Ceratotoxins: Female-specific X-linked genes from the medfly, Ceratitis capitata

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 707-711 ◽  
Author(s):  
M Rosetto ◽  
T de Filippis ◽  
M Mandrioli ◽  
A Zacharopoulou ◽  
P Gourzi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gene Family ◽  
Ceratitis Capitata ◽  
Chromosomal Localization ◽  
Fruit Fly ◽  
Mitotic Chromosomes ◽  
Accessory Glands ◽  
First Case ◽  
Female Specific

In this paper, we report the chromosomal localization of ceratotoxins, a gene family encoding antibacterial female-specific peptides from the mediterranean fruit fly Ceratitis capitata. The analysis of both polytene and mitotic chromosomes by in situ hybridization shows that ceratotoxins are the first case of female-specific X-linked genes from the medfly C. capitata. Southern blot analysis reveals that the ceratotoxin gene family is not specifically amplified in the female reproductive accessory glands of C. capitata.Key words: ceratotoxins, female-specific genes, Ceratitis capitata, X chromosome, in situ hybridization.

Molecular characterization and chromosomal localization of female-specific genes from the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae)

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Silvia Ciolfi ◽  
Tiziana de Filippis ◽  
Cristina Torti ◽  
Anna R Malacrida ◽  
Romano Dallai
Keyword(s):  
In Situ Hybridization ◽  
Molecular Characterization ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Accessory Gland ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean ◽  
Female Specific

We report here the molecular characterization of the female-specific FST (female-specific transcript) genes from the Mediterranean fruit fly (medfly) Ceratitis capitata. A genomic clone was isolated, containing a sequence coding for FST. Nucleotide analysis of the clone showed that the gene contains a putative unique intron located in the region encoding the signal peptide. Southern blotting and in situ hybridization analysis on polytene chromosomes suggested the presence of additional genes similar to FST in the genome of the medfly. A novel cDNA clone was isolated from an accessory gland cDNA library, encoding a product that shares 98% identity with the hypothetical translational product of the previously isolated FST cDNA. The novel cDNA was therefore named FST2. The analysis of mitotic and polytene chromosomes by in situ hybridization showed that FST genes map on the left arm of the 4th chromosome of C. capitata.Key words: FST, female-specific genes, C. capitata, medfly, FISH.

scholarly journals Identification and in situ hybridization to mitotic chromosomes of a molecular marker linked to maleness in Anastrepha fraterculus (Wied.)

2020 ◽  
Vol 7 (6) ◽  
pp. 237-240
Author(s):  
Alicia Basso ◽  
Ariane Sonvico
Keyword(s):  
In Situ Hybridization ◽  
Sex Determination ◽  
Present Report ◽  
Fruit Fly ◽  
Mitotic Chromosomes ◽  
Anastrepha Fraterculus ◽  
Determination System ◽  
Sex Determination System ◽  
South American Fruit Fly

The present report shows the molecular identification, isolation and citologically localization of a DNA-sequence from the South American fruit fly Anastrepha fraterculus (DIPTERA: Tephritidae) involved in sex- determination. It belongs to the Tephritidae family, the true fruit flies which are consider a pest of fruit crops. The sex determination system is of vital importance in the genetic control of the fruit fly pest: Sterile Insect Technique which unlike chemical control tactics, is environmentally friendly and does not pose any health concerns. We used in situ hybridization on mitotic chromosomes for localizing the primary sex determination factor in this fruit fly pest. Our results show that in Anastrepha fraterculus the Y chromosome is responsible for sex determination

Fluorescent in situ hybridization (FISH): A useful diagnostic tool for childhood conjunctival melanoma

2021 ◽  
pp. 112067212110307
Author(s):  
Raquel María Moral ◽  
Carlos Monteagudo ◽  
Javier Muriel ◽  
Lucía Moreno ◽  
Ana María Peiró
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Pediatric Population ◽  
Diagnostic Technique ◽  
Histopathological Diagnosis ◽  
Fish Analysis ◽  
Conjunctival Melanoma ◽  
Adequate Treatment ◽  
First Case

Introduction: Conjunctival melanoma is extremely rare in children and has low rates of resolution. Definitive histopathological diagnosis based exclusively on microscopic findings is sometimes difficult. Thus, early diagnosis and adequate treatment are essential to improve clinical outcomes. Clinical case: We present the first case in which the fluorescent in situ hybridization (FISH) diagnostic technique was applied to a 10-year-old boy initially suspected of having amelanotic nevi in his right eye. Based on the 65% of tumor cells with 11q13 (CCND1) copy number gain and 33% with 6p25 (RREB1) gain as measured by the FISH analysis, and on supporting histopathological findings, the diagnosis of conjunctival melanoma could be made. Following a larger re-excision, adjuvant therapy with Mitomycin C (MMC), cryotherapy and an amniotic membrane graft, the patient has remained disease-free during 9 years of long-term follow-up. Case discussion: Every ophthalmologist should remember to consider and not forget the possibility of using FISH analyses during the differential diagnosis of any suspicious conjunctival lesions. Genetic techniques, such as FISH, have led to great advances in the classification of ambiguous lesions. Evidence-based guidelines for diagnosing conjunctival melanoma in the pediatric population are needed to determine the most appropriate strategy for this age group.

scholarly journals Chromosomal localization of human genes for arylamine N-acetyltransferase

1994 ◽  
Vol 297 (3) ◽  
pp. 441-445 ◽  
Author(s):  
D Hickman ◽  
A Risch ◽  
V Buckle ◽  
N K Spurr ◽  
S J Jeremiah ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Chromosomal Localization ◽  
Artificial Chromosome ◽  
Acetylator Phenotype ◽  
Chromosome 8 ◽  
Slow Acetylator ◽  
Human Genes

Arylamine N-acetyltransferase is encoded at two loci, AAC-1 and AAC-2, on human chromosome 8. The products of the two loci are able to catalyse N-acetylation of arylamine carcinogens, such as benzidine and other xenobiotics. AAC-2 is polymorphic and individuals carrying the slow-acetylator phenotype are more susceptible to benzidine-induced bladder cancer. We have identified yeast artificial chromosome clones encoding AAC-1 and AAC-2 and have used the cloned DNAs as fluorescent probes for in situ hybridization. The hybridization patterns allow assignment of AAC-1 and AAC-2 to chromosome 8p21.3-23.1, a region in which deletions have been associated with bladder cancer [Knowles, Shaw and Proctor (1993) Oncogene 8, 1357-1364].

Chromosomal localization of leukocyte interferon gene in the pig (Sus scrofa domestica L.) by in situ hybridization

1986 ◽  
Vol 42 (3) ◽  
pp. 129-132 ◽  
Author(s):  
M. Yerle ◽  
J. Gellin ◽  
G. Echard ◽  
F. Lefevre ◽  
M. Gillois
Keyword(s):  
In Situ Hybridization ◽  
Sus Scrofa ◽  
Chromosomal Localization ◽  
Interferon Gene
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