Molecular contribution to selection of intergeneric hybrids between sugarcane and the wild species Erianthus arundinaceus

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1033-1037 ◽  
Author(s):  
George Piperidis ◽  
Mandy J Christopher ◽  
Bernie J Carroll ◽  
Nils Berding ◽  
Angélique D'Hont
Keyword(s):  
In Situ Hybridization ◽  
5S Rdna ◽  
Saccharum Officinarum ◽  
Genomic In Situ Hybridization ◽  
Early Screening ◽  
Selfed Progeny ◽  
Cross Hybridization ◽  
Rdna Cluster ◽  
Erianthus Arundinaceus

Erianthus arundinaceus has great potential as a germplasm source for better ratoonability, vigour, tolerance to environmental stresses, and disease resistance in sugarcane. Many unsuccessful attempts have been made to introduce these characters into modern sugarcane cultivars. We report on significant progress made since molecular tools were implemented. Sequence-tagged PCR, revealing size variation in the 5S rDNA cluster, was performed on intact leaf tissue to identify genuine hybrids six weeks after germination. This early screening of seedlings avoids the loss of genuine hybrids due to competition with selfed progeny. Of 96 crosses made involving female Saccharum officinarum or sugarcane cultivars (Saccharum spp.) and male E. arundinaceus, 26 were fertile producing 1328 seedlings. Thirty-seven genuine hybrids were unequivocally identified but only 19 have survived. Genuine hybrids were produced from only three crosses, all involving S. officinarum as the female parent. Chromosome elimination was observed in all seven hybrids analyzed using genomic in situ hybridization (GISH). Very little cross-hybridization was observed between the genomes of the two species after GISH, confirming recent molecular studies which showed that E. arundinaceus is quite distant from the genus Saccharum. The major limitation in the introgression of E. arundinaceus resides now in the apparent sterility of the hybrids.Key words: sugarcane, Erianthus, intergeneric hybrid, genomic in situ hybridization, 5S rDNA, sequence-tagged PCR.

scholarly journals Chromosome behavior during meiosis in pollen mother cells from Saccharum officinarum × Erianthus arundinaceus F1 hybrids

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xueting Li ◽  
Fei Huang ◽  
Jin Chai ◽  
Qiusong Wang ◽  
Fan Yu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
5S Rdna ◽  
Saccharum Officinarum ◽  
F1 Hybrids ◽  
45S Rdna ◽  
Pollen Mother Cells ◽  
Erianthus Arundinaceus ◽  
Rdna Sites ◽  
Mother Cells

Abstract Background In recent years, sugarcane has attracted increasing attention as an energy crop. Wild resources are widely used to improve the narrow genetic base of sugarcane. However, the infertility of F1 hybrids between Saccharum officinarum (S. officinarum) and Erianthus arundinaceus (E. arundinaceus) has hindered sugarcane breeding efforts. To discover the cause of this infertility, we studied the hybridization process from a cytological perspective. Results We examined the meiotic process of pollen mother cells (PMCs) in three F1 hybrids between S. officinarum and E. arundinaceus. Cytological analysis showed that the male parents, Hainan 92–77 and Hainan 92–105, had normal meiosis. However, the meiosis process in F1 hybrids showed various abnormal phenomena, including lagging chromosomes, micronuclei, uneven segregation, chromosome bridges, and inability to form cell plates. Genomic in situ hybridization (GISH) showed unequal chromatin distribution during cell division. Interestingly, 96.70% of lagging chromosomes were from E. arundinaceus. Furthermore, fluorescence in situ hybridization (FISH) was performed using 45S rDNA and 5S rDNA as probes. Either 45S rDNA or 5S rDNA sites were lost during abnormal meiosis, and results of unequal chromosomal separation were also clearly observed in tetrads. Conclusions Using cytogenetic analysis, a large number of meiotic abnormalities were observed in F1. GISH further confirmed that 96.70% of the lagging chromosomes were from E. arundinaceus. Chromosome loss was found by further investigation of repeat sequences. Our findings provide insight into sugarcane chromosome inheritance to aid innovation and utilization in sugarcane germplasm resources.

Relationships among 3 Kochia species based on PCR-generated molecular sequences and molecular cytogenetics

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1104-1115 ◽  
Author(s):  
B S Lee ◽  
M Y Kim ◽  
R R.-C Wang ◽  
B L Waldron
Keyword(s):  
United States ◽  
In Situ Hybridization ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
5S Rdna ◽  
The United States ◽  
Cross Hybridization ◽  
Forage Kochia

Forage kochia (Kochia prostrata ssp. virescens 'Immigrant' is native to the arid and semiarid regions of central Eurasia. It was introduced into the United States in 1966 as PI 314929 and released as a perennial forage shrub in 1984. Kochia americana is a perennial native to the United States, whereas Kochia scorparia is an introduced annual species that became a weed. To assess both the breeding potential and the possibility of genetic contamination, relationships among the 3 Kochia species were analyzed using random amplified polymorphic DNA (RAPD) markers, sequence tagged site (STS) marker sequences of the chloroplast NADH dehydrogenase gene (ndhF), genomic in situ hybridization (GISH), and multicolor fluorescence in situ hybridization (MC-FISH). Seventy decamer random primers yielded 458 polymorphic bands from 9 plants of K. americana, 20 plants of K. prostrata, and 7 plants of K. scoparia. Fifty-four and 55 species-specific RAPD markers were identified for K. americana and K. prostrata, whereas 80 RAPD markers were specific to K. scoparia. Based on the presence or absence of informative RAPD markers, the 3 species always grouped into 3 distinct clusters in a NTSYSpc2.01b-generated dendrogram. The same relationships were found among the 3 Kochia species based on ndhF DNA sequence divergence. Using a set of 7 STS markers that can identify each Kochia species, we did not find a single interspecific hybrid from artificial hybridizations among the 3 Kochia species. In GISH studies, chromosomes of 1 species fluoresced in green only when they were probed by genomic DNA of the same species. Cross-hybridization by genomic DNA of another species was not observed. In FISH studies using pTa71 (for 18S–5.8S–26S rDNAs) and pScT7 (for 5S rDNA) as probes, there were 1, 1 and 3 pTa71 sites and 2, 1, and 1 pScT7 sites in each haplome of K. prostrata, K. americana, and K. scoparia, respectively. It is concluded that these 3 Kochia species are so genomically distinct that gene introgression among them would be extremely rare.Key words: RAPD, STS, ndhF, GISH, FISH, mixoploidy, forage kochia.

scholarly journals Multi-color Genomic In Situ Hybridization Identifies Parental Chromosomes in Somatic Hybrids of Diospyros kaki and D. glandulosa

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 184-186 ◽  
Author(s):  
Young-A Choi ◽  
R. Tao ◽  
K. Yonemori ◽  
A. Sugiura
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Fluorescein Isothiocyanate ◽  
Genomic In Situ Hybridization ◽  
Diospyros Kaki ◽  
Cross Hybridization ◽  
Total Dna ◽  
Labeled Probe ◽  
Chromosome Regions

Multi-color genomic in situ hybridization (MCGISH) was performed for mitotic cells of the somatic hybrids of Diospyros kaki (2n = 6x = 90) and D. glandulosa (2n = 2x = 30). Total DNA of D. kaki and D. glandulosa were isolated and labeled with biotin-16-UTP and digoxigenin (DIG)-11-UTP, respectively. The labeled DNAs were used as probes to differentiate parental chromosomes. The biotin-labeled probe was detected with avidin-rhodamine, and the DIG-labeled probe was detected with anti-DIG-FITC (fluorescein isothiocyanate). Ninety chromosomes from D. kaki that showed reddish-orange and 30 chromosomes from D. glandulosa that showed greenish-yellow were observed under a fluorescence microscope. Some chromosomes showed cross-hybridization with both probes at their terminal or other chromosome regions. These results indicated that MCGISH could be used to analyze genomes of Diospyros species whose chromosomes are small and numerous.

Genome analysis of seven species of Kengyilia (Triticeae: Poaceae) with FISH and GISH

Genome ◽  
2013 ◽  
Vol 56 (11) ◽  
pp. 641-649 ◽  
Author(s):  
Quanwen Dou ◽  
Richard R.-C. Wang ◽  
Yuting Lei ◽  
Feng Yu ◽  
Yuan Li ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genetic Relationships ◽  
5S Rdna ◽  
Common Species ◽  
Genomic In Situ Hybridization ◽  
Genetic Groups ◽  
Molecular Karyotype ◽  
Species Specific

The genome compositions and genetic relationships of seven species of Kengyilia were assessed using a sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) technique. Five species, K. kokonorica, K. rigidula, K. hirsuta, K. grandiglumis, and K. thoroldiana, are native to Qinghai (China). The other two, K. alatavica and K. batalinii, are distributed in Xinjiang (China) and Kyrgyzstan, respectively. Each chromosome could be easily identified using chromosome markers (45S rDNA, 5S rDNA, pAs1, and AAG repeats) by FISH and allocated to the St, P, or Y genome by GISH. Molecular karyotype comparison indicated that K. alatavica and K. batalinii were distinct from the Qinghai species in all three genomes. These results support that the species of Kengyilia from Central Asia and the Qinghai–Tibetan plateau have independent origins. Genomic differentiation was still detected among the species of Kengyilia from Qinghai. Specifically, a common species-specific pericentric inversion was identified in both K. grandiglumis and K. thoroldiana, and an identical St-P non-Robertsonian translocation was frequently detected in K. hirsuta. The Qinghai species formed three genetic groups, K. kokonorica–K. rigidula, K. hirsuta, and K. grandiglumis–K. thoroldiana. The possible role of species-specific inversions and translocations in the evolution of StPY species is discussed.

Synthesis and cytological characterization of trigeneric hybrids of durum wheat with and without Ph1

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Prem P Jauhar ◽  
M Doğramaci ◽  
T S Peterson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Genetic Recombination ◽  
Genomic In Situ Hybridization ◽  
Homoeologous Pairing ◽  
Alien Gene Transfer ◽  
Chromosome 5B ◽  
Alien Gene ◽  
Trigeneric Hybrids

Wild grasses in the tribe Triticeae, some in the primary or secondary gene pool of wheat, are excellent reservoirs of genes for superior agronomic traits, including resistance to various diseases. Thus, the diploid wheatgrasses Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve (2n = 2x = 14; JJ genome) and Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE genome) are important sources of genes for disease resistance, e.g., Fusarium head blight resistance that may be transferred to wheat. By crossing fertile amphidiploids (2n = 4x = 28; JJEE) developed from F1 hybrids of the 2 diploid species with appropriate genetic stocks of durum wheat, we synthesized trigeneric hybrids (2n = 4x = 28; ABJE) incorporating both the J and E genomes of the grass species with the durum genomes A and B. Trigeneric hybrids with and without the homoeologous-pairing suppressor gene, Ph1, were produced. In the absence of Ph1, the chances of genetic recombination between chromosomes of the 2 useful grass genomes (JE) and those of the durum genomes (AB) would be enhanced. Meiotic chromosome pairing was studied using both conventional staining and fluorescent genomic in situ hybridization (fl-GISH). As expected, the Ph1-intergeneric hybrids showed low chromosome pairing (23.86% of the complement), whereas the trigenerics with ph1b (49.49%) and those with their chromosome 5B replaced by 5D (49.09%) showed much higher pairing. The absence of Ph1 allowed pairing and, hence, genetic recombination between homoeologous chromosomes. Fl-GISH analysis afforded an excellent tool for studying the specificity of chromosome pairing: wheat with grass, wheat with wheat, or grass with grass. In the trigeneric hybrids that lacked chromosome 5B, and hence lacked the Ph1 gene, the wheat–grass pairing was elevated, i.e., 2.6 chiasmata per cell, a welcome feature from the breeding standpoint. Using Langdon 5D(5B) disomic substitution for making trigeneric hybrids should promote homoeologous pairing between durum and grass chromosomes and hence accelerate alien gene transfer into the durum genomes.Key words: alien gene transfer, chiasma (xma) frequency, chromosome pairing, fluorescent genomic in situ hybridization (fl-GISH), homoeologous-pairing regulator, specificity of chromosome pairing, wheatgrass.

Genome constitutions ofRoegneriaalashanica,R.elytrigioides,R.magnicaespesandR.grandis(Poaceae: Triticeae) via genomic in-situ hybridization

2010 ◽  
Vol 28 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Hai-Qing Yu ◽  
Chun Zhang ◽  
Chun-Bang Ding ◽  
Hai-Qin Zhang ◽  
Yong-Hong Zhou
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization

Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids

1997 ◽  
Vol 95 (8) ◽  
pp. 1320-1324 ◽  
Author(s):  
R. J. Snowdon ◽  
W. Köhler ◽  
W. Friedt ◽  
A. Köhler
Keyword(s):  
In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization

Analysis of Oriental × Trumpet (OT) Lilium hybrids by genomic in situ hybridization based on ploidy level

2017 ◽  
pp. 253-258
Author(s):  
F. Ramzan ◽  
A. Younis ◽  
K.B. Lim ◽  
S.H. Bae ◽  
M.J. Kwon ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Ploidy Level ◽  
Genomic In Situ Hybridization
Sign in / Sign up

Export Citation Format

Share Document