Cloning and characterization of an extremely conserved satellite DNA family from the root-knot nematode Meloidogyne arenaria

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 346-353 ◽  
Author(s):  
Philippe Castagnone-Sereno ◽  
Frédéric Leroy ◽  
Pierre Abad
Keyword(s):  
Satellite Dna ◽  
Potential Role ◽  
Genomic Organization ◽  
Consensus Sequence ◽  
Meloidogyne Arenaria ◽  
Root Knot Nematode ◽  
Phytoparasitic Nematode ◽  
Nematode Genome

A new satellite DNA family, named pMaE, has been cloned from the genome of the phytoparasitic nematode, Meloidogyne arenaria (Nematoda: Tylenchida). It is represented as tandemly repeated sequences with a monomeric unit of 172 bp. The monomers are present at approximately 15 700 copies per haploid genome, and represent about 5.3% of the total genomic DNA. Twenty-seven independent monomers have been cloned and sequenced. The deduced consensus sequence is 70.9% A + T rich, with frequent stretches of A and (or) T. Several direct or inverted sub-repeats are present in the sequence, which may allow the formation of a dyad structure, suggesting some potential role of this repetitive sequence in heterochromatin condensation. The monomers are very homogeneous in sequence, showing on average 1.8% divergence from their consensus sequence. Moreover, Southern blot experiments and sequence analysis of homologous monomers from the genome of geographically distinct M. arenaria populations have shown that this satellite DNA is uniformly distributed and highly conserved within the species. Therefore, it is hypothesized that this unusually low level of variability, either within the genome of a given population or between populations, could be achieved as the result of some highly effective homogenization mechanism acting upon the nematode genome. Key words: genomic organization, Meloidogyne arenaria, satellite DNA.

Cloning and characterization of an extremely conserved satellite DNA family from the root-knot nematode Meloidogyne arenaria

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 346-353 ◽  
Author(s):  
Philippe Castagnone-Sereno ◽  
Frédéric Leroy ◽  
Pierre Abad
Keyword(s):  
Satellite Dna ◽  
Meloidogyne Arenaria ◽  
Root Knot Nematode

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Characterization of indeterminate spleen lesions in primary CT after blunt abdominal trauma: potential role of MR imaging

2014 ◽  
Vol 21 (5) ◽  
pp. 491-498 ◽  
Author(s):  
Sonja Gordic ◽  
Hatem Alkadhi ◽  
Hans-Peter Simmen ◽  
Guido Wanner ◽  
Dieter Cadosch
Keyword(s):  
Mr Imaging ◽  
Abdominal Trauma ◽  
Blunt Abdominal Trauma ◽  
Potential Role

Differential targeting of cPKC and nPKC decodes and regulates Ca2+ and lipid signalling

2014 ◽  
Vol 42 (6) ◽  
pp. 1538-1542 ◽  
Author(s):  
Xin Hui ◽  
Lars Kaestner ◽  
Peter Lipp
Keyword(s):  
Protein Kinases ◽  
Consensus Sequence ◽  
Membrane Targeting ◽  
Cellular Processes ◽  
Lipid Signalling ◽  
Pkc Isoforms

Protein kinases C (PKCs) are ubiquitously expressed and play critical roles in a plethora of physiological and pathophysiological processes. Owing to PKCs’ highly conserved phosphorylation consensus sequence, it has been difficult to distinguish the role of individual PKC isoforms. Recently, the identification of novel membrane targeting via subcellularly targeted diacylglycerol production found for novel PKCs (nPKCs), together with a characterization of their putative functions, has shed new light on the specific roles of individual PKCs in cellular processes.

scholarly journals High Intellectual Potential and High Functioning Autism: Clinical and Neurophysiological Features in a Pediatric Sample

2021 ◽  
Vol 11 (12) ◽  
pp. 1607
Author(s):  
Assia Riccioni ◽  
Stefano Pro ◽  
Lorena Di Criscio ◽  
Monica Terribili ◽  
Martina Siracusano ◽  
...  
Keyword(s):  
Potential Role ◽  
High Functioning Autism ◽  
Event Related Potentials ◽  
Adaptive Skills ◽  
High Functioning ◽  
Related Potentials ◽  
Autistic Symptoms ◽  
Intellectual Potential

High Intellectual Potential (HIP) and High Functioning Autism (HFA) are two different conditions sharing some clinical and neurobiological features. The aim of the present study was to characterize a sample of HIP children (n: 16; M/F: 14/2; median age: 10 years) in comparison to those with HFA (n: 17; M/F: 16/1; median age: 13 years) and to neurotypically developed (NTD) children (n: 10; M/F: 4/6; median age: 11 years) from a clinical and neurophysiological perspective. Specifically, a standardized clinical assessment of cognitive and adaptive skills, autistic symptoms, executive functions and behavioral features was performed. Moreover, event-related potentials (ERPs) were recorded, referring specifically to the mismatch negativity (MMN) and P300 paradigm. Our data highlighted the presence of similarities between the intellectually gifted individuals and the ones with autism (i.e., a nonhomogeneous intellective profile, an adaptive skills impairment, subthreshold autistic symptoms and increased perfectionism). Interestingly, a distinct neurophysiological characterization between groups came out, with evidence of a reduced MMN amplitude only in the HFA group. Furthermore, no differences within groups in the P300 component emerged. Therefore, our results start to provide a more informative characterization of the HIP phenotype in comparison to those of HFA and NTD, highlighting the potential role of the MMN amplitude index in helping clinicians and researchers to distinguish between HIP and HFA. Nevertheless, further research on the topic is strongly needed.

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