Nuclear DNA content and base composition in 28 taxa of Musa

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 622-627 ◽  
Author(s):  
K Kamaté ◽  
S Brown ◽  
P Durand ◽  
J -M Bureau ◽  
D De Nay ◽  
...  
Keyword(s):  
Dna Content ◽  
Petunia Hybrida ◽  
Base Composition ◽  
Nuclear Dna ◽  
Internal Standard ◽  
Nuclear Dna Content ◽  
Leaf Sheath ◽  
Musa Balbisiana ◽  
A Genome ◽  
Aa Genome

The nuclear DNA content of 28 taxa of Musa was assessed by flow cytometry, using line PxPC6 of Petunia hybrida as an internal standard. The 2C DNA value of Musa balbisiana (BB genome) was 1.16 pg, whereas Musa acuminata (AA genome) had an average 2C DNA value of 1.27 pg, with a difference of 11% between its subspecies. The two haploid (1C) genomes, A and B, comprising most of the edible bananas, are therefore of similar size, 0.63 pg (610 million bp) and 0.58 pg (560 million bp), respectively. The genome of diploid Musa is thus threefold that of Arabidopsis thaliana. The genome sizes in a set of triploid Musa cultivars or clones were quite different, with 2C DNA values ranging from 1.61 to 2.23 pg. Likewise, the genome sizes of tetraploid cultivars ranged from 1.94 to 2.37 pg (2C). Apparently, tetraploids (for instance, accession I.C.2) can have a genome size that falls within the range of triploid genome sizes, and vice versa (as in the case of accession Simili Radjah). The 2C values estimated for organs such as leaf, leaf sheath, rhizome, and flower were consistent, whereas root material gave atypical results, owing to browning. The genomic base composition of these Musa taxa had a median value of 40.8% GC (SD = 0.43%).Key words: Musa, cytometry, banana.

scholarly journals NUCLEAR DNA CONTENT OF DENDROBIUM ORCHID SPECIES AS DETERMINED BY LASER FLOW CYTOMETRY

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 322c-322
Author(s):  
W.E. Jones ◽  
A.R. Kuehnle ◽  
K. Arumuganathan
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Evolutionary Relationship ◽  
Internal Standard ◽  
Nuclear Dna Content ◽  
Orchid Species ◽  
Group Assignment ◽  
Taxonomic Groups

Flow cytometry (FC) has proven to be an efficient and reliable method to estimate nuclear DNA content (genome size) in quantifiable units useful for genetic and molecular biology studies. This method also makes possible determination of the variation in nuclear DNA content between related taxa, which gives insights into the process of speciation. In this study, DNA content was determined in nuclei isolated from leaves of 21 Dendrobium species representing each of the major taxonomic groups used in the Univ. of Hawaii breeding program. Nuclei were mechanically isolated, stained with the nucleic acid-specific fluorochrom propidium iodide, and DNA content determined using a Coulter Epics 753 laser flow cytometer. Chicken erythrocyte nuclei (2C = 2.33 pg DNA) were used as an internal standard for direct comparative measurement. The mean diploid genome (2C) values for Dendrobium species ranged from 3.36 to 5.06 pg. Genome sizes were evaluated for possible use as discrete characters for taxonomic group assignment and compared to previous data on breeding compatibility and evolutionary relationship between species.

scholarly journals Nuclear DNA Content, Base Composition, and Cytogenetic Characterization of Christia obcordata

2013 ◽  
Vol 138 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Hamidou F. Sakhanokho ◽  
Nurul Islam-Faridi
Keyword(s):  
Ribosomal Rna ◽  
Dna Content ◽  
Nuclear Dna ◽  
Organizer Region ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Gene Families ◽  
Root Tip ◽  
Ribosomal Rna Gene ◽  
A Genome

Christia obcordata is an intriguing small-sized house plant with unusual and attractive features such as its striped leaves. Because very little is known about the plant, we conducted an investigation of its genome and chromosomes. The number of chromosomes was determined using a protoplast technique to prepare root tip chromosome spread and was found to be 2n = 2x = 20. Flow cytometry was used to determine nuclear DNA content (1C = 0.65 pg = 634.4 Mb) for C. obcordata and AT/GC composition was shown to be AT% = 62.8% ± 0.0% and GC% = 37.2% ± 0.0%. Finally, fluorescent in situ hybridization was used to locate ribosomal RNA gene families in C. obcordata. Ribosomal RNA gene families, viz. 18S-28S and 5S rDNA, are unique cytomolecular landmarks that provide valuable information about the evolutionary organization of a genome. We have identified one locus each of 18S-28S and 5S rDNA. The 18S-28S rDNA is located in the subterminal position on the secondary constriction region [also known as the nucleolus organizer region (NOR)] and the 5S rDNA is located interstitially close to a centromeric position. The basic information gathered in this study on C. obcordata will be helpful in understanding the genetics of this species.

scholarly journals Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

2017 ◽  
Vol 8 ◽  
Author(s):  
A. Mondal S.K. Ghosal ◽  
T. Pal Kalyan Kumar De
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Cane Yield ◽  
Sugarcane Varieties ◽  
Yield Information ◽  
A Genome ◽  
2C Dna Content

<p>In the present study, 2C DNA content and the genome sizes (in picograms-pg and megabase pairs-Mbp respectively) of 19 promising commercial varieties of sugarcane, the derivatives of man-made interspecific hybrids between cultivated and wild species were analyzed using flow cytometry. In this work, 2C nuclear DNA content was determined. Knowing the 2C nuclear DNA content, the unknown chromosome numbers of the varieties could be predicted. Large differences (65 % variation) in DNA content (2C) of 19 varieties were detected, ranging, from 3.80 pg to 10.96 pg, which corresponds to a genome size ranging from 3724.00 Mbp to 10740.80 Mbp due to the variation of ploidy level and are considered the most complex genomes among crop plants. However, the relationship between chromosome number and genome size was highly significant (P &lt; 0.001). In the present study, internode diameter, Sugar juice content and cane yield/ha are also positively correlated with DNA content. The estimated genome sizes would also yield information critical for sugarcane breeding and genome sequencing programs.                                </p><p><strong>Keywords</strong><strong>: </strong>Genome size, Sugarcane varieties, Flow cytometry, DNA content.</p>

scholarly journals Nuclear DNA Content and Ploidy Levels in the Genus Ipomoea

1994 ◽  
Vol 119 (1) ◽  
pp. 110-115 ◽  
Author(s):  
Peggy Ozias-Akins ◽  
Robert L. Jarret
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Large Scale ◽  
Nuclear Dna ◽  
Internal Standard ◽  
Nuclear Dna Content ◽  
Ploidy Levels ◽  
Genetic Structure Of Populations ◽  
Chicken Erythrocytes ◽  
Nuclei Ploidy Level

The nuclear DNA content of 53 accessions from 24 Ipomoea (Convolvulaceae) species, including four sweetpotato cultivars, was determined by flow cytometry of DAPI-stained nuclei. Ploidy level and DNA content were significantly correlated within the genus, but more highly so within species that contained multiple cytotypes. DNA content of cultivated Z. batatas (L.) Lam. (4.8 to 5.3 pg/2C nucleus) and feral tetraploid I. batatas (3.0 to 3.5 pg/2C nucleus) was estimated from the known DNA content of chicken erythrocytes (2.33 pg), which were used as an internal standard. Tetraploid forms of Z. cordato-triloba Dennstedt also were identified. Ploidy analysis using flow cytometry is rapid and suitable for large-scale experiments such as studying the genetic structure of populations of Z. batatas and related species. Chemical name used: 4′,6-diamidino-2-phenylindole (DAPI).

Genome size and karyotype length in some interstitial polychaete species of the genus Ophryotrocha (Dorvilleidae)

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 652-657 ◽  
Author(s):  
G. Sella ◽  
C. A. Redi ◽  
L. Ramella ◽  
R. Soldi ◽  
M. C. Premoli
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
Evolutionary Biology ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Egg Cell ◽  
Cell Diameter ◽  
Time Interval ◽  
Interspecific Differences ◽  
A Genome

Interstitial polychaetes of the genus Ophryotrocha are very small, progenetic, and morphologically very similar. These worms have been widely used in evolutionary biology and sexuality studies. To have a better insight into the karyological evolution of this genus, we measured the total karyotypic length and the 2C nuclear DNA content of the nine best-known species of this genus. No interspecific differences were observed in karyotypic lengths, apart from that of O. gracilis, which was significantly greater than the karyotypic length of five of the nine species. The genome size (i.e., 1C DNA content calculated from 2C DNA content) in eight of the nine species is about 0.4 pg, irrespective of the chromosome number. A group of four gonochoric and morphologically indistinguishable species, with 2n = 6 metacentric chromosomes, appears to be heterogeneous with regard to its DNA content, because one of the species, O. macrovifera, has a genome twice the size of that of the other three species. A hermaphroditic species, O. hartmanni, has a genome three times that size. No correlation has been observed between genome size and body size, egg cell diameter, or time interval from egg fertilization to sexual maturity. The basic genome size of 0.4 pg is among the lowest recorded in invertebrates. Hypotheses about selective pressures that maintain such a low amount of nuclear DNA in this genus are discussed.Key words: genome size, meiofauna, Ophryotrocha, Polychaeta.

Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies

2002 ◽  
Vol 104 (2) ◽  
pp. 505-512 ◽  
Author(s):  
S. Siljak-Yakovlev ◽  
M. Cerbah ◽  
J. Coulaud ◽  
V. Stoian ◽  
S. C. Brown ◽  
...  
Keyword(s):  
Picea Abies ◽  
Dna Content ◽  
Base Composition ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Picea Omorika

Nuclear DNA content and base composition in 28 taxa of Musa

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 622-627 ◽  
Author(s):  
K. Kamaté ◽  
S. Brown ◽  
P. Durand ◽  
J.-M. Bureau ◽  
D. De Nay ◽  
...  
Keyword(s):  
Dna Content ◽  
Base Composition ◽  
Nuclear Dna ◽  
Nuclear Dna Content

Nuclear DNA content of 11 fungal species in Glomales

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 422-428 ◽  
Author(s):  
Michel Hosny ◽  
Vivienne Gianinazzi-Pearson ◽  
Hubert Dulieu
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Internal Standard ◽  
Nuclear Dna Content ◽  
Fungal Species ◽  
Dapi Staining ◽  
Interspecific Variations ◽  
Propidium Iodide Staining ◽  
The Difference

The nuclear DNA content of 11 species of Glomales was evaluated by flow cytometry after DAPI staining relative to Gigaspora margarita, which was used as internal standard. The nuclear DNA content of this species was calibrated by propidium iodide staining relative to chicken red blood cells. A correction was applied when the difference in AT content of the DNA was significant between a sample and the standard. A single unimodal peak of fluorescence was observed for nuclei from the quiescent spores of the 11 fungal species studied. It was considered that this peak corresponded to the amount of DNA in the genome of each species. Important interspecific variations in DNA content per nucleus (1- to 8-fold) were observed among four species of the genus Scutellospora.Key words: nucleus, DNA content, flow cytometry, spore, Glomales.

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer
Sign in / Sign up

Export Citation Format

Share Document