DNA content, rDNA loci, and DAPI bands reflect the phylogenetic distance between Lathyrus species

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1027-1032 ◽  
Author(s):  
Hoda Badry Mohammed Ali ◽  
Armin Meister ◽  
Ingo Schubert
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Rrna Genes ◽  
Phylogenetic Distance ◽  
Base Pairs ◽  
Rdna Loci ◽  
Dapi Banding ◽  
25S Rdna ◽  
Dapi Banding Pattern

The nuclear DNA content, the proportion of A+T base pairs, the chromosomal positions of 5S and 25S rRNA genes, as well as of DAPI (4',6-diamidino-2-phenylindole) bands are described for seven species belonging to three different sect ions of the genus Lathyrus. These data and chromosome measurements allowed to establish precise idiograms and to discriminate most of the chromosome pairs of the seven species. The karyotypic features correlate well with the phylogenetic distances between these species.Key words: Lathyrus karyotypes, 5S/25S rDNA loci, FISH, DAPI banding pattern, nuclear DNA content.

Chromatin characterization by banding techniques, in situ hybridization, and nuclear DNA content in Cicer L. (Leguminosae)

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 258-265 ◽  
Author(s):  
I. Galasso ◽  
D. Pignone ◽  
M. Frediani ◽  
M. Maggiani ◽  
R. Cremonini
Keyword(s):  
In Situ Hybridization ◽  
Dna Content ◽  
Nuclear Dna ◽  
Hybrid Sterility ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Rrna Genes ◽  
Evolutionary Pathway ◽  
C Banding

The karyotypes of three accessions, one each from three annual species of the genus Cicer, namely Cicer arietinum, Cicer reticulation, and Cicer echinospermum, were examined and compared using C-banding, the fluorochromes chromomycin A3, DAPI, and Hoechst 33258, in situ hybridization of the 18S–5.8S–25S and 5S rDNA sequences, and silver staining. The nuclear DNA content of the three species and the amount of heterochromatin were also determined. The results suggest an evolutionary pathway in which C. reticulatum is the ancestral species from which both C. arietinum and C. echinospermum are derived with the loss of one pair of satellites; subsequently, C. echinospermum further differentiated by the accumulation of chromosomal rearrangement(s) that gave rise to a hybrid sterility barrier. Key words : Cicer, C-banding, fluorochromes, Ag staining, rRNA genes.

scholarly journals Chromosome and Ploidy Analysis of Winter Hardy Hibiscus Species by FISH and Flow Cytometry

2021 ◽  
Author(s):  
Deen Mohammad Deepo ◽  
Islam MD Mazha ◽  
Yoon-Jung Hwang ◽  
Hong-Yul Kim ◽  
Chang Kil Kim ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Ploidy Level ◽  
Dna Content ◽  
18S Rdna ◽  
Nuclear Dna ◽  
Cytogenetic Study ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Rdna Loci ◽  
2C Dna Content

Abstract Determination of nuclear DNA content, genome sizes, and ploidy level and, information on cytogenetic characteristics are all prerequisite of modern plant breeding. However, cytogenetic study of Hibiscus species is often hampered by the similarity in morphology and by the small size of mitotic chromosomes. The goal of the study was to ascertain the chromosome number, karyomorphology, distribution of 5S and 18S rDNA loci, chromosome length, and centromere positions as well as the ploidy level, genome sizes, 2C-DNA content of winter-hardy Hibiscus: H. syriacus ‘Saejamyung’, H. sinosyriacus ‘Seobong’, H. moscheutos ‘Luna Red’ and, H. paramutabilis. 5S rDNA and 18S rDNA loci were detected by fluorescence in situ hybridization (FISH). According to the FISH results, there are two 5S rDNA loci in H. syriacus, H. sinosyriacus, and H. moscheutos, and four 5S rDNA loci in H. paramutabilis. The range in length of somatic chromosomes in H. syriacus, H. sinosyriacus, H. moscheutos, and H. paramutabilis is 2.66- 7.06, 3.18-7.31, 2.91-5.23, and 4.75-7.60 µm, respectively. The 2C-DNA content of H. syriacus, H. sinosyriacus, and H. moscheutos are very similar, the amount was 4.06, 4.11, and, 2.06 pg, respectively whereas, H. paramutabilis has nearly double and the amount was 4.18 pg. These findings will contribute to the detailed cytogenetic assessment of Hibiscus and thus benefit plant breeding in this genus.

scholarly journals Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg

2011 ◽  
Vol 77 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Bożena Kolano ◽  
Dorota Siwińska ◽  
Jolanta Małuszyńska
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chenopodium Album ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Somatic Tissue ◽  
Rdna Sites ◽  
25S Rdna ◽  
The Individual

Two cytotypes of <em>Chenopodium album</em>, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid <em>C. album</em> and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of <em>C. album</em> revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid <em>C. album</em> was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

Changes in chromosome structure, mitotic activity and nuclear DNA content from cells of Allium Test induced by bark water extract of Uncaria tomentosa (Willd.) DC

2006 ◽  
Vol 107 (2) ◽  
pp. 211-221 ◽  
Author(s):  
Mieczysław Kuraś ◽  
Julita Nowakowska ◽  
Elwira Śliwińska ◽  
Radosław Pilarski ◽  
Renata Ilasz ◽  
...  
Keyword(s):  
Mitotic Activity ◽  
Dna Content ◽  
Nuclear Dna ◽  
Chromosome Structure ◽  
Water Extract ◽  
Nuclear Dna Content ◽  
Allium Test ◽  
Uncaria Tomentosa
Sign in / Sign up

Export Citation Format

Share Document