serving as a model study to improve cultivars that are transitioning from a conventional to an organic setting.

Identification of bioconversion quantitative trait loci in the interspecific cross Sorghum bicolor × Sorghum propinquum

2013 ◽  
Vol 126 (9) ◽  
pp. 2367-2380 ◽  
Author(s):  
Joshua P. Vandenbrink ◽  
Valorie Goff ◽  
Huizhe Jin ◽  
Wenqian Kong ◽  
Andrew H. Paterson ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Sorghum Bicolor ◽  
Quantitative Trait ◽  
Interspecific Cross ◽  
Sorghum Propinquum ◽  
Trait Loci
Sign in / Sign up

Export Citation Format

Share Document