Cytochrome P4501A1 induction by polychlorinated biphenyls (PCBs) in liver cell lines from rat and trout and the derivation of toxic equivalency factors

1996 ◽  
Vol 53 (5) ◽  
pp. 1177-1185
Author(s):  
J H Clemons ◽  
L EJ Lee ◽  
C R Myers ◽  
D G Dixon ◽  
N C Bols
Keyword(s):  
Polychlorinated Biphenyls ◽  
Cell Lines ◽  
Liver Cell ◽  
Cytochrome P4501a1 ◽  
Equivalency Factors ◽  
Toxic Equivalency Factors

Exposure to mixtures and congeners of polychlorinated biphenyls

1994 ◽  
Vol 40 (7) ◽  
pp. 1409-1415 ◽  
Author(s):  
S Skerfving ◽  
B G Svensson ◽  
L Asplund ◽  
L Hagmar
Keyword(s):  
Polychlorinated Biphenyls ◽  
Biological Samples ◽  
Total Pcbs ◽  
Limited Information ◽  
Biomarkers Of Exposure ◽  
Equivalency Factors ◽  
Specific Analysis ◽  
Toxic Equivalency Factors ◽  
Toxic Equivalents ◽  
The Relationship

Abstract There are 209 congeners of polychlorinated biphenyls (PCBs), the metabolism and toxicity of which vary by congeners. Use of PCBs is now restricted, but environmental contamination and human exposure persist. Analysis for "total PCBs" in biological samples gives limited information; congener-specific analysis is far more informative, but more complicated. Concentrations of congeners in serum/plasma, adipose tissue, or milk are useful biomarkers of exposure. Lipids may contain similar concentrations and congener patterns, but these vary between exposures and are different from those of the corresponding exposure mixtures; hence, analysis of lipids cannot be used to identify the original exposure. Some non- and mono-ortho congeners may attain a coplanar conformation, which renders them capable of a dioxin-like action. Toxic equivalency factors (TEFs) have been used to sum that risk as toxic equivalents (TEQs), which are considerably different from congener concentrations. No reliable data have been developed on the relationship between concentrations of "total PCBs" or congeners in biological samples and effects of PCBs on human health, mainly because of the various analytical procedures involved and confounding exposures.

scholarly journals Expression of mediators of purinergic signaling in human liver cell lines

2014 ◽  
Vol 10 (4) ◽  
pp. 631-638 ◽  
Author(s):  
Jessica R. Goree ◽  
Elise G. Lavoie ◽  
Michel Fausther ◽  
Jonathan A. Dranoff
Keyword(s):  
Cell Lines ◽  
Liver Cell ◽  
Human Liver ◽  
Purinergic Signaling ◽  
Human Liver Cell

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

1978 ◽  
Vol 234 (3) ◽  
pp. C122-C130 ◽  
Author(s):  
D. M. Bissell ◽  
G. A. Levine ◽  
M. J. Bissell
Keyword(s):  
Glucose Metabolism ◽  
Primary Culture ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Time Course ◽  
Primary Cultures ◽  
Functional Change ◽  
Permanent Cell ◽  
Rat Hepatoma

The metabolic fate of [U-14C]glucose has been examined in detail in adult rat hepatocytes in primary monolayer culture, as well as in two permanent cell lines--Buffalo rat liver (BRL) and transplantable rat hepatoma (HTC) cells-derived from normal rat liver and from rat hepatoma, respectively. Under defined conditions of incubation, at a glucose concentration of 5.5 mM, the three types of cultured liver cells exhibited pronounced differences in glucose metabolism. Primary cultures, like the intact liver, differed from the cell lines in consuming relatively small amounts of glucose and converting approximately 50% of the total metabolized glucose to lactate. By contrast, the permantent cell lines consumed glucose at a 40-fold greater rate than did primary cultures, converting 80--90% of the carbohydrate to lactate. Oxidative metabolism of glucose carbon also differed among the three types of liver culture. Of the total [U-14C]glucose consumed, primary cultures converted approximately 30% to labeled CO2 per hour, whereas the liver cell lines converted 5--10%. Finally, glucose metabolism in primary culture exhibited adaptation as hepatocytes aged in culture, shifting progressively toward the pattern exhibited by the permanent cell lines. This change occurred over a time course similar to that for other kinds of functional change in hepatocytes in primary culture and thus may be relevant to the general problem of phenotypic alteration in liver cell culture.

Characterization of liver cell lines established from WHV-infected woodchucks

1995 ◽  
Vol 121 (S1) ◽  
pp. A61-A61
Author(s):  
D. Mayer ◽  
A. Loktionov ◽  
P. Bannasch
Keyword(s):  
Cell Lines ◽  
Liver Cell

Toxic equivalency factors for tetra- through octachlorinated dibenzofurans in PLHC-1 fish hepatoma cell line

1996 ◽  
Vol 42 (1-4) ◽  
pp. 401
Author(s):  
D.E. Tillitt ◽  
M. Tysklind ◽  
L. Eriksson ◽  
K. Lundgren ◽  
C. Rappe
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Equivalency Factors ◽  
Toxic Equivalency Factors
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