Molecular phylogeny of the genus Hordeum using three chloroplast DNA sequences

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1157-1166 ◽  
Author(s):  
Tomotaro Nishikawa ◽  
Björn Salomon ◽  
Takao Komatsuda ◽  
Roland von Bothmer ◽  
Koh-ichi Kadowaki

The genus Hordeum consists of three cytotypes (2x, 4x, and 6x). Its reproductive isolation has been incomplete between closely related species and hence the genetic relationship is reticulate and complex. We used 32 taxa of Hordeum and three chloroplast DNA sequences, matK, atpB–rbcL, and trnL–trnF, in the current study. Molecular phylogenetic analysis based on sequence data of the three chloroplast DNA regions clearly demonstrated genetic relationships among taxa and origin of polypoids. The formation of H. secalinum likely involved hybridization between Hordeum marinum subsp. marinum and a Eurasian diploid possessing the H genome. The formation of hexaploid Hordeum brachyantherum involved hybridization between tetraploid H. brachyantherum and diploid H. marinum subsp. gussoneanum. The formation of three tetraploids, H. brachyantherum, Hordeum jubatum, and Hordeum guatemalense, probably involved hybridization between H. brachyantherum subsp. californicum and an altered H genome diploid. The formation of Hordeum arizonicum involved the two taxa Hordeum pusillum and H. jubatum.Key words: chloroplast DNA, phylogeny, Hordeum, barley.

2020 ◽  
Author(s):  
Patrick J. Brownsey ◽  
Daniel J. Ohlsen ◽  
Lara D. Shepherd ◽  
Whitney L. M. Bouma ◽  
Erin L. May ◽  
...  

Five indigenous species of Pellaea in Australasia belong to section Platyloma. Their taxonomic history is outlined, morphological, cytological and genetic evidence for their recognition reviewed, and new morphological and chloroplast DNA-sequence data provided. Australian plants of P. falcata (R.Br.) Fée are diploid and have longer, narrower pinnae than do New Zealand plants previously referred to P. falcata, which are tetraploid. Evidence indicates that P. falcata does not occur in New Zealand, and that collections so-named are P. rotundifolia (G.Forst.) Hook. Chloroplast DNA sequences are uninformative in distinguishing Australian P. falcata from New Zealand P. rotundifolia, but show that Australian P. nana is distinct from both. Sequence data also show that Australian and New Zealand populations of P. calidirupium Brownsey & Lovis are closely related, and that Australian P. paradoxa (R.Br.) Hook. is distinct from other Australian species. Although P. falcata is diploid and P. rotundifolia tetraploid, P. calidirupium, P. nana (Hook.) Bostock and P. paradoxa each contain multiple ploidy levels. Diploid populations of Pellaea species are confined to Australia, and only tetraploids are known in New Zealand. Evolution of the group probably involved hybridisation, autoploidy, alloploidy, and possibly apomixis. Further investigation is required to resolve the status of populations from Mount Maroon, Queensland and the Kermadec Islands.


2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Mulatu Geleta ◽  
Tomas Bryngelsson

DNA sequence data from the internal transcribed spacer of nuclear ribosomal DNA and eight chloroplast DNA regions were used to investigate haplotypic variation and population genetic structure of the Afroalpine giant lobelia,Lobelia rhynchopetalum.The study was based on eight populations sampled from two mountain systems in Ethiopia. A total of 20 variable sites were obtained, which resulted in 13 unique haplotypes and an overall nucleotide diversity (ND) of 0.281 ± 0.15 and gene diversity (GD) of 0.85 ± 0.04. Analysis of molecular variance (AMOVA) revealed a highly significant variation (P<0.001) among populations (FST), and phylogenetic analysis revealed that populations from the two mountain systems formed their own distinct clade with >90% bootstrap support. Each population should be regarded as a significant unit for conservation of this species. The primers designed for this study can be applied to anyLobeliaand other closely related species for population genetics and phylogenetic studies.


Phytotaxa ◽  
2016 ◽  
Vol 277 (2) ◽  
pp. 146
Author(s):  
CHEN REN

Indocypraea (Asteraceae-Heliantheae-Ecliptinae) is a newly-erected monotypic genus, containing only I. montana. Historically, this species has been variously included in Verbesina, Wedelia, and Wollastonia, and its position has never been investigated from a molecular phylogenetic perspective. Based on chloroplast DNA sequences, I revealed that I. montana is only remotely related to these genera, and is sister to Synedrella with strong support. This topology is also significantly favored by the statistical topology tests. The generic status of Indocypraea is further supported by the achenes brownish yellow and densely dotted with white or yellowish white mottles, and a base chromosome number of x = 37, both of which are distinct in the subtribe Ecliptinae. Additionally, I clarify the identity of Wedelia wallichii var. megalantha, and synonymize it with I. montana.


2021 ◽  
Vol 44 (1) ◽  
Author(s):  
TAKAYUKI OHGUE ◽  
HIROYUKI AKIYAMA ◽  
HIROSHI SUZUKI-AZUMA ◽  
HIDETOSHI NAGAMASU

The phylogenetic circumscription and taxonomic status of the genus Pohlia in the Mniaceae sensu lato was investigated based on chloroplast DNA sequences (rbcL, rps4, and trnL-F), with a focus on species occurring in Japan. The maximum likelihood and Bayesian inference analyses of sequences obtained from 34 species of Mniaceae s.l., including 13 Pohlia species, suggested that the genus Pohlia and the family Mielichhoferiaceae are not monophyletic in their present circumscription, but confirmed that the family Mniaceae is monophyletic in its traditional sense. These results are congruent with previous molecular phylogenetic studies. Three distinct clades were recognized in the Mielichhoferiaceae, almost corresponding to three sections of Pohlia (Pohlia, Cacodon and Apalodictyon). One of them branched off first within the Mniaceae s.l., and the other two were sister to the remainder of the Mniaceae s.l. The single included Schizymenium formed a monophyletic group with Pohlia sect. Pohlia and Epipterygium with Pohlia sect. Apalodictyon, confirming the results of previous studies. The results indicate that the taxonomic status of the genus Pohlia and family Mielichhoferiaceae are in need of revision. Phylogenetic analyses nested the accessions of P. camptotrachela within P. annotina and P. flexuosa clades, highlighting the need for taxonomic revision of Japanese propaguliferous Pohlia species.


Tropics ◽  
2003 ◽  
Vol 13 (2) ◽  
pp. 79-87 ◽  
Author(s):  
Dayananda Thawalama GAMAGE ◽  
Morley de SILVA ◽  
Akira YOSHIDA ◽  
Alfred E. SZMIDT ◽  
Tsuneyuki YAMAZAKI

2012 ◽  
Vol 298 (6) ◽  
pp. 1151-1165 ◽  
Author(s):  
Marina Grabiele ◽  
Laura Chalup ◽  
Germán Robledo ◽  
Guillermo Seijo

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