Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis

Genome ◽  
2000 ◽  
Vol 43 (3) ◽  
pp. 538-549 ◽  
Author(s):  
K R Lindner ◽  
J E Seeb ◽  
C Habicht ◽  
K L Knudsen ◽  
E Kretschmer ◽  
...  
Keyword(s):  
Pink Salmon ◽  
Chromosomal Evolution ◽  
Oncorhynchus Gorbuscha ◽  
Recombination Rates ◽  
Key Words Meiosis ◽  
Length Polymorphisms ◽  
Gynogenetic Diploid ◽  
Amplified Fragment Length Polymorphisms ◽  
Allozyme Loci ◽  
Shared Alleles

We estimated recombination rates between 312 loci and their centromeres in gynogenetic diploid pink salmon (Oncorhynchus gorbuscha) that we produced by initiating development with irradiated sperm and blocking the maternal second meiotic division. Amplified fragment length polymorphisms (AFLPs) were significantly more centromeric than loci identified by three other techniques (allozymes, microsatellites, and PCR using primer sequences from interspersed nuclear elements). The near absence of AFLPs in distal regions could limit their utility in constructing linkage maps. A large proportion of loci had frequency of second division segregation (y) values approaching 1.0, indicating near complete crossover interference on many chromosome arms. As predicted from models of chromosomal evolution in salmonids based upon results with allozyme loci, all duplicated microsatellite loci that shared alleles (isoloci) had y values of nearly 1.0.Key words: meiosis, AFLP, microsatellites, isoloci, Oncorhynchus gorbuscha.

Gene–Centromere Distances of Allozyme loci in Even- and Odd-year Pink Salmon, (Oncorhynchus gorbuscha)

Genetica ◽  
2004 ◽  
Vol 121 (1) ◽  
pp. 1-11 ◽  
Author(s):  
M.P. Matsuoka ◽  
A.J. Gharrett ◽  
R.L. Wilmot ◽  
W.W. Smoker
Keyword(s):  
Pink Salmon ◽  
Oncorhynchus Gorbuscha ◽  
Pink Salmon Oncorhynchus Gorbuscha ◽  
Allozyme Loci

A Demonstration of a 1:1 Correspondence Between Chiasma Frequency and Recombination Using a Lolium perenne/Festuca pratensis Substitution

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 307-314 ◽  
Author(s):  
J King ◽  
L A Roberts ◽  
M J Kearsey ◽  
H M Thomas ◽  
R N Jones ◽  
...  
Keyword(s):  
Lolium Perenne ◽  
Chiasma Frequency ◽  
Chiasma Formation ◽  
Grass Species ◽  
Festuca Pratensis ◽  
Single Chromosome ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Frequency Counts

Abstract A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n = 2x = 14). The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

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